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The map label for this gene is outO [H]

Identifier: 153947137

GI number: 153947137

Start: 1151034

End: 1151867

Strand: Direct

Name: outO [H]

Synonym: YpsIP31758_0963

Alternate gene names: 153947137

Gene position: 1151034-1151867 (Clockwise)

Preceding gene: 153947794

Following gene: 153946971

Centisome position: 24.37

GC content: 35.85

Gene sequence:

>834_bases
ATGCAGTTTTTTTTTGTGGGCTATTTAATTTTAGGGGCCATGGTAGGCAGCTTTCTTAATGTTTTAATCTATAGATTGCC
TATTATGCTGGCTAATTTATCATCACGATCTGAGTCACACGGTGAAGAAATTAAGATGCGTTCTCATTTGAGAAATATTA
ATTTATTTCAACCCGGCTCTTTTTGCCATCATTGCAATGAGTCAATACCAATAAAATATAATATACCGATACTCGGTTGG
ATTTTTCTGCGTGGTGCCAGTCGTTGTTGTAATAAGAAGATAAGCACCCGCTATCTATTTATTGAAGTATTAGCTGTCAT
TCAAACACTCTTGGTTCTTATGATTTTTAAAGAGGACTTACTTATCTGCACTAGCTTGGTGCTAATTTGGTCACTTACGG
CATTGGCATTTATAGATTTCGATACCTACCTCCTTCCTGATTGTATGACAATACCTTTGCTGTGGCTTGGTCTTCTTATC
AATATAGATACGGTTTTTGCACCACTAACCTCTGCAGTACTGGGTGCTGTCAGTGGATACCTTTTTCTTTGGCTATCATA
TTGGTTGTTTAAAATAGTTAGAGGTGTTGATGGAATGGGCTATGGTGATTTTAAATTAATGGCCGCATTAGGTGCTTGGT
TTGGTGTGAGTGCAGTACCTTTTTTAATATTGTTCTCTTCCTTTTTTGGTCTTGTCGCATATGCTATTTTTTATTTTTTT
GATAAAAAAGATAATGGAAAAGAGATTAATTACATCGCATTTGGCCCTTATATTTCATTGGCGGGTGTTCTATACCTGTT
TCTTGGGAGTCATGTGACTAATTTATTTTCTTAA

Upstream 100 bases:

>100_bases
GATATATATGTTCACCAAGATTATGATGATGGGTATGTTGCAGGACAAATTATATTCTTTAAATGAACCCCACGGTCTGA
TAGAGGTAAATATGATGGAT

Downstream 100 bases:

>100_bases
TAAATGGGGTTAATAGCTCCTCTAACTCCAGACACTTAGTGTTTTTAGCAGCCAAATCTGATTTGATATCTTGATATAGA
GATTCACTTTTCTTTATGAT

Product: type IV prepilin peptidase

Products: NA

Alternate protein names: Pectic enzymes secretion protein outO; Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 277; Mature: 277

Protein sequence:

>277_residues
MQFFFVGYLILGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPILGW
IFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLGLLI
NIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIFYFF
DKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Sequences:

>Translated_277_residues
MQFFFVGYLILGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPILGW
IFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLGLLI
NIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIFYFF
DKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS
>Mature_277_residues
MQFFFVGYLILGAMVGSFLNVLIYRLPIMLANLSSRSESHGEEIKMRSHLRNINLFQPGSFCHHCNESIPIKYNIPILGW
IFLRGASRCCNKKISTRYLFIEVLAVIQTLLVLMIFKEDLLICTSLVLIWSLTALAFIDFDTYLLPDCMTIPLLWLGLLI
NIDTVFAPLTSAVLGAVSGYLFLWLSYWLFKIVRGVDGMGYGDFKLMAALGAWFGVSAVPFLILFSSFFGLVAYAIFYFF
DKKDNGKEINYIAFGPYISLAGVLYLFLGSHVTNLFS

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue. Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of the multiple pectic enzymes [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=259, Percent_Identity=37.0656370656371, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1789732, Length=133, Percent_Identity=45.1127819548872, Blast_Score=81, Evalue=6e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 31287; Mature: 31287

Theoretical pI: Translated: 8.11; Mature: 8.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure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CCCCCCCEEEEEEECHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8326859; 8065262 [H]