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The map label for this gene is purA [H]

Identifier: 153947819

GI number: 153947819

Start: 4112364

End: 4113662

Strand: Reverse

Name: purA [H]

Synonym: YpsIP31758_3648

Alternate gene names: 153947819

Gene position: 4113662-4112364 (Counterclockwise)

Preceding gene: 153949272

Following gene: 153947883

Centisome position: 87.09

GC content: 50.89

Gene sequence:

>1299_bases
ATGGGTAAGAACGTCGTCGTACTGGGCACCCAATGGGGTGACGAAGGTAAGGGCAAGGTCGTTGACCTGCTGACTGAACG
GGCTAAATATGTTGTGCGCTATCAAGGTGGCCATAACGCGGGCCATACTTTGGTTATCAACGGTGAGAAAACCGTTCTTC
ATTTAATTCCCTCAGGTATTCTACGTGAAAACGTAATCAGCATCATCGGCAACGGTGTTGTACTGGCTCCTGACGCGTTG
ATGAAAGAGATGACAGAGCTTGAAGCGCGCGGTGTGCCTGTTCGTGAGCGACTGTTACTCTCCGAAGCATGCCCATTGAT
CCTGCCTTATCATGTGGCTCTGGATAATGCGCGTGAAAAAGCACGTGGTGCAAAAGCTATCGGTACTACCGGTCGTGGTA
TTGGCCCTGCCTATGAAGATAAAGTTGCTCGCCGCGGCCTACGCGTCAGTGACTTGTTCAATAAAGAAACCTTCGCTATC
AAGCTGAAAGAAATTGTGGAATACCACAACTTCCAGTTGGTGCATTACTACAAAGAAGCGGCGGTTGACTACCAAAAAGT
GCTGGATGATGTATTGGCTATCGCCGATATTCTGACGGCCATGGTAGTTGATGTTTCTGAATTGTTGGACAATGCCCGTA
AGCAAGGCGAGTTGATCATGTTCGAAGGCGCACAAGGCACCTTGCTGGACATCGACCACGGTACTTATCCGTATGTCACC
TCATCTAACACCACCGCGGGTGGCGTAGCGACAGGCTCTGGCCTGGGTCCACGTTATGTTGATTACGTGCTGGGTATCGT
AAAAGCCTATTCTACCCGTGTAGGTGCAGGTCCATTCCCTACCGAACTGAACGATGAAACTGGTGAGTTCCTGCGCAAGC
AAGGTAACGAATATGGCGCGACTACCGGCCGTAGCCGTCGTACTGGCTGGTTGGATATCGTAGCCGTTCGCCGTGCAGTG
CAGATCAACTCCCTGTCTGGTTTCTGCATGACCAAACTGGATGTGCTGGATGGTCTGAAAGAAGTGAAGCTCTGTATTGG
CTACCGTATGCCTGATGGCCGCGAAGTTGATACAACGCCACTGGCTGCAGAAGGCTGGGAAGGCATCGAGCCAATCTACG
AAACCATGCCAGGTTGGTCAGAAACCACCTTTGGTGTGAAGGAACACAGTAAGTTGCCACAGGCAGCCCTGAACTACATT
CAACGTGTAGAAGAGCTGACAGGTGTGCCGATTGATATCATCTCTACCGGCCCTGATCGTGACGAAACCATGATCCTGCG
TGATCCATTTGATGCATAA

Upstream 100 bases:

>100_bases
AAGGTAAGCGCAAAAATTGTTCAGTTATGCGTTAAAAAGTACTGAAAGCCGCCGTATGAGATGTTAGAATCCTTTTTTAA
GCAACCTGGTGATTTTTGAG

Downstream 100 bases:

>100_bases
TTAACTGGCTTACGGCCAAGTTATCATCATTAATGAGATATCAGCATTAAAATGTACGGGGTTAACGCGTATATTTGTGT
TCGAAACAATAAACCGGGCT

Product: adenylosuccinate synthetase

Products: NA

Alternate protein names: AMPSase; AdSS; IMP--aspartate ligase [H]

Number of amino acids: Translated: 432; Mature: 431

Protein sequence:

>432_residues
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDAL
MKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAI
KLKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAV
QINSLSGFCMTKLDVLDGLKEVKLCIGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYI
QRVEELTGVPIDIISTGPDRDETMILRDPFDA

Sequences:

>Translated_432_residues
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDAL
MKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAI
KLKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAV
QINSLSGFCMTKLDVLDGLKEVKLCIGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYI
QRVEELTGVPIDIISTGPDRDETMILRDPFDA
>Mature_431_residues
GKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGILRENVISIIGNGVVLAPDALM
KEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREKARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIK
LKEIVEYHNFQLVHYYKEAAVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVTS
SNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGATTGRSRRTGWLDIVAVRRAVQ
INSLSGFCMTKLDVLDGLKEVKLCIGYRMPDGREVDTTPLAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQ
RVEELTGVPIDIISTGPDRDETMILRDPFDA

Specific function: Plays an important role in the de novo pathway of purine nucleotide biosynthesis. Catalyzes the first commited step in the biosynthesis of AMP from IMP [H]

COG id: COG0104

COG function: function code F; Adenylosuccinate synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the adenylosuccinate synthetase family [H]

Homologues:

Organism=Homo sapiens, GI22748717, Length=426, Percent_Identity=43.1924882629108, Blast_Score=328, Evalue=7e-90,
Organism=Homo sapiens, GI34577063, Length=428, Percent_Identity=41.588785046729, Blast_Score=315, Evalue=7e-86,
Organism=Homo sapiens, GI40316944, Length=400, Percent_Identity=41.25, Blast_Score=287, Evalue=1e-77,
Organism=Escherichia coli, GI1790620, Length=432, Percent_Identity=88.4259259259259, Blast_Score=793, Evalue=0.0,
Organism=Caenorhabditis elegans, GI25146553, Length=429, Percent_Identity=42.6573426573427, Blast_Score=334, Evalue=5e-92,
Organism=Caenorhabditis elegans, GI25146555, Length=429, Percent_Identity=42.6573426573427, Blast_Score=334, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6324109, Length=434, Percent_Identity=41.9354838709677, Blast_Score=338, Evalue=9e-94,
Organism=Drosophila melanogaster, GI21356033, Length=421, Percent_Identity=42.5178147268409, Blast_Score=340, Evalue=8e-94,

Paralogues:

None

Copy number: 940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018220
- InterPro:   IPR001114 [H]

Pfam domain/function: PF00709 Adenylsucc_synt [H]

EC number: =6.3.4.4 [H]

Molecular weight: Translated: 47293; Mature: 47161

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: PS01266 ADENYLOSUCCIN_SYN_1 ; PS00513 ADENYLOSUCCIN_SYN_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGI
CCCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRENVISIIGNGVVLAPDALMKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREK
HHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIKLKEIVEYHNFQLVHYYKEA
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCHHEEEHHHHHHHHCEEEEEHHHHH
AVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
TTGRSRRTGWLDIVAVRRAVQINSLSGFCMTKLDVLDGLKEVKLCIGYRMPDGREVDTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQRVEELTGVPIDIISTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
DETMILRDPFDA
CCEEEECCCCCC
>Mature Secondary Structure 
GKNVVVLGTQWGDEGKGKVVDLLTERAKYVVRYQGGHNAGHTLVINGEKTVLHLIPSGI
CCCEEEEEECCCCCCCCHHHHHHHHHHHEEEEECCCCCCCCEEEECCCCEEEEECCCHH
LRENVISIIGNGVVLAPDALMKEMTELEARGVPVRERLLLSEACPLILPYHVALDNAREK
HHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCHHHHHHHHHCCCEEEEHHHHHHHHHHH
ARGAKAIGTTGRGIGPAYEDKVARRGLRVSDLFNKETFAIKLKEIVEYHNFQLVHYYKEA
HCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHCCCCHHEEEHHHHHHHHCEEEEEHHHHH
AVDYQKVLDDVLAIADILTAMVVDVSELLDNARKQGELIMFEGAQGTLLDIDHGTYPYVT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCEEECCCCCCCEEE
SSNTTAGGVATGSGLGPRYVDYVLGIVKAYSTRVGAGPFPTELNDETGEFLRKQGNEYGA
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCC
TTGRSRRTGWLDIVAVRRAVQINSLSGFCMTKLDVLDGLKEVKLCIGYRMPDGREVDTTP
CCCCCCCCCHHHHHHHHHHHEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
LAAEGWEGIEPIYETMPGWSETTFGVKEHSKLPQAALNYIQRVEELTGVPIDIISTGPDR
CCCCCCCCCCHHHHHCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHCCCCEEEEECCCCC
DETMILRDPFDA
CCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA