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The map label for this gene is cysG2

Identifier: 153949295

GI number: 153949295

Start: 3714795

End: 3716213

Strand: Reverse

Name: cysG2

Synonym: YpsIP31758_3305

Alternate gene names: 153949295

Gene position: 3716213-3714795 (Counterclockwise)

Preceding gene: 153949424

Following gene: 153947587

Centisome position: 78.68

GC content: 55.67

Gene sequence:

>1419_bases
GTGGACTATCTACCTCTATTTGCCGACTTGAAACAACGCCCGGTATTGATCGTTGGCGGCGGCGAAGTTGCTGCGCGAAA
AATTGAACTACTGCACCGCGCAGGTGCGCAGGTATGGGTAGTGGCGCAAACCCTCTCATCTGAACTCGAACAGCAGTATC
AGGATGGCCGTATCCATTGGCTGGCACAGGATTTTCTGCCTGAACAGTTGGACAACGTGTTTCTGGTGATTGCCGCAACT
AACGATACCGTACTGAATGCCGCTGTTTTCGCGGCGGCAGATCAACGGTGTATTTTGGCGAATGTGGTGGATGACCAACC
CCTTTGTTCGTTTATTTTCCCTTCGATTGTTGATCGTTCACCGCTGGTGGTCGCGATCTCGTCTTCCGGCCAGGCACCGG
TGCTGGCGCGGATACTGCGTGAGAAGTTAGAAGCCTTACTGCCCACTCGTCTCAGTGATATGGCCGCTATAGCTGGGCGC
TGGCGTGGGCGGGTTAAGCAGCATATGGCCTCTATGGGGGAGCGCCGCCGCTTTTGGGAACATGCATTCAGCGGGCGCTT
TGCCAGCCTTATCAGCCGTGGTCAGTTAACCGAGGCTGAAAATGAATTACAACTGTCGCTAGAGGGCCAACACCGTGCAC
TCGGCGAAGTGGCTTTGGTCGGTGCAGGTCCTGGCGATGCAGGGTTATTAACGTTACGTGGTTTGCAAGTGATGCAGCAG
GCAGATGTGGTGCTGTATGACCATTTAGTCAGTCCGGAAGTCTTGGATTTGGTGCGTCGCGATGCTGAACGCATCTGTGT
GGGGAAACGGGCGGGGGCGCATTCGGTTACCCAAGAGGCAACCAATCAGTTGTTGGTCACTCTGGCACAGCAAGGAAAAC
GGGTCGTACGGCTAAAAGGTGGCGATCCCTTTATTTTTGGTCGGGGGGGGGAAGAGTTACAAGTTGTGGCGCAAGCGGGG
ATCCCGTTTCAGGTGGTACCGGGGGTGACGGCTGCTGCGGGGGCGACAGCCTATGCCGGTATTCCCCTGACACACCGCGA
TCATGCGCAAAGCGTGACATTTATTACCGGGCATTGCCGTCCTGATGGCGATGATCTTGATTGGCAGGCGCTGGCCCGTG
GTCGTCAGACGCTGGCGATCTACATGGGAACAGTGAAAGCGGCGGCAATCAGCCAACAGTTGATCGCCCATGGCCGGTCC
AGTACCACGCCGGTGGCAGTGATTGGCCGTGGAACCCGTGTGGACCAGCAGGTGCTGATCGGTACGTTGGCACAACTTGA
ATCACTCGCACAGCAGGCACCGACACCAGCATTACTGGTGATTGGTGAAGTGGTGAATTTACATCACCAAATTGCCTGGT
TTGGGCAACAACCGCAGACTGAATCGGCGATCAACCCATCAGTCGTGAATTTGGCATAA

Upstream 100 bases:

>100_bases
ATATATCATTATGGAACTTGTAATTACTTTTCGTCATTTCATAAGGTCATGGCCCCCCCCTATAGTCACCCTACGCTTTA
TCATGTAATCGAAGGCTGTT

Downstream 100 bases:

>100_bases
AGGATCTGTTATGGACGAAAAACGACTCACTCATTTACGGCAATTGGAGGCGGAGAGTATCCATATCATCCGTGAAGTGG
CCGCTGAATTCGGTAACCCG

Product: siroheme synthase

Products: NA

Alternate protein names: Uroporphyrinogen-III C-methyltransferase; Urogen III methylase; SUMT; Uroporphyrinogen III methylase; UROM; Precorrin-2 dehydrogenase; Sirohydrochlorin ferrochelatase

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAINPSVVNLA

Sequences:

>Translated_472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAINPSVVNLA
>Mature_472_residues
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHWLAQDFLPEQLDNVFLVIAAT
NDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRSPLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGR
WRGRVKQHMASMGERRRFWEHAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKGGDPFIFGRGGEELQVVAQAG
IPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCRPDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRS
STTPVAVIGRGTRVDQQVLIGTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAINPSVVNLA

Specific function: Multifunctional enzyme that catalyzes the SAM-dependent methylation of uroporphyrinogen III at position C-2 and C-7 to form precorrin-2 and then position C-12 or C-18 to form trimethylpyrrocorphin 2. It also catalyzes the conversion of precorrin-2 into si

COG id: COG0007

COG function: function code H; Uroporphyrinogen-III methylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the precorrin methyltransferase family

Homologues:

Organism=Escherichia coli, GI1789768, Length=460, Percent_Identity=56.304347826087, Blast_Score=478, Evalue=1e-136,
Organism=Saccharomyces cerevisiae, GI6322922, Length=255, Percent_Identity=34.5098039215686, Blast_Score=126, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): CYSG1_YERP3 (A7FLY4)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402262.1
- ProteinModelPortal:   A7FLY4
- SMR:   A7FLY4
- STRING:   A7FLY4
- GeneID:   5386338
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3305
- NMPDR:   fig|349747.3.peg.3407
- eggNOG:   COG0007
- HOGENOM:   HBG730212
- OMA:   PVLAQMI
- ProtClustDB:   CLSK889477
- BioCyc:   YPSE349747:YPSIP31758_3305-MONOMER
- GO:   GO:0005488
- HAMAP:   MF_01646
- InterPro:   IPR000878
- InterPro:   IPR014777
- InterPro:   IPR014776
- InterPro:   IPR006366
- InterPro:   IPR016040
- InterPro:   IPR019478
- InterPro:   IPR006367
- InterPro:   IPR003043
- Gene3D:   G3DSA:3.40.1010.10
- Gene3D:   G3DSA:3.30.950.10
- Gene3D:   G3DSA:3.40.50.720
- Gene3D:   G3DSA:1.10.8.210
- TIGRFAMs:   TIGR01469
- TIGRFAMs:   TIGR01470

Pfam domain/function: PF10414 CysG_dimeriser; PF00590 TP_methylase; SSF53790 Cor/por_Metransf

EC number: =2.1.1.107; =1.3.1.76; =4.99.1.4

Molecular weight: Translated: 50902; Mature: 50902

Theoretical pI: Translated: 6.85; Mature: 6.85

Prosite motif: PS00839 SUMT_1; PS00840 SUMT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHW
CCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEE
LAQDFLPEQLDNVFLVIAATNDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRS
EHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
PLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGRWRGRVKQHMASMGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
HHHCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKG
CCCCEEHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGGEELQVVAQAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCR
CCEEEECCCCCEEEEEEECCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
PDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRSSTTPVAVIGRGTRVDQQVLI
CCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAINPSVVNLA
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCEEECC
>Mature Secondary Structure
MDYLPLFADLKQRPVLIVGGGEVAARKIELLHRAGAQVWVVAQTLSSELEQQYQDGRIHW
CCCCCCHHHHCCCCEEEEECCHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCEEE
LAQDFLPEQLDNVFLVIAATNDTVLNAAVFAAADQRCILANVVDDQPLCSFIFPSIVDRS
EHHHHCHHHCCCEEEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHCCCC
PLVVAISSSGQAPVLARILREKLEALLPTRLSDMAAIAGRWRGRVKQHMASMGERRRFWE
CEEEEECCCCCCHHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
HAFSGRFASLISRGQLTEAENELQLSLEGQHRALGEVALVGAGPGDAGLLTLRGLQVMQQ
HHHCCHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHH
ADVVLYDHLVSPEVLDLVRRDAERICVGKRAGAHSVTQEATNQLLVTLAQQGKRVVRLKG
CCCCEEHHCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEC
GDPFIFGRGGEELQVVAQAGIPFQVVPGVTAAAGATAYAGIPLTHRDHAQSVTFITGHCR
CCEEEECCCCCEEEEEEECCCCEEECCCCCHHCCCCCCCCCCCCCCCCCCEEEEEEEECC
PDGDDLDWQALARGRQTLAIYMGTVKAAAISQQLIAHGRSSTTPVAVIGRGTRVDQQVLI
CCCCCCCHHHHHCCCCEEEEEEHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHHH
GTLAQLESLAQQAPTPALLVIGEVVNLHHQIAWFGQQPQTESAINPSVVNLA
HHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHEECCCCCCCCCCCCCCEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA