Click here to switch to the map view.

The map label for this gene is tkrA [H]

Identifier: 153948249

GI number: 153948249

Start: 4669641

End: 4670621

Strand: Direct

Name: tkrA [H]

Synonym: YpsIP31758_4137

Alternate gene names: 153948249

Gene position: 4669641-4670621 (Clockwise)

Preceding gene: 153949077

Following gene: 153950894

Centisome position: 98.86

GC content: 51.17

Gene sequence:

>981_bases
ATGAAACCTTCTATTGTGTTGTACAAAAGTATTCCCACCGATCTGCATCAGCGTTTAGCACAACATTTTACCGTAAACAG
TTTTGATGGCTTAACACCGGATAATCAGCCGGAGCTATTAGCTGCCCTACAACAAGCGGAAGGATTAATTGGTTCTGGCG
GTAAAATCGATCAAGACTTTTTACAATTAGCGCCAAATTTACGGGCTGCCTCCACCATTTCTGTCGGTTATGACAATTTC
GATGTTGAAGCCCTGAGTCAACGGGGCATTGCACTGATGCACACGCCCACCGTGCTAACAGAGACCGTGGCCGATACCAT
GATGGCGCTCATGTTGTCTACGGCGCGTCGGGTGGTTGAACTGGCGGAGCGGGTGAAAGCCGGTGAATGGCAAGAGAGCA
TTGGTGATGACTGGTTCGGTGTTGACGTTCACCATAAAACCATTGGTATTCTGGGCATGGGCCGTATTGGCATGGCGTTA
GCCCAACGGGCACATTTTGGTTTCAGTATGCCCGTGCTGTACACCAGCCGCCGCCCCCACGAAGCAGCAGAACAGCGTTT
TGGTGCCCGCCACTGCTCACTGGATACTCTGCTAGCTGAGGCCGATTTCCTGTGTATTACCTTGCCGATGACAGAACAGA
CTTATCATATGATTGGCCGTGAACAACTGGCAAAAATGAAATCCAGTGCCATTTTGATTAATGCGGGCCGTGGACCCGTG
GTGGATGAACAGGCGCTGATTGCAGCCTTACAAGATGGCACGATCCACGCCGCGGGGCTGGATGTCTTTGAGCAAGAGCC
GCTGCCTGTGGATTCGCCACTCCTGACGCTACGTAATGTGGTTGCTGTACCACACATTGGTTCTGCCACCCATGAAACCC
GCTACAACATGGCGGCTTGTGCAGTTGATAACCTCATCAATGCCCTCACCGGCACGGTAAAAGAGAACTGTGTAAACCCA
CAGGTGCTAATAACTCACTAA

Upstream 100 bases:

>100_bases
GCAACTAAGCGCCACCCAAGAACTGACATTAAGTCACTGACTGGGATGACAACGCACAGGTAATTTGAAATATGACGGGT
ATAACTGGCTGGAGTGTGTG

Downstream 100 bases:

>100_bases
ATTAACATCCGCCCGTCAATTCTATTGGCCTGCCCTGTTTAGCGGGTCAATAGATGTTTGGCAGGCCAGTAGATGTTTAG
TAGGCCAATAACGTTTTGCC

Product: 2-ketogluconate reductase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MKPSIVLYKSIPTDLHQRLAQHFTVNSFDGLTPDNQPELLAALQQAEGLIGSGGKIDQDFLQLAPNLRAASTISVGYDNF
DVEALSQRGIALMHTPTVLTETVADTMMALMLSTARRVVELAERVKAGEWQESIGDDWFGVDVHHKTIGILGMGRIGMAL
AQRAHFGFSMPVLYTSRRPHEAAEQRFGARHCSLDTLLAEADFLCITLPMTEQTYHMIGREQLAKMKSSAILINAGRGPV
VDEQALIAALQDGTIHAAGLDVFEQEPLPVDSPLLTLRNVVAVPHIGSATHETRYNMAACAVDNLINALTGTVKENCVNP
QVLITH

Sequences:

>Translated_326_residues
MKPSIVLYKSIPTDLHQRLAQHFTVNSFDGLTPDNQPELLAALQQAEGLIGSGGKIDQDFLQLAPNLRAASTISVGYDNF
DVEALSQRGIALMHTPTVLTETVADTMMALMLSTARRVVELAERVKAGEWQESIGDDWFGVDVHHKTIGILGMGRIGMAL
AQRAHFGFSMPVLYTSRRPHEAAEQRFGARHCSLDTLLAEADFLCITLPMTEQTYHMIGREQLAKMKSSAILINAGRGPV
VDEQALIAALQDGTIHAAGLDVFEQEPLPVDSPLLTLRNVVAVPHIGSATHETRYNMAACAVDNLINALTGTVKENCVNP
QVLITH
>Mature_326_residues
MKPSIVLYKSIPTDLHQRLAQHFTVNSFDGLTPDNQPELLAALQQAEGLIGSGGKIDQDFLQLAPNLRAASTISVGYDNF
DVEALSQRGIALMHTPTVLTETVADTMMALMLSTARRVVELAERVKAGEWQESIGDDWFGVDVHHKTIGILGMGRIGMAL
AQRAHFGFSMPVLYTSRRPHEAAEQRFGARHCSLDTLLAEADFLCITLPMTEQTYHMIGREQLAKMKSSAILINAGRGPV
VDEQALIAALQDGTIHAAGLDVFEQEPLPVDSPLLTLRNVVAVPHIGSATHETRYNMAACAVDNLINALTGTVKENCVNP
QVLITH

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG1052

COG function: function code CHR; Lactate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrB subfamily [H]

Homologues:

Organism=Homo sapiens, GI6912396, Length=323, Percent_Identity=39.938080495356, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI23308577, Length=292, Percent_Identity=30.4794520547945, Blast_Score=148, Evalue=5e-36,
Organism=Homo sapiens, GI145580578, Length=269, Percent_Identity=31.9702602230483, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI4557499, Length=269, Percent_Identity=31.9702602230483, Blast_Score=134, Evalue=1e-31,
Organism=Homo sapiens, GI145580575, Length=269, Percent_Identity=31.9702602230483, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI4557497, Length=263, Percent_Identity=31.1787072243346, Blast_Score=124, Evalue=2e-28,
Organism=Homo sapiens, GI61743967, Length=263, Percent_Identity=31.1787072243346, Blast_Score=123, Evalue=2e-28,
Organism=Escherichia coli, GI87082289, Length=322, Percent_Identity=71.1180124223602, Blast_Score=488, Evalue=1e-139,
Organism=Escherichia coli, GI1787645, Length=191, Percent_Identity=29.8429319371728, Blast_Score=106, Evalue=2e-24,
Organism=Escherichia coli, GI1789279, Length=258, Percent_Identity=30.6201550387597, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI87081824, Length=157, Percent_Identity=36.3057324840764, Blast_Score=83, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17532191, Length=285, Percent_Identity=33.6842105263158, Blast_Score=143, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI25147481, Length=282, Percent_Identity=25.531914893617, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324055, Length=286, Percent_Identity=32.8671328671329, Blast_Score=162, Evalue=6e-41,
Organism=Saccharomyces cerevisiae, GI6320925, Length=304, Percent_Identity=30.5921052631579, Blast_Score=122, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6322116, Length=289, Percent_Identity=30.4498269896194, Blast_Score=118, Evalue=1e-27,
Organism=Saccharomyces cerevisiae, GI6325144, Length=178, Percent_Identity=35.3932584269663, Blast_Score=97, Evalue=4e-21,
Organism=Saccharomyces cerevisiae, GI6324964, Length=253, Percent_Identity=27.2727272727273, Blast_Score=90, Evalue=5e-19,
Organism=Saccharomyces cerevisiae, GI6321253, Length=280, Percent_Identity=27.5, Blast_Score=89, Evalue=8e-19,
Organism=Drosophila melanogaster, GI28574286, Length=283, Percent_Identity=40.9893992932862, Blast_Score=206, Evalue=1e-53,
Organism=Drosophila melanogaster, GI45551003, Length=283, Percent_Identity=39.5759717314488, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI24585514, Length=283, Percent_Identity=39.5759717314488, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28574282, Length=283, Percent_Identity=39.5759717314488, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI45552429, Length=284, Percent_Identity=40.1408450704225, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28574284, Length=283, Percent_Identity=39.5759717314488, Blast_Score=194, Evalue=9e-50,
Organism=Drosophila melanogaster, GI28571528, Length=298, Percent_Identity=39.9328859060403, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24585516, Length=247, Percent_Identity=36.8421052631579, Blast_Score=160, Evalue=9e-40,
Organism=Drosophila melanogaster, GI24646446, Length=268, Percent_Identity=32.4626865671642, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646448, Length=268, Percent_Identity=32.4626865671642, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646452, Length=268, Percent_Identity=32.4626865671642, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24646450, Length=268, Percent_Identity=32.4626865671642, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI62472511, Length=268, Percent_Identity=32.4626865671642, Blast_Score=125, Evalue=6e-29,
Organism=Drosophila melanogaster, GI19921140, Length=249, Percent_Identity=29.718875502008, Blast_Score=120, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006139
- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00389 2-Hacid_dh; PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 35465; Mature: 35465

Theoretical pI: Translated: 5.69; Mature: 5.69

Prosite motif: PS00065 D_2_HYDROXYACID_DH_1 ; PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSIVLYKSIPTDLHQRLAQHFTVNSFDGLTPDNQPELLAALQQAEGLIGSGGKIDQDF
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
LQLAPNLRAASTISVGYDNFDVEALSQRGIALMHTPTVLTETVADTMMALMLSTARRVVE
HHHCCCCCCCEEEEECCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LAERVKAGEWQESIGDDWFGVDVHHKTIGILGMGRIGMALAQRAHFGFSMPVLYTSRRPH
HHHHHCCCCHHHHHCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCEEEECCCCH
EAAEQRFGARHCSLDTLLAEADFLCITLPMTEQTYHMIGREQLAKMKSSAILINAGRGPV
HHHHHHHCCHHCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
VDEQALIAALQDGTIHAAGLDVFEQEPLPVDSPLLTLRNVVAVPHIGSATHETRYNMAAC
CCHHHHHHHHCCCEEEECCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AVDNLINALTGTVKENCVNPQVLITH
HHHHHHHHHHHHHHHHCCCCEEEEEC
>Mature Secondary Structure
MKPSIVLYKSIPTDLHQRLAQHFTVNSFDGLTPDNQPELLAALQQAEGLIGSGGKIDQDF
CCCCEEEEECCCHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCHHH
LQLAPNLRAASTISVGYDNFDVEALSQRGIALMHTPTVLTETVADTMMALMLSTARRVVE
HHHCCCCCCCEEEEECCCCCCHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHH
LAERVKAGEWQESIGDDWFGVDVHHKTIGILGMGRIGMALAQRAHFGFSMPVLYTSRRPH
HHHHHCCCCHHHHHCCCEEEEEEECCEEEEEECCHHHHHHHHHHHCCCCCCEEEECCCCH
EAAEQRFGARHCSLDTLLAEADFLCITLPMTEQTYHMIGREQLAKMKSSAILINAGRGPV
HHHHHHHCCHHCCHHHHHHCCCEEEEEECCCHHHHHHHHHHHHHHHCCCEEEEECCCCCC
VDEQALIAALQDGTIHAAGLDVFEQEPLPVDSPLLTLRNVVAVPHIGSATHETRYNMAAC
CCHHHHHHHHCCCEEEECCCCHHCCCCCCCCCHHHHHHHHHHCCCCCCCCHHHHHHHHHH
AVDNLINALTGTVKENCVNPQVLITH
HHHHHHHHHHHHHHHHCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA