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The map label for this gene is hmp [H]

Identifier: 153949368

GI number: 153949368

Start: 1361493

End: 1362683

Strand: Reverse

Name: hmp [H]

Synonym: YpsIP31758_1157

Alternate gene names: 153949368

Gene position: 1362683-1361493 (Counterclockwise)

Preceding gene: 153947660

Following gene: 153948958

Centisome position: 28.85

GC content: 47.02

Gene sequence:

>1191_bases
ATGCTGGATGCCCAAACCATCGCTATCGTTAAGTCTACCATTCCCTTGCTGGCTGCCACCGGCCCCAAACTGACAGCCCA
TTTCTATGAGCGCATGTTTAAGCATCACCCTGAACTCAAACACATTTTTAATATGAGTAACCAATCGAGTGGTGATCAAC
GCGAAGCCTTATTTAATGCTATTTGTGCTTATGCGACCAATATCGAAAATCTTGCGGCACTACTCCCCACGGTTGAACGT
ATTGCCCAAAAACATACCAGTTTAAATATTCAGCCAGAGCATTACCCTATTGTTGGTGAGCATTTAATCGCTACCCTTGA
TGAACTATTTTCACCCGGTCAGGCCGTTTTAGATGCCTGGGCTAAAGCCTATGGTGTCTTGGCTGATGTGTTTATTCAGC
GTGAAAGCCAGATTTATCAGCAAAGTGAGACGGAAACCGGTGGTTGGAGAACCTTACGCCGCTTCCGCATTATAAAAAAA
GAGCAACAAAGCGAGGTAATTTGCAGTTTTGTCTTGGCACCGGAAGACGGCGGCCAAGTGCTTCACTATAAACCGGGGCA
ATATTTAGGCATTTATATCGAGCATGAAAGCCTTGAATTCCAAGAGATTCGTCAATATTCGCTGACCACCGCGCCCAATG
GCAAAACTTACCGTATTGCAGTAAAACGTGAAGAACAAGGCACGGTTTCAAACTTACTGCATCGGGAGTTAAATGAAGGG
GATATCGTGCGCATCGCACCACCCCGTGGCGATTTCTTTTTGGATGTATCACCAGATACCCCGGTAGCACTGATTTCTGC
TGGGGTGGGCCAAACCCCGATGCTCAGTATGCTCAATACCCTCTATAGCCAACAACATGCTGCCCCCGTACATTGGTTAC
ACGCAGCTGAAAATGGTCGTGTACACGCATTCGCTGATGAAGTTAGCGCTATCGCAGCCAAGATGCCTAATCTAAGCCGC
CATGTGTGGTACCGCGAGCCAGACCTACAGGATAAACACGGCGAGGATTACCATAGCCAAGGGTTGATGGATCTAAGTTC
GTACCAGTGGCTAGCTGATGATCCTAAACGACATTATTACTTCTGTGGCCCGCTGCCATTTATGCAATTTATAGGTCGTC
AATTACTGGCCCAAGGGATAGCACCTGAGCAGATTCACTACGAATGCTTCGGTCCACATAAAGTCATTTAA

Upstream 100 bases:

>100_bases
ACAGGTGAAAAGTACATTTACAAAGAGAAGAGAAAACCATAAGATGCATTTAAAATGCATGTATTGATTTGTATATTATT
AAAACACTTAAGGAGCCACC

Downstream 100 bases:

>100_bases
CTGTTCATTTTTATCCTATCTCTACGCACTTAATGCCGCTGATCAGGAAGGTACCTGATCAGCATTTCGGCTATTTCAAT
TCCCCCATTTATTACATTTA

Product: nitric oxide dioxygenase

Products: NA

Alternate protein names: Flavohemoglobin; Hemoglobin-like protein; Nitric oxide dioxygenase; NO oxygenase; NOD [H]

Number of amino acids: Translated: 396; Mature: 396

Protein sequence:

>396_residues
MLDAQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKHIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI

Sequences:

>Translated_396_residues
MLDAQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKHIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
>Mature_396_residues
MLDAQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKHIFNMSNQSSGDQREALFNAICAYATNIENLAALLPTVER
IAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAWAKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKK
EQQSEVICSFVLAPEDGGQVLHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGRVHAFADEVSAIAAKMPNLSR
HVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYYFCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI

Specific function: Is involved in NO detoxification in an aerobic process, termed nitric oxide dioxygenase (NOD) reaction that utilizes O(2) and NAD(P)H to convert NO to nitrate, which protects the bacterium from various noxious nitrogen compounds. Therefore, plays a centra

COG id: COG1018

COG function: function code C; Flavodoxin reductases (ferredoxin-NADPH reductases) family 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI1788903, Length=396, Percent_Identity=63.3838383838384, Blast_Score=537, Evalue=1e-154,
Organism=Escherichia coli, GI1787098, Length=201, Percent_Identity=22.8855721393035, Blast_Score=64, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321673, Length=413, Percent_Identity=31.7191283292978, Blast_Score=200, Evalue=3e-52,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001709
- InterPro:   IPR012292
- InterPro:   IPR009050
- InterPro:   IPR000971
- InterPro:   IPR008333
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00970 FAD_binding_6; PF00042 Globin; PF00175 NAD_binding_1 [H]

EC number: =1.14.12.17 [H]

Molecular weight: Translated: 44809; Mature: 44809

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: PS01033 GLOBIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDAQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKHIFNMSNQSSGDQREALFNA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ICAYATNIENLAALLPTVERIAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAW
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
AKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKKEQQSEVICSFVLAPEDGGQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEE
LHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
EEECCCCEEEEEEEECCCCHHHHHHHHCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCC
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGR
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
VHAFADEVSAIAAKMPNLSRHVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYY
EEEHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCEE
FCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC
>Mature Secondary Structure
MLDAQTIAIVKSTIPLLAATGPKLTAHFYERMFKHHPELKHIFNMSNQSSGDQREALFNA
CCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCHHHHHHCCCCCCCCHHHHHHHHH
ICAYATNIENLAALLPTVERIAQKHTSLNIQPEHYPIVGEHLIATLDELFSPGQAVLDAW
HHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHCCCHHHHHHH
AKAYGVLADVFIQRESQIYQQSETETGGWRTLRRFRIIKKEQQSEVICSFVLAPEDGGQV
HHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCEE
LHYKPGQYLGIYIEHESLEFQEIRQYSLTTAPNGKTYRIAVKREEQGTVSNLLHRELNEG
EEECCCCEEEEEEEECCCCHHHHHHHHCEECCCCCEEEEEEEECCCCHHHHHHHHHCCCC
DIVRIAPPRGDFFLDVSPDTPVALISAGVGQTPMLSMLNTLYSQQHAAPVHWLHAAENGR
CEEEEECCCCCEEEEECCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCCEEEEEECCCCC
VHAFADEVSAIAAKMPNLSRHVWYREPDLQDKHGEDYHSQGLMDLSSYQWLADDPKRHYY
EEEHHHHHHHHHHHCCCCHHHEEECCCCCCCCCCCCHHHCCCCCHHCCCCCCCCCCCCEE
FCGPLPFMQFIGRQLLAQGIAPEQIHYECFGPHKVI
EECCHHHHHHHHHHHHHHCCCCCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]