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The map label for this gene is pckA [H]

Identifier: 153948727

GI number: 153948727

Start: 4462056

End: 4463675

Strand: Direct

Name: pckA [H]

Synonym: YpsIP31758_3978

Alternate gene names: 153948727

Gene position: 4462056-4463675 (Clockwise)

Preceding gene: 153948993

Following gene: 153950179

Centisome position: 94.47

GC content: 49.51

Gene sequence:

>1620_bases
ATGAGTGTTAAAGGAATTACCCCGCAGGAGCTCGCCGCCTATGGCATCCATAACGTCAGCGAGATTGTTTACAACCCAAG
CTATGATTTACTGTTTGAAGAAGAGACCAAACCCACGTTGGAAGGATACGAGCGTGGCACACTGACGACTACCGGCGCAA
TAGCGGTAGATACCGGTATTTTTACCGGGCGTTCACCCAAAGATAAATATATTGTCCGCGATGCTATCACTCAGGATACC
GTGTGGTGGGCCGATCAGGGCAAAGGTAAAAATGATAATAAGCCTCTGAGCCAAGAGATCTGGAGCCATTTGAAAGGTCT
GGTGACGGAACAACTCTCTGGCAAACGCCTCTTTGTTGTCGATACATTCTGCGGTGCTAATGCGGATACCCGCCTGCAAG
TCCGCTTTATCACGGAAGTCGCTTGGCAGGCACACTTCGTCAAAAATATGTTTATCCGTCCATCAGATGAAGAGCTGGCT
CGGTTCGAGCCTGACTTTATCGTGATGAACGGTGCCAAATGCACTAACCCACAATGGAAAGAGCAGGGCCTGAACTCAGA
AAACTTTGTCGCCTTTAATCTGACAGAACGTATGCAGTTGATTGGTGGCACGTGGTATGGCGGCGAAATGAAGAAAGGGA
TGTTCTCAATGATGAACTACCTGCTGCCACTGAAAGGCATTGCTTCAATGCATTGTTCAGCTAACGTCGGCGAAAAAGGC
GATGTTGCCATCTTCTTCGGTCTTTCGGGTACCGGTAAAACCACTTTATCTACCGATCCAAAACGCAAGTTGATCGGTGA
TGATGAACATGGCTGGGATGATGATGGCGTCTTTAACTTCGAGGGGGGGTGCTACGCTAAAACCATCAAGTTATCTGAAG
AAGCAGAGCCAGATATTTACCACGCCATTAAACGCGACGCCTTGCTGGAAAACGTGGTGGTGCTAGCAGACGGCACCGTT
GATTTTAATGACGGTTCTAAAACCGAAAACACCCGCGTCTCTTATCCGATTTACCACATTGATAACATCGTTAAACCGGT
GTCTAAAGCAGGCCATGCGACCAAGGTTATCTTCTTGACTGCCGATGCTTTTGGGGTGCTCCCCCCAGTATCTCGTCTGA
CCGCAAACCAAACTCAATATCACTTCCTATCTGGCTTTACTGCCAAACTGGCAGGGACAGAGCGTGGCGTCACGGAGCCA
ACACCAACTTTCTCTGCTTGCTTTGGTGCGGCCTTCCTATCGCTGCACCCAACGCAGTACGCTGAAGTGCTGGTTAAGCG
TATGCAAGCGGTTGGCGCACAAGCCTATCTGGTCAATACCGGTTGGAATGGGACAGGTAAGCGTATTTCCATCAAGGATA
CCCGCGCCATTATTGACGCAATCCTAAACGGGGAGATTGATAAGGCAGAAACCTTTACACTGCCAATCTTTGATCTGGCA
GTCCCTATGTCGTTACCCGGTGTGAATCCCGATATCCTCGATCCTCGCGACACCTACGCCGATAAAGCACAATGGCAAGA
GAAAGCTGAAGATTTGGCGAAGCGCTTTGCGACTAACTTTGATAAATACACTGATACCCCTGCGGGGGCCGCGTTGGTTA
GCGCGGGGCCAAAGATCTAA

Upstream 100 bases:

>100_bases
AAAATAGGTCATATATTAAAATATTTGATAGCTATCGCTGTTAATCAGAGATGACTACTTACAATCGCTGGTAGAAAATT
GACGATCCAGGAGTTATGAC

Downstream 100 bases:

>100_bases
GCCAGTGTTAGCTGTTTTAGTTTAGCGTTACAGGGGGGCACTTTCAGAATTGTGCCCCTTTTTTCTATTTATTTATAAAA
CCACTGATTTATTTATAAAA

Product: phosphoenolpyruvate carboxykinase

Products: NA

Alternate protein names: PEP carboxykinase; PEPCK; Phosphoenolpyruvate carboxylase [H]

Number of amino acids: Translated: 539; Mature: 538

Protein sequence:

>539_residues
MSVKGITPQELAAYGIHNVSEIVYNPSYDLLFEEETKPTLEGYERGTLTTTGAIAVDTGIFTGRSPKDKYIVRDAITQDT
VWWADQGKGKNDNKPLSQEIWSHLKGLVTEQLSGKRLFVVDTFCGANADTRLQVRFITEVAWQAHFVKNMFIRPSDEELA
RFEPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKG
DVAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTV
DFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLSLHPTQYAEVLVKRMQAVGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGEIDKAETFTLPIFDLA
VPMSLPGVNPDILDPRDTYADKAQWQEKAEDLAKRFATNFDKYTDTPAGAALVSAGPKI

Sequences:

>Translated_539_residues
MSVKGITPQELAAYGIHNVSEIVYNPSYDLLFEEETKPTLEGYERGTLTTTGAIAVDTGIFTGRSPKDKYIVRDAITQDT
VWWADQGKGKNDNKPLSQEIWSHLKGLVTEQLSGKRLFVVDTFCGANADTRLQVRFITEVAWQAHFVKNMFIRPSDEELA
RFEPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKG
DVAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTV
DFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEP
TPTFSACFGAAFLSLHPTQYAEVLVKRMQAVGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGEIDKAETFTLPIFDLA
VPMSLPGVNPDILDPRDTYADKAQWQEKAEDLAKRFATNFDKYTDTPAGAALVSAGPKI
>Mature_538_residues
SVKGITPQELAAYGIHNVSEIVYNPSYDLLFEEETKPTLEGYERGTLTTTGAIAVDTGIFTGRSPKDKYIVRDAITQDTV
WWADQGKGKNDNKPLSQEIWSHLKGLVTEQLSGKRLFVVDTFCGANADTRLQVRFITEVAWQAHFVKNMFIRPSDEELAR
FEPDFIVMNGAKCTNPQWKEQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKGD
VAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIYHAIKRDALLENVVVLADGTVD
FNDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIFLTADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEPT
PTFSACFGAAFLSLHPTQYAEVLVKRMQAVGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGEIDKAETFTLPIFDLAV
PMSLPGVNPDILDPRDTYADKAQWQEKAEDLAKRFATNFDKYTDTPAGAALVSAGPKI

Specific function: Rate-limiting gluconeogenic enzyme. [C]

COG id: COG1866

COG function: function code C; Phosphoenolpyruvate carboxykinase (ATP)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoenolpyruvate carboxykinase [ATP] family [H]

Homologues:

Organism=Escherichia coli, GI1789807, Length=535, Percent_Identity=87.8504672897196, Blast_Score=990, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6322950, Length=541, Percent_Identity=44.362292051756, Blast_Score=439, Evalue=1e-124,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001272
- InterPro:   IPR013035
- InterPro:   IPR008210
- InterPro:   IPR015994 [H]

Pfam domain/function: PF01293 PEPCK_ATP [H]

EC number: =4.1.1.49 [H]

Molecular weight: Translated: 59338; Mature: 59207

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00532 PEPCK_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKGITPQELAAYGIHNVSEIVYNPSYDLLFEEETKPTLEGYERGTLTTTGAIAVDTGI
CCCCCCCHHHHHHHCCCHHHHHEECCCCCEEEECCCCCCHHCCCCCCEEECCEEEEECCE
FTGRSPKDKYIVRDAITQDTVWWADQGKGKNDNKPLSQEIWSHLKGLVTEQLSGKRLFVV
ECCCCCCCCEEEHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
DTFCGANADTRLQVRFITEVAWQAHFVKNMFIRPSDEELARFEPDFIVMNGAKCTNPQWK
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCHH
EQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKG
HCCCCCCCEEEEEHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIY
CEEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHH
HAIKRDALLENVVVLADGTVDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIFLT
HHHHHHHHHCCEEEEECCEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLSLHPTQY
CCCCCCCCCHHHHHCCCCHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
AEVLVKRMQAVGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGEIDKAETFTLPIFDLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEEHHHHC
VPMSLPGVNPDILDPRDTYADKAQWQEKAEDLAKRFATNFDKYTDTPAGAALVSAGPKI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCCCCC
>Mature Secondary Structure 
SVKGITPQELAAYGIHNVSEIVYNPSYDLLFEEETKPTLEGYERGTLTTTGAIAVDTGI
CCCCCCHHHHHHHCCCHHHHHEECCCCCEEEECCCCCCHHCCCCCCEEECCEEEEECCE
FTGRSPKDKYIVRDAITQDTVWWADQGKGKNDNKPLSQEIWSHLKGLVTEQLSGKRLFVV
ECCCCCCCCEEEHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEE
DTFCGANADTRLQVRFITEVAWQAHFVKNMFIRPSDEELARFEPDFIVMNGAKCTNPQWK
EECCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCHHHHHHCCCCEEEECCCCCCCCCHH
EQGLNSENFVAFNLTERMQLIGGTWYGGEMKKGMFSMMNYLLPLKGIASMHCSANVGEKG
HCCCCCCCEEEEEHHHHHHHHCCCEECCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
DVAIFFGLSGTGKTTLSTDPKRKLIGDDEHGWDDDGVFNFEGGCYAKTIKLSEEAEPDIY
CEEEEEEECCCCCCCCCCCCCHHCCCCCCCCCCCCCEEEECCCCEEEEEEECCCCCCHHH
HAIKRDALLENVVVLADGTVDFNDGSKTENTRVSYPIYHIDNIVKPVSKAGHATKVIFLT
HHHHHHHHHCCEEEEECCEEECCCCCCCCCCEEECCHHHHHHHHHHHHHCCCCEEEEEEE
ADAFGVLPPVSRLTANQTQYHFLSGFTAKLAGTERGVTEPTPTFSACFGAAFLSLHPTQY
CCCCCCCCCHHHHHCCCCHHHHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHCCCHHH
AEVLVKRMQAVGAQAYLVNTGWNGTGKRISIKDTRAIIDAILNGEIDKAETFTLPIFDLA
HHHHHHHHHHCCCEEEEEECCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCEEEEEHHHHC
VPMSLPGVNPDILDPRDTYADKAQWQEKAEDLAKRFATNFDKYTDTPAGAALVSAGPKI
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA