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The map label for this gene is sseA [H]

Identifier: 153948060

GI number: 153948060

Start: 3151344

End: 3152198

Strand: Direct

Name: sseA [H]

Synonym: YpsIP31758_2787

Alternate gene names: 153948060

Gene position: 3151344-3152198 (Clockwise)

Preceding gene: 153950571

Following gene: 153947856

Centisome position: 66.72

GC content: 51.11

Gene sequence:

>855_bases
ATGAACTCAGATTTTCTGGTTACACCGCAATGGCTTACAGCACATGCTAATGATGCCAATATAGTTATTTTGGATGCCAG
AATGTCGCCTCCCGGAGTGGTCCCCAAACGTAATATTCAGGCTGAGTTTGAACAGGGCCATATTCCCGGCGCGGTCTATT
TTGATATTGATGCAATAGCAGATCACAGTACCGGCTTACCCCATATGCTGCCCTCTCCTCAGCTATTCAGTGAAATGGCA
GGGCAGTTAGGCATCACTGAACAACACACGGTGGTGATTTATGATGAGGGTAATCTGTTTTCTGCACCACGCGTGTGGTG
GACGTTCCGCACATTTGGCGCTAAAAATGTGCGTATTCTGGCAAGTGGTCTGAGCGGGTGGCAACAAGCAGGCTATAAGC
TGGAAAGTGGCCCTGCACACCCTACACCACAAACCTTCAATGTCACTTTCAATGCCGCCGCCGTTTCTAGTGTCGATGAG
GTACTGGCGGTTCTGGGTAATAATGAGGTGCAAATATTGGATGCTCGGCCATCAGCAAGATATAGAGCACAAGAGCCGGA
ACCACGCCCTGGTTTGCGTCTTGGGCGCATCCCTGGCAGCATCAATATACCGTGGGGGACCATGGTGGAGAATGGTCATC
TGAAGTCACCACAGGCATTGGCAGAAATATTTGCGGCTCAAGGGGTGGATTTAACTAAGCCCATTATTACCAGTTGTGGT
TCAGGGGTCACCGCAGCGGTAGTGGTACTGGGTCTAGCGGCGGTGAATGCACGGTCAGTGTCACTCTATGATGGCTCTTG
GGCTGAATGGGGGGCGTCAAACTCTCTACCAATAGACGCAACGCCTCTGGCGTGA

Upstream 100 bases:

>100_bases
ATAAGTCGGGGTTTAATTACAATTATTTCGTTATATAGTGAGTCACCCGATGACGTTATCCTCGGAATTACCCCCTTATA
ACTTTGATAGGTAATATGTT

Downstream 100 bases:

>100_bases
TACCAAAGGCTGGCTCACCACCAGCCACTGACATGACTCATTGCTCATTGCTCATTGCTCATTGCTCATTGTTCATTGTT
CATTGTTCATTGTTCATTGC

Product: 3-mercaptopyruvate sulfurtransferase

Products: NA

Alternate protein names: MST; Rhodanese-like protein [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MNSDFLVTPQWLTAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA

Sequences:

>Translated_284_residues
MNSDFLVTPQWLTAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
>Mature_284_residues
MNSDFLVTPQWLTAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIADHSTGLPHMLPSPQLFSEMA
GQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRILASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDE
VLAVLGNNEVQILDARPSARYRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA

Specific function: Transfers a sulfur ion to cyanide or to other thiol compounds. Also has weak rhodanese activity. Its participation in detoxification of cyanide may be small. May be involved in the enhancement of serine sensitivity [H]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains [H]

Homologues:

Organism=Homo sapiens, GI194473668, Length=272, Percent_Identity=40.4411764705882, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI61835204, Length=272, Percent_Identity=40.4411764705882, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI194473681, Length=272, Percent_Identity=40.4411764705882, Blast_Score=213, Evalue=1e-55,
Organism=Homo sapiens, GI17402865, Length=273, Percent_Identity=36.6300366300366, Blast_Score=191, Evalue=7e-49,
Organism=Escherichia coli, GI87082121, Length=279, Percent_Identity=50.179211469534, Blast_Score=295, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI71997283, Length=306, Percent_Identity=25.1633986928105, Blast_Score=101, Evalue=3e-22,
Organism=Caenorhabditis elegans, GI17561888, Length=287, Percent_Identity=25.0871080139373, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI17543836, Length=296, Percent_Identity=25.3378378378378, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17543838, Length=296, Percent_Identity=25.3378378378378, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17559150, Length=253, Percent_Identity=27.2727272727273, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI115534702, Length=207, Percent_Identity=26.5700483091787, Blast_Score=79, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6324825, Length=243, Percent_Identity=30.4526748971193, Blast_Score=135, Evalue=9e-33,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001763
- InterPro:   IPR001307 [H]

Pfam domain/function: PF00581 Rhodanese [H]

EC number: =2.8.1.2 [H]

Molecular weight: Translated: 30354; Mature: 30354

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS00380 RHODANESE_1 ; PS00683 RHODANESE_2 ; PS50206 RHODANESE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNSDFLVTPQWLTAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIA
CCCCEEECCHHEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHC
DHSTGLPHMLPSPQLFSEMAGQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCEEEEEEEECCCCCEEEE
ASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDEVLAVLGNNEVQILDARPSAR
EECCCHHHHCCEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
CCCCCCCCCCCCEEECCCCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
CCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNSDFLVTPQWLTAHANDANIVILDARMSPPGVVPKRNIQAEFEQGHIPGAVYFDIDAIA
CCCCEEECCHHEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHCCCCCEEEEEEEHHHC
DHSTGLPHMLPSPQLFSEMAGQLGITEQHTVVIYDEGNLFSAPRVWWTFRTFGAKNVRIL
CCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEECCCEEEEEEEECCCCCEEEE
ASGLSGWQQAGYKLESGPAHPTPQTFNVTFNAAAVSSVDEVLAVLGNNEVQILDARPSAR
EECCCHHHHCCEEECCCCCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCEEEEECCCCCC
YRAQEPEPRPGLRLGRIPGSINIPWGTMVENGHLKSPQALAEIFAAQGVDLTKPIITSCG
CCCCCCCCCCCCEEECCCCEEECCCCCEEECCCCCCHHHHHHHHHHCCCCCCHHHHHHCC
SGVTAAVVVLGLAAVNARSVSLYDGSWAEWGASNSLPIDATPLA
CCHHHHHHHHHHHHCCCEEEEEECCCCHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]