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The map label for this gene is ompF [H]

Identifier: 153948152

GI number: 153948152

Start: 3139451

End: 3140566

Strand: Direct

Name: ompF [H]

Synonym: YpsIP31758_2778

Alternate gene names: 153948152

Gene position: 3139451-3140566 (Clockwise)

Preceding gene: 153947252

Following gene: 153950004

Centisome position: 66.47

GC content: 41.58

Gene sequence:

>1116_bases
ATGAAACGTAATATTCTGGCAATCCTTATACCGACATTATTAGTTGCAACCACTTCACATGCGGCAGAAATTTACAACAA
AGACGGTAATAAGCTGGATTTATATGGAAAAGTAAAAGCGTTACATTACTTTTCTGATAATACAAAAAGTGATGGTGATA
AATCGTATGTTCGCCTTGGTTTCAAAGGTGTCACTCAAATAACGGATGAACTGAGCGGTTACGGCCAGTGGGAATATAAC
TTCGCGGCTAACTATGCCGAGAGCCAAGAAGCAAAAGACAATAAAACACGCTTAGCCTTTGCCGGTTTACGTTACGGTAA
TTTAGGTTCAATCGACTATGGCCGTAACTACGGCGTGTTATATGATATTGCAGCCTGGACCGATATGCTGCCTGAGTTCG
GGAATGACAGCTACACCAGAACCGATAATTTTATGACTGGCCGTACCACGGGCGTTGCTACCTACCGCAACACAGATTTC
TTTGGTTTGGTTGATGGTCTGAAATTCTCTCTGCAATATCAAGGCAAGAACGGTGCCGAAGGCGAGACGAATAACGGGCG
TGCTGATACATCCAAGCAGAATGGCGACGGTTTCGGTTTATCTTCCAGCTATGAGATTGGCGCAGGTGTCAGCGTAGGTG
CAGCCTATGCTTCATCTAACCGCACCCTAGCCCAGAAGAACAGTACCTTTGGTAAAGGCGATAAAGCCGATGCCTGGACT
ACCGGCCTGAAATATAGCGATAACGGTGTTTATTTAGCGGCCAATTATGCAGAAACGCGCAATATGACACCAATCTCTGG
TACTGCAGTAATTAATAACGTGTCAACCAGCGTAAGTGGTTTTTCTAATAAAACTCAAAACATTGAGTTAGTGGCTCAAT
ATTTATTTGACTTTGGTTTAAAACCTTCCATTGCCTATATTCAATCTAAAGGAAAAGATATCGAAGGCATTGGTGACACC
GACCTGGTTAAATATGTCGATATTGGTGCAACATATTACTTCAACAAAAACATGTCCACGTATGTTGATTATAAAATAAA
CCAACTCAATGATGATAATAAACTAAAATTGAATACTGATAACGTTGTAGCACTTGGTCTGGTGTATCAGTTCTAA

Upstream 100 bases:

>100_bases
GATGCGGTAAAAATTCTGCGCTGGGAAAATTCAGACAGATATTGAATACAGCAAAAATTTTATTATTCGCGTTTTTTCAA
TAAGAGAAACAGGGCAAAAC

Downstream 100 bases:

>100_bases
TTTGTATGTAAGGTGTTACCCTCATTTTATTGTGAATTATGGTTCTTTGAATGTATTTATTATGCCGGGATTATTCCCGG
CTTTTTTACGCCACTCGCCT

Product: outer membrane protein N

Products: NA

Alternate protein names: Porin ompF [H]

Number of amino acids: Translated: 371; Mature: 371

Protein sequence:

>371_residues
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLGFKGVTQITDELSGYGQWEYN
FAANYAESQEAKDNKTRLAFAGLRYGNLGSIDYGRNYGVLYDIAAWTDMLPEFGNDSYTRTDNFMTGRTTGVATYRNTDF
FGLVDGLKFSLQYQGKNGAEGETNNGRADTSKQNGDGFGLSSSYEIGAGVSVGAAYASSNRTLAQKNSTFGKGDKADAWT
TGLKYSDNGVYLAANYAETRNMTPISGTAVINNVSTSVSGFSNKTQNIELVAQYLFDFGLKPSIAYIQSKGKDIEGIGDT
DLVKYVDIGATYYFNKNMSTYVDYKINQLNDDNKLKLNTDNVVALGLVYQF

Sequences:

>Translated_371_residues
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLGFKGVTQITDELSGYGQWEYN
FAANYAESQEAKDNKTRLAFAGLRYGNLGSIDYGRNYGVLYDIAAWTDMLPEFGNDSYTRTDNFMTGRTTGVATYRNTDF
FGLVDGLKFSLQYQGKNGAEGETNNGRADTSKQNGDGFGLSSSYEIGAGVSVGAAYASSNRTLAQKNSTFGKGDKADAWT
TGLKYSDNGVYLAANYAETRNMTPISGTAVINNVSTSVSGFSNKTQNIELVAQYLFDFGLKPSIAYIQSKGKDIEGIGDT
DLVKYVDIGATYYFNKNMSTYVDYKINQLNDDNKLKLNTDNVVALGLVYQF
>Mature_371_residues
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLGFKGVTQITDELSGYGQWEYN
FAANYAESQEAKDNKTRLAFAGLRYGNLGSIDYGRNYGVLYDIAAWTDMLPEFGNDSYTRTDNFMTGRTTGVATYRNTDF
FGLVDGLKFSLQYQGKNGAEGETNNGRADTSKQNGDGFGLSSSYEIGAGVSVGAAYASSNRTLAQKNSTFGKGDKADAWT
TGLKYSDNGVYLAANYAETRNMTPISGTAVINNVSTSVSGFSNKTQNIELVAQYLFDFGLKPSIAYIQSKGKDIEGIGDT
DLVKYVDIGATYYFNKNMSTYVDYKINQLNDDNKLKLNTDNVVALGLVYQF

Specific function: OmpF is a porin that forms passive diffusion pores which allow small molecular weight hydrophilic materials across the outer membrane. It is also a receptor for the bacteriophage T2 [H]

COG id: COG3203

COG function: function code M; Outer membrane protein (porin)

Gene ontology:

Cell location: Cell outer membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Gram-negative porin family [H]

Homologues:

Organism=Escherichia coli, GI1786436, Length=372, Percent_Identity=60.2150537634409, Blast_Score=454, Evalue=1e-129,
Organism=Escherichia coli, GI1787641, Length=367, Percent_Identity=62.3978201634877, Blast_Score=440, Evalue=1e-125,
Organism=Escherichia coli, GI1787160, Length=377, Percent_Identity=59.6816976127321, Blast_Score=431, Evalue=1e-122,
Organism=Escherichia coli, GI1788544, Length=384, Percent_Identity=58.59375, Blast_Score=420, Evalue=1e-119,

Paralogues:

None

Copy number: 794 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001897
- InterPro:   IPR013793
- InterPro:   IPR001702 [H]

Pfam domain/function: PF00267 Porin_1 [H]

EC number: NA

Molecular weight: Translated: 40694; Mature: 40694

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00576 GRAM_NEG_PORIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLG
CCCCEEHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEC
FKGVTQITDELSGYGQWEYNFAANYAESQEAKDNKTRLAFAGLRYGNLGSIDYGRNYGVL
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEE
YDIAAWTDMLPEFGNDSYTRTDNFMTGRTTGVATYRNTDFFGLVDGLKFSLQYQGKNGAE
EEHHHHHHHHHHHCCCCCEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCC
GETNNGRADTSKQNGDGFGLSSSYEIGAGVSVGAAYASSNRTLAQKNSTFGKGDKADAWT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECEEEECCCCEEEECCCCCCCCCCCCHHC
TGLKYSDNGVYLAANYAETRNMTPISGTAVINNVSTSVSGFSNKTQNIELVAQYLFDFGL
CCCEECCCCEEEEEECHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
KPSIAYIQSKGKDIEGIGDTDLVKYVDIGATYYFNKNMSTYVDYKINQLNDDNKLKLNTD
CCCCCEECCCCCCCCCCCCCCCEEEEECCEEEEECCCCCEEEEEEEEECCCCCEEEECCC
NVVALGLVYQF
CEEEEEEEECC
>Mature Secondary Structure
MKRNILAILIPTLLVATTSHAAEIYNKDGNKLDLYGKVKALHYFSDNTKSDGDKSYVRLG
CCCCEEHHHHHHHHHHCCHHHHHHHCCCCCEEEEEEEEEEEEEECCCCCCCCCCEEEEEC
FKGVTQITDELSGYGQWEYNFAANYAESQEAKDNKTRLAFAGLRYGNLGSIDYGRNYGVL
CCHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCEE
YDIAAWTDMLPEFGNDSYTRTDNFMTGRTTGVATYRNTDFFGLVDGLKFSLQYQGKNGAE
EEHHHHHHHHHHHCCCCCEECCCEECCCCCEEEEEECCCEEEEECCEEEEEEECCCCCCC
GETNNGRADTSKQNGDGFGLSSSYEIGAGVSVGAAYASSNRTLAQKNSTFGKGDKADAWT
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECEEEECCCCEEEECCCCCCCCCCCCHHC
TGLKYSDNGVYLAANYAETRNMTPISGTAVINNVSTSVSGFSNKTQNIELVAQYLFDFGL
CCCEECCCCEEEEEECHHHCCCCCCCCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHCCC
KPSIAYIQSKGKDIEGIGDTDLVKYVDIGATYYFNKNMSTYVDYKINQLNDDNKLKLNTD
CCCCCEECCCCCCCCCCCCCCCEEEEECCEEEEECCCCCEEEEEEEEECCCCCEEEECCC
NVVALGLVYQF
CEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9274033 [H]