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The map label for this gene is nth [H]

Identifier: 153937471

GI number: 153937471

Start: 240104

End: 240745

Strand: Direct

Name: nth [H]

Synonym: CLC_0265

Alternate gene names: 153937471

Gene position: 240104-240745 (Clockwise)

Preceding gene: 153935872

Following gene: 153937822

Centisome position: 6.38

GC content: 29.13

Gene sequence:

>642_bases
TTGAATAATCATGAAATAAAAAATGTTATAGATATATTAGTAGACACATATCCTGATGCTAATTGTGAATTAGAACACAG
AAATCCTTTTGAACTGTTAATAGCTACAGTCTTGTCTGCTCAAACTACAGATAAAAAAGTAAATGAGATAACAAAGGAAC
TATTTAAAGAATATTCTACTCCTAAGGATTTCTTAAAATTAACAAGGGAAGAATTAGAAGAGAAAATTAAAAAAATAGGT
TTATATAGAAATAAATCTAAGAATATATTATTGCTTTGTAAAGAGTTAGAAGAGAAGTTTGGAAGCCAAGTTCCTAATGA
TTTCAATGATCTAACTTCACTACCAGGAGTAGGTAGAAAAACTGCTAACGTGGTTTTAGCCAATGCTTTTAAAGTACCTA
CTATAGCAGTAGATACCCATGTTTTTAGAGTATCTAACAGAATAGGATTAGTAGATGCTAGTAATGTACTAAAAACGGAA
GAGCAGTTGCAGCAAGCTATACCAAAGGAACTATGGATATTAATGCATCATGTGTTAATATTTCATGGAAGAAGATGTTG
CGTAGCTAGAAAACCTAAATGTGAAGAATGTACTATAAAAAAATATTGCAAATATTATAATGAAGAAATTAAACCATCTT
AA

Upstream 100 bases:

>100_bases
ATAATTAAAACTTGTTTTATTTGGAAGTTTAATAAGAACTAAGGAATACTTTAATAGATGCAGTCATTTATTAAACTAAA
ATTATGGGAGTGATTTTCAA

Downstream 100 bases:

>100_bases
GTATAAGTGTTTACTTATAATAGCAAATTATCTTATATCTAAGATGGTTTTGCTATTAAAGATATCCACATGTGGATAAT
TTTTTTTAGATTTTGTTTAT

Product: endonuclease III

Products: NA

Alternate protein names: DNA-(apurinic or apyrimidinic site) lyase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MNNHEIKNVIDILVDTYPDANCELEHRNPFELLIATVLSAQTTDKKVNEITKELFKEYSTPKDFLKLTREELEEKIKKIG
LYRNKSKNILLLCKELEEKFGSQVPNDFNDLTSLPGVGRKTANVVLANAFKVPTIAVDTHVFRVSNRIGLVDASNVLKTE
EQLQQAIPKELWILMHHVLIFHGRRCCVARKPKCEECTIKKYCKYYNEEIKPS

Sequences:

>Translated_213_residues
MNNHEIKNVIDILVDTYPDANCELEHRNPFELLIATVLSAQTTDKKVNEITKELFKEYSTPKDFLKLTREELEEKIKKIG
LYRNKSKNILLLCKELEEKFGSQVPNDFNDLTSLPGVGRKTANVVLANAFKVPTIAVDTHVFRVSNRIGLVDASNVLKTE
EQLQQAIPKELWILMHHVLIFHGRRCCVARKPKCEECTIKKYCKYYNEEIKPS
>Mature_213_residues
MNNHEIKNVIDILVDTYPDANCELEHRNPFELLIATVLSAQTTDKKVNEITKELFKEYSTPKDFLKLTREELEEKIKKIG
LYRNKSKNILLLCKELEEKFGSQVPNDFNDLTSLPGVGRKTANVVLANAFKVPTIAVDTHVFRVSNRIGLVDASNVLKTE
EQLQQAIPKELWILMHHVLIFHGRRCCVARKPKCEECTIKKYCKYYNEEIKPS

Specific function: Has Both An Apurinic And/Or Apyrimidinic Endonuclease Activity And A DNA N-Glycosylase Activity. Incises Damaged DNA At Cytosines, Thymines And Guanines. Acts On A Damaged Strand, 5' From The Damaged Site. Required For The Repair Of Both Oxidative DNA Da

COG id: COG0177

COG function: function code L; Predicted EndoIII-related endonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Nth/MutY family [H]

Homologues:

Organism=Homo sapiens, GI4505471, Length=210, Percent_Identity=28.5714285714286, Blast_Score=99, Evalue=4e-21,
Organism=Escherichia coli, GI1787920, Length=205, Percent_Identity=46.8292682926829, Blast_Score=186, Evalue=1e-48,
Organism=Caenorhabditis elegans, GI17554540, Length=180, Percent_Identity=34.4444444444444, Blast_Score=106, Evalue=7e-24,
Organism=Saccharomyces cerevisiae, GI6319304, Length=177, Percent_Identity=32.7683615819209, Blast_Score=85, Evalue=9e-18,
Organism=Saccharomyces cerevisiae, GI6324530, Length=194, Percent_Identity=28.8659793814433, Blast_Score=84, Evalue=1e-17,
Organism=Drosophila melanogaster, GI45550361, Length=187, Percent_Identity=35.2941176470588, Blast_Score=112, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011257
- InterPro:   IPR004036
- InterPro:   IPR005759
- InterPro:   IPR004035
- InterPro:   IPR003651
- InterPro:   IPR003265
- InterPro:   IPR000445
- InterPro:   IPR003583
- InterPro:   IPR023170 [H]

Pfam domain/function: PF00633 HHH; PF00730 HhH-GPD [H]

EC number: =4.2.99.18 [H]

Molecular weight: Translated: 24574; Mature: 24574

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: PS00764 ENDONUCLEASE_III_1 ; PS01155 ENDONUCLEASE_III_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.3 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
3.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNHEIKNVIDILVDTYPDANCELEHRNPFELLIATVLSAQTTDKKVNEITKELFKEYST
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PKDFLKLTREELEEKIKKIGLYRNKSKNILLLCKELEEKFGSQVPNDFNDLTSLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCH
TANVVLANAFKVPTIAVDTHVFRVSNRIGLVDASNVLKTEEQLQQAIPKELWILMHHVLI
HHHHHHCCCCCCCEEEEHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHGRRCCVARKPKCEECTIKKYCKYYNEEIKPS
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MNNHEIKNVIDILVDTYPDANCELEHRNPFELLIATVLSAQTTDKKVNEITKELFKEYST
CCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCC
PKDFLKLTREELEEKIKKIGLYRNKSKNILLLCKELEEKFGSQVPNDFNDLTSLPGVGRK
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEHHHHHHHHCCCCCCCHHHHHCCCCCCCH
TANVVLANAFKVPTIAVDTHVFRVSNRIGLVDASNVLKTEEQLQQAIPKELWILMHHVLI
HHHHHHCCCCCCCEEEEHHHHHHHHCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHHHHH
FHGRRCCVARKPKCEECTIKKYCKYYNEEIKPS
HCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7704260; 8760912; 9384377 [H]