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The map label for this gene is bioB [H]

Identifier: 153948619

GI number: 153948619

Start: 3212377

End: 3213414

Strand: Reverse

Name: bioB [H]

Synonym: YpsIP31758_2844

Alternate gene names: 153948619

Gene position: 3213414-3212377 (Counterclockwise)

Preceding gene: 153948838

Following gene: 153947193

Centisome position: 68.03

GC content: 51.16

Gene sequence:

>1038_bases
ATGGCAACTTATCATCACTGGACAGTAGGGCAAGCCCTGGCCCTGTTTGATAAACCGCTGTTGGAATTATTGTTTGAAGC
ACAACAAGTTCACCGCCAGCATTTTGATCCGCGTCAGGTACAAGTCAGTACGTTGTTGTCGATTAAAACCGGTGCCTGTC
CGGAAGATTGTAAATATTGCCCGCAAAGCTCCCGCTATAAAACCGGTCTGGAAAGCGAACGCTTAATGCAGGTTGAGCAG
GTACTGGAATCTGCCAAGAAAGCCAAAGCCGCAGGTTCGACCCGCTTCTGTATGGGCGCGGCGTGGAAAAATCCCCACGA
GAGGGATATGCCTTATTTGGTGAAAATGGTTGAAGGCGTCAAGGCTCTGGGGATGGAAACCTGTATGACATTGGGTTCGC
TGAGTAAACAGCAAGCTCATCGGCTGGCGGATGCCGGGCTGGATTATTACAACCACAATCTGGATACCTCTCCAGAGTTT
TACGGCAGCATTATTACGACCCGCAGCTATCAGGAACGTTTGGATACGTTAAACGAGGTGCGTGACGCTGGGATCAAAGT
GTGTTCTGGTGGCATCGTCGGGCTGGGAGAAACGGTACGCGATCGTGCCGGGCTGTTGGTACAACTGGCGAACTTGCCTA
AGCCACCTGAAAGTGTGCCCATCAATATGTTGGTAAAAGTGAAAGGCACCCCGCTGGAAAATAACGCAGAGGTGGATGCC
TTTGAGTTTATCCGCACCATTGCGGTAGCGCGGATCATGATGCCGTCTTCCTATGTCCGCCTGTCTGCCGGGCGCGAGCA
AATGAACGAACAAACCCAGGCCATGTGCTTTATGGCTGGAGCCAACTCTATTTTCTACGGCTGTAAATTACTGACCACAC
CGAATCCTGATGAAGATAACGATCTGCAATTGTTCCGTAAATTGGGGCTAAACCCGCAGCAGACGGCAACATCACACGGT
GATCGTGAACAACAACAGGCCTTGACCGAGCAGCTACTGCATGGGGATACCGCCCAGTTTTATAATGCGGCGGTCTAA

Upstream 100 bases:

>100_bases
ATTAGGTACCGTTGTAAACCAAAATATCAATAGAATGGTTGACAGTATATCGAGTTTATTTACACTGACGAAACTTTTTC
TCTTCTTTTGAGAGGCCATC

Downstream 100 bases:

>100_bases
TGAGCTGGCAAGATAAAATAGCGCAAGGCTTGCAACGGCGACGTGATGCGGCGGCTTACCGTACTCGCCAGGTCAACGAG
GGGGCCAATGGGCGCTGGTT

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 345; Mature: 344

Protein sequence:

>345_residues
MATYHHWTVGQALALFDKPLLELLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQ
VLESAKKAKAAGSTRFCMGAAWKNPHERDMPYLVKMVEGVKALGMETCMTLGSLSKQQAHRLADAGLDYYNHNLDTSPEF
YGSIITTRSYQERLDTLNEVRDAGIKVCSGGIVGLGETVRDRAGLLVQLANLPKPPESVPINMLVKVKGTPLENNAEVDA
FEFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPDEDNDLQLFRKLGLNPQQTATSHG
DREQQQALTEQLLHGDTAQFYNAAV

Sequences:

>Translated_345_residues
MATYHHWTVGQALALFDKPLLELLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQ
VLESAKKAKAAGSTRFCMGAAWKNPHERDMPYLVKMVEGVKALGMETCMTLGSLSKQQAHRLADAGLDYYNHNLDTSPEF
YGSIITTRSYQERLDTLNEVRDAGIKVCSGGIVGLGETVRDRAGLLVQLANLPKPPESVPINMLVKVKGTPLENNAEVDA
FEFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPDEDNDLQLFRKLGLNPQQTATSHG
DREQQQALTEQLLHGDTAQFYNAAV
>Mature_344_residues
ATYHHWTVGQALALFDKPLLELLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYCPQSSRYKTGLESERLMQVEQV
LESAKKAKAAGSTRFCMGAAWKNPHERDMPYLVKMVEGVKALGMETCMTLGSLSKQQAHRLADAGLDYYNHNLDTSPEFY
GSIITTRSYQERLDTLNEVRDAGIKVCSGGIVGLGETVRDRAGLLVQLANLPKPPESVPINMLVKVKGTPLENNAEVDAF
EFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPDEDNDLQLFRKLGLNPQQTATSHGD
REQQQALTEQLLHGDTAQFYNAAV

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism [H]

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786992, Length=344, Percent_Identity=84.3023255813954, Blast_Score=618, Evalue=1e-178,
Organism=Saccharomyces cerevisiae, GI6321725, Length=318, Percent_Identity=48.4276729559748, Blast_Score=319, Evalue=5e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197 [H]

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM [H]

EC number: =2.8.1.6 [H]

Molecular weight: Translated: 38392; Mature: 38261

Theoretical pI: Translated: 6.60; Mature: 6.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATYHHWTVGQALALFDKPLLELLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCC
PQSSRYKTGLESERLMQVEQVLESAKKAKAAGSTRFCMGAAWKNPHERDMPYLVKMVEGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
KALGMETCMTLGSLSKQQAHRLADAGLDYYNHNLDTSPEFYGSIITTRSYQERLDTLNEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDAGIKVCSGGIVGLGETVRDRAGLLVQLANLPKPPESVPINMLVKVKGTPLENNAEVDA
HHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
FEFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPDEDN
HHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DLQLFRKLGLNPQQTATSHGDREQQQALTEQLLHGDTAQFYNAAV
HHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCC
>Mature Secondary Structure 
ATYHHWTVGQALALFDKPLLELLFEAQQVHRQHFDPRQVQVSTLLSIKTGACPEDCKYC
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHCC
PQSSRYKTGLESERLMQVEQVLESAKKAKAAGSTRFCMGAAWKNPHERDMPYLVKMVEGV
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCHHHHHHHHHHH
KALGMETCMTLGSLSKQQAHRLADAGLDYYNHNLDTSPEFYGSIITTRSYQERLDTLNEV
HHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
RDAGIKVCSGGIVGLGETVRDRAGLLVQLANLPKPPESVPINMLVKVKGTPLENNAEVDA
HHHCCEEECCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEECCCCCCCCCHHH
FEFIRTIAVARIMMPSSYVRLSAGREQMNEQTQAMCFMAGANSIFYGCKLLTTPNPDEDN
HHHHHHHHHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCCEECEEEECCCCCCCCH
DLQLFRKLGLNPQQTATSHGDREQQQALTEQLLHGDTAQFYNAAV
HHHHHHHHCCCHHHHHCCCCCHHHHHHHHHHHHCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA