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The map label for this gene is leuB [H]

Identifier: 153949839

GI number: 153949839

Start: 3831356

End: 3832447

Strand: Direct

Name: leuB [H]

Synonym: YpsIP31758_3406

Alternate gene names: 153949839

Gene position: 3831356-3832447 (Clockwise)

Preceding gene: 153948574

Following gene: 153949465

Centisome position: 81.12

GC content: 50.82

Gene sequence:

>1092_bases
ATGACGAAGACTTATCATATTGCAGTGTTGCCCGGCGACGGTATCGGCCCTGAAGTAATGGATCAGGCAAGTAAAGTATT
GGATGCGGTGCGTCAGCGCTTCGGTCTCAAGATTTCAACCAGTGTTTACGATGTTGGTGGCGCTGCCATTGACCGCCATG
GTAGCCCATTACCGGCAGCAACGGTCGCGGGATGTGAACAGGCAGATGCCATTTTATTTGGCTCCGTAGGCGGCCCTAAA
TGGGAACACCTGCCACCAGCAGAACAACCTGAGCGTGGCGCATTATTGCCCTTGCGTAAACATTTCAAATTATTCAGTAA
CTTGCGCCCTGCCCGTTTGTATCAAGGATTGGAGGACTTCTGTCCATTGCGCAGTGATATCGCGGCCAGAGGTTTTGATA
TCCTGTGTGTCCGTGAATTAACCGGCGGTATCTATTTCGGCCAGCCGAAAGGCCGTGAAGGCCAAGGCATGCACGAGCGG
GCCTTTGACACCGAAGTTTATCATCGCTTCGAAATTGAACGTATCGCCCGTATTGCTTTCGAATCAGCCCGCAAACGCCG
TAGTAAAGTGACGTCCATTGATAAAGCCAACGTGTTGCAAAGCTCAATCTTGTGGCGTGAAGTGGTCAACGGGATCGTGG
CAGATTACCCGGATGTTGCGTTGTCCCATATGTACATTGATAACGCCACCATGCAGTTAATCAAAGACCCTTCCCAATTT
GACGTGTTGCTGTGCTCTAACTTGTTTGGCGATATCTTGTCCGATGAATGCGCCATGATCACCGGCTCCATGGGCATGTT
GCCATCAGCCAGTTTGAATGAACAAGGTTTTGGTCTATATGAACCTGCAGGCGGTTCCGCACCGGATATCGCGGGTAAGA
ACATTGCCAACCCTATCGCTCAGATCCTGTCATTAACCCTGTTGCTGCGTTTTAGCTTAGGTAAAGATGATGCCGCTGAC
GCCATTGAGCGCGCGATCAATCAGGCATTGGAGCAAGGTTATCGTACTGCGGATTTAGCCGGGGATGGTCACGCAATCGG
CACTCACGAAATGGGCGATATCATCGCTAAATTCGTGGTTGAGGGGGTATAA

Upstream 100 bases:

>100_bases
CTAAGGCGTTGGTTCACGTATTAAATAACATCTGGCGCGCCCATCAGGTCGAAAAAGAGAAGCAGCGTCTGCAACAAAAT
AATCAGGAAATGGTGTGAAC

Downstream 100 bases:

>100_bases
AATGGGCACAACATCATCTCAGAGCAAAACGTTATACCAAAAATTGTACGATGCGCACATTGTGCATGAAGCCCCCAATG
AGACCCCATTGCTGTATATC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 363; Mature: 362

Protein sequence:

>363_residues
MTKTYHIAVLPGDGIGPEVMDQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIVADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Sequences:

>Translated_363_residues
MTKTYHIAVLPGDGIGPEVMDQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPK
WEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHER
AFDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIVADYPDVALSHMYIDNATMQLIKDPSQF
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAAD
AIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV
>Mature_362_residues
TKTYHIAVLPGDGIGPEVMDQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAATVAGCEQADAILFGSVGGPKW
EHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDFCPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERA
FDTEVYHRFEIERIARIAFESARKRRSKVTSIDKANVLQSSILWREVVNGIVADYPDVALSHMYIDNATMQLIKDPSQFD
VLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIAQILSLTLLLRFSLGKDDAADA
IERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVVEGV

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=341, Percent_Identity=32.8445747800587, Blast_Score=154, Evalue=2e-37,
Organism=Homo sapiens, GI4758582, Length=351, Percent_Identity=27.6353276353276, Blast_Score=110, Evalue=2e-24,
Organism=Homo sapiens, GI28178821, Length=315, Percent_Identity=28.2539682539683, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI28178816, Length=312, Percent_Identity=27.2435897435897, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI28178838, Length=331, Percent_Identity=26.2839879154079, Blast_Score=105, Evalue=8e-23,
Organism=Homo sapiens, GI28178819, Length=150, Percent_Identity=36, Blast_Score=96, Evalue=6e-20,
Organism=Escherichia coli, GI87081683, Length=363, Percent_Identity=85.6749311294766, Blast_Score=644, Evalue=0.0,
Organism=Escherichia coli, GI1788101, Length=371, Percent_Identity=38.2749326145553, Blast_Score=206, Evalue=3e-54,
Organism=Escherichia coli, GI1787381, Length=398, Percent_Identity=26.1306532663317, Blast_Score=82, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI71986051, Length=342, Percent_Identity=32.1637426900585, Blast_Score=152, Evalue=4e-37,
Organism=Caenorhabditis elegans, GI25144293, Length=343, Percent_Identity=29.4460641399417, Blast_Score=127, Evalue=6e-30,
Organism=Caenorhabditis elegans, GI17505779, Length=352, Percent_Identity=29.8295454545455, Blast_Score=125, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=345, Percent_Identity=28.695652173913, Blast_Score=117, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6319830, Length=370, Percent_Identity=42.7027027027027, Blast_Score=266, Evalue=5e-72,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=33.8068181818182, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6324709, Length=347, Percent_Identity=30.835734870317, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6324291, Length=343, Percent_Identity=29.7376093294461, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24643270, Length=341, Percent_Identity=32.258064516129, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24643268, Length=341, Percent_Identity=32.258064516129, Blast_Score=160, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24661184, Length=344, Percent_Identity=29.0697674418605, Blast_Score=129, Evalue=2e-30,
Organism=Drosophila melanogaster, GI161078637, Length=339, Percent_Identity=29.4985250737463, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI161078635, Length=339, Percent_Identity=29.4985250737463, Blast_Score=121, Evalue=7e-28,
Organism=Drosophila melanogaster, GI161078633, Length=339, Percent_Identity=29.2035398230088, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24650122, Length=339, Percent_Identity=29.2035398230088, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI161078639, Length=337, Percent_Identity=29.673590504451, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI281362242, Length=340, Percent_Identity=26.7647058823529, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24648872, Length=340, Percent_Identity=26.7647058823529, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI20130355, Length=344, Percent_Identity=24.4186046511628, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 39339; Mature: 39208

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTYHIAVLPGDGIGPEVMDQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEEECCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIVADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHH
QILSLTLLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC
>Mature Secondary Structure 
TKTYHIAVLPGDGIGPEVMDQASKVLDAVRQRFGLKISTSVYDVGGAAIDRHGSPLPAA
CCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCEEECCEEECCCHHHCCCCCCCCHH
TVAGCEQADAILFGSVGGPKWEHLPPAEQPERGALLPLRKHFKLFSNLRPARLYQGLEDF
HHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCHHHHHHCHHHH
CPLRSDIAARGFDILCVRELTGGIYFGQPKGREGQGMHERAFDTEVYHRFEIERIARIAF
CCCHHHHHHCCCCEEEEEHHHCCEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
ESARKRRSKVTSIDKANVLQSSILWREVVNGIVADYPDVALSHMYIDNATMQLIKDPSQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHCCCCHH
DVLLCSNLFGDILSDECAMITGSMGMLPSASLNEQGFGLYEPAGGSAPDIAGKNIANPIA
HHHHHHHHHHHHHCCCHHEEECCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCHHHHHHH
QILSLTLLLRFSLGKDDAADAIERAINQALEQGYRTADLAGDGHAIGTHEMGDIIAKFVV
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCEEECCCCCCCCCCHHHHHHHHHHHH
EGV
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]