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The map label for this gene is flp [H]

Identifier: 153937793

GI number: 153937793

Start: 730311

End: 731021

Strand: Direct

Name: flp [H]

Synonym: CLC_0710

Alternate gene names: 153937793

Gene position: 730311-731021 (Clockwise)

Preceding gene: 153934904

Following gene: 153937456

Centisome position: 19.42

GC content: 27.0

Gene sequence:

>711_bases
ATGAAAGAGTGTACTTGTGGAAGATGTCATCACAATATTTGTGCAAGGAAAGTTCCTATATTTTCTTCTTTATCAGAAGA
AGAGTTAATAAAAATTGTAAATATGACAGGGCATGAGGATTTTAAAAAGGGAGAAGCTATTTGCAATGAAGGAGATAAAT
CAGAAACTTTAATTATTATAAATGAAGGGAAAGTAAAACTTTGTAAGCTTACTAAAGAAGGAAAAGAACAAATTATTCAT
ATACTTTCTAGTGGAGATTTTTTTGGAGAACTAAATCTTTTTAATGATAATAAAAGCTATAATTTTTCCGCTTATGCTAT
ATCCCATGTAAAAATATGCACTCTTACAAAAAATAAGATGGACGAGATATTATTAAAAAATCCAGATATATCTTTAAAGA
TATTAAAAGAAGTTACTAAAAGACTTGCAGAAACGGAAACTTTAGCCCAAAATTTAGCTACCAATGATGCAGATATTAGA
ATAGCTAATATGATATTAGATTTTGGTGAAAAATATGGAGTAAATAAAGAACAATATATAGAAATAAAACTGCCTATAAA
TAGGGAAGAGATGGCAAACTATGTAGGGGTTACAAGAGAAACTATAAGTAGAAAATTATCTAAATTTGAGGATTTAGATA
TTATAAAACTCGTTGGTAATAAGGTAATTATAATTAAAGATGAAGATGCATTGAAAGAATTTATAGAATAG

Upstream 100 bases:

>100_bases
TTTATTTGTAGGGGGTAATATTATATAAATAATATATAAACAGAACGTATAGTTACAAATATAAATTAGTTTTTTATATT
AAATAAAGGGGATGCTTTTT

Downstream 100 bases:

>100_bases
ATTATTTTAGAGAAAAGTGCTTTGCACTTTTTTATTTTTTTTGAAAATTTGATTTGGATCATATTTTTAATTTTAAAGAA
TTTATATAATAAAACCATAA

Product: Crp/FNR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISHVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Sequences:

>Translated_236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISHVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
>Mature_236_residues
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIIINEGKVKLCKLTKEGKEQIIH
ILSSGDFFGELNLFNDNKSYNFSAYAISHVKICTLTKNKMDEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIR
IANMILDFGEKYGVNKEQYIEIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE

Specific function: This Protein Complexes With Cyclic AMP And Binds To Specific DNA Sites Near The Promoter To Regulate The Transcription Of Several Catabolite-Sensitive Operons. The Protein Induces A Severe Bend In The DNA. Acts As A Negative Regulator Of Its Own Synthesi

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=185, Percent_Identity=29.7297297297297, Blast_Score=94, Evalue=6e-21,
Organism=Escherichia coli, GI1787595, Length=213, Percent_Identity=25.3521126760563, Blast_Score=80, Evalue=8e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 26860; Mature: 26860

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
3.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIII
CCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEE
NEGKVKLCKLTKEGKEQIIHILSSGDFFGELNLFNDNKSYNFSAYAISHVKICTLTKNKM
ECCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCHH
DEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIRIANMILDFGEKYGVNKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC
>Mature Secondary Structure
MKECTCGRCHHNICARKVPIFSSLSEEELIKIVNMTGHEDFKKGEAICNEGDKSETLIII
CCCCCCCHHHHHHHHHCCCHHHCCCHHHHHHHHCCCCCCHHHHHHHHHCCCCCCCEEEEE
NEGKVKLCKLTKEGKEQIIHILSSGDFFGELNLFNDNKSYNFSAYAISHVKICTLTKNKM
ECCCEEEEECCHHHHHHHHHHHCCCCCEEEEEEECCCCCCCEEEEEEEEEEEEEECCCHH
DEILLKNPDISLKILKEVTKRLAETETLAQNLATNDADIRIANMILDFGEKYGVNKEQYI
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEHHHHHHHHHHHHCCCCHHEE
EIKLPINREEMANYVGVTRETISRKLSKFEDLDIIKLVGNKVIIIKDEDALKEFIE
EEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEECHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2113923; 8441692 [H]