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The map label for this gene is metE [H]

Identifier: 153947209

GI number: 153947209

Start: 294178

End: 296454

Strand: Direct

Name: metE [H]

Synonym: YpsIP31758_0264

Alternate gene names: 153947209

Gene position: 294178-296454 (Clockwise)

Preceding gene: 153947020

Following gene: 153946882

Centisome position: 6.23

GC content: 49.93

Gene sequence:

>2277_bases
ATGACAATTTTAAATCACACACTGGGTTTTCCGCGTGTTGGTCTGAAACGTGAACTGAAGAAAGCACAAGAAAGTTACTG
GGCAGGCAACTCAACACAGGAAGAATTGCTCAATGTGGGGCGTGAATTGCGTGCTCGCCATTGGCAACAGCAACAACAAG
CGGGCGTTGATTTAGTGCCAGTAGGTGATTTCGCTTGGTATGACCATGTCCTGACAACCAGCCTGTTGCTGGGTAACGTT
CCAGAGCGCCACCAAAATGCTGATGGCTCGATTGATATAGACACGTTGTTCCGTATTGGTCGTGGCCGTGCTCCAACCGG
TAAGCCCGCAGCGGCTGCCGAGATGACTAAATGGTTTAATACCAACTATCACTACATGGTGCCTGAGTTCCAGCAAGGCC
AGCAGTTTAAACTGGGTTGGACTCAACTGCTGGATGAAGTGGACGAAGCGCTGGCCTTGGGCCACAAAATTAAACCTGTG
CTGCTTGGCCCGATCACTTATCTTTGGTTGGGTAAAGTCAAAGGTGAGCAGTTCGATCGCCTCTCGCTACTGAACGATAT
TTTGCCGGTTTACCAGCAGGTGTTGGCTGAATTAGCGAAGCGGGGAATCGAGTGGGTGCAGATTGATGAGCCAGCATTGG
TTCTGGAATTACCACAAGAGTGGTTGGATGCCTATCAACCGGCTTATCAGGCCCTGCAAGGTCAGGTCAAATTATTGCTG
ACAACTTACTTCGACAGTATCGGCCATAACATCGACACCATCCGCGCTCTGCCAGTGCAAGGCTTGCATGTTGATGTGGT
TGCGGGTCATGACGATCTTGCGGTTTTGCATAAAAACTTACCAAAAGAGTGGTTGTTATCTCTGGGGGTTATTAATGGCC
GTAACGTATGGCGTGCTGACCTGAGTAGTTGGTTTGAACGTCTGCAGCCATTGGTTAATAGCCGCCCGCTGTGGTTGGGT
AGCTCATGCTCCTTATTGCACAGCCCGATTGATTTAAATGAAGAGACCCGTCTTGATGCAGAAGTGAAGAGCTGGTTTGC
CTTCGCCCTGCAAAAATGTGCTGAGTTAGCGCTGCTAACACAAGCATTGAATGCACCTAATGACGCTAAGCTGGCAGAAC
TGGCTGCGTACAGTGCGCCGATCCGCGCCCGTCGTTCCTCCAGCCGCGTGCATAATGCGCAAGTTGAGCAGCGTTTAGCT
GCGATTACTTCGCAGGATATTGAGCGCCAACTGCCTTATGAAGCACGTGCTGAAACTCAGCGTAAGCGTTTTAATTTGCC
TGCCTGGCCAACCACGACCATTGGTTCTTTCCCACAAACGACAGAGATCCGTGGTTTACGCCTTGATTTTAAACAAGGTC
GTCTGGACGGTAAAAACTATCGCACAGGGATCAGCGAGCATATCAAACAAGCTATTGCTGAACAGGAACGCTTGGGCCTG
GACGTGCTGGTTCACGGTGAAGCAGAACGTAACGACATGGTCGAATACTTCGGCGAACACTTGGATGGTTTTGTCTTTAC
ACAAAATGGTTGGGTACAGAGCTACGGTTCCCGCTGTGTGAAACCGCCGGTCATCATTGGTGATATTAGCCGCCCAGAAG
CGATTACTGTTGAGTGGGCGAAATATGCTCAATCCTTAACAGAGAAACCGGTAAAAGGCATGCTTACCGGCCCGGTCACT
ATTCTGTGCTGGTCGTTCCCGCGTGAAGATGTCAGCCGTGAGACGATTGCCAAACAAATTGCGCTGGCTTTGCGTGACGA
AGTGGAAGATTTAGAAAAAGCAGGCATTGGTATCATCCAGATTGATGAACCTGCATTACGTGAAGGTTTGCCTCTGCGCC
GTGCTGACTGGCAAGCCTATCTGCAATGGGCCGTCGACGCCTTTAAATTGAATGCCGCTGTGGCGCAAAACGATACACAG
ATCCATACCCACATGTGTTATTGCGAGTTCAATGACATCATGGATTCCATTGCCGCACTGGATGCTGATGTGATTACCAT
CGAAACCTCCCGCTCAGATATGGAACTGTTGGAATCATTTGAAGACTTCGCTTATCCGAATGAAATTGGTCCCGGTGTTT
ATGATATTCATTCACCGAATGTGCCAAGTGTTGAATGGATTGAAGCGTTATTACGCAAAGCCGCTCAACGTATTCCGGCC
GAGCGTTTGTGGGTGAACCCAGATTGTGGTTTGAAAACCCGCGGCTGGCCTGAGACGCGTCAGGCTCTGGCGAATATGGT
GCTGGCAGCGCAGCGTCTGCGTGAAGAACAGATTTAA

Upstream 100 bases:

>100_bases
TCCTTCACAGTGACTGTGAAAAAAATGCGCTTGTGGAATATACAGTACCTGCGGATTATAGATGTGTAAACATCTGGATG
GCTAAATGGGGAATAAGACG

Downstream 100 bases:

>100_bases
TTAAACCGTTTTACTCTGTGTAGTGGGGCTTTTGGGGCGCTTTCGCGCCCTTTTTTATTGCGTTTCTTCTGGAATGGGGA
TGGCCGAGACACCATGTTGG

Product: 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase

Products: NA

Alternate protein names: Cobalamin-independent methionine synthase; Methionine synthase, vitamin-B12 independent isozyme [H]

Number of amino acids: Translated: 758; Mature: 757

Protein sequence:

>758_residues
MTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNV
PERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPV
LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLL
TTYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSRPLWLG
SSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLA
AITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKQAIAEQERLGL
DVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGMLTGPVT
ILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQ
IHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPA
ERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQI

Sequences:

>Translated_758_residues
MTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNV
PERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPV
LLGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLL
TTYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSRPLWLG
SSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLA
AITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKQAIAEQERLGL
DVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGMLTGPVT
ILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQ
IHTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPA
ERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQI
>Mature_757_residues
TILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVPVGDFAWYDHVLTTSLLLGNVP
ERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFNTNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVL
LGPITYLWLGKVKGEQFDRLSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLLT
TYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRADLSSWFERLQPLVNSRPLWLGS
SCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLTQALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLAA
ITSQDIERQLPYEARAETQRKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKQAIAEQERLGLD
VLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWAKYAQSLTEKPVKGMLTGPVTI
LCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQIDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQI
HTHMCYCEFNDIMDSIAALDADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPAE
RLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQI

Specific function: Catalyzes the transfer of a methyl group from 5- methyltetrahydrofolate to homocysteine resulting in methionine formation [H]

COG id: COG0620

COG function: function code E; Methionine synthase II (cobalamin-independent)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the vitamin-B12 independent methionine synthase family [H]

Homologues:

Organism=Escherichia coli, GI2367304, Length=754, Percent_Identity=85.2785145888594, Blast_Score=1335, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6320936, Length=769, Percent_Identity=47.9843953185956, Blast_Score=709, Evalue=0.0,

Paralogues:

None

Copy number: 45,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013215
- InterPro:   IPR006276
- InterPro:   IPR002629 [H]

Pfam domain/function: PF08267 Meth_synt_1; PF01717 Meth_synt_2 [H]

EC number: =2.1.1.14 [H]

Molecular weight: Translated: 85676; Mature: 85545

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVP
CCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVLLGPITYLWLGKVKGEQFDR
CCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
TTYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRAD
HHHHHHHCCCCHHEEECCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCCHHHHH
LSSWFERLQPLVNSRPLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLT
HHHHHHHHHHHHCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLAAITSQDIERQLPYEARAETQ
HHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKQAIAEQERLGL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCC
DVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWA
EEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHH
KYAQSLTEKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQ
HHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQIHTHMCYCEFNDIMDSIAAL
ECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHC
DADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPA
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCH
ERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQI
HHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TILNHTLGFPRVGLKRELKKAQESYWAGNSTQEELLNVGRELRARHWQQQQQAGVDLVP
CCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEE
VGDFAWYDHVLTTSLLLGNVPERHQNADGSIDIDTLFRIGRGRAPTGKPAAAAEMTKWFN
CCCHHHHHHHHHHHHHHCCCCHHHCCCCCCEEHHHHHHHCCCCCCCCCCHHHHHHHHHHC
TNYHYMVPEFQQGQQFKLGWTQLLDEVDEALALGHKIKPVLLGPITYLWLGKVKGEQFDR
CCCEEECCCCCCCCEEECCHHHHHHHHHHHHHHCCCCCCCEECHHHHHHHHCCCCCHHHH
LSLLNDILPVYQQVLAELAKRGIEWVQIDEPALVLELPQEWLDAYQPAYQALQGQVKLLL
HHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEECCHHHHHHHHHHHHHHHHHHHHHH
TTYFDSIGHNIDTIRALPVQGLHVDVVAGHDDLAVLHKNLPKEWLLSLGVINGRNVWRAD
HHHHHHHCCCCHHEEECCCCCEEEEEEECCCHHHHHHHCCCHHHHHHHCCCCCCCHHHHH
LSSWFERLQPLVNSRPLWLGSSCSLLHSPIDLNEETRLDAEVKSWFAFALQKCAELALLT
HHHHHHHHHHHHCCCCCEECCCHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
QALNAPNDAKLAELAAYSAPIRARRSSSRVHNAQVEQRLAAITSQDIERQLPYEARAETQ
HHHCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
RKRFNLPAWPTTTIGSFPQTTEIRGLRLDFKQGRLDGKNYRTGISEHIKQAIAEQERLGL
HHHCCCCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCCHHHCCHHHHHHHHHHHHHHCCC
DVLVHGEAERNDMVEYFGEHLDGFVFTQNGWVQSYGSRCVKPPVIIGDISRPEAITVEWA
EEEEECCCCHHHHHHHHHHHCCCEEEECCCHHHHHCCCCCCCCEEEECCCCCCEEEEHHH
KYAQSLTEKPVKGMLTGPVTILCWSFPREDVSRETIAKQIALALRDEVEDLEKAGIGIIQ
HHHHHHHHCCCCCEECCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEE
IDEPALREGLPLRRADWQAYLQWAVDAFKLNAAVAQNDTQIHTHMCYCEFNDIMDSIAAL
ECCHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEEEHHHHHHHHHHC
DADVITIETSRSDMELLESFEDFAYPNEIGPGVYDIHSPNVPSVEWIEALLRKAAQRIPA
CCCEEEEECCCHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHCCH
ERLWVNPDCGLKTRGWPETRQALANMVLAAQRLREEQI
HHEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA