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The map label for this gene is aceF [H]

Identifier: 153949314

GI number: 153949314

Start: 3778297

End: 3779877

Strand: Reverse

Name: aceF [H]

Synonym: YpsIP31758_3362

Alternate gene names: 153949314

Gene position: 3779877-3778297 (Counterclockwise)

Preceding gene: 153949881

Following gene: 153949037

Centisome position: 80.03

GC content: 52.37

Gene sequence:

>1581_bases
ATGTCTATAGAAATTAATGTACCAGACATCGGTGCAGATGAAGTGGAAGTCACCGAAATTATGGTGAAAGTGGGCGATAC
CGTTGAAGCGGAACAGTCGCTAATCACCGTTGAAGGCGATAAAGCTTCCATGGAAGTTCCTTCACCTCAGGCGGGCGTGG
TTAAAGAGATCAAAATTGCGGTTGGCGATAAAGTGGCTACCGGTTCTCTGATCATGGTCTTCGACGCTACGGGTGCCGCT
GCGGCACCGGTTAAAGCAGAAGAAAAACCGGCGGCGCCTGCTCAGGCAGCGGCTCCGGCAGCCTCTGCGGCGAAAAATGT
TGAAGTGCCAGATATCGGTGATGACGAAGTTGAAGTGACTGAAGTGATGGTGAAAGTGGGCGATAAAGTTGACGCCGAAC
AATCACTGATTACGGTTGAAGGCGACAAAGCGTCGATGGAAGTGCCCGCACCGTTTGCTGGTATCGTGAAAGAAATCAAA
ATCAGTACCGGCGACAAAGTGAAAACCGGCTCTCTGATTATGGTCTTCGAAGTTGAAGGTGCAGCGCCAGCGCCAGCAGC
AGAAGCGGCTCCGGCTCAACAAGCCGCACCTGTCGCGCCAGCGCCAGCCGCCGCACCTGCCGCCAAAGCAGAAAGCAAAG
GCGAGTTTGCCGAGAATGACGCTTACGTGCATGCCACGCCGGTTATCCGTCGTCTGGCGCGTGAGTTCGGTGTGAACCTG
GCGAAGGTGAAAGGGACAGGCCGTAAGGGCCGTATCCTGCGCGAAGACATTCAAGCTTACGTGAAAGATGCCGTGAAACG
TGCCGAAGCTGCACCAGCAGCGGCTGGCGGCGGCCTGCCGGGCATGTTGCCTTGGCCAAAAGTTGATTTCAGTAAATTTG
GTGAAATCGAAGAAGTCGAATTGGGCCGTATCCAGAAAATTTCTGGTGCGAACCTGAGCCGTAACTGGGTCATGATCCCA
CATGTGACGCAATTCGATGAAGCGGATATCACTGAAGTTGAAGCCTTCCGTAAGCAACAGAACATCGAAGCTGAGAAGAA
AAAACAAGACCTGAAAATCACCCCGCTGGTGTTCCTGATGAAGGCCGCCGCTAAAGCACTGGAAGAATTCCCACGCTTTA
ACAGCTCCATTTCCGAAGATGGTCAGAAACTGACGCTGAAGAAATACATCAATATCGGTGTGGCGGTTGATACGCCTAAC
GGCTTGGTAGTTCCAGTATTCCGTGACGTCAACAAAAAGGGTATTGTCGAGTTGTCTCGTGAGCTATCTGTCATCTCCAA
GAAAGCACGTGATGGCAAGCTGACAGCATCTGACATGCAAGGCGGCTGTTTCACTATCTCCAGTCTGGGCGGTATCGGCG
GTACGGCATTTACGCCAATCGTCAATGCGCCAGAAGTGGCTATCTTGGGTGTATCAAAATCATCCATGAAACCTGTCTGG
AATGGTAAAGAGTTTGCTCCACGCCTGATGTTACCGCTGTCTCTGTCCTTCGATCACCGTGTGATTGATGGTGCCGCGGG
TGCACGCTTCGCCGCGTATATCGCTACCATTATGGCGGATATTCGCCGTCTGGTGATGTAA

Upstream 100 bases:

>100_bases
CGCGGTGACATCGACACCAGTGTAGTTGCTGAAGCAATTACTAAGTTTGGTATCGACGCTGATAAAGTTAACCCGCGTCT
GGCATAAGAGGTAGAGAATA

Downstream 100 bases:

>100_bases
TCGCCAAGGCCGGCTTCGTGCCGGCCTTGTTGTGGTTACTGCTCTTGTTATTGGTGATCTTGTTATTACTGATCACCAAT
AGAGAAAAGACACTTATAAA

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNL
AKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIP
HVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPN
GLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVW
NGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM

Sequences:

>Translated_526_residues
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAA
AAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIK
ISTGDKVKTGSLIMVFEVEGAAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNL
AKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIP
HVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPN
GLVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVW
NGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
>Mature_525_residues
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIAVGDKVATGSLIMVFDATGAAA
APVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVTEVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKI
STGDKVKTGSLIMVFEVEGAAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNLA
KVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVELGRIQKISGANLSRNWVMIPH
VTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLMKAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNG
LVVPVFRDVNKKGIVELSRELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVWN
GKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 3 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=420, Percent_Identity=31.6666666666667, Blast_Score=176, Evalue=7e-44,
Organism=Homo sapiens, GI31711992, Length=413, Percent_Identity=31.7191283292978, Blast_Score=158, Evalue=9e-39,
Organism=Homo sapiens, GI19923748, Length=206, Percent_Identity=38.3495145631068, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI203098816, Length=448, Percent_Identity=27.0089285714286, Blast_Score=122, Evalue=1e-27,
Organism=Homo sapiens, GI203098753, Length=436, Percent_Identity=26.8348623853211, Blast_Score=120, Evalue=4e-27,
Organism=Homo sapiens, GI260898739, Length=142, Percent_Identity=37.3239436619718, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=525, Percent_Identity=78.4761904761905, Blast_Score=786, Evalue=0.0,
Organism=Escherichia coli, GI1786946, Length=404, Percent_Identity=31.9306930693069, Blast_Score=178, Evalue=7e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=412, Percent_Identity=29.8543689320388, Blast_Score=178, Evalue=5e-45,
Organism=Caenorhabditis elegans, GI17560088, Length=430, Percent_Identity=28.8372093023256, Blast_Score=134, Evalue=1e-31,
Organism=Caenorhabditis elegans, GI25146366, Length=210, Percent_Identity=38.0952380952381, Blast_Score=129, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=312, Percent_Identity=27.2435897435897, Blast_Score=101, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6320352, Length=429, Percent_Identity=28.4382284382284, Blast_Score=153, Evalue=6e-38,
Organism=Saccharomyces cerevisiae, GI6324258, Length=435, Percent_Identity=27.1264367816092, Blast_Score=119, Evalue=2e-27,
Organism=Drosophila melanogaster, GI18859875, Length=417, Percent_Identity=30.9352517985612, Blast_Score=176, Evalue=3e-44,
Organism=Drosophila melanogaster, GI24645909, Length=214, Percent_Identity=36.4485981308411, Blast_Score=131, Evalue=1e-30,
Organism=Drosophila melanogaster, GI24582497, Length=235, Percent_Identity=28.936170212766, Blast_Score=112, Evalue=5e-25,
Organism=Drosophila melanogaster, GI20129315, Length=235, Percent_Identity=28.936170212766, Blast_Score=111, Evalue=1e-24,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 55452; Mature: 55321

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
CEEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNL
CCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEE
AKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEC
LGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLM
CCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHHHH
KAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVELSR
HHHHHHHHHHHCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHH
ELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVW
HHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCC
NGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
CCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
SIEINVPDIGADEVEVTEIMVKVGDTVEAEQSLITVEGDKASMEVPSPQAGVVKEIKIA
EEEEECCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEE
VGDKVATGSLIMVFDATGAAAAPVKAEEKPAAPAQAAAPAASAAKNVEVPDIGDDEVEVT
ECCEECCCCEEEEEECCCCCCCCCCCCCCCCCCCHHCCCHHHHHCCCCCCCCCCCHHHHH
EVMVKVGDKVDAEQSLITVEGDKASMEVPAPFAGIVKEIKISTGDKVKTGSLIMVFEVEG
HHHHHHCCCCCCCCCEEEEECCCCCEECCCCHHHHHHHHCCCCCCCEECCCEEEEEEECC
AAPAPAAEAAPAQQAAPVAPAPAAAPAAKAESKGEFAENDAYVHATPVIRRLAREFGVNL
CCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHHHCCEE
AKVKGTGRKGRILREDIQAYVKDAVKRAEAAPAAAGGGLPGMLPWPKVDFSKFGEIEEVE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCHHHCCCCCEEC
LGRIQKISGANLSRNWVMIPHVTQFDEADITEVEAFRKQQNIEAEKKKQDLKITPLVFLM
CCCEEEECCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCHHHHHCCCEEHHHHHHH
KAAAKALEEFPRFNSSISEDGQKLTLKKYINIGVAVDTPNGLVVPVFRDVNKKGIVELSR
HHHHHHHHHHHCCCCCHHCCCCEEEEEHEEEEEEEEECCCCEEEEEECCCCCCHHHHHHH
ELSVISKKARDGKLTASDMQGGCFTISSLGGIGGTAFTPIVNAPEVAILGVSKSSMKPVW
HHHHHHHHCCCCCEECCCCCCCEEEEECCCCCCCCHHCCCCCCCCEEEEECCHHCCCCCC
NGKEFAPRLMLPLSLSFDHRVIDGAAGARFAAYIATIMADIRRLVM
CCCCCCCEEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6345153; 9278503; 9298646; 6821375; 2201286; 2121129 [H]