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The map label for this gene is thiE

Identifier: 153948968

GI number: 153948968

Start: 4341077

End: 4341724

Strand: Direct

Name: thiE

Synonym: YpsIP31758_3854

Alternate gene names: 153948968

Gene position: 4341077-4341724 (Clockwise)

Preceding gene: 153949362

Following gene: 153948298

Centisome position: 91.91

GC content: 53.24

Gene sequence:

>648_bases
GTGGCTACACCAGGTTTCCCCTCCACGGAACAGCGTCTTGGGCTCTACCCAGTGGTGGACTCATTATTATGGATAGAACG
CCTGCTGGCAGCCGGAGTCACGACGCTACAACTGCGCATTAAAAATGCAGACGATGCTCAGGTGGAGCAAGATATTGTCG
CGGCCATTGAATTAGGAAAACGCTATCAGGCGCGCTTATTTATCAATGATTACTGGCAGTTGGCGGTTAAACACGGCGCT
TATGGCGTTCATCTCGGTCAGGAAGATCTGGAAACCGCTGATTTAGCGGCAATTCAGCAAGCGGGGTTACGGCTCGGCAT
TTCTACCCATGATGAACATGAGTTGGCGGTGGCAAAAACACTGCGCCCCTCTTATATCGCGCTGGGGCATATTTTCCCTA
CTCAGACCAAACAGATGCCGTCATCCCCACAGGGGTTGGCGTCTTTAAGCCGTCAGGTGAAGAATACGCCGGATTACCCA
ACCGTGGCTATCGGTGGCATCAGTATTGAGCGGGTGCCACACGTATTAGCAACTGGCGTCGGCAGTGTCGCCGTGGTCAG
CGCCATTACGTTGGCAAGTGACTGGCAACGGGCAACCGCACAATTACTGCACCTGATCGAGGGTAAGGAGCTGGCTGATG
AAAAATGA

Upstream 100 bases:

>100_bases
ATGTCAGCCGAGTTTCGCTCCCGTGGCAGTGAGCTATACCACCGCCCGGCGAATCTGAGTGCGGAGGCCAATAATGAGCC
AACTTGATGCCCTCTCTCCT

Downstream 100 bases:

>100_bases
CCAGACGCTAAGTGACAGTGAATTTTTACGCTATAGCCGCCAACTGCTACTGGAAGATATCGGCCCGGAAGGGCAACGAA
AGCTAAAGGGTGCCAGTGTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGA
YGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYP
TVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Sequences:

>Translated_215_residues
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGA
YGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYP
TVAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
>Mature_214_residues
ATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGKRYQARLFINDYWQLAVKHGAY
GVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKTLRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPT
VAIGGISIERVPHVLATGVGSVAVVSAITLASDWQRATAQLLHLIEGKELADEK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Escherichia coli, GI1790426, Length=205, Percent_Identity=70.2439024390244, Blast_Score=294, Evalue=4e-81,
Organism=Saccharomyces cerevisiae, GI6325042, Length=228, Percent_Identity=28.0701754385965, Blast_Score=69, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_YERP3 (A7FNH7)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001402805.1
- ProteinModelPortal:   A7FNH7
- STRING:   A7FNH7
- GeneID:   5386729
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_3854
- eggNOG:   COG0352
- HOGENOM:   HBG754477
- OMA:   HAVQPSY
- ProtClustDB:   PRK03512
- BioCyc:   YPSE349747:YPSIP31758_3854-MONOMER
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 23305; Mature: 23174

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
0.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGK
CCCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
RYQARLFINDYWQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKT
HHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
LRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIERVPHVLATGV
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
GSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC
>Mature Secondary Structure 
ATPGFPSTEQRLGLYPVVDSLLWIERLLAAGVTTLQLRIKNADDAQVEQDIVAAIELGK
CCCCCCCHHHHCCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHCC
RYQARLFINDYWQLAVKHGAYGVHLGQEDLETADLAAIQQAGLRLGISTHDEHELAVAKT
HHEEEEEEHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHCCCEECCCCCCCHHHHHHHH
LRPSYIALGHIFPTQTKQMPSSPQGLASLSRQVKNTPDYPTVAIGGISIERVPHVLATGV
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEECCCCHHHHHHHHHHCC
GSVAVVSAITLASDWQRATAQLLHLIEGKELADEK
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA