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The map label for this gene is queF

Identifier: 153947239

GI number: 153947239

Start: 1206306

End: 1207151

Strand: Reverse

Name: queF

Synonym: YpsIP31758_1004

Alternate gene names: 153947239

Gene position: 1207151-1206306 (Counterclockwise)

Preceding gene: 153948077

Following gene: 153947482

Centisome position: 25.56

GC content: 46.57

Gene sequence:

>846_bases
ATGTCTTCATATCAAAACCACAAGGCGTTGGCGGAACTGACGCTGGGCAAACCAACGGCTTACTGCGACCACTATGATGC
CACGCTGTTGCAGGCTGTGCCGCGCAGCATGAACCGTGAACCGCTTGGCCTATATCCAGACAATTTACCATTCCATGGTG
CTGATATTTGGACGCTATATGAATTATCTTGGTTAAACAGTAATGGCCTGCCGCAAGTTGCTGTGGGCGAAATAAGTTTA
AACGCAGATAGCATCAATCTGATCGAGTCAAAAAGCTTTAAACTTTATCTCAACAGTTTTAACCAAACCATTTTCGCCGA
TAAAGAAAGCGTGCGAATGACCTTACAACGAGATCTGGCTGCCTGTGCTCAAGGTAACGTCAGTGTCGCACTGTATGATC
TGGATGAAATAACGGGCCAACCCATCTCCAATTTCAATGGCGAATGTCTGGATAAACAAGATATTCGTATCGATAGCTAT
GAATTCAATGCTGATTACCTACAAGGTGCTGCGGGTAAAGATCATGTGGAGGAGAGCCTGGTCAGCCATTTGCTAAAATC
TAACTGCCTGATTACCCATCAGCCAGATTGGGGATCAGTACAAATTCACTACCGCGGCCCACAGATTGACCACGAGGCAC
TGCTGCGCTATCTGGTCTCTTTCCGCCACCACAATGAGTTTCATGAACAATGTGTAGAGCGCATTTTTAACGACATTATG
CGTTTTTGTCAGCCTGAAACATTAACCGTTTATGCCCGTTATACCCGTAGAGGCGGGCTGGATATCAATCCATGGCGTTC
CAACACAGATTTTGTACCGCTAACCGGCCGCTTGGCACGGCAATAA

Upstream 100 bases:

>100_bases
GCACCTGAGTGGTAACTGGCCCCAGCTCAGAAATACAGTTAAGTGCGGCATAAATTGATAAACTAGCGCTATTGTACCGA
TCTCTCATCAAGGGAACAGC

Downstream 100 bases:

>100_bases
ATGCGCGCCTGTGCGCAATATTACCGCTCACTGCACGTTAGTATTGGTAAAAAAAGAAAGACAACGTTAAGGTTAATTAC
AGTATTTTACGGCCTTTTCT

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase

Number of amino acids: Translated: 281; Mature: 280

Protein sequence:

>281_residues
MSSYQNHKALAELTLGKPTAYCDHYDATLLQAVPRSMNREPLGLYPDNLPFHGADIWTLYELSWLNSNGLPQVAVGEISL
NADSINLIESKSFKLYLNSFNQTIFADKESVRMTLQRDLAACAQGNVSVALYDLDEITGQPISNFNGECLDKQDIRIDSY
EFNADYLQGAAGKDHVEESLVSHLLKSNCLITHQPDWGSVQIHYRGPQIDHEALLRYLVSFRHHNEFHEQCVERIFNDIM
RFCQPETLTVYARYTRRGGLDINPWRSNTDFVPLTGRLARQ

Sequences:

>Translated_281_residues
MSSYQNHKALAELTLGKPTAYCDHYDATLLQAVPRSMNREPLGLYPDNLPFHGADIWTLYELSWLNSNGLPQVAVGEISL
NADSINLIESKSFKLYLNSFNQTIFADKESVRMTLQRDLAACAQGNVSVALYDLDEITGQPISNFNGECLDKQDIRIDSY
EFNADYLQGAAGKDHVEESLVSHLLKSNCLITHQPDWGSVQIHYRGPQIDHEALLRYLVSFRHHNEFHEQCVERIFNDIM
RFCQPETLTVYARYTRRGGLDINPWRSNTDFVPLTGRLARQ
>Mature_280_residues
SSYQNHKALAELTLGKPTAYCDHYDATLLQAVPRSMNREPLGLYPDNLPFHGADIWTLYELSWLNSNGLPQVAVGEISLN
ADSINLIESKSFKLYLNSFNQTIFADKESVRMTLQRDLAACAQGNVSVALYDLDEITGQPISNFNGECLDKQDIRIDSYE
FNADYLQGAAGKDHVEESLVSHLLKSNCLITHQPDWGSVQIHYRGPQIDHEALLRYLVSFRHHNEFHEQCVERIFNDIMR
FCQPETLTVYARYTRRGGLDINPWRSNTDFVPLTGRLARQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1)

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily

Homologues:

Organism=Escherichia coli, GI1789158, Length=282, Percent_Identity=75.886524822695, Blast_Score=451, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEF_YERP3 (A7FFG0)

Other databases:

- EMBL:   CP000720
- RefSeq:   YP_001399988.1
- ProteinModelPortal:   A7FFG0
- SMR:   A7FFG0
- STRING:   A7FFG0
- GeneID:   5385603
- GenomeReviews:   CP000720_GR
- KEGG:   ypi:YpsIP31758_1004
- eggNOG:   COG0780
- HOGENOM:   HBG289139
- OMA:   PFRSNFE
- ProtClustDB:   PRK11792
- BioCyc:   YPSE349747:YPSIP31758_1004-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00817
- InterPro:   IPR016428
- InterPro:   IPR020602
- PIRSF:   PIRSF004750
- TIGRFAMs:   TIGR03138

Pfam domain/function: PF01227 GTP_cyclohydroI

EC number: =1.7.1.13

Molecular weight: Translated: 32056; Mature: 31925

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSYQNHKALAELTLGKPTAYCDHYDATLLQAVPRSMNREPLGLYPDNLPFHGADIWTLY
CCCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNSNGLPQVAVGEISLNADSINLIESKSFKLYLNSFNQTIFADKESVRMTLQRDLA
EEEECCCCCCCEEEEEEEEECCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHH
ACAQGNVSVALYDLDEITGQPISNFNGECLDKQDIRIDSYEFNADYLQGAAGKDHVEESL
HHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHH
VSHLLKSNCLITHQPDWGSVQIHYRGPQIDHEALLRYLVSFRHHNEFHEQCVERIFNDIM
HHHHHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RFCQPETLTVYARYTRRGGLDINPWRSNTDFVPLTGRLARQ
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHCCC
>Mature Secondary Structure 
SSYQNHKALAELTLGKPTAYCDHYDATLLQAVPRSMNREPLGLYPDNLPFHGADIWTLY
CCCHHHHHHHHEECCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEE
ELSWLNSNGLPQVAVGEISLNADSINLIESKSFKLYLNSFNQTIFADKESVRMTLQRDLA
EEEECCCCCCCEEEEEEEEECCCCEEEEECCCEEEEEECCCCEEEECHHHHHHHHHHHHH
ACAQGNVSVALYDLDEITGQPISNFNGECLDKQDIRIDSYEFNADYLQGAAGKDHVEESL
HHHCCCCEEEEEEHHHHCCCCCCCCCCCCCCCCCCEEEEEECCHHHHCCCCCHHHHHHHH
VSHLLKSNCLITHQPDWGSVQIHYRGPQIDHEALLRYLVSFRHHNEFHEQCVERIFNDIM
HHHHHHCCCEEEECCCCCEEEEEEECCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHH
RFCQPETLTVYARYTRRGGLDINPWRSNTDFVPLTGRLARQ
HHCCCCEEEEEEEECCCCCCCCCCCCCCCCEEECCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA