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The map label for this gene is glyS [H]

Identifier: 153949017

GI number: 153949017

Start: 4662982

End: 4665051

Strand: Reverse

Name: glyS [H]

Synonym: YpsIP31758_4130

Alternate gene names: 153949017

Gene position: 4665051-4662982 (Counterclockwise)

Preceding gene: 153949369

Following gene: 153948705

Centisome position: 98.77

GC content: 52.22

Gene sequence:

>2070_bases
ATGACTCAACAGACTTTCCTGGTGGAAATCGGCACGGAAGAGCTGCCGCCGAAGGCTCTTCGTTCTCTGGCCGAATCTTT
TGCCGCTAATTTTACGGCTGAACTGGATAACGCTAATTTGTCTCACGGTGAGGTGAGTTGGTACGCCGCGCCGCGCCGTC
TGGCAGTAAAAGTGGCCAATTTAAGTGCGGCTCAGGCTGATCGTGAAGTCGAAAAGCGCGGCCCGGCCATTGCGCAAGCG
TTTGATGCTGAAGGTAAACCTAGCAAAGCCGCTGAAGGTTGGGCGCGTGGTTGTGGTATTACAGTCGATCAGGCGGAGCG
TCTGGTGACGGATAAAGGCGAGTGGCTGCTGTACCGTGCCCATGTCAAAGGGCAACCCGCCCAATTATTGCTGGCAGGCA
TGGTTAATACCGCGCTGAGTAAGTTGCCAATTCCAAAGCTGATGCGTTGGGGTGATAAAGAAACTCAATTCGTCCGTCCG
GTGCATACCGTGACCCTGCTCTTGGGGACTGAAGTTATTCCTGGCACCGTGCTGGGTATCAATTCAGATCGGGTGATCCG
TGGCCATCGCTTTATGGGTGAAGCGGAATTCACGATCGACAGTGCTGACCAATATCCACAGATTCTGCTGGAGCGTGGCA
AGGTCATTGCTGATTATGAATTGCGTAAATCAATCATTAAGCGTGATGCAGAACAGGCAGCGCAACAGATTGGCGGTGTT
GCTGATCTGAGTGAAAGCCTGCTAGAGGAAGTGGCGTCATTGGTGGAGTGGCCGGTGGTGCTCACCGCCAAGTTTGAAGA
GAAATTCCTGGCTGTCCCTGCGGAAGCGCTGGTGTACACCATGAAGGGTGATCAGAAGTATTTCCCGGTTTACGATACTG
CCGGCCACTTGATGCCACACTTCATTTTTGTCGCCAACATTGAGTCTAAAGACCCTCAACAAATCATTTCTGGCAATGAG
AAGGTGGTTCGCCCTCGTTTGGCTGATGCTGAGTTCTTCTTTAAGACCGACCGCAAAAAACGGTTGGAAGATAATTTACC
GCGTCTGGAAACCGTGCTGTTCCAACAGCAACTGGGTACCCTGCGTGATAAAACAGATCGTATTCAGGCGTTGGCGGGTT
GGGTTGCCGCACAAATTGGCGCTGATGTTAATCACGCCACCCGTGCGGGTCTGTTGTCCAAGTGTGACCTGATGACCAAC
ATGGTATTCGAATTCACCGATACCCAAGGCGTGATGGGGATGCACTACGCCCGCCACGATGGTGAGGCTGAAGACGTTGC
CGTTGCATTGAATGAACAATATCAGCCACGCTTTGCCGGTGATGATTTGCCGTCTAACCCGGTCGCTTGTGCGCTGGCCA
TTGCCGATAAGATGGACACACTGGCGGGTATCTTCGGTATCGGTCAACATCCGAAAGGCGATAAAGATCCGTTTGCTCTG
CGCCGTGCGGCGTTGGGTGTACTGCGCATTATCGTCGAAAAGAATTTGTCACTGGATCTGCAAACACTGACGGAAGAGGC
GGTGCGCCTGTACGGCAGTAAGCTGACCAATGCGAAAGTGGTCGATGACGTGATTGAATTCATGCTGGGCCGTTTCCGTG
CCTGGTATCAGGATGAAGGTCATAGCGTCGATACCATTCAGGCGGTATTGGCTCGTCGTCCGACCAAACCGGCTGATTTT
GATGCGCGAGTGAAAGCGGTGACCTATTTCCGCACACTGGATGCTGCTGCCGCATTGGCTGCTGCCAACAAACGTGTATC
AAACATTCTGGCAAAATCCACGGATACCCTGAATGACCATGTTCATGCTTCGATCTTAAAAGAACCGGCAGAGCTAAAAC
TGGCGACACATTTGGTGGTATTACGCGATCAACTGGAGCCGGTTTTCGCGGCTGGTCAGTATAAAGAGGCGCTGGTTGAA
TTAGCTGCGTTGCGGGAAACTGTCGATGAGTTCTTCGAGAGCGTGATGGTGATGGCTGAAGACGATGCTGTACGGGTTAA
CCGTCTGACATTGCTGAGCAAACTCCGCGAACTGTTCTTGCAGGTTGCTGATATTTCGCTGTTGCAATAA

Upstream 100 bases:

>100_bases
CATCCGTACGCTGACCAAAGCTGTCGCCGAGGCATATTATGCCTCCCGCGAGGCATTGGGCTTCCCTATGTGCAAAAAGA
ATCAGAACTAAGAGGCTGTC

Downstream 100 bases:

>100_bases
CTCGCTCGATAACATCGTGCTGGGCAATAAGGGGAGCTATACGCTCCCCTTCAGACTGCTGACAAACCCCGATGACGCGA
TCTTGAACGGTGAGGATAGG

Product: glycyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Glycine--tRNA ligase beta subunit; GlyRS [H]

Number of amino acids: Translated: 689; Mature: 688

Protein sequence:

>689_residues
MTQQTFLVEIGTEELPPKALRSLAESFAANFTAELDNANLSHGEVSWYAAPRRLAVKVANLSAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQPAQLLLAGMVNTALSKLPIPKLMRWGDKETQFVRP
VHTVTLLLGTEVIPGTVLGINSDRVIRGHRFMGEAEFTIDSADQYPQILLERGKVIADYELRKSIIKRDAEQAAQQIGGV
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDTAGHLMPHFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFKTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAAQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLSLDLQTLTEEAVRLYGSKLTNAKVVDDVIEFMLGRFRAWYQDEGHSVDTIQAVLARRPTKPADF
DARVKAVTYFRTLDAAAALAAANKRVSNILAKSTDTLNDHVHASILKEPAELKLATHLVVLRDQLEPVFAAGQYKEALVE
LAALRETVDEFFESVMVMAEDDAVRVNRLTLLSKLRELFLQVADISLLQ

Sequences:

>Translated_689_residues
MTQQTFLVEIGTEELPPKALRSLAESFAANFTAELDNANLSHGEVSWYAAPRRLAVKVANLSAAQADREVEKRGPAIAQA
FDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQPAQLLLAGMVNTALSKLPIPKLMRWGDKETQFVRP
VHTVTLLLGTEVIPGTVLGINSDRVIRGHRFMGEAEFTIDSADQYPQILLERGKVIADYELRKSIIKRDAEQAAQQIGGV
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDTAGHLMPHFIFVANIESKDPQQIISGNE
KVVRPRLADAEFFFKTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAAQIGADVNHATRAGLLSKCDLMTN
MVFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
RRAALGVLRIIVEKNLSLDLQTLTEEAVRLYGSKLTNAKVVDDVIEFMLGRFRAWYQDEGHSVDTIQAVLARRPTKPADF
DARVKAVTYFRTLDAAAALAAANKRVSNILAKSTDTLNDHVHASILKEPAELKLATHLVVLRDQLEPVFAAGQYKEALVE
LAALRETVDEFFESVMVMAEDDAVRVNRLTLLSKLRELFLQVADISLLQ
>Mature_688_residues
TQQTFLVEIGTEELPPKALRSLAESFAANFTAELDNANLSHGEVSWYAAPRRLAVKVANLSAAQADREVEKRGPAIAQAF
DAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRAHVKGQPAQLLLAGMVNTALSKLPIPKLMRWGDKETQFVRPV
HTVTLLLGTEVIPGTVLGINSDRVIRGHRFMGEAEFTIDSADQYPQILLERGKVIADYELRKSIIKRDAEQAAQQIGGVA
DLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDTAGHLMPHFIFVANIESKDPQQIISGNEK
VVRPRLADAEFFFKTDRKKRLEDNLPRLETVLFQQQLGTLRDKTDRIQALAGWVAAQIGADVNHATRAGLLSKCDLMTNM
VFEFTDTQGVMGMHYARHDGEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFALR
RAALGVLRIIVEKNLSLDLQTLTEEAVRLYGSKLTNAKVVDDVIEFMLGRFRAWYQDEGHSVDTIQAVLARRPTKPADFD
ARVKAVTYFRTLDAAAALAAANKRVSNILAKSTDTLNDHVHASILKEPAELKLATHLVVLRDQLEPVFAAGQYKEALVEL
AALRETVDEFFESVMVMAEDDAVRVNRLTLLSKLRELFLQVADISLLQ

Specific function: Unknown

COG id: COG0751

COG function: function code J; Glycyl-tRNA synthetase, beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Escherichia coli, GI1789982, Length=689, Percent_Identity=85.6313497822932, Blast_Score=1205, Evalue=0.0,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 400 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008909
- InterPro:   IPR006194
- InterPro:   IPR015944
- InterPro:   IPR002311 [H]

Pfam domain/function: PF05746 DALR_1; PF02092 tRNA_synt_2f [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 76205; Mature: 76074

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: PS50861 AA_TRNA_LIGASE_II_GLYAB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQQTFLVEIGTEELPPKALRSLAESFAANFTAELDNANLSHGEVSWYAAPRRLAVKVAN
CCCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCEEEEEC
LSAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRA
CHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
HVKGQPAQLLLAGMVNTALSKLPIPKLMRWGDKETQFVRPVHTVTLLLGTEVIPGTVLGI
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEC
NSDRVIRGHRFMGEAEFTIDSADQYPQILLERGKVIADYELRKSIIKRDAEQAAQQIGGV
CCCCEEECCCCCCCCEEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHCCH
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDTAGHLMPH
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHHHH
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFKTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWVAAQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLSLDLQTLTEEAVRLYGSKLTNAKVVDDVIEFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
HSVDTIQAVLARRPTKPADFDARVKAVTYFRTLDAAAALAAANKRVSNILAKSTDTLNDH
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VHASILKEPAELKLATHLVVLRDQLEPVFAAGQYKEALVELAALRETVDEFFESVMVMAE
HHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DDAVRVNRLTLLSKLRELFLQVADISLLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
TQQTFLVEIGTEELPPKALRSLAESFAANFTAELDNANLSHGEVSWYAAPRRLAVKVAN
CCCEEEEEECCCCCCHHHHHHHHHHHHHCCEEECCCCCCCCCCEEEEECCCCCEEEEEC
LSAAQADREVEKRGPAIAQAFDAEGKPSKAAEGWARGCGITVDQAERLVTDKGEWLLYRA
CHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHCCCCCHHHHHHHHCCCCCEEEEEE
HVKGQPAQLLLAGMVNTALSKLPIPKLMRWGDKETQFVRPVHTVTLLLGTEVIPGTVLGI
CCCCCHHHHHHHHHHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEC
NSDRVIRGHRFMGEAEFTIDSADQYPQILLERGKVIADYELRKSIIKRDAEQAAQQIGGV
CCCCEEECCCCCCCCEEEECCCHHHHHHHHHCCCEEECHHHHHHHHHHHHHHHHHHHCCH
ADLSESLLEEVASLVEWPVVLTAKFEEKFLAVPAEALVYTMKGDQKYFPVYDTAGHLMPH
HHHHHHHHHHHHHHHHCCEEEEEEHHHHHHCCCHHHEEEEECCCCEECCCCCCCHHHHHH
FIFVANIESKDPQQIISGNEKVVRPRLADAEFFFKTDRKKRLEDNLPRLETVLFQQQLGT
EEEEEECCCCCHHHHHCCCCCEECCCCCCCHHHHHCCHHHHHHCCCHHHHHHHHHHHHHH
LRDKTDRIQALAGWVAAQIGADVNHATRAGLLSKCDLMTNMVFEFTDTQGVMGMHYARHD
HHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCCCCCHHHHHHCCC
GEAEDVAVALNEQYQPRFAGDDLPSNPVACALAIADKMDTLAGIFGIGQHPKGDKDPFAL
CCHHHEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
RRAALGVLRIIVEKNLSLDLQTLTEEAVRLYGSKLTNAKVVDDVIEFMLGRFRAWYQDEG
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCC
HSVDTIQAVLARRPTKPADFDARVKAVTYFRTLDAAAALAAANKRVSNILAKSTDTLNDH
CCHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHH
VHASILKEPAELKLATHLVVLRDQLEPVFAAGQYKEALVELAALRETVDEFFESVMVMAE
HHHHHHCCCHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEEC
DDAVRVNRLTLLSKLRELFLQVADISLLQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA