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The map label for this gene is phoA [H]

Identifier: 153949090

GI number: 153949090

Start: 2122833

End: 2124278

Strand: Direct

Name: phoA [H]

Synonym: YpsIP31758_1835

Alternate gene names: 153949090

Gene position: 2122833-2124278 (Clockwise)

Preceding gene: 153947119

Following gene: 153947858

Centisome position: 44.94

GC content: 50.07

Gene sequence:

>1446_bases
ATGAACCGTGTACCTACTTTATCCGGCATAGCATTATCATCCGGCATAGCATTATCGACTCTGCTACTGTCCAGTGCGCT
ATTAACGCCTAATGCGATTGCGGCTGCTACGGGCTTATATGACCGTGCTGCGCATGGTGATGTGACTCAATTTGGCGGCG
CACGTCGTTTGACTGAAGACCAAACCCAGGCTCTGCGTGACTCGTTATCCAATAAAACAGTAAAAAATGTCATTTTGTTG
ATTGGAGATGGGATGGGTGATTCTGAAATCACGTCTGCCCGTAACTATGCGATGGGGGCCGGTGGTTTCTTTAAAGGCAT
TGATGCGCTGCCGCTGACCGGGCAATACACGCACTATGCACTCGATAAAAAAACACAAAAACCAAATTATGTGACTGATT
CTGCTGCATCAGCGACGGCCTGGTCATCTGGCGTAAAAACGTATAATGGTGCGTTGGGGGTTGATGTTTTTGGTAAAGAC
CATGTTACGTTGCTGGAACTGGCCAAAAAAGCAGGCAAAGCGACGGGTAATGTCTCTACCGCTGAACTACAAGATGCCAC
TCCTGCTGCGCAGTTTGCCCACGTGACCGGGCGTAAGTGCTATGGCCCGGAAGAAACCAGTGAGAAATGCAGTACCAATG
CATTGGAAAAAGGGGGCCGTGGCTCTATTACTGAGCAGATGATTGAAGGGCGCGCTGATGTCACGCTTGGCGGTGGTGCA
GCATCATTCAGCCAGGTCGCTAAAGCCGGTGAGTGGAAAGACCAGTCGTTGCGTGATCAGGCGTTGGCCCGCGGCTACGT
CATTGTTGAGAACCTTGATGACCTGAATGCAATAAAACACGCTAATCAACAGAAACCTCTATTGGGTTTATTCTCGCCAG
GTAATATGCCAGTGCGTTGGCAGGGGCCAAAAGCCTCTTATCATGGCAATCTGGATAAACCGCCAGTGGTGTGTGAAAAC
AATGCTGAGCGGACCAGCGACATTCCAACGTTGGCTGTCATGACAGAAAAAGCCATTGACCTGCTGAAAACCAATGAGAA
TGGCTTCTTCTTGCAAGTTGAAGGGGCGTCGATTGATAAACAGGATCACTCCGCTAACCCATGTGGTCAGTTTGGTGAAA
CGGTTGATCTGGATGAAGCGGTACAAAAAGCGCTGGAATTCGCCCGTGCTGATGGCAACACTTTGGTGATTGTGACGGCT
GACCATGCTCACTCCAGCCAAATTATCGAGCCTGAAGCGAAGGCTCCGGGCCTGACTCAGGCATTAACGACCAAAGATGG
CGCGGTCATGGCGATCAGCTACGGTAACTCTGAAGACGATTCTCAAGGCCATACTGGGTCACAATTGCGGATAGCGGCTT
ATGGTCCTCACGCGGCTAACGTCGTAGGTTTAACTGACCAAACTGATTTGTTCTTCACCATGCGGGATGCGATGGCACTT
AAATAA

Upstream 100 bases:

>100_bases
TAGTGGTATCTGTCATAAAAGCGTCACCCGGCTGTTCTAGTATCCGTTGTAACCAATCAATACAAATTGTATGACTGTTT
TACAACAAGGAATTATATCG

Downstream 100 bases:

>100_bases
GTCGCCTGTTATCTCACTGGAACGTCCCGGCGCAGTAATGGCCGGGATTTTTGCTTATGTCCCGTCACGGTAATCCTGCT
TATATCTGGAAAATTCTGTT

Product: alkaline phosphatase

Products: NA

Alternate protein names: APase [H]

Number of amino acids: Translated: 481; Mature: 481

Protein sequence:

>481_residues
MNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLSNKTVKNVILL
IGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKD
HVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGRADVTLGGGA
ASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGNLDKPPVVCEN
NAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTA
DHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLFFTMRDAMAL
K

Sequences:

>Translated_481_residues
MNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLSNKTVKNVILL
IGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKD
HVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGRADVTLGGGA
ASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGNLDKPPVVCEN
NAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTA
DHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLFFTMRDAMAL
K
>Mature_481_residues
MNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTEDQTQALRDSLSNKTVKNVILL
IGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYALDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKD
HVTLLELAKKAGKATGNVSTAELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGRADVTLGGGA
ASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRWQGPKASYHGNLDKPPVVCEN
NAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDKQDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTA
DHAHSSQIIEPEAKAPGLTQALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLFFTMRDAMAL
K

Specific function: Unknown

COG id: COG1785

COG function: function code P; Alkaline phosphatase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the alkaline phosphatase family [H]

Homologues:

Organism=Homo sapiens, GI94721246, Length=497, Percent_Identity=27.9678068410463, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI157266296, Length=496, Percent_Identity=28.0241935483871, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI116734717, Length=407, Percent_Identity=29.97542997543, Blast_Score=118, Evalue=1e-26,
Organism=Homo sapiens, GI157266292, Length=357, Percent_Identity=28.8515406162465, Blast_Score=111, Evalue=2e-24,
Organism=Homo sapiens, GI294660770, Length=397, Percent_Identity=29.4710327455919, Blast_Score=109, Evalue=7e-24,
Organism=Homo sapiens, GI294660772, Length=345, Percent_Identity=29.8550724637681, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI48994877, Length=468, Percent_Identity=74.1452991452991, Blast_Score=714, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320689, Length=361, Percent_Identity=32.409972299169, Blast_Score=122, Evalue=1e-28,
Organism=Drosophila melanogaster, GI21355151, Length=422, Percent_Identity=30.8056872037915, Blast_Score=143, Evalue=2e-34,
Organism=Drosophila melanogaster, GI19921912, Length=380, Percent_Identity=30.2631578947368, Blast_Score=124, Evalue=1e-28,
Organism=Drosophila melanogaster, GI18859923, Length=361, Percent_Identity=31.0249307479224, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24651554, Length=405, Percent_Identity=29.3827160493827, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21355981, Length=363, Percent_Identity=30.5785123966942, Blast_Score=120, Evalue=2e-27,
Organism=Drosophila melanogaster, GI21355149, Length=435, Percent_Identity=28.0459770114943, Blast_Score=120, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24585213, Length=385, Percent_Identity=29.6103896103896, Blast_Score=116, Evalue=4e-26,
Organism=Drosophila melanogaster, GI85725200, Length=381, Percent_Identity=28.6089238845144, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI85815833, Length=381, Percent_Identity=28.6089238845144, Blast_Score=108, Evalue=6e-24,
Organism=Drosophila melanogaster, GI21358067, Length=393, Percent_Identity=28.2442748091603, Blast_Score=103, Evalue=4e-22,
Organism=Drosophila melanogaster, GI24651556, Length=371, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24657827, Length=393, Percent_Identity=29.7709923664122, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24657835, Length=397, Percent_Identity=27.2040302267003, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24657842, Length=382, Percent_Identity=26.7015706806283, Blast_Score=79, Evalue=8e-15,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017849
- InterPro:   IPR001952
- InterPro:   IPR018299
- InterPro:   IPR017850 [H]

Pfam domain/function: PF00245 Alk_phosphatase [H]

EC number: =3.1.3.1 [H]

Molecular weight: Translated: 50743; Mature: 50743

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: PS00123 ALKALINE_PHOSPHATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTED
CCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
QTQALRDSLSNKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYA
HHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEE
LDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKDHVTLLELAKKAGKATGNVST
ECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCH
AELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGRADVTLGGGA
HHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCH
ASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRW
HHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCCCEEE
QGPKASYHGNLDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDK
CCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHEECCCCEEEEEECCCCCC
QDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTADHAHSSQIIEPEAKAPGLTQ
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
ALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLFFTMRDAMAL
HHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCEEEEECHHHCC
K
C
>Mature Secondary Structure
MNRVPTLSGIALSSGIALSTLLLSSALLTPNAIAAATGLYDRAAHGDVTQFGGARRLTED
CCCCCCCCCHHHHCCHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCHHHCCCCCCCCHH
QTQALRDSLSNKTVKNVILLIGDGMGDSEITSARNYAMGAGGFFKGIDALPLTGQYTHYA
HHHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCHHCCCCCCCCCCCCEEEE
LDKKTQKPNYVTDSAASATAWSSGVKTYNGALGVDVFGKDHVTLLELAKKAGKATGNVST
ECCCCCCCCCCCCCCCCHHHHHCCCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCCCCH
AELQDATPAAQFAHVTGRKCYGPEETSEKCSTNALEKGGRGSITEQMIEGRADVTLGGGA
HHHCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHCCCCCEEECCCH
ASFSQVAKAGEWKDQSLRDQALARGYVIVENLDDLNAIKHANQQKPLLGLFSPGNMPVRW
HHHHHHHHCCCCCCHHHHHHHHHCCEEEEECCCHHHHHHHCCCCCCEEEEECCCCCCEEE
QGPKASYHGNLDKPPVVCENNAERTSDIPTLAVMTEKAIDLLKTNENGFFLQVEGASIDK
CCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEHHHHHHHHHHEECCCCEEEEEECCCCCC
QDHSANPCGQFGETVDLDEAVQKALEFARADGNTLVIVTADHAHSSQIIEPEAKAPGLTQ
CCCCCCCHHHCCCCCCHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCCCCCCCCCHH
ALTTKDGAVMAISYGNSEDDSQGHTGSQLRIAAYGPHAANVVGLTDQTDLFFTMRDAMAL
HHCCCCCEEEEEECCCCCCCCCCCCCCEEEEEEECCCCCEEEECCCCCCEEEEECHHHCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2129542 [H]