Click here to switch to the map view.

The map label for this gene is celF [H]

Identifier: 153947458

GI number: 153947458

Start: 4429006

End: 4430319

Strand: Direct

Name: celF [H]

Synonym: YpsIP31758_3951

Alternate gene names: 153947458

Gene position: 4429006-4430319 (Clockwise)

Preceding gene: 153948212

Following gene: 153946844

Centisome position: 93.77

GC content: 50.53

Gene sequence:

>1314_bases
ATGAGCACGTTTAAAATAACCATTATTGGCGGTGGGAGTAGCTATACCCCAGAGCTAGTTGATGGGTTGATTCAGCGTAT
TGAACAACTGCCCGTCACTGAACTGGCACTGGTTGATGTCGAGCAGGGCCGCCAAAAGGTGGAAATTATTGCCGCGCTGA
CCCAAAGAATGCTTGCCCGTCATGGGTTGGAACAGGTCAAAGTCAGTGTCCACTACTCGCTGGATGAAGCCATTCGCGGT
GCCCGTTTTGTCCTGACACAATTTCGTGTCGGCCAACTCCCTGCCAGGGCAGCAGACGAACGCTTGGGGTTAAAATATAA
CCTGCTCGGTCAGGAGACGACCGGAATTGGTGGCTTTGCCAAAGCGCTGCGTACCATTCCGGTGATGCTTGATATTGCTG
CTAACGTTGAGCGATTGGCCCCCGATGCCTGGATCATTAACTTCACTAACCCAGCCGGCATTGTCACCGAAGCCGTTAGC
CGCTATACCAAAGCGAAAATCATTGGTCTGTGTAATGTCCCGATCAGTATGCATCATATGATTGCCAAGCTCTTGCAAGC
CCCCTACGAAGACCTCCAACTGCGCTTTGCCGGGCTGAATCATATGGTATGGGTACATGAAGTATTACAACGCGGGAAAG
ATGTCACCGCTGATGTGCTGAAAATGCTGTGTGATGGGGCATCATTAAGCATGAACAATATTAAGGAAGCCCCATGGCCA
CCCGAGTTCCTGCAAGCAATGGGGGCCATCCCCTGCCCATACCATCGCTATTTCTACCAAACGCAAGACATGCTGGCAGA
AGAGATGGCCGCAGCAGCTGAGCGTGGTACCCGTGCGGAACAGGTGATGCAGGTGGAAAAAGAGCTGTTCGCGCTGTACG
CCGATCCGCATTTGGATACCAAACCAGAGCAGCTCAGCTTCCGGGGTGGATCATTTTATTCTGAAGTTGCATTGGAACTT
ATTCGTGCCATCCATAACAACTTAGGGACGCAATTAGTAGTAAATACAACAAACCGTGGCGCGATTCGCGGTTTATCTGA
TGATTCTGTGGTAGAAACTAACTGTATTATTGATGCAAAAGGGGCTCATCCATTAACCTTCGGCCCTTTACCGGTCTCTA
TGCATGGGCTAACCCAACAGGTAAAAGCCTATGAACGATTGACGATTGACGCGGCGGTACATGGCGATCGTCGCAGTGCC
TTGTTGGCCTTAGTGACAAATCCACTGATTGCTAACGCCAGCATTGCTCAACCGTTACTGGAGGAGGTACTACAGGTGAA
TAAACCTTACCTGCCTCAGTTCTCCGGTTTGTGA

Upstream 100 bases:

>100_bases
TATCCACAGATATGTTATTCACAAACATGTTACCCACAGACATGTTATCCACAGACATGACTGAACTTTATCGCCGGCAG
AGCCGTTTGGAGCCCCTACA

Downstream 100 bases:

>100_bases
TGACCTAGCCAACGTCATTGGTGTTGTAGCACTATCAGTTCGGTTTATAACGACATCAGCTTGTTTGTAGCGACATTGGT
TTTTTTTGTAACAATATCAG

Product: 6-phospho-beta-glucosidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 437; Mature: 436

Protein sequence:

>437_residues
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRG
ARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVS
RYTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKDVTADVLKMLCDGASLSMNNIKEAPWP
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALEL
IRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSA
LLALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL

Sequences:

>Translated_437_residues
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRG
ARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVS
RYTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKDVTADVLKMLCDGASLSMNNIKEAPWP
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALEL
IRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSA
LLALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL
>Mature_436_residues
STFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLARHGLEQVKVSVHYSLDEAIRGA
RFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFAKALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSR
YTKAKIIGLCNVPISMHHMIAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKDVTADVLKMLCDGASLSMNNIKEAPWPP
EFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDTKPEQLSFRGGSFYSEVALELI
RAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAKGAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSAL
LALVTNPLIANASIAQPLLEEVLQVNKPYLPQFSGL

Specific function: Hydrolyzes phospho-beta-glucosides (Probable) [H]

COG id: COG1486

COG function: function code G; Alpha-galactosidases/6-phospho-beta-glucosidases, family 4 of glycosyl hydrolases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 4 family [H]

Homologues:

Organism=Escherichia coli, GI1788029, Length=440, Percent_Identity=43.1818181818182, Blast_Score=345, Evalue=2e-96,
Organism=Escherichia coli, GI1790560, Length=440, Percent_Identity=26.3636363636364, Blast_Score=115, Evalue=7e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019802
- InterPro:   IPR001088
- InterPro:   IPR022616
- InterPro:   IPR015955
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02056 Glyco_hydro_4; PF11975 Glyco_hydro_4C [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 48106; Mature: 47975

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01324 GLYCOSYL_HYDROL_F4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLAR
CCEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHH
HGLEQVKVSVHYSLDEAIRGARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFA
CCHHHEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCHHHH
KALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSRYTKAKIIGLCNVPISMHHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
IAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKDVTADVLKMLCDGASLSMNNIKEAPWP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGSFYSEVALELIRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAK
CCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECEEEECC
GAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSALLALVTNPLIANASIAQPLL
CCCCCEECCCCCCHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHCCHHCCCHHHHHHH
EEVLQVNKPYLPQFSGL
HHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
STFKITIIGGGSSYTPELVDGLIQRIEQLPVTELALVDVEQGRQKVEIIAALTQRMLAR
CEEEEEEEECCCCCCHHHHHHHHHHHHHCCCHHEEEEEHHHCHHHHHHHHHHHHHHHHH
HGLEQVKVSVHYSLDEAIRGARFVLTQFRVGQLPARAADERLGLKYNLLGQETTGIGGFA
CCHHHEEEEEEECHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCEEEECCCCCCCCHHHH
KALRTIPVMLDIAANVERLAPDAWIINFTNPAGIVTEAVSRYTKAKIIGLCNVPISMHHM
HHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHEEEEECCCHHHHHH
IAKLLQAPYEDLQLRFAGLNHMVWVHEVLQRGKDVTADVLKMLCDGASLSMNNIKEAPWP
HHHHHHCCHHHHHHHHHCCCHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCCCCCCCCC
PEFLQAMGAIPCPYHRYFYQTQDMLAEEMAAAAERGTRAEQVMQVEKELFALYADPHLDT
HHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCC
KPEQLSFRGGSFYSEVALELIRAIHNNLGTQLVVNTTNRGAIRGLSDDSVVETNCIIDAK
CCCCEECCCCCHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCEEEECEEEECC
GAHPLTFGPLPVSMHGLTQQVKAYERLTIDAAVHGDRRSALLALVTNPLIANASIAQPLL
CCCCCEECCCCCCHHHHHHHHHHHHHHEEEHHCCCCHHHHHHHHHHCCHHCCCHHHHHHH
EEVLQVNKPYLPQFSGL
HHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8990303; 9384377; 8969509 [H]