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The map label for this gene is purU [H]

Identifier: 153948690

GI number: 153948690

Start: 2274529

End: 2275377

Strand: Direct

Name: purU [H]

Synonym: YpsIP31758_1971

Alternate gene names: 153948690

Gene position: 2274529-2275377 (Clockwise)

Preceding gene: 153949789

Following gene: 153949149

Centisome position: 48.16

GC content: 42.76

Gene sequence:

>849_bases
ATGCCACACCAGAATGTACAAAAGAAAGTATTGCGCACTATTTGCCCTGACGCTAAAGGTTTGATCGCAAAGATCACTAA
TATCTGCTACAAGCACCAATTGAATATCGTACAAAATAATGAATTTGTTGATCACCTGACTGGTCGCTTCTTTATGCGCA
CCGAGCTTGAGGGGATTTTTAACGATACGACATTACTGGCAGATCTTGACGATGCATTACCTGAAGGCACTAATCGCGAG
TTACACGTTGCTGGGCGCCGCCGCATCATCATTATGGTAACGAAAGAAGCACATTGCCTTGGCGACTTATTAATGAAGAG
TGCTTATGGTGGCTTAGATGTAGAAATTGCGGCAGTCATTGGTAACCATGATGCATTGCAAAATTTAGTCGAGCGTTTTG
ATATCCCATTCCATCTGGTCAGCCATGAAGGCCTCACCCGTGAGCAGCATGATCAACAGTTGATTGAGAAAATTGAACAA
TATCAACCAGATTATGTTGTTCTGGCTAAATATATGCGGGTGCTAACACCGGCCTTTGTGCAACGTTTCCCTTATCAAAT
TATTAATATTCATCATTCATTTTTGCCTGCATTTATCGGTGCTCGGCCATACCACCAAGCGTATGAACGCGGAGTGAAGA
TTATTGGCGCTACTGCTCATTACGTAAATGATAGCTTGGATGAAGGCCCAATCATCATGCAGGACGTGATCAATGTGGAT
CATTCTTATACTGCTGAAGATATGATGCGTGCTGGTCGTGATGTAGAAAAGAATGTTCTCAGTAGTGCGCTATACCGTGT
TCTCACTCAACGTGTTTTTGTTTACGGTAACCGCACTGTTATCCTATAA

Upstream 100 bases:

>100_bases
AATGCTGTGGCCGTTAATTAAACTATTTCTTTATTGTTACTCCTAAAATATCAGACACTTACACAACATGAAATAATGTT
AAGACAGGATTTACCACCTT

Downstream 100 bases:

>100_bases
AGTGATGTTTTTTGTCTAGATATCCATCTTCACGGACAAAAAAGCACCGAACGAATTACTTTTTTCATTTCAAGTCTTTA
CAGGGGCGCTTCATTTGATA

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 282; Mature: 281

Protein sequence:

>282_residues
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRE
LHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQ
YQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HSYTAEDMMRAGRDVEKNVLSSALYRVLTQRVFVYGNRTVIL

Sequences:

>Translated_282_residues
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNRE
LHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQ
YQPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HSYTAEDMMRAGRDVEKNVLSSALYRVLTQRVFVYGNRTVIL
>Mature_281_residues
PHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIFNDTTLLADLDDALPEGTNREL
HVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVIGNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQY
QPDYVVLAKYMRVLTPAFVQRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVDH
SYTAEDMMRAGRDVEKNVLSSALYRVLTQRVFVYGNRTVIL

Specific function: Produces formate from formyl-tetrahydrofolate. Provides the major source of formate for the purT-dependent synthesis of 5'-phosphoribosyl-N-formylglycinamide (FGAR) during aerobic growth. Has a role in regulating the one-carbon pool [H]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869995, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Homo sapiens, GI209869993, Length=190, Percent_Identity=29.4736842105263, Blast_Score=94, Evalue=1e-19,
Organism=Escherichia coli, GI1787483, Length=279, Percent_Identity=84.9462365591398, Blast_Score=500, Evalue=1e-143,
Organism=Escherichia coli, GI1788846, Length=103, Percent_Identity=41.747572815534, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI17567511, Length=163, Percent_Identity=33.1288343558282, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI24582400, Length=159, Percent_Identity=29.559748427673, Blast_Score=82, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 32234; Mature: 32102

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIF
CCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
NDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCC
HSYTAEDMMRAGRDVEKNVLSSALYRVLTQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEC
>Mature Secondary Structure 
PHQNVQKKVLRTICPDAKGLIAKITNICYKHQLNIVQNNEFVDHLTGRFFMRTELEGIF
CCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCEEECCHHHHHHHHHHHHHHHHHHHH
NDTTLLADLDDALPEGTNRELHVAGRRRIIIMVTKEAHCLGDLLMKSAYGGLDVEIAAVI
CCCHHEEECHHHCCCCCCCEEEECCCCEEEEEEECCHHHHHHHHHHHHCCCCCEEEEEEC
GNHDALQNLVERFDIPFHLVSHEGLTREQHDQQLIEKIEQYQPDYVVLAKYMRVLTPAFV
CCHHHHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
QRFPYQIINIHHSFLPAFIGARPYHQAYERGVKIIGATAHYVNDSLDEGPIIMQDVINVD
HHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCEEEEHHHHHHCCCCCCCCCHHHHHHCCC
HSYTAEDMMRAGRDVEKNVLSSALYRVLTQRVFVYGNRTVIL
CCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]