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The map label for this gene is rluB

Identifier: 153947333

GI number: 153947333

Start: 2232703

End: 2233659

Strand: Reverse

Name: rluB

Synonym: YpsIP31758_1928

Alternate gene names: 153947333

Gene position: 2233659-2232703 (Counterclockwise)

Preceding gene: 153949288

Following gene: 153948350

Centisome position: 47.29

GC content: 51.41

Gene sequence:

>957_bases
GTGGGTGAAAAATTACAGAAAATCCTGGCGCGCGCTGGCCATGGTTCTCGTCGTGAAATTGAAGCCATTATCCAGCAAGG
CCGCGTCAGTGTTGACGGTAAAGTGTCAAAGCTAGGTGATCGTGTAGAAGTGACCCAGTCCACTAAAATTCGTCTGGATG
GTCATCTTCTTTCAATTAAAGAATCTGAAGAAAATGTGTGCCGCGTTCTGGCGTATTACAAGCCAGAAGGTGAGCTTTGT
ACGCGTAATGATCCGGAAGGTCGTCCAACGGTATTTGACCGTTTACCTAAACTGCGTGGTTCACGTTGGGTTGCGGTTGG
TCGTTTGGATGTGAACACCTCTGGTTTATTACTGTTCACTACCGATGGCGAATTGGCTAACCGCCTGATGCACCCAAGCC
GTGAAGTTGAGCGTGAATATGCGGTGCGGGTATTTGGTCAAATCGATGATGATAAAATCAAACAGTTGAGCCGTGGTGTA
CAACTGGAAGATGGCCCTGCGGCATTTCGTACTATCAGCTATCAGGGTGGTGAAGGGATAAACCAGTGGTATAACGTTAC
GTTGACCGAAGGGCGCAACCGCGAAGTTCGCCGTCTATGGGAAGCCGTAGGCGTGCAGGTTAGCCGCCTGATTCGTGTGC
GTTATGGCGACATTAATTTGCCGAAAGGCCTGCCACGGGGTGGCTGGACTGAACTGGATCTTAAAGCCACTAACTACCTG
CGTGAATTGGTCGAACTGGATGTTGAAACCGTCAGTAAATTACCCGTTGAGAAAGATCGTCGCCGGGTTAAAGCCAACCA
AATCCGTCGTGCAGTTAAGCGCCATACCGAAGTCTCGGGCCGCCAGGTTGCTGGCCGTCAGGGTTCAGCCCGTAAAGGCT
CTACGCGTCAAAACGTGGGTAATGCGGCACCCGCAGCCACGGCAAGTCGCCGTAGTGGCCCGAAAAAACGCGGTTAA

Upstream 100 bases:

>100_bases
AGGCGACATTAGAGGTTGCTCAATGAGCGAGAAGTCAGAAAAGCAAAAGTTACCAACCCCAAATCCACACACCCCTGCTA
AGCCACAGGGCGCTAAAATT

Downstream 100 bases:

>100_bases
TCTCAGGCGGATTAATGCGTCGTTAACCGCGTCTGTCATTAGACATCAATAGCGACGTGAATAATGCTTATTGCCGCAGT
TTTTTGGACTGCGGCAATAT

Product: 23S rRNA pseudouridylate synthase B

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B

Number of amino acids: Translated: 318; Mature: 317

Protein sequence:

>318_residues
MGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIKESEENVCRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGV
QLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYL
RELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGSTRQNVGNAAPAATASRRSGPKKRG

Sequences:

>Translated_318_residues
MGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIKESEENVCRVLAYYKPEGELC
TRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGV
QLEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYL
RELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGSTRQNVGNAAPAATASRRSGPKKRG
>Mature_317_residues
GEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIKESEENVCRVLAYYKPEGELCT
RNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFTTDGELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGVQ
LEDGPAAFRTISYQGGEGINQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYLR
ELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGSTRQNVGNAAPAATASRRSGPKKRG

Specific function: Responsible for synthesis of pseudouridine from uracil- 2605 in 23S ribosomal RNA

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain

Homologues:

Organism=Escherichia coli, GI1787524, Length=291, Percent_Identity=83.5051546391753, Blast_Score=499, Evalue=1e-142,
Organism=Escherichia coli, GI1790453, Length=253, Percent_Identity=30.4347826086957, Blast_Score=104, Evalue=8e-24,
Organism=Escherichia coli, GI1788510, Length=234, Percent_Identity=30.3418803418803, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI87081838, Length=193, Percent_Identity=33.160621761658, Blast_Score=84, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLUB_YERPE (Q8ZEG0)

Other databases:

- EMBL:   AL590842
- EMBL:   AE009952
- EMBL:   AE017042
- PIR:   AG0269
- RefSeq:   NP_669367.1
- RefSeq:   NP_993349.1
- ProteinModelPortal:   Q8ZEG0
- SMR:   Q8ZEG0
- GeneID:   1147001
- GeneID:   2765771
- GenomeReviews:   AE009952_GR
- GenomeReviews:   AE017042_GR
- GenomeReviews:   AL590842_GR
- KEGG:   ype:YPO2213
- KEGG:   ypk:y2054
- KEGG:   ypm:YP_2010
- HOGENOM:   HBG754478
- OMA:   ELCTRSD
- ProtClustDB:   PRK10700
- BioCyc:   YPES187410:Y2054-MONOMER
- BioCyc:   YPES214092:YPO2213-MONOMER
- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942
- SMART:   SM00363
- TIGRFAMs:   TIGR00093

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4; SSF55120 PsdUridine_synth_cat_dom

EC number: NA

Molecular weight: Translated: 35695; Mature: 35564

Theoretical pI: Translated: 10.86; Mature: 10.86

Prosite motif: PS01149 PSI_RSU; PS50889 S4

Important sites: ACT_SITE 110-110

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.3 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIK
CCHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECCCCEEEECCEEEEEE
ESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFT
CCHHHHEEEHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEHHHHHHH
RELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGSTRQNVG
HHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHCCC
NAAPAATASRRSGPKKRG
CCCCCCHHHHCCCCCCCC
>Mature Secondary Structure 
GEKLQKILARAGHGSRREIEAIIQQGRVSVDGKVSKLGDRVEVTQSTKIRLDGHLLSIK
CHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCHHHHCCCEEEECCCCEEEECCEEEEEE
ESEENVCRVLAYYKPEGELCTRNDPEGRPTVFDRLPKLRGSRWVAVGRLDVNTSGLLLFT
CCHHHHEEEHEEECCCCCCCCCCCCCCCCHHHHHHHHHCCCEEEEEEEEEECCCCEEEEE
TDGELANRLMHPSREVEREYAVRVFGQIDDDKIKQLSRGVQLEDGPAAFRTISYQGGEGI
CCHHHHHHHCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCCEEEEEEECCCCCC
NQWYNVTLTEGRNREVRRLWEAVGVQVSRLIRVRYGDINLPKGLPRGGWTELDLKATNYL
CCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHEECCCCCCCCCCCCCCEEEEHHHHHHH
RELVELDVETVSKLPVEKDRRRVKANQIRRAVKRHTEVSGRQVAGRQGSARKGSTRQNVG
HHHHHHCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCCHHCCC
NAAPAATASRRSGPKKRG
CCCCCCHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11586360; 12142430