Click here to switch to the map view.

The map label for this gene is cpdB [H]

Identifier: 153947556

GI number: 153947556

Start: 4097502

End: 4099472

Strand: Direct

Name: cpdB [H]

Synonym: YpsIP31758_3631

Alternate gene names: 153947556

Gene position: 4097502-4099472 (Clockwise)

Preceding gene: 153949120

Following gene: 153947006

Centisome position: 86.75

GC content: 48.5

Gene sequence:

>1971_bases
ATGGAGAAAGCCATGTTCAAGCGTCCGCTGACGTTATCCCTCCTTGCCAACCTGATTGCTGTCGCGACATCACTGTCTGC
TGCCACTGTTGATTTACGAGTATTGGAAACCACCGACCTACACAGCAACATGATGGATTTTGATTACTACAAAGATAAAC
CCACGGAAAAGTTCGGGCTGGTACGCACTGCCAGCTTGATTATCGCAGCCCGGCAACAAGCCACTAACAGCGTGCTGGTG
GATAACGGTGACGTGATCCAGGGGAGTCCGCTTGGTGATTATATCGCGGCTAAAGGGTTAAACGACGGAGAGATTCACCC
AGTCTATAAAGCGATGAACACGCTGGATTATGCGGTCGGCAATATCGGTAACCATGAGTTTAACTATGGGCTGGACTACC
TGAAGAAATCACTGGCAGGGGCGAAATTCCCATATGTTAACGCCAACGTGATTGATGTTAAAACAGGTAAACCCTTGTTC
CAGCCTTACCTGATCATCGATACCCCGGTGAAAGATCGTGACGGAAAGAGCCATAACCTGCGGATCGGGTACATTGGTTT
TGTACCGCCTCAAGTGATGATCTGGGATAAGGCGAATCTGAGTGGCAAAGTCACGGTTAACGATATCACTGAAACTGCCA
AGAAGTGGGTACCGGAGATGCGTGAGCAAGGGGCTGATCTGGTTGTTGCTATCCCGCACTCTGGTCTTTCCAGCGATCCT
TATAAAACCATGGCTGAAAATTCGGTTTATTACCTCAGTCAAGTTCCAGGTATCGATGCCATCATGTTCGGCCACGCACA
TGGTGTGTTCCCCAGCAAAGATTTTGCCGCCATCAAAGGCGCGGATATCACTCAAGGTACCTTAAACGGTATTCCGGCAG
TGATGCCTGGCCAATGGGGTGACCATTTAGGCGTAGTCGATTTTGTCCTGAATAATGATCAGGGAAAATGGCAAGTTATC
GATGCCAAGGCTGAAGCCCGTCCTATCTACGATAAAACAGCACAAAAATCACTGGCGGCAGAAAATGCCAAACTGGTGGA
AGTACTGGCAGTTGATCACCAGAGCACCCGTGATTTTGTCAGCCAACCGATTGGTGAAGCATCAGATAACATGTACAGCT
ATCTGGCACTGATCCAAGATGACCCCACCGTGCAGATTGTGAATAACGCCCAGCGCGCGTATACCGAACATTTTATTCAG
GGCGATCCTGATTTAGCTGACCTCCCGGTACTGTCTGCCGCTGCACCGTTTAAAGCCGGTGGCCGCAAAAATGATCCCAC
CAGTTTTGTTGAGGTGGAAAAAGGGAAGCTGACCTTCCGTAACGCCGCGGATTTGTACCTTTATCCAAATACGCTCGTCG
TCGTGAAAGCCAGTGGGGCGGAGGTTAAGCAGTGGCTGGAGTGTTCCGCCGCACAGTTTAATCAAATCGATATTAACAGC
AGCAAACCGCAATCGTTGATTAATTGGGATGGTTTTCGTACTTATAATTTCGATGTCATCGATGGTGTAGAGTATGAAAT
TGATGTCAGCCAGCCTGCCCGTTACGATGGCGAATGTACGCTAATCAACGACCAAGCTGAACGTATCAAAAACCTGACGT
TTAACGGTAAGCCTATCGATCCGAAAGCCACATTCCTGATTGCCACCAATAACTACCGCGCTTACAGCGGTAAGTTTGCC
GGTACCGGTGACAGCCATATCGCATTCGCTTCACCCGACGAAAATCGAGCGGTCCTGTCTGCATATATCAGTGCTGAAAC
CAAGAAAAAGGGTCAGGTCATCCCGCGAGCCGACAACAATTGGCGTCTGGCAACCCTTGATAGTAAGCAACCTTTGGATA
TTCGTTTTGAAACCTCTCCAACTGCTAAGGCCGCTGATTTTATTAAACAAAAAGCGCAATACCCAATGAAAACACAGGGT
ACGGATGAGGTTGGCTTCGCCGTTTATCAAATCGATTTACAGAGAAAGTAA

Upstream 100 bases:

>100_bases
ACACTTTGCTTTAGCAATTAATTAAAATTACATATTCTTTTGCGATATATATCGAACATATACCAAGTACGAAGGGTAGC
GTTTTTAATGGAAACCCACG

Downstream 100 bases:

>100_bases
TCTCTGGCGGCAGCGCCTTACTGTGCGCTGCCGTTACCCCATAATACCCACTTGGAGTTATATCACAATCTATAACATGT
ATTTATTATGCATGTTATAG

Product: bifunctional 2',3'-cyclic nucleotide 2'-phosphodiesterase/3'-nucleotidase periplasmic precursor protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MEKAMFKRPLTLSLLANLIAVATSLSAATVDLRVLETTDLHSNMMDFDYYKDKPTEKFGLVRTASLIIAARQQATNSVLV
DNGDVIQGSPLGDYIAAKGLNDGEIHPVYKAMNTLDYAVGNIGNHEFNYGLDYLKKSLAGAKFPYVNANVIDVKTGKPLF
QPYLIIDTPVKDRDGKSHNLRIGYIGFVPPQVMIWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDP
YKTMAENSVYYLSQVPGIDAIMFGHAHGVFPSKDFAAIKGADITQGTLNGIPAVMPGQWGDHLGVVDFVLNNDQGKWQVI
DAKAEARPIYDKTAQKSLAAENAKLVEVLAVDHQSTRDFVSQPIGEASDNMYSYLALIQDDPTVQIVNNAQRAYTEHFIQ
GDPDLADLPVLSAAAPFKAGGRKNDPTSFVEVEKGKLTFRNAADLYLYPNTLVVVKASGAEVKQWLECSAAQFNQIDINS
SKPQSLINWDGFRTYNFDVIDGVEYEIDVSQPARYDGECTLINDQAERIKNLTFNGKPIDPKATFLIATNNYRAYSGKFA
GTGDSHIAFASPDENRAVLSAYISAETKKKGQVIPRADNNWRLATLDSKQPLDIRFETSPTAKAADFIKQKAQYPMKTQG
TDEVGFAVYQIDLQRK

Sequences:

>Translated_656_residues
MEKAMFKRPLTLSLLANLIAVATSLSAATVDLRVLETTDLHSNMMDFDYYKDKPTEKFGLVRTASLIIAARQQATNSVLV
DNGDVIQGSPLGDYIAAKGLNDGEIHPVYKAMNTLDYAVGNIGNHEFNYGLDYLKKSLAGAKFPYVNANVIDVKTGKPLF
QPYLIIDTPVKDRDGKSHNLRIGYIGFVPPQVMIWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDP
YKTMAENSVYYLSQVPGIDAIMFGHAHGVFPSKDFAAIKGADITQGTLNGIPAVMPGQWGDHLGVVDFVLNNDQGKWQVI
DAKAEARPIYDKTAQKSLAAENAKLVEVLAVDHQSTRDFVSQPIGEASDNMYSYLALIQDDPTVQIVNNAQRAYTEHFIQ
GDPDLADLPVLSAAAPFKAGGRKNDPTSFVEVEKGKLTFRNAADLYLYPNTLVVVKASGAEVKQWLECSAAQFNQIDINS
SKPQSLINWDGFRTYNFDVIDGVEYEIDVSQPARYDGECTLINDQAERIKNLTFNGKPIDPKATFLIATNNYRAYSGKFA
GTGDSHIAFASPDENRAVLSAYISAETKKKGQVIPRADNNWRLATLDSKQPLDIRFETSPTAKAADFIKQKAQYPMKTQG
TDEVGFAVYQIDLQRK
>Mature_656_residues
MEKAMFKRPLTLSLLANLIAVATSLSAATVDLRVLETTDLHSNMMDFDYYKDKPTEKFGLVRTASLIIAARQQATNSVLV
DNGDVIQGSPLGDYIAAKGLNDGEIHPVYKAMNTLDYAVGNIGNHEFNYGLDYLKKSLAGAKFPYVNANVIDVKTGKPLF
QPYLIIDTPVKDRDGKSHNLRIGYIGFVPPQVMIWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDP
YKTMAENSVYYLSQVPGIDAIMFGHAHGVFPSKDFAAIKGADITQGTLNGIPAVMPGQWGDHLGVVDFVLNNDQGKWQVI
DAKAEARPIYDKTAQKSLAAENAKLVEVLAVDHQSTRDFVSQPIGEASDNMYSYLALIQDDPTVQIVNNAQRAYTEHFIQ
GDPDLADLPVLSAAAPFKAGGRKNDPTSFVEVEKGKLTFRNAADLYLYPNTLVVVKASGAEVKQWLECSAAQFNQIDINS
SKPQSLINWDGFRTYNFDVIDGVEYEIDVSQPARYDGECTLINDQAERIKNLTFNGKPIDPKATFLIATNNYRAYSGKFA
GTGDSHIAFASPDENRAVLSAYISAETKKKGQVIPRADNNWRLATLDSKQPLDIRFETSPTAKAADFIKQKAQYPMKTQG
TDEVGFAVYQIDLQRK

Specific function: This bifunctional enzyme catalyzes two consecutive reactions during ribonucleic acid degradation. Converts a 2',3'- cyclic nucleotide to a 3'-nucleotide and then the 3'-nucleotide to the corresponding nucleoside and phosphate [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 5'-nucleotidase family [H]

Homologues:

Organism=Escherichia coli, GI1790658, Length=646, Percent_Identity=76.0061919504644, Blast_Score=1051, Evalue=0.0,
Organism=Escherichia coli, GI1786687, Length=584, Percent_Identity=23.458904109589, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI24652512, Length=196, Percent_Identity=28.0612244897959, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI161076508, Length=196, Percent_Identity=28.0612244897959, Blast_Score=73, Evalue=6e-13,
Organism=Drosophila melanogaster, GI19921980, Length=196, Percent_Identity=28.0612244897959, Blast_Score=73, Evalue=7e-13,

Paralogues:

None

Copy number: 100 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR006294
- InterPro:   IPR004843 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00149 Metallophos [H]

EC number: =3.1.3.6; =3.1.4.16 [H]

Molecular weight: Translated: 72160; Mature: 72160

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: PS00785 5_NUCLEOTIDASE_1 ; PS00786 5_NUCLEOTIDASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKAMFKRPLTLSLLANLIAVATSLSAATVDLRVLETTDLHSNMMDFDYYKDKPTEKFGL
CCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
VRTASLIIAARQQATNSVLVDNGDVIQGSPLGDYIAAKGLNDGEIHPVYKAMNTLDYAVG
HHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHC
NIGNHEFNYGLDYLKKSLAGAKFPYVNANVIDVKTGKPLFQPYLIIDTPVKDRDGKSHNL
CCCCCCCCCCHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEE
RIGYIGFVPPQVMIWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDP
EEEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
YKTMAENSVYYLSQVPGIDAIMFGHAHGVFPSKDFAAIKGADITQGTLNGIPAVMPGQWG
CHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCCC
DHLGVVDFVLNNDQGKWQVIDAKAEARPIYDKTAQKSLAAENAKLVEVLAVDHQSTRDFV
CCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHH
SQPIGEASDNMYSYLALIQDDPTVQIVNNAQRAYTEHFIQGDPDLADLPVLSAAAPFKAG
HCCCCCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCC
GRKNDPTSFVEVEKGKLTFRNAADLYLYPNTLVVVKASGAEVKQWLECSAAQFNQIDINS
CCCCCCCCEEEEECCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCEEECCC
SKPQSLINWDGFRTYNFDVIDGVEYEIDVSQPARYDGECTLINDQAERIKNLTFNGKPID
CCCCHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCEEEEECCHHHHHHCCCCCCCCCC
PKATFLIATNNYRAYSGKFAGTGDSHIAFASPDENRAVLSAYISAETKKKGQVIPRADNN
CCEEEEEEECCCEEECCCCCCCCCCEEEEECCCCCCEEEEEEHHHCCCCCCCCCCCCCCC
WRLATLDSKQPLDIRFETSPTAKAADFIKQKAQYPMKTQGTDEVGFAVYQIDLQRK
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECC
>Mature Secondary Structure
MEKAMFKRPLTLSLLANLIAVATSLSAATVDLRVLETTDLHSNMMDFDYYKDKPTEKFGL
CCCHHHCCCCHHHHHHHHHHHHHCCCEEEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHH
VRTASLIIAARQQATNSVLVDNGDVIQGSPLGDYIAAKGLNDGEIHPVYKAMNTLDYAVG
HHHHHHHHHHHHHCCCEEEECCCCEEECCCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHC
NIGNHEFNYGLDYLKKSLAGAKFPYVNANVIDVKTGKPLFQPYLIIDTPVKDRDGKSHNL
CCCCCCCCCCHHHHHHHHCCCCCCEECCEEEEEECCCCCCCCEEEEECCCCCCCCCCCEE
RIGYIGFVPPQVMIWDKANLSGKVTVNDITETAKKWVPEMREQGADLVVAIPHSGLSSDP
EEEEEECCCCEEEEEECCCCCCEEEEHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCC
YKTMAENSVYYLSQVPGIDAIMFGHAHGVFPSKDFAAIKGADITQGTLNGIPAVMPGQWG
CHHHHCCCEEEEECCCCCEEEEEECCCCCCCCCCCCEECCCCCCCCHHCCCCCCCCCCCC
DHLGVVDFVLNNDQGKWQVIDAKAEARPIYDKTAQKSLAAENAKLVEVLAVDHQSTRDFV
CCCCEEEEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHHCCCEEEEEEEECCCHHHHHH
SQPIGEASDNMYSYLALIQDDPTVQIVNNAQRAYTEHFIQGDPDLADLPVLSAAAPFKAG
HCCCCCCCCCCEEEEEEECCCCEEEEECCHHHHHHHHHHCCCCCCCCCCHHHCCCCCCCC
GRKNDPTSFVEVEKGKLTFRNAADLYLYPNTLVVVKASGAEVKQWLECSAAQFNQIDINS
CCCCCCCCEEEEECCEEEEECCCEEEECCCEEEEEEECCHHHHHHHHHCCCCCCEEECCC
SKPQSLINWDGFRTYNFDVIDGVEYEIDVSQPARYDGECTLINDQAERIKNLTFNGKPID
CCCCHHCCCCCEEEEEEEEECCEEEEEECCCCCCCCCEEEEECCHHHHHHCCCCCCCCCC
PKATFLIATNNYRAYSGKFAGTGDSHIAFASPDENRAVLSAYISAETKKKGQVIPRADNN
CCEEEEEEECCCEEECCCCCCCCCCEEEEECCCCCCEEEEEEHHHCCCCCCCCCCCCCCC
WRLATLDSKQPLDIRFETSPTAKAADFIKQKAQYPMKTQGTDEVGFAVYQIDLQRK
EEEEEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCEEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA