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The map label for this gene is trmB

Identifier: 153948853

GI number: 153948853

Start: 991679

End: 992398

Strand: Direct

Name: trmB

Synonym: YpsIP31758_0820

Alternate gene names: 153948853

Gene position: 991679-992398 (Clockwise)

Preceding gene: 153948240

Following gene: 153948134

Centisome position: 21.0

GC content: 51.67

Gene sequence:

>720_bases
ATGATAAATGACGTCATATCTCCGGAATTTGATGAAAACGGCCGAGCACTGCGCCGTATTCGTAGTTTTGTCCGCCGTCA
GGGGCGCCTGACCAAGGGGCAGCAACTGGCTCTGGACAGTTACTGGCCGGTGATGGGCGTGGAATATCAGGCGGCCCCGG
TTGACTTGAATACCTTGTTTGGCCGTGAGGCCCCGATAGTACTGGAAATCGGTTTTGGTATGGGGACCTCACTGGTGACC
ATGGCTGCCAATAATCCGCAGCAAAACTTTTTAGGCATTGAGGTGCATTCACCGGGGGTCGGTGCTTGCCTGAGTTCAGC
ACATGACGCCGGCCTGAGTAACCTGCGGATCATGTGTCATGATGCGGTCGAAGTTCTGGAGAATATGATCCCAGAGGCTT
CATTGGACATGGTACAACTCTTTTTCCCCGACCCATGGCACAAAGCGCGCCATAATAAGCGTCGTATCGTTCAAACACCG
TTTGTCGAGCTGGTAAAAAGTAAATTAAAGGTGGGGGGCGTATTTCACATGGCGACCGACTGGCAACCTTATGCGGAACA
TATGCTGGAGGTCATGTCGGGTGTGAGCGGTTATCTCAACCTTTCAGAGCAAAATGATTACGTACCCCGTCCGGACTCAC
GTCCACTGACAAAATTCGAATTACGTGGCCAGCGTCTGGGACATGGCGTTTGGGATTTGATGTTTGAGAGGAAAGAATAA

Upstream 100 bases:

>100_bases
TTACCGCTTAATTTTGTACTTATGACGTTCATAGTTGATAAACTGGCGCATCTTTGCATAATGCGCGTTCATATGCACAA
CCAACAGATAGAAAGCACTA

Downstream 100 bases:

>100_bases
TGGCTAAGAATCGCAGTCGTCGTTTACGCAAAAAGATGCATATCGACGAGTTTCAAGAATTGGGTTTTTCCGTACAGTGG
GCTTTTGATGAAGGCACTAG

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase

Number of amino acids: Translated: 239; Mature: 239

Protein sequence:

>239_residues
MINDVISPEFDENGRALRRIRSFVRRQGRLTKGQQLALDSYWPVMGVEYQAAPVDLNTLFGREAPIVLEIGFGMGTSLVT
MAANNPQQNFLGIEVHSPGVGACLSSAHDAGLSNLRIMCHDAVEVLENMIPEASLDMVQLFFPDPWHKARHNKRRIVQTP
FVELVKSKLKVGGVFHMATDWQPYAEHMLEVMSGVSGYLNLSEQNDYVPRPDSRPLTKFELRGQRLGHGVWDLMFERKE

Sequences:

>Translated_239_residues
MINDVISPEFDENGRALRRIRSFVRRQGRLTKGQQLALDSYWPVMGVEYQAAPVDLNTLFGREAPIVLEIGFGMGTSLVT
MAANNPQQNFLGIEVHSPGVGACLSSAHDAGLSNLRIMCHDAVEVLENMIPEASLDMVQLFFPDPWHKARHNKRRIVQTP
FVELVKSKLKVGGVFHMATDWQPYAEHMLEVMSGVSGYLNLSEQNDYVPRPDSRPLTKFELRGQRLGHGVWDLMFERKE
>Mature_239_residues
MINDVISPEFDENGRALRRIRSFVRRQGRLTKGQQLALDSYWPVMGVEYQAAPVDLNTLFGREAPIVLEIGFGMGTSLVT
MAANNPQQNFLGIEVHSPGVGACLSSAHDAGLSNLRIMCHDAVEVLENMIPEASLDMVQLFFPDPWHKARHNKRRIVQTP
FVELVKSKLKVGGVFHMATDWQPYAEHMLEVMSGVSGYLNLSEQNDYVPRPDSRPLTKFELRGQRLGHGVWDLMFERKE

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family

Homologues:

Organism=Escherichia coli, GI1789330, Length=237, Percent_Identity=80.168776371308, Blast_Score=407, Evalue=1e-115,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMB_YERP3 (A7FEX6)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399804.1
- ProteinModelPortal:   A7FEX6
- SMR:   A7FEX6
- STRING:   A7FEX6
- GeneID:   5388293
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_0820
- NMPDR:   fig|349747.3.peg.1721
- eggNOG:   COG0220
- HOGENOM:   HBG322782
- OMA:   TDWEEYA
- ProtClustDB:   PRK00121
- BioCyc:   YPSE349747:YPSIP31758_0820-MONOMER
- HAMAP:   MF_01057
- InterPro:   IPR003358
- PANTHER:   PTHR23417:SF1
- TIGRFAMs:   TIGR00091

Pfam domain/function: PF02390 Methyltransf_4

EC number: =2.1.1.33

Molecular weight: Translated: 26989; Mature: 26989

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: NA

Important sites: BINDING 69-69 BINDING 94-94 BINDING 121-121 BINDING 144-144 BINDING 148-148 BINDING 180-180

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
4.6 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINDVISPEFDENGRALRRIRSFVRRQGRLTKGQQLALDSYWPVMGVEYQAAPVDLNTLF
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
GREAPIVLEIGFGMGTSLVTMAANNPQQNFLGIEVHSPGVGACLSSAHDAGLSNLRIMCH
CCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHH
DAVEVLENMIPEASLDMVQLFFPDPWHKARHNKRRIVQTPFVELVKSKLKVGGVFHMATD
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHHHCEEEEECCC
WQPYAEHMLEVMSGVSGYLNLSEQNDYVPRPDSRPLTKFELRGQRLGHGVWDLMFERKE
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHCCCC
>Mature Secondary Structure
MINDVISPEFDENGRALRRIRSFVRRQGRLTKGQQLALDSYWPVMGVEYQAAPVDLNTLF
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCCCEEECCCCCHHHHH
GREAPIVLEIGFGMGTSLVTMAANNPQQNFLGIEVHSPGVGACLSSAHDAGLSNLRIMCH
CCCCCEEEEECCCCCCEEEEEECCCCCCCEEEEEEECCCHHHHHHHHHHCCCCHHHHHHH
DAVEVLENMIPEASLDMVQLFFPDPWHKARHNKRRIVQTPFVELVKSKLKVGGVFHMATD
HHHHHHHHHCCCCCCCEEEEECCCCHHHHHCCCCCEECCHHHHHHHHHHHHCEEEEECCC
WQPYAEHMLEVMSGVSGYLNLSEQNDYVPRPDSRPLTKFELRGQRLGHGVWDLMFERKE
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCHHHHHCCHHHCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA