Click here to switch to the map view.

The map label for this gene is traC [H]

Identifier: 153947023

GI number: 153947023

Start: 4161587

End: 4162558

Strand: Reverse

Name: traC [H]

Synonym: YpsIP31758_3690

Alternate gene names: 153947023

Gene position: 4162558-4161587 (Counterclockwise)

Preceding gene: 153950034

Following gene: 153949752

Centisome position: 88.13

GC content: 49.38

Gene sequence:

>972_bases
ATGAAAAAATCGACTTCTTGCCGTAAGGCTCACAAACGCGCTGATTTATATCAACAGGTCACAGATAAAATTATTGTGGC
ACTGGAAAAAGGCACTATTCCCTGGCGCAAGCCCTGGCGTTCAGTAAGAAAGCCAGAGGGCAGTTCGCTGCCAACCAATG
CATTGACAGGGCGTCCGTATCAAGGGGTGAATATCCCGTTGTTATGGATGGCGGGTGAAGAGCGAGGTTTTGGCTCTGAT
CGCTGGCTGACCTATCGCCAAGCTCAGAAAGCGGGTGGTCAGGTGCGAAAAGGCGAGAGCTGTAGCCTTGCTGTTATTTT
CAAACCGTTTGAAAAACAAGCTGAAGATGAGCAGGGTAATAAGCTGTTTGATGGCGCAGGAAAACCGCTCATGAAACGCT
GCTCGATGATGAAGTCCTTGCAGTTGTTTAACACGGATCAGTGTGAAGGTCTGCCGGAGCTTATGGCGACTCAGCCTGTT
CAACTGATGGTTCAGGATGAAATTGAGATCCTAAGTGCGCCGTTGATGAATCGGGTAGTGGAAATACTCAATCACAGTGG
TGTGAAAACCACACCGAGAAGACAGAATCGGGCATTTTATCGACCATTAACGGACGAAATTATCATGCCGCTCGTTGGGC
AGTTTTTTACCGAAGCAGATTATTGGTCCACGCTGTTGCACGAACTGATCCACGCGAGTGGCCATCAGAAGCGCCTGAAC
CGCGAGGGAATAACCTCGTCAACTCGCACTTTTGGCGATCCCATTTATGCGTTTGAGGAGTTGATTGCAGAACTGGGCAG
CGCTTTTCTCTGCGCGGAGCTGGGTATTTATGGCGAGGTGCAACACGAGAGTTATATCAGTGGTTGGCTGAAAAGGCTCA
GAAAAGACAAAAAGGCGCTATTCCGCGCCTGCCGACAAGCACGTCAGGCGTCTGAGTTTTTGTTAACCCAACAAATTGGT
AACGACGGCTAA

Upstream 100 bases:

>100_bases
GTCATTTAATGACATATCCATTTGGGATCTCGATCCCCTTTTATCCACCTGTGGCGCACTCCGCCACTGGCTGGGGTGCG
CCTTTTTTGAGGTTCATCCC

Downstream 100 bases:

>100_bases
GACAATGCCATAACGGCAGTTTCGATTTGAGTCAGTAACCAAACCCATCGGGGGCCTTGTCCCTGTGGGGCGGCTCCCTC
TTTTCTATGATCAAAAGAGG

Product: antirestriction protein

Products: NA

Alternate protein names: Replication primase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKKSTSCRKAHKRADLYQQVTDKIIVALEKGTIPWRKPWRSVRKPEGSSLPTNALTGRPYQGVNIPLLWMAGEERGFGSD
RWLTYRQAQKAGGQVRKGESCSLAVIFKPFEKQAEDEQGNKLFDGAGKPLMKRCSMMKSLQLFNTDQCEGLPELMATQPV
QLMVQDEIEILSAPLMNRVVEILNHSGVKTTPRRQNRAFYRPLTDEIIMPLVGQFFTEADYWSTLLHELIHASGHQKRLN
REGITSSTRTFGDPIYAFEELIAELGSAFLCAELGIYGEVQHESYISGWLKRLRKDKKALFRACRQARQASEFLLTQQIG
NDG

Sequences:

>Translated_323_residues
MKKSTSCRKAHKRADLYQQVTDKIIVALEKGTIPWRKPWRSVRKPEGSSLPTNALTGRPYQGVNIPLLWMAGEERGFGSD
RWLTYRQAQKAGGQVRKGESCSLAVIFKPFEKQAEDEQGNKLFDGAGKPLMKRCSMMKSLQLFNTDQCEGLPELMATQPV
QLMVQDEIEILSAPLMNRVVEILNHSGVKTTPRRQNRAFYRPLTDEIIMPLVGQFFTEADYWSTLLHELIHASGHQKRLN
REGITSSTRTFGDPIYAFEELIAELGSAFLCAELGIYGEVQHESYISGWLKRLRKDKKALFRACRQARQASEFLLTQQIG
NDG
>Mature_323_residues
MKKSTSCRKAHKRADLYQQVTDKIIVALEKGTIPWRKPWRSVRKPEGSSLPTNALTGRPYQGVNIPLLWMAGEERGFGSD
RWLTYRQAQKAGGQVRKGESCSLAVIFKPFEKQAEDEQGNKLFDGAGKPLMKRCSMMKSLQLFNTDQCEGLPELMATQPV
QLMVQDEIEILSAPLMNRVVEILNHSGVKTTPRRQNRAFYRPLTDEIIMPLVGQFFTEADYWSTLLHELIHASGHQKRLN
REGITSSTRTFGDPIYAFEELIAELGSAFLCAELGIYGEVQHESYISGWLKRLRKDKKALFRACRQARQASEFLLTQQIG
NDG

Specific function: Required for autonomous replication in E.coli. Transferred into the recipient cell during bacterial conjugation. Catalyzes the synthesis of short oligoribonucleotide primers with CpA or pCpA at their 5'-termini on a single stranded template DNA [H]

COG id: COG4227

COG function: function code L; Antirestriction protein

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013610
- InterPro:   IPR006171 [H]

Pfam domain/function: PF08401 DUF1738; PF01751 Toprim [H]

EC number: NA

Molecular weight: Translated: 36668; Mature: 36668

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS00142 ZINC_PROTEASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKSTSCRKAHKRADLYQQVTDKIIVALEKGTIPWRKPWRSVRKPEGSSLPTNALTGRPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
QGVNIPLLWMAGEERGFGSDRWLTYRQAQKAGGQVRKGESCSLAVIFKPFEKQAEDEQGN
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCCHHCCC
KLFDGAGKPLMKRCSMMKSLQLFNTDQCEGLPELMATQPVQLMVQDEIEILSAPLMNRVV
EECCCCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EILNHSGVKTTPRRQNRAFYRPLTDEIIMPLVGQFFTEADYWSTLLHELIHASGHQKRLN
HHHHCCCCCCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
REGITSSTRTFGDPIYAFEELIAELGSAFLCAELGIYGEVQHESYISGWLKRLRKDKKAL
HCCCCCCHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FRACRQARQASEFLLTQQIGNDG
HHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKKSTSCRKAHKRADLYQQVTDKIIVALEKGTIPWRKPWRSVRKPEGSSLPTNALTGRPY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCC
QGVNIPLLWMAGEERGFGSDRWLTYRQAQKAGGQVRKGESCSLAVIFKPFEKQAEDEQGN
CCCCCEEEEEECCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCEEEEECCHHHHCCHHCCC
KLFDGAGKPLMKRCSMMKSLQLFNTDQCEGLPELMATQPVQLMVQDEIEILSAPLMNRVV
EECCCCCCHHHHHHHHHHHHHHCCCHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHH
EILNHSGVKTTPRRQNRAFYRPLTDEIIMPLVGQFFTEADYWSTLLHELIHASGHQKRLN
HHHHCCCCCCCCCHHCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHH
REGITSSTRTFGDPIYAFEELIAELGSAFLCAELGIYGEVQHESYISGWLKRLRKDKKAL
HCCCCCCHHHCCCHHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
FRACRQARQASEFLLTQQIGNDG
HHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1818755 [H]