Click here to switch to the map view.

The map label for this gene is nuoB

Identifier: 153948912

GI number: 153948912

Start: 1693859

End: 1694542

Strand: Direct

Name: nuoB

Synonym: YpsIP31758_1455

Alternate gene names: 153948912

Gene position: 1693859-1694542 (Clockwise)

Preceding gene: 153947088

Following gene: 153949467

Centisome position: 35.86

GC content: 49.71

Gene sequence:

>684_bases
ATGGACTATACGCTCACCCGCATAGACCCTAACGGTGAGAATGACCGTTACCCCCTGCAAACTCAGGAAACCGTCAGCGG
CGATCCTCTCGAGCAACATGTCCACCGTAGTGTTTACATGGGTAAACTTGAAAACGCCATGCATGACATGGTGAACTGGG
GGCGTAAAAACTCTCTTTGGCCTTATAACTTTGGCCTTTCTTGTTGTTATGTCGAGATGGTGACTTCGTTCACGGCGGTA
CATGACGTCGCCCGTTTCGGTGCTGAAGTCTTGCGTGCATCCCCACGTCAGGCCGATTTTATGGTGGTGGCCGGAACCTG
CTTTACCAAAATGGCCCCGGTTATTCAACGTTTATATGAACAAATGCTGGAACCAAAATGGGTTATTTCCATGGGGGCCT
GCGCGAATTCTGGTGGCATGTACGATATCTACTCCGTTGTGCAAGGTGTGGATAAATTCCTACCAGTAGATGTCTATATT
CCTGGCTGTCCACCACGCCCGGAGGCGTATATGCAAGCGCTATTGCTATTGCTATTGCAGGAATCCATTGGTAAAGAGCG
TCGTCCTCTCTCTTGGGTTGTCGGTGACCAAGGTGTCTACCGTGCCAATATGCAGCCAGAAAGAGAACGTAAGCATGCTG
AACGAATTGCGGTAACTAACCTGCGTACACCTGACGAGATTTAA

Upstream 100 bases:

>100_bases
CTGACATCAGTCAGTGATTCGGGTCAGTGAACCTAGCTAACAACCCAGCAGCGCCAAGTGCAAAAGGTATAGTGGTCAAT
AAATATAGCGAGGCATTAAC

Downstream 100 bases:

>100_bases
ACCCGTTTTTTGGGCGGTAGATCCCGGTGCGTAAGTGATGTGTTTTTGGAGAGAACGTAATTTTCTTCTGGGGCATATAT
GCAGCAAATGACCACAAAAT

Product: NADH dehydrogenase subunit B

Products: NA

Alternate protein names: NADH dehydrogenase I subunit B; NDH-1 subunit B

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI

Sequences:

>Translated_227_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
>Mature_227_residues
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLWPYNFGLSCCYVEMVTSFTAV
HDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYEQMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYI
PGCPPRPEAYMQALLLLLLQESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG0377

COG function: function code C; NADH:ubiquinone oxidoreductase 20 kD subunit and related Fe-S oxidoreductases

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 20 kDa subunit family

Homologues:

Organism=Homo sapiens, GI187281616, Length=147, Percent_Identity=53.7414965986395, Blast_Score=165, Evalue=2e-41,
Organism=Escherichia coli, GI1788624, Length=227, Percent_Identity=89.4273127753304, Blast_Score=419, Evalue=1e-119,
Organism=Escherichia coli, GI1789074, Length=122, Percent_Identity=42.6229508196721, Blast_Score=97, Evalue=7e-22,
Organism=Escherichia coli, GI1788834, Length=160, Percent_Identity=35.625, Blast_Score=95, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17509685, Length=147, Percent_Identity=49.6598639455782, Blast_Score=153, Evalue=6e-38,
Organism=Drosophila melanogaster, GI18859983, Length=132, Percent_Identity=56.0606060606061, Blast_Score=163, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24642371, Length=132, Percent_Identity=56.0606060606061, Blast_Score=163, Evalue=7e-41,
Organism=Drosophila melanogaster, GI24651058, Length=147, Percent_Identity=53.0612244897959, Blast_Score=162, Evalue=2e-40,

Paralogues:

None

Copy number: 520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NUOB_YERP3 (A7FGQ6)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001400434.1
- STRING:   A7FGQ6
- GeneID:   5387974
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1455
- NMPDR:   fig|349747.3.peg.1077
- eggNOG:   COG0377
- HOGENOM:   HBG553221
- OMA:   PRPEAYI
- ProtClustDB:   PRK06411
- BioCyc:   YPSE349747:YPSIP31758_1455-MONOMER
- GO:   GO:0006810
- HAMAP:   MF_01356
- InterPro:   IPR006137
- InterPro:   IPR006138
- InterPro:   IPR014406
- PANTHER:   PTHR11995:SF2
- PANTHER:   PTHR11995
- TIGRFAMs:   TIGR01957

Pfam domain/function: PF01058 Oxidored_q6

EC number: =1.6.99.5

Molecular weight: Translated: 25838; Mature: 25838

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS01150 COMPLEX1_20K

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
5.3 %Met     (Mature Protein)
7.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLW
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALLLLLLQ
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
ESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
HHHCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MDYTLTRIDPNGENDRYPLQTQETVSGDPLEQHVHRSVYMGKLENAMHDMVNWGRKNSLW
CCCEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PYNFGLSCCYVEMVTSFTAVHDVARFGAEVLRASPRQADFMVVAGTCFTKMAPVIQRLYE
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCHHHHHHHHHHHHHHH
QMLEPKWVISMGACANSGGMYDIYSVVQGVDKFLPVDVYIPGCPPRPEAYMQALLLLLLQ
HHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEECCCCCCCHHHHHHHHHHHHH
ESIGKERRPLSWVVGDQGVYRANMQPERERKHAERIAVTNLRTPDEI
HHHCCCCCCCEEEECCCCEEECCCCCHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA