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The map label for this gene is metB [H]

Identifier: 153947307

GI number: 153947307

Start: 127184

End: 128344

Strand: Direct

Name: metB [H]

Synonym: YpsIP31758_0121

Alternate gene names: 153947307

Gene position: 127184-128344 (Clockwise)

Preceding gene: 153948951

Following gene: 153947213

Centisome position: 2.69

GC content: 52.2

Gene sequence:

>1161_bases
ATGACGCGTAAACAGGCAACAATAGCAGTCCGTAGCGGGTTGAATGATGACGAGCAATACGGCTGCGTTGTCCCCCCGAT
TCACCTTTCCAGTACTTACAATTTTATTGATTTTAATCAGCCGCGCACGCATGACTATTCACGTCGTGGTAATCCAACGC
GTGATGTTGTCCAACGGGCGCTGGCGGAATTGGAAGGGGGGGCCGGTGCCGTCATGACCAGCAGCGGGATGTCGGCGATT
CATTTGGTTTGCACTACATTCTTACAGCCGGGCGATCTGTTGGTCGCTCCGCATGACTGTTACGGTGGCAGTTACCGTTT
ATTTGACAGCTTGAGCAAGCGTGGTGCGTATCGGGTGTTGTTTGTTGATCAGGGCGATGAAGCGGCACTGAACCGCGCAT
TGGCGGAGAAACCGAAGTTGGTCTTGATTGAAACACCGAGTAATCCATTGCTACGGGTTGTTGATATTGCCGCCATCTGC
CAAGCCGCCCGTGCTGCGGGCGCACTGACGGTTTGTGATAACACCTTCCTCAGCCCCGCCTTACAGCAGCCTCTCTCTCT
CGGGGCCGATTTAGTGGTGCACTCCTGTACCAAATATCTCAATGGTCACTCTGATGTGGTGGCTGGTGCTGTTATTGCGA
AAGATCCAGAACTGGTTGTTGAGCTGGCATGGTGGGCAAATAATATTGGTGTAACCGGTGCTGCGTTTGACAGCTATCTA
CTCCTTCGTGGTTTACGCACGTTATCACCACGCATGGCTCAACAGCAGCGTAACGCGGATGACATTGTGCGTTATTTACA
GCAACAGCCTTTAGTGAAAAAGCTGTATCATCCTTCCCTGCCACAACATCCCGGCCACGAAATAGCCTGCCGCCAGCAAT
CAGGTTTTGGTGCAATGCTCAGTTTTGAGCTGGATGGTGATGAACAGGTCATGCGCCGTTTCCTTTCTGCCCTTGAGCTA
TTTACCTTGGCAGAGTCTTTGGGGGGGGTAGAAAGCCTGATCTCCCATGCAGCGACCATGACCCACGCGGGTATGGCGGC
AGAGGCGCGTATTGCCGCAGGCATTACTGATAGTTTGTTGCGTATTTCCGTGGGTATTGAAGACAGTGAAGATTTGATTG
CTGATTTGGACCACGCGTTCCAATTGGCAGTAACGAGGTAA

Upstream 100 bases:

>100_bases
TATACGCAAAGAAGTTTAGATGTCCAGATGTATTGACGTCCGTAATTTGTGCGTCTACTCTGGCGTAACATTTTCTCGAT
CCCGGCTGGTGGAAATCAAT

Downstream 100 bases:

>100_bases
GCATGAATGCAACAGCGGTAGCAGCGGCGGCAACGGGCCGTCAACTGCATAAATTTGGTGGCAGTAGCCTTGCGGATGTG
AAGTGTTATCTACGGGTGGC

Product: cystathionine gamma-synthase

Products: NA

Alternate protein names: CGS; O-succinylhomoserine (thiol)-lyase [H]

Number of amino acids: Translated: 386; Mature: 385

Protein sequence:

>386_residues
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRALAEKPKLVLIETPSNPLLRVVDIAAIC
QAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALEL
FTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIADLDHAFQLAVTR

Sequences:

>Translated_386_residues
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAI
HLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRALAEKPKLVLIETPSNPLLRVVDIAAIC
QAARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALEL
FTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIADLDHAFQLAVTR
>Mature_385_residues
TRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRALAELEGGAGAVMTSSGMSAIH
LVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVLFVDQGDEAALNRALAEKPKLVLIETPSNPLLRVVDIAAICQ
AARAAGALTVCDNTFLSPALQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYLL
LRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAMLSFELDGDEQVMRRFLSALELF
TLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLLRISVGIEDSEDLIADLDHAFQLAVTR

Specific function: Methionine biosynthesis; second step. [C]

COG id: COG0626

COG function: function code E; Cystathionine beta-lyases/cystathionine gamma-synthases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the trans-sulfuration enzymes family [H]

Homologues:

Organism=Homo sapiens, GI21361334, Length=363, Percent_Identity=41.0468319559229, Blast_Score=265, Evalue=7e-71,
Organism=Homo sapiens, GI299473759, Length=363, Percent_Identity=38.5674931129477, Blast_Score=233, Evalue=2e-61,
Organism=Homo sapiens, GI25453487, Length=362, Percent_Identity=36.7403314917127, Blast_Score=210, Evalue=2e-54,
Organism=Escherichia coli, GI1790375, Length=383, Percent_Identity=83.5509138381201, Blast_Score=668, Evalue=0.0,
Organism=Escherichia coli, GI1789383, Length=342, Percent_Identity=33.0409356725146, Blast_Score=161, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17538055, Length=385, Percent_Identity=47.012987012987, Blast_Score=311, Evalue=4e-85,
Organism=Caenorhabditis elegans, GI17560056, Length=383, Percent_Identity=45.4308093994778, Blast_Score=294, Evalue=5e-80,
Organism=Caenorhabditis elegans, GI71980708, Length=369, Percent_Identity=34.6883468834688, Blast_Score=223, Evalue=2e-58,
Organism=Saccharomyces cerevisiae, GI6319307, Length=390, Percent_Identity=39.7435897435897, Blast_Score=257, Evalue=2e-69,
Organism=Saccharomyces cerevisiae, GI6321254, Length=400, Percent_Identity=34, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6321904, Length=394, Percent_Identity=29.9492385786802, Blast_Score=148, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6323333, Length=381, Percent_Identity=31.496062992126, Blast_Score=145, Evalue=2e-35,
Organism=Saccharomyces cerevisiae, GI6321170, Length=322, Percent_Identity=29.1925465838509, Blast_Score=143, Evalue=5e-35,
Organism=Saccharomyces cerevisiae, GI6322590, Length=283, Percent_Identity=27.208480565371, Blast_Score=76, Evalue=7e-15,
Organism=Saccharomyces cerevisiae, GI6322970, Length=271, Percent_Identity=25.830258302583, Blast_Score=66, Evalue=9e-12,
Organism=Drosophila melanogaster, GI19922554, Length=381, Percent_Identity=42.5196850393701, Blast_Score=306, Evalue=1e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000277
- InterPro:   IPR011821
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF01053 Cys_Met_Meta_PP [H]

EC number: =2.5.1.48 [H]

Molecular weight: Translated: 41791; Mature: 41660

Theoretical pI: Translated: 5.92; Mature: 5.92

Prosite motif: PS00868 CYS_MET_METAB_PP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRA
CCCCHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDEAALNRALAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPA
EEECCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHH
LQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHEEEEEEHHCCCCCCHHHHHHHH
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAML
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEECCCCCCEEE
SFELDGDEQVMRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLL
EEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHE
RISVGIEDSEDLIADLDHAFQLAVTR
EEEECCCCCHHHHHHHHHHHEEEECC
>Mature Secondary Structure 
TRKQATIAVRSGLNDDEQYGCVVPPIHLSSTYNFIDFNQPRTHDYSRRGNPTRDVVQRA
CCCHHHHHHHCCCCCCCCCCCCCCCEECCCCCCEEECCCCCCCCCCCCCCCHHHHHHHH
LAELEGGAGAVMTSSGMSAIHLVCTTFLQPGDLLVAPHDCYGGSYRLFDSLSKRGAYRVL
HHHHCCCCCEEEECCCCHHHHHHHHHHCCCCCEEEECCCCCCCCHHHHHHHHHCCCEEEE
FVDQGDEAALNRALAEKPKLVLIETPSNPLLRVVDIAAICQAARAAGALTVCDNTFLSPA
EEECCCHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHH
LQQPLSLGADLVVHSCTKYLNGHSDVVAGAVIAKDPELVVELAWWANNIGVTGAAFDSYL
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHEECCCHHEEEEEEHHCCCCCCHHHHHHHH
LLRGLRTLSPRMAQQQRNADDIVRYLQQQPLVKKLYHPSLPQHPGHEIACRQQSGFGAML
HHHHHHHCCHHHHHHHCCHHHHHHHHHHCCHHHHHCCCCCCCCCCCCEEEECCCCCCEEE
SFELDGDEQVMRRFLSALELFTLAESLGGVESLISHAATMTHAGMAAEARIAAGITDSLL
EEEECCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCHHHHHHHHCCHHHHE
RISVGIEDSEDLIADLDHAFQLAVTR
EEEECCCCCHHHHHHHHHHHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6361020; 8346018; 9278503; 3307782; 9843488 [H]