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The map label for this gene is bepF [H]

Identifier: 153948407

GI number: 153948407

Start: 776623

End: 777813

Strand: Direct

Name: bepF [H]

Synonym: YpsIP31758_0667

Alternate gene names: 153948407

Gene position: 776623-777813 (Clockwise)

Preceding gene: 153949688

Following gene: 153948647

Centisome position: 16.44

GC content: 53.82

Gene sequence:

>1191_bases
ATGGGACCTTTGCGGGCAAAGCAACTCCGCCATTCTTTACTGTCCCATGCGCTTTTTCTGCTCATACTCGGGGCGGGCAC
TGTGAGTGCGGCCCCCGCGCCTTTACCTGCCGTAACCGTTGCGGTAGTCGCGTCAATAACACCAGACAATGCCGTGCAAT
ATCTTGGGCGGATTGAGGCGATTCAAGCGGTTGATGTGACTACCCGCACCGAAGGATTTATCGCCCGCCGTCTGTTTACT
GAAGGGAAAATGGTCAAGCAGGGGGAACTGTTATATGAAATTGATCCCGCTTTGCATCAAGCATCGGTGGCGCAAGCACA
GGCGCAACTCGACAGCGCGACTGCCAGCGCCAATCATGCACAGGTGAACCTCACGCGTTTACAACGCTTGGGTAATAACC
GTTCTGTCAGCCAGGCGGAGGTGGATGAAGCACAGGCACAGCGAGATATCAGCCGGGCAGCGGTGGCGCAGGCGCAGGCT
AACCTACAGATTCAGCAGTTACAGCTCAGCTTCACCCAAATTCATGCGCCAATCAGTGGGCAAATGGGTCACAGCCGTTT
TAATGTGGGCAGCCTGATTAATCCCGCTAGCGGCACCTTGGTCAATATTGTGCAGCTCGATCCGATTCGGGTGGCGATTG
CCGTCAATGAACGGGATTACATTACCGCGACCTATCAACGCGCCGTCGCCCGTGAAAAAAACACTGAAAACCCCTTGCTC
CCTCTGGGGCGTTTTACCCCTCGCATTCAACTGGCAAATGGTAAGCAATATCCAGAGAGTGGTGTTTTTGAATCGGTGGA
TAACCAAATTGATAAGCAGACGGGAACGGTCATTATCCGTACTCGTTTTGCCAACCCTCGCCATTTGTTGCTGCCCGGTG
GCGTAGTCAATGTGTCATTGGCTGCAGACGACGCCAAGCCTGTGACGGTCATCCCGATTGCTGCTTTACAACAGAATAAA
CAGGGCCATTTTGTGCTGGTGGTGACCGATAAAGCGGTGGTCGAAGTGCGCCCAGTGACCCTAGGTGAACAGTTCGAACA
GCAATATGCGGTGACAGATGGGGTGAAGGTGGGTGAAAAAGTCATTGTGGCGGGGCTACAGCATGTCCGACCGGGTATGG
CGGTGAACGCCAGCACGGTTAATGATGGAACTGTAAATGATCGCGTTGCAGCGCCATTGACGGCTAATTAA

Upstream 100 bases:

>100_bases
TACACTGAATTGGGTTCGGTCCACGCCAATTTTTTACGTGCCGCGAACCCTACGCTCTCGGCTAATCTTACGCTCTCCAC
TAATCTTACGCTCTCCGCTA

Downstream 100 bases:

>100_bases
GGGGAAGACCAGATGTTGCATTTCTTTATTCGTCGCCCCAAGTTTGCCATTGTTATCGCATTGGTGATCACCCTGGTTGG
CTGGGTTTCACTGTATGTTA

Product: RND family efflux transporter MFP subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MGPLRAKQLRHSLLSHALFLLILGAGTVSAAPAPLPAVTVAVVASITPDNAVQYLGRIEAIQAVDVTTRTEGFIARRLFT
EGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLGNNRSVSQAEVDEAQAQRDISRAAVAQAQA
NLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRVAIAVNERDYITATYQRAVAREKNTENPLL
PLGRFTPRIQLANGKQYPESGVFESVDNQIDKQTGTVIIRTRFANPRHLLLPGGVVNVSLAADDAKPVTVIPIAALQQNK
QGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRPGMAVNASTVNDGTVNDRVAAPLTAN

Sequences:

>Translated_396_residues
MGPLRAKQLRHSLLSHALFLLILGAGTVSAAPAPLPAVTVAVVASITPDNAVQYLGRIEAIQAVDVTTRTEGFIARRLFT
EGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLGNNRSVSQAEVDEAQAQRDISRAAVAQAQA
NLQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRVAIAVNERDYITATYQRAVAREKNTENPLL
PLGRFTPRIQLANGKQYPESGVFESVDNQIDKQTGTVIIRTRFANPRHLLLPGGVVNVSLAADDAKPVTVIPIAALQQNK
QGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRPGMAVNASTVNDGTVNDRVAAPLTAN
>Mature_395_residues
GPLRAKQLRHSLLSHALFLLILGAGTVSAAPAPLPAVTVAVVASITPDNAVQYLGRIEAIQAVDVTTRTEGFIARRLFTE
GKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHAQVNLTRLQRLGNNRSVSQAEVDEAQAQRDISRAAVAQAQAN
LQIQQLQLSFTQIHAPISGQMGHSRFNVGSLINPASGTLVNIVQLDPIRVAIAVNERDYITATYQRAVAREKNTENPLLP
LGRFTPRIQLANGKQYPESGVFESVDNQIDKQTGTVIIRTRFANPRHLLLPGGVVNVSLAADDAKPVTVIPIAALQQNKQ
GHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEKVIVAGLQHVRPGMAVNASTVNDGTVNDRVAAPLTAN

Specific function: May contribute to resistance to some drugs, such as deoxycholate, sodium dodecyl sulfate and nalidixic acid, in the absence of BepD and BepE [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=349, Percent_Identity=32.9512893982808, Blast_Score=165, Evalue=5e-42,
Organism=Escherichia coli, GI1789929, Length=375, Percent_Identity=29.3333333333333, Blast_Score=161, Evalue=8e-41,
Organism=Escherichia coli, GI1786668, Length=348, Percent_Identity=32.7586206896552, Blast_Score=160, Evalue=1e-40,
Organism=Escherichia coli, GI87082036, Length=322, Percent_Identity=33.5403726708075, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87081796, Length=382, Percent_Identity=23.8219895287958, Blast_Score=65, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42431; Mature: 42300

Theoretical pI: Translated: 9.59; Mature: 9.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.8 %Met     (Mature Protein)
0.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGPLRAKQLRHSLLSHALFLLILGAGTVSAAPAPLPAVTVAVVASITPDNAVQYLGRIEA
CCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHEECCCHHHHHHHHHHHH
IQAVDVTTRTEGFIARRLFTEGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHA
EEEEEEEECCHHHHHHHHHHCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
QVNLTRLQRLGNNRSVSQAEVDEAQAQRDISRAAVAQAQANLQIQQLQLSFTQIHAPISG
EEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHEEEEHHHEECCCCC
QMGHSRFNVGSLINPASGTLVNIVQLDPIRVAIAVNERDYITATYQRAVAREKNTENPLL
CCCCCEECCCCCCCCCCCCEEEEEEECCEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCC
PLGRFTPRIQLANGKQYPESGVFESVDNQIDKQTGTVIIRTRFANPRHLLLPGGVVNVSL
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCEEEEEE
AADDAKPVTVIPIAALQQNKQGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEK
ECCCCCCEEEEEEHHHCCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCHHCHH
VIVAGLQHVRPGMAVNASTVNDGTVNDRVAAPLTAN
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GPLRAKQLRHSLLSHALFLLILGAGTVSAAPAPLPAVTVAVVASITPDNAVQYLGRIEA
CCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEHHEECCCHHHHHHHHHHHH
IQAVDVTTRTEGFIARRLFTEGKMVKQGELLYEIDPALHQASVAQAQAQLDSATASANHA
EEEEEEEECCHHHHHHHHHHCCCEEECCCEEEEECHHHHHHHHHHHHHHHHHHHCCCCCE
QVNLTRLQRLGNNRSVSQAEVDEAQAQRDISRAAVAQAQANLQIQQLQLSFTQIHAPISG
EEEHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEHEEEEHHHEECCCCC
QMGHSRFNVGSLINPASGTLVNIVQLDPIRVAIAVNERDYITATYQRAVAREKNTENPLL
CCCCCEECCCCCCCCCCCCEEEEEEECCEEEEEEECCCCEEHHHHHHHHHHCCCCCCCCC
PLGRFTPRIQLANGKQYPESGVFESVDNQIDKQTGTVIIRTRFANPRHLLLPGGVVNVSL
CCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHCCCEEEEEEECCCCCEEEECCCEEEEEE
AADDAKPVTVIPIAALQQNKQGHFVLVVTDKAVVEVRPVTLGEQFEQQYAVTDGVKVGEK
ECCCCCCEEEEEEHHHCCCCCCCEEEEEECCEEEEEECCCCCHHHHHHHHHHCCCHHCHH
VIVAGLQHVRPGMAVNASTVNDGTVNDRVAAPLTAN
HHHHHHHHCCCCCEEECCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12271122 [H]