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The map label for this gene is fliF [H]

Identifier: 153947902

GI number: 153947902

Start: 741329

End: 742969

Strand: Direct

Name: fliF [H]

Synonym: YpsIP31758_0631

Alternate gene names: 153947902

Gene position: 741329-742969 (Clockwise)

Preceding gene: 153950291

Following gene: 153947831

Centisome position: 15.7

GC content: 50.82

Gene sequence:

>1641_bases
GTGTTAGATAAAATTAAGCAGAAATTGTCACCTGGTCAATTAGGTGAAAATACAAAAACCATTCTTATGAGCGCGGCAGC
CCTTTTGGTTACCGCCGCGATTATTTTCAGCCTCTGGCGTAGCTCACAGGGCTACACGGCGTTGTTTGGCTCGCAGGAGA
ATATCCCGGTCACCCAAGTGGTGGAAGTGCTGGAAGGAGAGGCCATCGCCTACCGTATTAACCCGGATAACGGTCAGGTA
TTGGTGGCTGAAAATCAGTTGGGTAAAGCGCGTATCTTGCTGGCAGCAAAAGGAATTACTGCCACACTCCCCATTGGCTA
TGAGTTGATGGATAAAGAAAGCATGCTTGGCAGCAGCCAGTTTATCCAAAATGTTCGTTATAAACGCAGCCTGGAAGGGG
AACTGGCACAGAGCATGATGGCATTGAGCGCGGTGGAATATGCCCGAGTCCATCTGGGCATGAGTGAAGCCAGTTCATTC
GCCATCAGTAATCGTTCAGATAACAGTGCCTCGGTGGTGTTACGGCTAAGATACGGCCAAACATTGAGCACTGAACAGGT
GGGGGCCATTGTTCAATTGGTGGCGGGCAGTATTCCCGGAATGAAACCTGCCAATGTGCGGGTGGTCGATCAACACGGTG
AATTACTGTCACAAGCTTATCAAGCGAACAGTGAGGGGGTGCCGAGTGTGAAAAGTGGGACCGAATTAGCTCACTATTTG
CAAAGCACCACCGAGAAGAATATCGCAAACCTGTTGAATTCGGTGATTGGCGCGAATAACTACCGCATCAGTGTCTCCAC
TCAATTAGATATGAGTCGTATTGAAGAGACGGCGGAGCGCTATGGTCCGGACCCTCGAATCAATGATGAAAACATCCAGC
AGGAGAACAGCAACGACGACATGGCGATGGGGATCCCCGGATCACTAAGCAATCAGCCCATCCCTCAATCTCAGGCGGGT
CAAACGCCAGCCGCCGTCAGCCGCAGCCAGGCGCAGCGTAAATATATTTATGATCGCAATATCCGCCATGTGCGTTATCC
GGGTTACAAGCTGGAGAAAATGACCGTTGCGGTGGTGCTGAATAAATCGCTCCCGGTGTTGGAACAGTGGACGCCAGAAC
AGCAGGAAGAACTCAAACGTTTAATTGAAGATGCCGCAGGTATTGATGTTAAACGTGGGGATTCTCTGACCATTAACGTG
ATGGCCTTCGCGGTCCCTACGTTGATTGACGAGCCGGTGATGCCGTGGTGGCAAGAACCAAGTACCTTCCGTTGGGCAGA
ATTGCTGGGGATCGGGCTGCTGTCATTACTGGTGCTGTGGTTTGGTGTTCGCCCATTGATGAAACGCTATTCGCGTAAGG
GTTCGGAGAACCTGCCGTTAGCCATTTCCTCGGCGAGTGCAGATGAGGCGCTTGATCATGTGGATACCGGTGTAGACGGT
GTAGAAAGTAGCCCGCGTGCAGAAACCGCCTTCTCCGCGTCTTCTCTCTGGGAAAGCGACGATCTACCAGAGCAAGGCTC
CGGTTTGGAAACCAAAATCGCCCATCTGCAACAATTGGCGCAGTCAGAAACTGAACGTACCGCCGAAGTCATTAAACAAT
GGATTAACAGTAATGAACGAATCAAATCTCAACCTGAATGA

Upstream 100 bases:

>100_bases
CCAGAAAGCCAGTGTGGCTTTCTCCGCCATGGTGCAGGTGCGCAATAAGCTCACTTCTGCATTAGACGATGTTATGAATA
CTGCGCTGTAGGGTAAATCG

Downstream 100 bases:

>100_bases
TGACTCAAGCGTCAAAAGTGATACCAAGGGGCGCAGCCGATTGGAACAGGCCAGTATTTTATTACTGAGTATTGGTGAAG
AAGCGGCCGCCATGGTGATG

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 546; Mature: 546

Protein sequence:

>546_residues
MLDKIKQKLSPGQLGENTKTILMSAAALLVTAAIIFSLWRSSQGYTALFGSQENIPVTQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNRSDNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAERYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINV
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
VESSPRAETAFSASSLWESDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE

Sequences:

>Translated_546_residues
MLDKIKQKLSPGQLGENTKTILMSAAALLVTAAIIFSLWRSSQGYTALFGSQENIPVTQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNRSDNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAERYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINV
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
VESSPRAETAFSASSLWESDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE
>Mature_546_residues
MLDKIKQKLSPGQLGENTKTILMSAAALLVTAAIIFSLWRSSQGYTALFGSQENIPVTQVVEVLEGEAIAYRINPDNGQV
LVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQFIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSF
AISNRSDNSASVVLRLRYGQTLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAERYGPDPRINDENIQQENSNDDMAMGIPGSLSNQPIPQSQAG
QTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVLNKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINV
MAFAVPTLIDEPVMPWWQEPSTFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
VESSPRAETAFSASSLWESDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNERIKSQPE

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=440, Percent_Identity=31.5909090909091, Blast_Score=183, Evalue=2e-47,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 59913; Mature: 59913

Theoretical pI: Translated: 4.88; Mature: 4.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDKIKQKLSPGQLGENTKTILMSAAALLVTAAIIFSLWRSSQGYTALFGSQENIPVTQV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
VEVLEGEAIAYRINPDNGQVLVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQ
HHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEECCCCHHHHCHHHHCCHHH
FIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSFAISNRSDNSASVVLRLRYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCEEEEEEEECCC
TLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAERYGPDPRINDENIQQENSNDD
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MAMGIPGSLSNQPIPQSQAGQTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHEEEEEEE
NKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINVMAFAVPTLIDEPVMPWWQEP
CCCCCHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHCCCCCCCCCCC
STFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCC
VESSPRAETAFSASSLWESDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNER
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKSQPE
CCCCCC
>Mature Secondary Structure
MLDKIKQKLSPGQLGENTKTILMSAAALLVTAAIIFSLWRSSQGYTALFGSQENIPVTQV
CCHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCCCHHHH
VEVLEGEAIAYRINPDNGQVLVAENQLGKARILLAAKGITATLPIGYELMDKESMLGSSQ
HHHHCCCEEEEEECCCCCEEEEEECCCCCEEEEEEECCCEEECCCCHHHHCHHHHCCHHH
FIQNVRYKRSLEGELAQSMMALSAVEYARVHLGMSEASSFAISNRSDNSASVVLRLRYGQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHEEECCCCCCCEEEEEEEECCC
TLSTEQVGAIVQLVAGSIPGMKPANVRVVDQHGELLSQAYQANSEGVPSVKSGTELAHYL
CCCHHHHHHHHHHHHCCCCCCCCCCEEEECHHHHHHHHHHHCCCCCCCCCCCHHHHHHHH
QSTTEKNIANLLNSVIGANNYRISVSTQLDMSRIEETAERYGPDPRINDENIQQENSNDD
HHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
MAMGIPGSLSNQPIPQSQAGQTPAAVSRSQAQRKYIYDRNIRHVRYPGYKLEKMTVAVVL
EEECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEEEHHEEEEEEE
NKSLPVLEQWTPEQQEELKRLIEDAAGIDVKRGDSLTINVMAFAVPTLIDEPVMPWWQEP
CCCCCHHHHCCCHHHHHHHHHHHHHCCCEEECCCCEEEEHHHHHHHHHHCCCCCCCCCCC
STFRWAELLGIGLLSLLVLWFGVRPLMKRYSRKGSENLPLAISSASADEALDHVDTGVDG
CCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCCCCCCCEEEECCCHHHHHHHHHCCCCC
VESSPRAETAFSASSLWESDDLPEQGSGLETKIAHLQQLAQSETERTAEVIKQWINSNER
CCCCCCHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
IKSQPE
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]