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The map label for this gene is cafA [H]

Identifier: 153949561

GI number: 153949561

Start: 462178

End: 463647

Strand: Direct

Name: cafA [H]

Synonym: YpsIP31758_0402

Alternate gene names: 153949561

Gene position: 462178-463647 (Clockwise)

Preceding gene: 153948992

Following gene: 153948116

Centisome position: 9.79

GC content: 47.69

Gene sequence:

>1470_bases
ATGACAGCTGAATTACTGGTCAATATTACACCGTCTGAAACGCGTGTTGCTTATATTGATGGCGGCATCCTGCAAGAAAT
ACATATCGAGCGTGAAGCCAAAAGAGGGATCGTTGGCAATATTTATAAAGGGCGAGTCAGCCGGGTTTTACCGGGTATGC
AAGCTGCTTTTGTTGACATAGGTCTGGATAAAGCCGCTTTCCTCCATGCTTCCGATATCATGCCCCACACCGAATGCGTG
GCCGGGGATGAACAGAAAAACTTCAATGTGCGCGATATCGCCGAACTGGTCCGTCAGGGGCAGGATTTAATGGTACAAGT
GGTGAAAGATCCCTTGGGTACCAAAGGCGCACGTTTGACGACCGATATTACTCTTCCTTCTCGCTATCTGGTGTTAATGC
CCGGAGCTGCCCATGTTGGGGTTTCCCAGCGGATTGAAAGTGAAGCAGAGCGCGAACGCCTAAAAAAGACGGTTGCGGCT
TACTGTGATGAGCAGGGCGGTTTTATTATTCGTACTGCCGCTGAAGGGATTGGCGAAGAAGAGCTATCCGCTGATGCCGC
GTTCCTTAAACGTTTGTGGACCAAAGTTCAGGAGCGTAAGAAGCGTAATATCACCAAATATAAGCTGTATGGTGAAATGG
CGCTGGCGCAACGCGTATTGCGTGATTTTGCTGGTGCGGCGCTGGATAAAATCCGCGTTGACTCAAAATTGACTTACGAT
TTGCTGGTGGAGTTCACCCGCGAATATATTCCAGAAATGACGGACAAGCTGGAGCTGTATACTGGGAAGCAACCTATTTT
CGATCTCTATGATGTCGAGAATGAAATTCAGCGCTCTCTGGAGCGTAAAGTTGAATTAAAATCAGGTGGTTATCTGATTA
TCGATCAGACAGAAGCGATGACTACCGTGGATATTAACACTGGGGCATTTGTCGGGCATCGGAATCTTGAGGAAACCATT
TTCAATACCAATATTGAGGCGACGCAGGCCATTGCCCGCCAATTGCGGATGCGTAATCTGGGCGGGATTATCATTATTGA
TTTCATTGATATGAGTAATGAAGATCATCGCCGGCGGGTATTGCATTCATTGGAACAAGCACTGAGTAAAGATCGCGTAA
AAACCAGTATTAACGGCTTCTCCCAGCTAGGCTTAGTCGAAATGACGCGTAAGCGTACGCGTGAAAGTATTGAGCATGTT
TTATGCCATGAATGCCCAACCTGCCGTGGCCGTGGCACGGTAAAATCCGTAGAGACCGTGTGCTACGAAATCTTGCGTGA
AATTGTCCGTGTTCATCATGCTTATGATTCTGACCGTTTCTTGGTCTATGCTTCTCCGGCTGTAGGTGAGGCGCTGAAAG
GCGAAGAGTCTCATGCACTGGCCGAGGTGGAAATCTTCGTCGGTAAGCAGGTAAAAGTTCAGATAGAGCCACTGTATAAC
CAAGAACAGTTTGACGTGGTGATGATGTAA

Upstream 100 bases:

>100_bases
GCCATTGCAGGCAGTTATCACGCAGTGGTGGGCCTGCCGTTAGTTGAAACCCATGAATTACTAAGTCATTTTATTGCGCA
GCGTAATGTGAGAGGAATAC

Downstream 100 bases:

>100_bases
GGGCCTTCCCGTGTTGTTGGCGAAGGTTCTAAGACGGTTCCGCTGTGGCCCTTGCAGCGTCATGGTCTCACTGCGGATTG
AAAGGAGAAAGGCGTGAGGC

Product: ribonuclease G

Products: NA

Alternate protein names: RNase G; Cytoplasmic axial filament protein [H]

Number of amino acids: Translated: 489; Mature: 488

Protein sequence:

>489_residues
MTAELLVNITPSETRVAYIDGGILQEIHIEREAKRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGDEQKNFNVRDIAELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVLMPGAAHVGVSQRIESEAERERLKKTVAA
YCDEQGGFIIRTAAEGIGEEELSADAAFLKRLWTKVQERKKRNITKYKLYGEMALAQRVLRDFAGAALDKIRVDSKLTYD
LLVEFTREYIPEMTDKLELYTGKQPIFDLYDVENEIQRSLERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLEETI
FNTNIEATQAIARQLRMRNLGGIIIIDFIDMSNEDHRRRVLHSLEQALSKDRVKTSINGFSQLGLVEMTRKRTRESIEHV
LCHECPTCRGRGTVKSVETVCYEILREIVRVHHAYDSDRFLVYASPAVGEALKGEESHALAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM

Sequences:

>Translated_489_residues
MTAELLVNITPSETRVAYIDGGILQEIHIEREAKRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECV
AGDEQKNFNVRDIAELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVLMPGAAHVGVSQRIESEAERERLKKTVAA
YCDEQGGFIIRTAAEGIGEEELSADAAFLKRLWTKVQERKKRNITKYKLYGEMALAQRVLRDFAGAALDKIRVDSKLTYD
LLVEFTREYIPEMTDKLELYTGKQPIFDLYDVENEIQRSLERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLEETI
FNTNIEATQAIARQLRMRNLGGIIIIDFIDMSNEDHRRRVLHSLEQALSKDRVKTSINGFSQLGLVEMTRKRTRESIEHV
LCHECPTCRGRGTVKSVETVCYEILREIVRVHHAYDSDRFLVYASPAVGEALKGEESHALAEVEIFVGKQVKVQIEPLYN
QEQFDVVMM
>Mature_488_residues
TAELLVNITPSETRVAYIDGGILQEIHIEREAKRGIVGNIYKGRVSRVLPGMQAAFVDIGLDKAAFLHASDIMPHTECVA
GDEQKNFNVRDIAELVRQGQDLMVQVVKDPLGTKGARLTTDITLPSRYLVLMPGAAHVGVSQRIESEAERERLKKTVAAY
CDEQGGFIIRTAAEGIGEEELSADAAFLKRLWTKVQERKKRNITKYKLYGEMALAQRVLRDFAGAALDKIRVDSKLTYDL
LVEFTREYIPEMTDKLELYTGKQPIFDLYDVENEIQRSLERKVELKSGGYLIIDQTEAMTTVDINTGAFVGHRNLEETIF
NTNIEATQAIARQLRMRNLGGIIIIDFIDMSNEDHRRRVLHSLEQALSKDRVKTSINGFSQLGLVEMTRKRTRESIEHVL
CHECPTCRGRGTVKSVETVCYEILREIVRVHHAYDSDRFLVYASPAVGEALKGEESHALAEVEIFVGKQVKVQIEPLYNQ
EQFDVVMM

Specific function: Involved in the processing of the 5'-end of 16S rRNA. Could be involved in chromosome segregation and cell division. It may be one of the components of the cytoplasmic axial filaments bundles or merely regulate the formation of this structure [H]

COG id: COG1530

COG function: function code J; Ribonucleases G and E

Gene ontology:

Cell location: Cytoplasm, cytoskeleton [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI87082235, Length=489, Percent_Identity=90.1840490797546, Blast_Score=899, Evalue=0.0,
Organism=Escherichia coli, GI1787325, Length=436, Percent_Identity=37.6146788990826, Blast_Score=285, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR019307
- InterPro:   IPR022967
- InterPro:   IPR004659 [H]

Pfam domain/function: PF10150 RNase_E_G; PF00575 S1 [H]

EC number: 3.1.4.- [C]

Molecular weight: Translated: 55246; Mature: 55115

Theoretical pI: Translated: 6.13; Mature: 6.13

Prosite motif: PS50126 S1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAELLVNITPSETRVAYIDGGILQEIHIEREAKRGIVGNIYKGRVSRVLPGMQAAFVDI
CCCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCC
GLDKAAFLHASDIMPHTECVAGDEQKNFNVRDIAELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVLMPGAAHVGVSQRIESEAERERLKKTVAAYCDEQGGFIIRTAAEGIGEE
EEEECCCCEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
ELSADAAFLKRLWTKVQERKKRNITKYKLYGEMALAQRVLRDFAGAALDKIRVDSKLTYD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLVEFTREYIPEMTDKLELYTGKQPIFDLYDVENEIQRSLERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHCEEEEECCCCCHHHHCCHHHHHHHHHHHEEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLEETIFNTNIEATQAIARQLRMRNLGGIIIIDFIDMSNEDHRRRV
EEEECCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
LHSLEQALSKDRVKTSINGFSQLGLVEMTRKRTRESIEHVLCHECPTCRGRGTVKSVETV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEILREIVRVHHAYDSDRFLVYASPAVGEALKGEESHALAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCEEEEEC
>Mature Secondary Structure 
TAELLVNITPSETRVAYIDGGILQEIHIEREAKRGIVGNIYKGRVSRVLPGMQAAFVDI
CCEEEEEECCCCCEEEEECCCHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHCC
GLDKAAFLHASDIMPHTECVAGDEQKNFNVRDIAELVRQGQDLMVQVVKDPLGTKGARLT
CCCHHHHEEHHCCCCCHHHCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCCCCCCCEEE
TDITLPSRYLVLMPGAAHVGVSQRIESEAERERLKKTVAAYCDEQGGFIIRTAAEGIGEE
EEEECCCCEEEEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHH
ELSADAAFLKRLWTKVQERKKRNITKYKLYGEMALAQRVLRDFAGAALDKIRVDSKLTYD
HHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHH
LLVEFTREYIPEMTDKLELYTGKQPIFDLYDVENEIQRSLERKVELKSGGYLIIDQTEAM
HHHHHHHHHHHHHHHCEEEEECCCCCHHHHCCHHHHHHHHHHHEEECCCCEEEEECCCCE
TTVDINTGAFVGHRNLEETIFNTNIEATQAIARQLRMRNLGGIIIIDFIDMSNEDHRRRV
EEEECCCCCEEECCCHHHHHHCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCHHHHHH
LHSLEQALSKDRVKTSINGFSQLGLVEMTRKRTRESIEHVLCHECPTCRGRGTVKSVETV
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHH
CYEILREIVRVHHAYDSDRFLVYASPAVGEALKGEESHALAEVEIFVGKQVKVQIEPLYN
HHHHHHHHHHHHHCCCCCCEEEEECCCHHHHHCCCCCCCEEEEEEEECCEEEEEEEECCC
QEQFDVVMM
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]