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The map label for this gene is mdtK [H]

Identifier: 153949438

GI number: 153949438

Start: 2025923

End: 2027296

Strand: Reverse

Name: mdtK [H]

Synonym: YpsIP31758_1750

Alternate gene names: 153949438

Gene position: 2027296-2025923 (Counterclockwise)

Preceding gene: 153950563

Following gene: 153948891

Centisome position: 42.92

GC content: 51.16

Gene sequence:

>1374_bases
GTGCAGAAGTATATAGTTGAAGCGCGTAGCTTGTTGGCTCTCGCTATTCCCGTTGTCATCGCGCAACTGTCTCAAACCGC
TATGGGTGTTGTTGACACGATCATGGCCGGCTCTGTCAGTGCAACAGACATGGCAGCGGTGGCGGTAGGCACGTCTATCT
GGCTACCCGCTATTTTATTCGGACACGGCTTGTTACTCGCACTAACGCCGACTGTCGCCCAGCTTAATGGTTCAGGGCGC
CGTAGCCAGATTGCCCACCAAGTCAGGCAAGGGTTTTGGCTGGCACTCTGTGTTTCCGTGTTAATTATGCTGGTGCTCTA
TAATAGTGACCACGTTATTAAACAAATGGATAATATTGACCCCGTGCTGGCGCAGAAAGCCGTGGGTTTTCTGCATGCTA
TCATGTGGGGTGTGCCCGGTTATCTGTTCTTCCAAGTTCTGAGAAATCAGTGTGAAGGGCTATCCAAGACTAAACCGGGA
ATGGTTATCGGGTTTGTTGGCCTGCTGGTTAACATCCCGATTAACTACATTTTTATCTATGGGAAATTTGGCGCGCCGGC
CTTAGGTGGCGTAGGCTGCGGGGTGGCAACAGCCTCTGTTTATTGGGTGATGTTCCTGATGATGAGATGGTACGTAACGC
GCGCCCGTTCTCAACAGGATATCAAACTGGAAAAAGGATTTGCCGCGCCCGATTGGCAGGTAATGAAGCGTTTAAGTGGC
CTTGGCTTGCCCGTCGCCTTGGCCCTTTTTTTCGAGGTCACGCTGTTCGCCGTCGTCGCCCTATTGGTATCGCCACTGGG
TATTGTTGCTGTTGCCGGACACCAGATTGCCCTCAACTTCAGTTCATTGATGTTTATGTTACCCATGTCACTGAGTGTAG
CAGCGACAATTCGTGTCGGGTTTCGTCTCGGACAAGGGTCGGTGGAACAGGCTCAGGTTGCAGCCTATACCAGCATGGCG
GTTGGTTTATTGCTGGCCAGCGTTACCGCTGTGTTTACCATTGTTTTCCGTGAACATATTGCGTTGTTGTATAACAAGAC
ACCTGAAGTGGTTACCATGGCGTCCCATCTGATGTTGCTGGCGGCACTTTATCAGTTGTCTGATGCCGTTCAGGTTATTG
GGAGTGGTGTCTTGCGGGGCTATAAAGATACGCGCTCCATTTTCTTTATTACGTTTACCGCGTATTGGCTTCTCGGGTTA
CCCAGCGGCTACCTTCTGGGCTTGACCGATTATATCCTCCCTGCGATGGGGCCTGCTGGTTTCTGGATAGGTTTCATTAT
CGGTCTGACGGCTGCGGCTATTCTGATGGTGCTGCGTATCCGTTGGCTACAAAAACAACCTACTGCGTTTATTCTGCAAC
GGGCAGCCCATTAG

Upstream 100 bases:

>100_bases
GGTGAGCAGGTACAATATTTTATTGCTTTCCCACTATTTTATTTATCATCGCCTGATGATAAACCGTCATGATTTAAAAC
ATAATTAAAAAGGTGCATAC

Downstream 100 bases:

>100_bases
CTTATCGTGGAGTGGGGCTACCCCCCGCTCCGCCATAAAACACCTGTAGGGGCTTCGCGCGGTTAGTCTCAAATAAAAGG
GAGTCTCCCCAACAACATCT

Product: multidrug efflux protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: Multidrug-efflux transporter [H]

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MQKYIVEARSLLALAIPVVIAQLSQTAMGVVDTIMAGSVSATDMAAVAVGTSIWLPAILFGHGLLLALTPTVAQLNGSGR
RSQIAHQVRQGFWLALCVSVLIMLVLYNSDHVIKQMDNIDPVLAQKAVGFLHAIMWGVPGYLFFQVLRNQCEGLSKTKPG
MVIGFVGLLVNIPINYIFIYGKFGAPALGGVGCGVATASVYWVMFLMMRWYVTRARSQQDIKLEKGFAAPDWQVMKRLSG
LGLPVALALFFEVTLFAVVALLVSPLGIVAVAGHQIALNFSSLMFMLPMSLSVAATIRVGFRLGQGSVEQAQVAAYTSMA
VGLLLASVTAVFTIVFREHIALLYNKTPEVVTMASHLMLLAALYQLSDAVQVIGSGVLRGYKDTRSIFFITFTAYWLLGL
PSGYLLGLTDYILPAMGPAGFWIGFIIGLTAAAILMVLRIRWLQKQPTAFILQRAAH

Sequences:

>Translated_457_residues
MQKYIVEARSLLALAIPVVIAQLSQTAMGVVDTIMAGSVSATDMAAVAVGTSIWLPAILFGHGLLLALTPTVAQLNGSGR
RSQIAHQVRQGFWLALCVSVLIMLVLYNSDHVIKQMDNIDPVLAQKAVGFLHAIMWGVPGYLFFQVLRNQCEGLSKTKPG
MVIGFVGLLVNIPINYIFIYGKFGAPALGGVGCGVATASVYWVMFLMMRWYVTRARSQQDIKLEKGFAAPDWQVMKRLSG
LGLPVALALFFEVTLFAVVALLVSPLGIVAVAGHQIALNFSSLMFMLPMSLSVAATIRVGFRLGQGSVEQAQVAAYTSMA
VGLLLASVTAVFTIVFREHIALLYNKTPEVVTMASHLMLLAALYQLSDAVQVIGSGVLRGYKDTRSIFFITFTAYWLLGL
PSGYLLGLTDYILPAMGPAGFWIGFIIGLTAAAILMVLRIRWLQKQPTAFILQRAAH
>Mature_457_residues
MQKYIVEARSLLALAIPVVIAQLSQTAMGVVDTIMAGSVSATDMAAVAVGTSIWLPAILFGHGLLLALTPTVAQLNGSGR
RSQIAHQVRQGFWLALCVSVLIMLVLYNSDHVIKQMDNIDPVLAQKAVGFLHAIMWGVPGYLFFQVLRNQCEGLSKTKPG
MVIGFVGLLVNIPINYIFIYGKFGAPALGGVGCGVATASVYWVMFLMMRWYVTRARSQQDIKLEKGFAAPDWQVMKRLSG
LGLPVALALFFEVTLFAVVALLVSPLGIVAVAGHQIALNFSSLMFMLPMSLSVAATIRVGFRLGQGSVEQAQVAAYTSMA
VGLLLASVTAVFTIVFREHIALLYNKTPEVVTMASHLMLLAALYQLSDAVQVIGSGVLRGYKDTRSIFFITFTAYWLLGL
PSGYLLGLTDYILPAMGPAGFWIGFIIGLTAAAILMVLRIRWLQKQPTAFILQRAAH

Specific function: Multidrug efflux pump that functions probably as a Na(+)/drug antiporter [H]

COG id: COG0534

COG function: function code V; Na+-driven multidrug efflux pump

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family. MdtK subfamily [H]

Homologues:

Organism=Homo sapiens, GI22907060, Length=445, Percent_Identity=23.3707865168539, Blast_Score=98, Evalue=1e-20,
Organism=Homo sapiens, GI153792564, Length=442, Percent_Identity=20.1357466063348, Blast_Score=79, Evalue=9e-15,
Organism=Escherichia coli, GI48994890, Length=457, Percent_Identity=75.9299781181619, Blast_Score=696, Evalue=0.0,
Organism=Escherichia coli, GI226510954, Length=447, Percent_Identity=24.6085011185682, Blast_Score=96, Evalue=6e-21,
Organism=Saccharomyces cerevisiae, GI6321821, Length=261, Percent_Identity=25.6704980842912, Blast_Score=84, Evalue=4e-17,
Organism=Saccharomyces cerevisiae, GI6320545, Length=435, Percent_Identity=19.3103448275862, Blast_Score=78, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002528
- InterPro:   IPR022913 [H]

Pfam domain/function: PF01554 MatE [H]

EC number: NA

Molecular weight: Translated: 49418; Mature: 49418

Theoretical pI: Translated: 10.02; Mature: 10.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQKYIVEARSLLALAIPVVIAQLSQTAMGVVDTIMAGSVSATDMAAVAVGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPTVAQLNGSGRRSQIAHQVRQGFWLALCVSVLIMLVLYNSDHVIKQMDNID
HCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
PVLAQKAVGFLHAIMWGVPGYLFFQVLRNQCEGLSKTKPGMVIGFVGLLVNIPINYIFIY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEE
GKFGAPALGGVGCGVATASVYWVMFLMMRWYVTRARSQQDIKLEKGFAAPDWQVMKRLSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHC
LGLPVALALFFEVTLFAVVALLVSPLGIVAVAGHQIALNFSSLMFMLPMSLSVAATIRVG
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
FRLGQGSVEQAQVAAYTSMAVGLLLASVTAVFTIVFREHIALLYNKTPEVVTMASHLMLL
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AALYQLSDAVQVIGSGVLRGYKDTRSIFFITFTAYWLLGLPSGYLLGLTDYILPAMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWIGFIIGLTAAAILMVLRIRWLQKQPTAFILQRAAH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MQKYIVEARSLLALAIPVVIAQLSQTAMGVVDTIMAGSVSATDMAAVAVGTSIWLPAILF
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
GHGLLLALTPTVAQLNGSGRRSQIAHQVRQGFWLALCVSVLIMLVLYNSDHVIKQMDNID
HCCHHHHCCHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCC
PVLAQKAVGFLHAIMWGVPGYLFFQVLRNQCEGLSKTKPGMVIGFVGLLVNIPINYIFIY
HHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCEEEEEEE
GKFGAPALGGVGCGVATASVYWVMFLMMRWYVTRARSQQDIKLEKGFAAPDWQVMKRLSG
ECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCCCHHHHHHHHC
LGLPVALALFFEVTLFAVVALLVSPLGIVAVAGHQIALNFSSLMFMLPMSLSVAATIRVG
CCHHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
FRLGQGSVEQAQVAAYTSMAVGLLLASVTAVFTIVFREHIALLYNKTPEVVTMASHLMLL
EEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHH
AALYQLSDAVQVIGSGVLRGYKDTRSIFFITFTAYWLLGLPSGYLLGLTDYILPAMGPAG
HHHHHHHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHHHCCCCHHHHHHHHHHHCCCCHH
FWIGFIIGLTAAAILMVLRIRWLQKQPTAFILQRAAH
HHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA