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The map label for this gene is tilS [H]

Identifier: 153947345

GI number: 153947345

Start: 1236257

End: 1237612

Strand: Direct

Name: tilS [H]

Synonym: YpsIP31758_1031

Alternate gene names: 153947345

Gene position: 1236257-1237612 (Clockwise)

Preceding gene: 153949033

Following gene: 153947172

Centisome position: 26.17

GC content: 48.53

Gene sequence:

>1356_bases
GTGTTACTTAACCCTCTTTTCGCTCAACTGGGTGAAAACCGTCATGTGTTGGTGGGGTTTAGTGGTGGGTTAGATTCCAC
GGTGTTACTGCATTTATTGGTCTGTTTACGTCAACAACTTATTCCTGAACTGAACATTCGCGCTATTCATATTCACCATG
GTTTAAACCCGCAGGCTGATAGTTGGGTAAAACATTGTATGCAGCAATGTGATCAATGGAAAATTGAGCTTAAGGTGGTG
CGAGTTAATATTGATCCTCGGCAGAACGGGATAGAAGCGGCAGCCAGAACAGCACGTTACCAAGCCTTTTCCGCCAATTT
GGCTGCCAAAGAAGTCTTACTGACCGCCCAGCATTTGGACGATCAATGTGAGACCTTCCTGTTGGCACTTAAGCGTGGTA
GCGGGCCGGCGGGTCTCTCTGCGATGGCGGCTAAAATGCCCTTTGCCCACAGCCAACTGTTGCGGCCACTACTCGCTTTT
TCTCGAGAGATATTAGAGAACTATGCACAGGCACAGCAATTACAATGGATTGAAGATGATAGCAATCAAGATGATCGTTT
TGATCGCAATTTCTTACGGCTAAATGTTCTGCCGATACTTAATCAACGTTGGCCACATTTTGCTCAGGCCACGGCACGTA
GTGCGGGGCTATGTGCTGAGCAGGAGCAGTTATTGGATGAACTGTTGGCTGAAAACTTGCAGCAGTTACAGGGACCTGAC
AGATCACTCTCTATTGATGGACTATTACAGGCATCAATGGCGAAACGGGCGGCTATATTGCGCCGCTGGTTGGCGAGTCT
TGGTGCACCAATGCCATCACAAAGCCAATTGCAGCGCCTGTGGCTGGAGGTCGCCATGGCACGGCAAGATGCGGAGCCTC
AATTAATGATTGGCACCCGTCAGGTACGCCGTTTTCGTCAACATCTGTATTTATTAATGCCCTTGGCTGAAATAACAACA
AATTACTTACCTTGGGCAACCGTTAAGGCCACCCCAAATTCGTCGATAATACCTTTATTACCCGAGCCATTATGGCTACC
TGCCGATTTAGGCGTACTGAGGTTTGTCTCAGCGGGTGGGCAAGCGGTCCGTCCTGCCACCGTTGGCGAGGAGATAAGTG
TACGTTTTGGTTTACAGGGAGATATCAAAATTGTTGGGCGGCATCACTCGCGTCAAAGTAAAAAAGTATGGCAAGAGCTG
GGTATTCCCCCATGGCAGCGGGAACGTATTCCGCTGTTATATTTCGGTGAACAATTAATTGCAGCCGCGGGGGTATTCGT
TACCCAAGCGGGGCAGGCGAATGAAAATGAGCCATGCTGGCACTTAGATTGGGATAAGCAACTTAAATTGGGATAA

Upstream 100 bases:

>100_bases
CAATCACGTTTTACTTTCTTTAATGACCCAGATGGTTTACCACTGGAGCTATATGAATTGAAAGCGGAATGACATGAACT
TAGTCACCTCGAAACCCAAT

Downstream 100 bases:

>100_bases
ACCACTTAGATTGGGATAAACCAACACCCGCATTGAAAAAGAAGAAGAGAGAGTCGAGGTTGATGACTCAATGCCCACAC
CGGAGAGAATAAATAGATCG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCDQWKIELKVV
RVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAF
SREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAENLQQLQGPD
RSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYLLMPLAEITT
NYLPWATVKATPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPATVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQEL
GIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKQLKLG

Sequences:

>Translated_451_residues
MLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCDQWKIELKVV
RVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAF
SREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAENLQQLQGPD
RSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYLLMPLAEITT
NYLPWATVKATPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPATVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQEL
GIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKQLKLG
>Mature_451_residues
MLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQADSWVKHCMQQCDQWKIELKVV
RVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLDDQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAF
SREILENYAQAQQLQWIEDDSNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAENLQQLQGPD
RSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTRQVRRFRQHLYLLMPLAEITT
NYLPWATVKATPNSSIIPLLPEPLWLPADLGVLRFVSAGGQAVRPATVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQEL
GIPPWQRERIPLLYFGEQLIAAAGVFVTQAGQANENEPCWHLDWDKQLKLG

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=441, Percent_Identity=55.1020408163265, Blast_Score=430, Evalue=1e-121,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR015262
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF09179 DUF1946; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 50785; Mature: 50785

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQAD
CCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
SWVKHCMQQCDQWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLD
HHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAFSREILENYAQAQQLQWIEDD
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAENLQQLQGPD
CCCCCHHCCCEEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
RSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECHH
QVRRFRQHLYLLMPLAEITTNYLPWATVKATPNSSIIPLLPEPLWLPADLGVLRFVSAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCC
QAVRPATVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQELGIPPWQRERIPLLYFGEQLI
CEECCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHH
AAAGVFVTQAGQANENEPCWHLDWDKQLKLG
HHHHHHEEECCCCCCCCCCEECCCCCCCCCC
>Mature Secondary Structure
MLLNPLFAQLGENRHVLVGFSGGLDSTVLLHLLVCLRQQLIPELNIRAIHIHHGLNPQAD
CCCCHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCHH
SWVKHCMQQCDQWKIELKVVRVNIDPRQNGIEAAARTARYQAFSANLAAKEVLLTAQHLD
HHHHHHHHHCCCEEEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DQCETFLLALKRGSGPAGLSAMAAKMPFAHSQLLRPLLAFSREILENYAQAQQLQWIEDD
HHHHHHHHHHHCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SNQDDRFDRNFLRLNVLPILNQRWPHFAQATARSAGLCAEQEQLLDELLAENLQQLQGPD
CCCCCHHCCCEEEEEEEHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCC
RSLSIDGLLQASMAKRAAILRRWLASLGAPMPSQSQLQRLWLEVAMARQDAEPQLMIGTR
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCEEECHH
QVRRFRQHLYLLMPLAEITTNYLPWATVKATPNSSIIPLLPEPLWLPADLGVLRFVSAGG
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHCCC
QAVRPATVGEEISVRFGLQGDIKIVGRHHSRQSKKVWQELGIPPWQRERIPLLYFGEQLI
CEECCCCCCCCEEEEECCCCCEEEEECCCCHHHHHHHHHCCCCCCCCCCCCCEEECHHHH
AAAGVFVTQAGQANENEPCWHLDWDKQLKLG
HHHHHHEEECCCCCCCCCCEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430 [H]