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The map label for this gene is ruvB

Identifier: 153937310

GI number: 153937310

Start: 3127095

End: 3128123

Strand: Reverse

Name: ruvB

Synonym: CLC_2972

Alternate gene names: 153937310

Gene position: 3128123-3127095 (Counterclockwise)

Preceding gene: 153936604

Following gene: 153935623

Centisome position: 83.18

GC content: 31.97

Gene sequence:

>1029_bases
ATGGAGAATAGAATGGTAACTCCTTTTGATGTAGAAGATGATAGGGAACAGTATAGTTTAAGACCTACTACATTAAAAGA
ATACATAGGTCAAAAAAAAGTAAAAGCCAATTTAGATATATTTATAAAAGCAGCTAAAAAAAGGAACGAGTCTTTAGATC
ATGTGCTTTTTTATGGGCCACCTGGACTAGGTAAAACTACCCTTGCTAATATCATAGCTAATGAAATGACAGGTAATTTA
AAAGTAACTTCAGGTCCAGCTATAGAAAAAGCAGGGGATTTAGCAGCTATATTAACTAGCTTAACAGATTATGATGTTTT
ATTTATAGATGAAATTCATAGATTAAATAGATCTATAGAAGAAATATTATATCCAGCTATGGAAGATTATGCTTTAGATA
TAGTTATAGGCAAGGGTGCTGCAGCAAAGTCTATAAGATTAGATCTTCCTAAGTTTACTCTTATAGGAGCTACTACAAGG
GTAGGGCTGTTAACATCTCCTTTAAGAGATAGATTTGGTATGTTATGTGCTATGGAGTTTTATACAGACGAGGAACTTAT
GGAGATAGTAGTAAGATCAGCAGCTATATTGAATGTGAATATTTGTAGAGAAGCTGCTTTTGAAATAGGAAAAAGATCTA
GGGGTACTCCAAGAATTGCTAATAGGCTTTTAAAAAGAGTTAGAGATTATTGTGATGTTAAACATGATGGTGATATAGAC
TTACAGGGAGCAAAGGCAGCTCTGGACTTATTGGAAGTAGATAAGGAAGGTTTAGATAAAATAGACAATAAAATTTTAGA
AGCTATAATTTTTAATTTTAAAGGAGGACCTGTTGGATTAGAAACATTAGCTTATTTTATAGGGGAGGAACTAGATACCA
TAGAGGATGTTTATGAACCATATCTTATACAAAAGGGATTTATAATGAGAACTCCAAGAGGAAGAGTGGCCTCAGAAAAA
GCATATAACCATTTTGGAGTAACAAAAAAAGAAGAAAAGGATAATCAAGTATCTATATTTAATAAATAA

Upstream 100 bases:

>100_bases
AATAAAGTGATTAATTCATGTGATAAAAATAATTCTTTAGAACAGATTATAAAGGAAGCTTTAAAATATTTAATGAAATA
AATTAGAGGGGGGTACTTAT

Downstream 100 bases:

>100_bases
TAAATTTTAATTCTATATTAAGAGGTGGAAGACGTGAAAGTAAAAGATTTTGATTTTTATTTACCAGAGGAATTAATAGC
ACAACATCCTATAGAGAAAA

Product: Holliday junction DNA helicase RuvB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRNESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK

Sequences:

>Translated_342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRNESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK
>Mature_342_residues
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRNESLDHVLFYGPPGLGKTTLANIIANEMTGNL
KVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIEEILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTR
VGLLTSPLRDRFGMLCAMEFYTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEPYLIQKGFIMRTPRGRVASEK
AYNHFGVTKKEEKDNQVSIFNK

Specific function: The ruvA-ruvB complex in the presence of ATP renatures cruciform structure in supercoiled DNA with palindromic sequence, indicating that it may promote strand exchange reactions in homologous recombination. RuvAB is an helicase that mediates the Holliday

COG id: COG2255

COG function: function code L; Holliday junction resolvasome, helicase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvB family

Homologues:

Organism=Escherichia coli, GI1788167, Length=324, Percent_Identity=61.7283950617284, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1787119, Length=152, Percent_Identity=32.2368421052632, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUVB_CLOB1 (A7FY19)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001385392.1
- ProteinModelPortal:   A7FY19
- SMR:   A7FY19
- STRING:   A7FY19
- GeneID:   5397983
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_3099
- eggNOG:   COG2255
- HOGENOM:   HBG339960
- OMA:   EKFDGGP
- ProtClustDB:   PRK00080
- BioCyc:   CBOT441770:CLB_3099-MONOMER
- HAMAP:   MF_00016
- InterPro:   IPR003593
- InterPro:   IPR004605
- InterPro:   IPR008823
- InterPro:   IPR008824
- InterPro:   IPR011991
- Gene3D:   G3DSA:1.10.10.10
- ProDom:   PD005323
- SMART:   SM00382
- TIGRFAMs:   TIGR00635

Pfam domain/function: PF05491 RuvB_C; PF05496 RuvB_N

EC number: =3.6.4.12

Molecular weight: Translated: 38401; Mature: 38401

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRNESLDHVLFYGP
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCHHHCCCHHEEEEEHHHCCCCCCEEEEECC
PGLGKTTLANIIANEMTGNLKVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIE
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTRVGLLTSPLRDRFGMLCAMEF
HHHHHHHHCEEEEEEEECCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHH
YTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEP
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
YLIQKGFIMRTPRGRVASEKAYNHFGVTKKEEKDNQVSIFNK
HHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEECCC
>Mature Secondary Structure
MENRMVTPFDVEDDREQYSLRPTTLKEYIGQKKVKANLDIFIKAAKKRNESLDHVLFYGP
CCCCCCCCCCCCCCHHHHCCCCHHHHHHHCCHHHCCCHHEEEEEHHHCCCCCCEEEEECC
PGLGKTTLANIIANEMTGNLKVTSGPAIEKAGDLAAILTSLTDYDVLFIDEIHRLNRSIE
CCCCHHHHHHHHHHHCCCCEEECCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHH
EILYPAMEDYALDIVIGKGAAAKSIRLDLPKFTLIGATTRVGLLTSPLRDRFGMLCAMEF
HHHHHHHHCEEEEEEEECCCCCCEEEECCCCEEEEECHHHHHHHHHHHHHHHHHEEHHHH
YTDEELMEIVVRSAAILNVNICREAAFEIGKRSRGTPRIANRLLKRVRDYCDVKHDGDID
CCHHHHHHHHHHHHHHEEHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
LQGAKAALDLLEVDKEGLDKIDNKILEAIIFNFKGGPVGLETLAYFIGEELDTIEDVYEP
CCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCHHHHHHHHHHH
YLIQKGFIMRTPRGRVASEKAYNHFGVTKKEEKDNQVSIFNK
HHHCCCCEEECCCCCCHHHHHHHHCCCCCCCCCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA