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The map label for this gene is yuxG [H]

Identifier: 153947055

GI number: 153947055

Start: 2614074

End: 2616140

Strand: Reverse

Name: yuxG [H]

Synonym: YpsIP31758_2311

Alternate gene names: 153947055

Gene position: 2616140-2614074 (Counterclockwise)

Preceding gene: 153949096

Following gene: 153948466

Centisome position: 55.39

GC content: 52.3

Gene sequence:

>2067_bases
ATGAAAAGTCTTTGGAGTGATGCCGATGCGGCTGAGTGTGTTGAAAGGCTAGGTTTACTGGGGCTACCGCCCGAGTTGGG
GCTTCGCACTTACTCTGCACGCTTGTTAGGGTCGAACCCCAAGCTTGTCTTACACGGAGGGGGAAATACCTCGGTTAAGC
TCAATATTAATGATCTTTTCGGTAACCCCGTACAGGTGATGTGTATTAAAGGTAGCGGCCAAGACTTAGCCACAATCACC
GCGTCAGGTCACCCGGCGGTCAGATTGGCACCGCTGCAACAACTGAAGGTATTGGATCATCTGTCTGATGAAGACATGGT
CAATATCTTACGCACGAATCTTCTCGACTGCCGTGCGCCGACCCCCTCGGTTGAAGCACTTTTACATGCGTTTGTTGACC
GGCCTGTTATTGATCATACCCATGCGGTTGCTGCCATGGTCATTGCTGATCAACCTGATGGTGACCGTTTGTGCCGCTAC
ATCTATGGTGAGCGTGTGCTGTGGATCCCCTATGTCATGTCTGGATTTGCGCTCTCACTCGCGGTCGAACGTGCCTTCCG
GGCGTACCCGGATGCGGAGGGTATGCTCTTGGGTAAGCATGGCCTGTGTACGTTTGGTGAGAGTGCCAAAGCCTCTTATG
AGCGAATGATTACGTTTGTCACCCTGGCTGAAAATTTTATTGAAAGCCGCCGGGCCATATATTCCCCGCCAGTGATAACG
CCCCCCGCAAAGCCCAACAATTTGGCTGACATGGCACACATTGCGCCGTATCTACGTGCTGCTTTTGCTCGTTCAAGTGC
GATAAGTGAAAAAAAACATTGGATATTCGATTTACGTACTGACCCAAAGAGTCTCGCGTTTTCATCTGGCTTAGGAACCT
CAGACATTGCCCAGCGTGGCGTGGCGACCCCAGATCATGTTATCCGCATGAAAGGCCGCCCGGTGGTTTTAGATGCGCCT
GACCTGAATAATTTGGGCGACTGGGCCGCACAGACACAGACAAAAATCGCGGCGTTTGTTGAGCGCTATCAGCACTATTT
TCATCGTAATAATGCCCGGTTTGAGAGGGGGAAAATAGCACTGGACGCCCTACCAAGAGCCATTGTCATTCCGTATCTCG
GGGTGGTGGGGGCGGGCAGAACTGCAGAGGACGCGGCAATCAACGCCGATATCATCGAGTCATGGCTGGATGTCGTGACG
GATGCCACTGTCTGTGGTGATTATGAGCCGATTGGTGAAGCGGACGAATTCGACATGGAATATTGGTCTTTGGAGCAAGC
GAAGATCGATAAAGCGTCGGTAAAACCATTACAAGGGCAGATCGTGGTGGTCACGGGGGCGGGGGGCGGTATTGGCGCGG
CTATCGCCAAGGAGTTTTCGTTATTAGGGGCAGAACTCGCGGTACTCGATATTGATAGCGAATCAGCCAAAAACGTTGCC
GCGCAATTAGGACCACATGCACTGGCTTTACAGTGCGATGTCACCGAGACCGCTTCTGTTCAGGCGGCTTTTGAGACGAT
AGCGACCCGATTTGGCGGCGTTGATATCGTGGTATCCAATGCGGGCGTTGCGTTATCCGGGGCGATAGCTGAACTGCCGG
AAGCCACACTGCGTACCAGCTTTGAAGTGAACTTTTTTGCCCATCAACGGGTGGCCCAACAGGCGGTCAGCATCATGAAA
AAACAGGGTATCGGTGGGGTGCTGCTGTTTAACATTTCTAAACAAGCGATCAACCCTGGTATTAACTTTGGCGCTTATGG
CACCTCAAAGGCCGCCTTGTTAAGTCTGGTGCGGCAATATGCGTTGGAGCAGGGTCAGGATAGCATCCGAGTTAATGCAG
TTAATGCGGATCGTATTCGGTCTGGTTTACTTGATGATGAAATGATTAGCCTGAGGGCCAGGGCGCGCGGCCTGAGCGAA
GAGAAGTACATGGCGGGTAATTTACTTGGTCAAGAGGTCACCGCTCAAGATGTCGCCAAGGCTTTTGTCGTCTCGGCCAT
GCTCGATAAATCAACCGGTAACGTCATCACCGTGGATGGTGGCAATGTCGCAGCGATGTTGCGATAA

Upstream 100 bases:

>100_bases
TTCTTCCTCTTGACGGCCATTACATTTGTAAGGCATACAAATCATATGTAATGACATAAGTAACTAGCATAGAACATATG
TTACTACTTACGAGGTGAAG

Downstream 100 bases:

>100_bases
ATCATGGTTTTCCTTCGTTCAGGTTTTTACCGATATAAATCCTGAGGGGAGCACTGATTAGGAGCCTCAAACCGTTATCG
TGATTCAAACCGTTACCGTA

Product: short chain dehydrogenase

Products: 3alpha12alpha-dihydroxy-oxobeta-cholanate; NADH [C]

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 688; Mature: 688

Protein sequence:

>688_residues
MKSLWSDADAAECVERLGLLGLPPELGLRTYSARLLGSNPKLVLHGGGNTSVKLNINDLFGNPVQVMCIKGSGQDLATIT
ASGHPAVRLAPLQQLKVLDHLSDEDMVNILRTNLLDCRAPTPSVEALLHAFVDRPVIDHTHAVAAMVIADQPDGDRLCRY
IYGERVLWIPYVMSGFALSLAVERAFRAYPDAEGMLLGKHGLCTFGESAKASYERMITFVTLAENFIESRRAIYSPPVIT
PPAKPNNLADMAHIAPYLRAAFARSSAISEKKHWIFDLRTDPKSLAFSSGLGTSDIAQRGVATPDHVIRMKGRPVVLDAP
DLNNLGDWAAQTQTKIAAFVERYQHYFHRNNARFERGKIALDALPRAIVIPYLGVVGAGRTAEDAAINADIIESWLDVVT
DATVCGDYEPIGEADEFDMEYWSLEQAKIDKASVKPLQGQIVVVTGAGGGIGAAIAKEFSLLGAELAVLDIDSESAKNVA
AQLGPHALALQCDVTETASVQAAFETIATRFGGVDIVVSNAGVALSGAIAELPEATLRTSFEVNFFAHQRVAQQAVSIMK
KQGIGGVLLFNISKQAINPGINFGAYGTSKAALLSLVRQYALEQGQDSIRVNAVNADRIRSGLLDDEMISLRARARGLSE
EKYMAGNLLGQEVTAQDVAKAFVVSAMLDKSTGNVITVDGGNVAAMLR

Sequences:

>Translated_688_residues
MKSLWSDADAAECVERLGLLGLPPELGLRTYSARLLGSNPKLVLHGGGNTSVKLNINDLFGNPVQVMCIKGSGQDLATIT
ASGHPAVRLAPLQQLKVLDHLSDEDMVNILRTNLLDCRAPTPSVEALLHAFVDRPVIDHTHAVAAMVIADQPDGDRLCRY
IYGERVLWIPYVMSGFALSLAVERAFRAYPDAEGMLLGKHGLCTFGESAKASYERMITFVTLAENFIESRRAIYSPPVIT
PPAKPNNLADMAHIAPYLRAAFARSSAISEKKHWIFDLRTDPKSLAFSSGLGTSDIAQRGVATPDHVIRMKGRPVVLDAP
DLNNLGDWAAQTQTKIAAFVERYQHYFHRNNARFERGKIALDALPRAIVIPYLGVVGAGRTAEDAAINADIIESWLDVVT
DATVCGDYEPIGEADEFDMEYWSLEQAKIDKASVKPLQGQIVVVTGAGGGIGAAIAKEFSLLGAELAVLDIDSESAKNVA
AQLGPHALALQCDVTETASVQAAFETIATRFGGVDIVVSNAGVALSGAIAELPEATLRTSFEVNFFAHQRVAQQAVSIMK
KQGIGGVLLFNISKQAINPGINFGAYGTSKAALLSLVRQYALEQGQDSIRVNAVNADRIRSGLLDDEMISLRARARGLSE
EKYMAGNLLGQEVTAQDVAKAFVVSAMLDKSTGNVITVDGGNVAAMLR
>Mature_688_residues
MKSLWSDADAAECVERLGLLGLPPELGLRTYSARLLGSNPKLVLHGGGNTSVKLNINDLFGNPVQVMCIKGSGQDLATIT
ASGHPAVRLAPLQQLKVLDHLSDEDMVNILRTNLLDCRAPTPSVEALLHAFVDRPVIDHTHAVAAMVIADQPDGDRLCRY
IYGERVLWIPYVMSGFALSLAVERAFRAYPDAEGMLLGKHGLCTFGESAKASYERMITFVTLAENFIESRRAIYSPPVIT
PPAKPNNLADMAHIAPYLRAAFARSSAISEKKHWIFDLRTDPKSLAFSSGLGTSDIAQRGVATPDHVIRMKGRPVVLDAP
DLNNLGDWAAQTQTKIAAFVERYQHYFHRNNARFERGKIALDALPRAIVIPYLGVVGAGRTAEDAAINADIIESWLDVVT
DATVCGDYEPIGEADEFDMEYWSLEQAKIDKASVKPLQGQIVVVTGAGGGIGAAIAKEFSLLGAELAVLDIDSESAKNVA
AQLGPHALALQCDVTETASVQAAFETIATRFGGVDIVVSNAGVALSGAIAELPEATLRTSFEVNFFAHQRVAQQAVSIMK
KQGIGGVLLFNISKQAINPGINFGAYGTSKAALLSLVRQYALEQGQDSIRVNAVNADRIRSGLLDDEMISLRARARGLSE
EKYMAGNLLGQEVTAQDVAKAFVVSAMLDKSTGNVITVDGGNVAAMLR

Specific function: 7-Alpha-Dehydroxylation Of Cholic Acid, Yielding Deoxycholic Acid And Lithocholic Acid, Respectively. Highest Affinity With Taurochenodeoxycholic Acid. [C]

COG id: COG3347

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI32483357, Length=259, Percent_Identity=27.7992277992278, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI40254992, Length=243, Percent_Identity=28.3950617283951, Blast_Score=75, Evalue=3e-13,
Organism=Homo sapiens, GI40807363, Length=215, Percent_Identity=28.8372093023256, Blast_Score=74, Evalue=5e-13,
Organism=Homo sapiens, GI126723191, Length=189, Percent_Identity=29.1005291005291, Blast_Score=70, Evalue=7e-12,
Organism=Homo sapiens, GI7705925, Length=259, Percent_Identity=30.1158301158301, Blast_Score=69, Evalue=1e-11,
Organism=Homo sapiens, GI31542939, Length=198, Percent_Identity=27.2727272727273, Blast_Score=67, Evalue=4e-11,
Organism=Escherichia coli, GI1787905, Length=252, Percent_Identity=34.5238095238095, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI87082100, Length=190, Percent_Identity=31.5789473684211, Blast_Score=79, Evalue=1e-15,
Organism=Escherichia coli, GI1786812, Length=254, Percent_Identity=29.9212598425197, Blast_Score=72, Evalue=1e-13,
Organism=Escherichia coli, GI1787335, Length=252, Percent_Identity=27.3809523809524, Blast_Score=71, Evalue=2e-13,
Organism=Escherichia coli, GI1790717, Length=262, Percent_Identity=26.3358778625954, Blast_Score=70, Evalue=5e-13,
Organism=Escherichia coli, GI87082160, Length=256, Percent_Identity=25.78125, Blast_Score=69, Evalue=9e-13,
Organism=Escherichia coli, GI1789208, Length=266, Percent_Identity=27.0676691729323, Blast_Score=69, Evalue=1e-12,
Organism=Escherichia coli, GI1789378, Length=194, Percent_Identity=29.3814432989691, Blast_Score=65, Evalue=2e-11,
Organism=Escherichia coli, GI1789057, Length=255, Percent_Identity=25.8823529411765, Blast_Score=64, Evalue=3e-11,
Organism=Caenorhabditis elegans, GI17567345, Length=164, Percent_Identity=29.2682926829268, Blast_Score=74, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17555706, Length=259, Percent_Identity=27.4131274131274, Blast_Score=68, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI193203115, Length=113, Percent_Identity=31.858407079646, Blast_Score=66, Evalue=6e-11,
Organism=Caenorhabditis elegans, GI193203117, Length=119, Percent_Identity=30.2521008403361, Blast_Score=65, Evalue=8e-11,
Organism=Caenorhabditis elegans, GI17538182, Length=210, Percent_Identity=25.2380952380952, Blast_Score=65, Evalue=9e-11,
Organism=Drosophila melanogaster, GI24643142, Length=182, Percent_Identity=34.6153846153846, Blast_Score=79, Evalue=1e-14,
Organism=Drosophila melanogaster, GI28571526, Length=264, Percent_Identity=28.7878787878788, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24641232, Length=205, Percent_Identity=28.780487804878, Blast_Score=76, Evalue=6e-14,
Organism=Drosophila melanogaster, GI28571387, Length=203, Percent_Identity=26.6009852216749, Blast_Score=75, Evalue=1e-13,
Organism=Drosophila melanogaster, GI21355319, Length=271, Percent_Identity=29.1512915129151, Blast_Score=74, Evalue=4e-13,
Organism=Drosophila melanogaster, GI21357041, Length=266, Percent_Identity=27.0676691729323, Blast_Score=72, Evalue=1e-12,
Organism=Drosophila melanogaster, GI23397609, Length=259, Percent_Identity=29.7297297297297, Blast_Score=72, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24644339, Length=271, Percent_Identity=27.6752767527675, Blast_Score=70, Evalue=5e-12,
Organism=Drosophila melanogaster, GI24640492, Length=222, Percent_Identity=22.5225225225225, Blast_Score=68, Evalue=2e-11,
Organism=Drosophila melanogaster, GI116007236, Length=202, Percent_Identity=26.7326732673267, Blast_Score=67, Evalue=4e-11,
Organism=Drosophila melanogaster, GI281364494, Length=209, Percent_Identity=26.7942583732057, Blast_Score=66, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001303
- InterPro:   IPR013454
- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040 [H]

Pfam domain/function: PF00106 adh_short; PF00596 Aldolase_II [H]

EC number: 1.1.1.159 [C]

Molecular weight: Translated: 73842; Mature: 73842

Theoretical pI: Translated: 5.78; Mature: 5.78

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSLWSDADAAECVERLGLLGLPPELGLRTYSARLLGSNPKLVLHGGGNTSVKLNINDLF
CCCCCCCCCHHHHHHHCCCCCCCHHHCCCHHHHHEECCCCEEEEECCCCCEEEEEEHHHC
GNPVQVMCIKGSGQDLATITASGHPAVRLAPLQQLKVLDHLSDEDMVNILRTNLLDCRAP
CCCEEEEEEECCCCCEEEEEECCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TPSVEALLHAFVDRPVIDHTHAVAAMVIADQPDGDRLCRYIYGERVLWIPYVMSGFALSL
CCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHCCEEEEHHHHHHHHHHHH
AVERAFRAYPDAEGMLLGKHGLCTFGESAKASYERMITFVTLAENFIESRRAIYSPPVIT
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PPAKPNNLADMAHIAPYLRAAFARSSAISEKKHWIFDLRTDPKSLAFSSGLGTSDIAQRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCHHHHHHCC
VATPDHVIRMKGRPVVLDAPDLNNLGDWAAQTQTKIAAFVERYQHYFHRNNARFERGKIA
CCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LDALPRAIVIPYLGVVGAGRTAEDAAINADIIESWLDVVTDATVCGDYEPIGEADEFDME
HHHCCHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCH
YWSLEQAKIDKASVKPLQGQIVVVTGAGGGIGAAIAKEFSLLGAELAVLDIDSESAKNVA
HHHHHHHHCCHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHH
AQLGPHALALQCDVTETASVQAAFETIATRFGGVDIVVSNAGVALSGAIAELPEATLRTS
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEECHHHHCCHHHHHCE
FEVNFFAHQRVAQQAVSIMKKQGIGGVLLFNISKQAINPGINFGAYGTSKAALLSLVRQY
EEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALEQGQDSIRVNAVNADRIRSGLLDDEMISLRARARGLSEEKYMAGNLLGQEVTAQDVAK
HHHCCCCCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
AFVVSAMLDKSTGNVITVDGGNVAAMLR
HHHHHHHHHCCCCCEEEECCCCEEEEEC
>Mature Secondary Structure
MKSLWSDADAAECVERLGLLGLPPELGLRTYSARLLGSNPKLVLHGGGNTSVKLNINDLF
CCCCCCCCCHHHHHHHCCCCCCCHHHCCCHHHHHEECCCCEEEEECCCCCEEEEEEHHHC
GNPVQVMCIKGSGQDLATITASGHPAVRLAPLQQLKVLDHLSDEDMVNILRTNLLDCRAP
CCCEEEEEEECCCCCEEEEEECCCCCEEECHHHHHHHHHCCCCHHHHHHHHHHHHHCCCC
TPSVEALLHAFVDRPVIDHTHAVAAMVIADQPDGDRLCRYIYGERVLWIPYVMSGFALSL
CCHHHHHHHHHHCCCCCCCHHHEEEEEEECCCCHHHHHHHHHCCEEEEHHHHHHHHHHHH
AVERAFRAYPDAEGMLLGKHGLCTFGESAKASYERMITFVTLAENFIESRRAIYSPPVIT
HHHHHHHHCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
PPAKPNNLADMAHIAPYLRAAFARSSAISEKKHWIFDLRTDPKSLAFSSGLGTSDIAQRG
CCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHCCCCCCHHHHHHCC
VATPDHVIRMKGRPVVLDAPDLNNLGDWAAQTQTKIAAFVERYQHYFHRNNARFERGKIA
CCCCCCEEEECCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEE
LDALPRAIVIPYLGVVGAGRTAEDAAINADIIESWLDVVTDATVCGDYEPIGEADEFDME
HHHCCHHHHHHHHHHCCCCCCCCHHHCCHHHHHHHHHHHHCCHHCCCCCCCCCCCCCCCH
YWSLEQAKIDKASVKPLQGQIVVVTGAGGGIGAAIAKEFSLLGAELAVLDIDSESAKNVA
HHHHHHHHCCHHCCCCCCCEEEEEECCCCCHHHHHHHHHHHHCCEEEEEECCCHHHHHHH
AQLGPHALALQCDVTETASVQAAFETIATRFGGVDIVVSNAGVALSGAIAELPEATLRTS
HHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCEEEEEECCCEEEECHHHHCCHHHHHCE
FEVNFFAHQRVAQQAVSIMKKQGIGGVLLFNISKQAINPGINFGAYGTSKAALLSLVRQY
EEEEHHHHHHHHHHHHHHHHHCCCCEEEEEECCHHHCCCCCCCCCCCCHHHHHHHHHHHH
ALEQGQDSIRVNAVNADRIRSGLLDDEMISLRARARGLSEEKYMAGNLLGQEVTAQDVAK
HHHCCCCCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCHHHHHHHHHCCCHHHHHHHHH
AFVVSAMLDKSTGNVITVDGGNVAAMLR
HHHHHHHHHCCCCCEEEECCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 2-Mercaptoethanol; Dithiothreitol; NAD+; NADH [C]

Metal ions: CaCl2; KCl; MnCl2; NaCl [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.8 {cholic} 0.24 {taurochenodeoxycholic} 0.085 {glycochenodeoxycholic} 1 {glycocholic} 0.06 {chenodeoxycholic} [C]

Substrates: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate!; NAD [C]

Specific reaction: @ALPH01.txt*3alpha7alpha12alpha-Trihydroxybeta-cholanate! + NAD+ = 3alpha12alpha-dihydroxy-oxobeta-cholanate + NADH [C]

General reaction: Redox reaction [C]

Inhibitor: Ascorbicacid; BaCl2; CoCl2; CuCl2; EDTA; FeCl3; HgCl2; MgCl2; NH4Cl; Potassiumoxalate; Sodiumcitrate; Sodiumlaurylsulfate; Sodiumperchlorate; Sodiumperiodate; Sodiumpersulfate; TritonX-100; Tween; ZnCl2 [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9274030; 9384377; 8188684 [H]