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The map label for this gene is rob [H]

Identifier: 153946915

GI number: 153946915

Start: 2200012

End: 2200905

Strand: Reverse

Name: rob [H]

Synonym: YpsIP31758_1897

Alternate gene names: 153946915

Gene position: 2200905-2200012 (Counterclockwise)

Preceding gene: 153947931

Following gene: 153947680

Centisome position: 46.6

GC content: 43.18

Gene sequence:

>894_bases
ATGATGAGTGAAGACATATTGTTTATAGAAGAATTAATCGAGTGGATTGAAATAAATTTAGAAAAAAGGCCGACATTAGA
CGATGTGGCAAAGATCTCCGGATATTCAAAATGGCATTTACAACGGAAGTTTAAGAGTATCGTTGGGCTACAACTGGCAT
CCTATATCCGTGGGCGAGTGTTGACCCGAGCTGCGGTTGCGTTGCGTATATCAAGAAAGCCTATTATTACTATCTCTGAT
GAATTGGGATTTGACTCGCAACAGACCTTTACACGTACCTTTAAAAAACGGTTTGGCGTGACACCAAACAGCTTCCGCCA
GATGAACCATTGGGATGTTCAGGGGATGATCCCTCGCTTCGGATTCTACGAAAATTATACGCCGGAAATCAAGCAGGTAA
CGTTACCTGGAAAGGAATTAGTCGGTTTCACCCGGCGTCTGGATTTCGAAGAGTATAATGGTTGTGCTGTGCAGCGCTCT
TCCTGTATGGCGATGAAAGACGAGATCCTGCTTGATTTCTTTAAAGAGGTCAACTTTGCCTGTCAGCGGATCTACTCTTT
GTTTTCTGTTAAGGATGTTGATGGTCAACAGGGGCAGAAGAGCGTTTATTACTCAACGGCGATAGACAAAGAGAGAAAGC
ATGAGATTCAAGGGAGCCGAGAGATCGATCACATCGCGATTCCTGAAGGTGAATTTTTGTCTATCTCCCATCAGGGGAGC
GCTAAAGAGTGTGTAAAATTCGCAACTTACTTGTTTAACAACGTGTTACCAAAGTTACGTGATGAAGTTGGTCGTGGGAT
TGAGATGGAAGTCATTGAGATAGACCATTGTCATGCAGAATCGAAACTGCGTGATATTTCCGTAGTCTATCGTTATCTGA
TGGCAGTGAATTAA

Upstream 100 bases:

>100_bases
AAATGATTAATTTATATGAAGTATGAGAGAAAGAAAAGCATTGGAATAATGAGAAATTAATGTAGTATAAATTGTAAGTA
TATATATAAAGAATAATAAA

Downstream 100 bases:

>100_bases
CGCCCGATTAGCATCATTTAGCCATTATGGTTATTTAGCCAGAATGAGTATCCAGCCAGAATGATAGGTAATTGTGGCCG
AGCGTTTTCCCAAACTGAGA

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MMSEDILFIEELIEWIEINLEKRPTLDDVAKISGYSKWHLQRKFKSIVGLQLASYIRGRVLTRAAVALRISRKPIITISD
ELGFDSQQTFTRTFKKRFGVTPNSFRQMNHWDVQGMIPRFGFYENYTPEIKQVTLPGKELVGFTRRLDFEEYNGCAVQRS
SCMAMKDEILLDFFKEVNFACQRIYSLFSVKDVDGQQGQKSVYYSTAIDKERKHEIQGSREIDHIAIPEGEFLSISHQGS
AKECVKFATYLFNNVLPKLRDEVGRGIEMEVIEIDHCHAESKLRDISVVYRYLMAVN

Sequences:

>Translated_297_residues
MMSEDILFIEELIEWIEINLEKRPTLDDVAKISGYSKWHLQRKFKSIVGLQLASYIRGRVLTRAAVALRISRKPIITISD
ELGFDSQQTFTRTFKKRFGVTPNSFRQMNHWDVQGMIPRFGFYENYTPEIKQVTLPGKELVGFTRRLDFEEYNGCAVQRS
SCMAMKDEILLDFFKEVNFACQRIYSLFSVKDVDGQQGQKSVYYSTAIDKERKHEIQGSREIDHIAIPEGEFLSISHQGS
AKECVKFATYLFNNVLPKLRDEVGRGIEMEVIEIDHCHAESKLRDISVVYRYLMAVN
>Mature_297_residues
MMSEDILFIEELIEWIEINLEKRPTLDDVAKISGYSKWHLQRKFKSIVGLQLASYIRGRVLTRAAVALRISRKPIITISD
ELGFDSQQTFTRTFKKRFGVTPNSFRQMNHWDVQGMIPRFGFYENYTPEIKQVTLPGKELVGFTRRLDFEEYNGCAVQRS
SCMAMKDEILLDFFKEVNFACQRIYSLFSVKDVDGQQGQKSVYYSTAIDKERKHEIQGSREIDHIAIPEGEFLSISHQGS
AKECVKFATYLFNNVLPKLRDEVGRGIEMEVIEIDHCHAESKLRDISVVYRYLMAVN

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=265, Percent_Identity=32.0754716981132, Blast_Score=142, Evalue=3e-35,
Organism=Escherichia coli, GI87081928, Length=105, Percent_Identity=47.6190476190476, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1790497, Length=99, Percent_Identity=46.4646464646465, Blast_Score=98, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 34478; Mature: 34478

Theoretical pI: Translated: 7.53; Mature: 7.53

Prosite motif: PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMSEDILFIEELIEWIEINLEKRPTLDDVAKISGYSKWHLQRKFKSIVGLQLASYIRGRV
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTRAAVALRISRKPIITISDELGFDSQQTFTRTFKKRFGVTPNSFRQMNHWDVQGMIPRF
HHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCCCC
GFYENYTPEIKQVTLPGKELVGFTRRLDFEEYNGCAVQRSSCMAMKDEILLDFFKEVNFA
CCCCCCCCCCEEEECCHHHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CQRIYSLFSVKDVDGQQGQKSVYYSTAIDKERKHEIQGSREIDHIAIPEGEFLSISHQGS
HHHHHHHHHCCCCCCCCCCCEEEEHHHHCHHHHHHCCCCCCCCEEECCCCCEEEEECCCC
AKECVKFATYLFNNVLPKLRDEVGRGIEMEVIEIDHCHAESKLRDISVVYRYLMAVN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MMSEDILFIEELIEWIEINLEKRPTLDDVAKISGYSKWHLQRKFKSIVGLQLASYIRGRV
CCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LTRAAVALRISRKPIITISDELGFDSQQTFTRTFKKRFGVTPNSFRQMNHWDVQGMIPRF
HHHHHHHHEECCCCEEEEECCCCCCCHHHHHHHHHHHHCCCCHHHHHCCCCCHHCCCCCC
GFYENYTPEIKQVTLPGKELVGFTRRLDFEEYNGCAVQRSSCMAMKDEILLDFFKEVNFA
CCCCCCCCCCEEEECCHHHHHHHHHHCCCHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHH
CQRIYSLFSVKDVDGQQGQKSVYYSTAIDKERKHEIQGSREIDHIAIPEGEFLSISHQGS
HHHHHHHHHCCCCCCCCCCCEEEEHHHHCHHHHHHCCCCCCCCEEECCCCCEEEEECCCC
AKECVKFATYLFNNVLPKLRDEVGRGIEMEVIEIDHCHAESKLRDISVVYRYLMAVN
HHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]