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The map label for this gene is ascB [H]

Identifier: 153937231

GI number: 153937231

Start: 959089

End: 960510

Strand: Direct

Name: ascB [H]

Synonym: CLC_0935

Alternate gene names: 153937231

Gene position: 959089-960510 (Clockwise)

Preceding gene: 153936097

Following gene: 153937029

Centisome position: 25.5

GC content: 32.42

Gene sequence:

>1422_bases
ATGAGTGGATTAGCAAAGGATTTCTTGTGGGGCGGAGCTACGGCAGCTAACCAATGCGAAGGTGGTTATTTAGAAGGAAA
TAGGGGATTAGGAACTGTGGATGTTATTCCAGCAGGAGTAAATCGTAGAGCTGTTATGCAAGGAAAGATGCATTATAATA
ATTTACAAGAGGATTCTTATTTCCCATCACATGAAGCAATAGATATGTATCATAACTACAAGGAGGATATAGCATTATTT
GCAGAAATGGGATTTAAATGTTATCGTTTCTCACTTTCATGGTCAAGAATTTTCCCAACAGGAAAGGAACAAGAACCAAA
TGAAGAAGGCTTAGCATTTTATGAAAAATTTATTGATGAGCTATTAAAATATAATATTGAACCAGTAGTTACCATATGCC
ATTTTGATGTACCTCTTGTATTGGAGCAGGAATATGGTTCATGGAGAAGTAGAAAATTAATAGATGCATATCTTAGATAT
TGTGAAGCAATATTTAAAAGATTTAATGGGAAAGTAAAGTATTGGATAACTTTTAATGAAATAAATATGCTTATGCATTT
ACCCTTTATGGGAGCTGGAATAATGTTTAAGGAAGGGGAAAATGAGCTAGAAGTAAAATATCAAGCAGCTCATAATGAAT
TAGTGGCATCAGCCTTAGCTACAAAATTAGCAAAGGAAATTAATAAAGATAATAAAGTAGGATGTATGTTAGCGGCAGGG
CAATTCTATCCATATACTTGTAAGCCAGAAGATGTTCTAGAAGCTATGGAGAAAGATAAAAATAATTATTTCTTCATAGA
TGTACAATCTAGGGGAGAATACCCGAGCTATGCTAAAAAATTCTTAGAGAGAAACAATATAAAAATAGAAATGCAGTCAG
AAGATGAGAAGATTTTAAGGGAGAATACTGTTGATTTTATTGCATTTAGTTACTATACTTCAAGACTTGCAAGTAGTGAC
CCGAATGCAGGAGAAAAATCAGCTGGTAATGTAATAACATCATTAAAAAATCCACATTTAAAATCTAGTAAATGGGGATG
GCAAATAGATCCAATAGGCCTTAGAATTACAATGAATTCACTTTATGATCGTTATCAAAAACCGCTATTCATTGTGGAAA
ATGGCTTAGGTGCTGTTGATATTTTAAATGAGGATGGGACTGTAGATGATGATTATAGAATTGCTTATCTTGAAACTCAT
ATAAAAAATATGATTGATGCAGTTGAATTAGATGGTGTTGAACTATTAGGATATACAACTTGGGGATGCATTGATTTAGT
TAGTGCATCAACAGGGGAAATGAAAAAGCGATATGGTTTTATATATGTAGATAAGGATAATGATGGTCATGGAACTTTAA
AGAGATACAAGAAAAAATCTTTCTATTGGTACAAAAATGTTATTAAAAATAATGGACTATAA

Upstream 100 bases:

>100_bases
AGCTTATTCATATGCTTATTACATATATTTTTTATATTTACTATATTAAAATTAAAGCTATTTTGAGACAACACCTTATT
TAATGAAAGGAAGGTAAAAT

Downstream 100 bases:

>100_bases
ATAATTGAAAAAATATATAATATATAAATTTGTAATAGCAATAGGCCAAAAATAAGGGAAGATTAGGAATGTAAAATATT
TTAGGATTTACTGAGGCATA

Product: glycosy hydrolase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 473; Mature: 472

Protein sequence:

>473_residues
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALF
AEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGENELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Sequences:

>Translated_473_residues
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALF
AEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRY
CEAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGENELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSD
PNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETH
IKNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
>Mature_472_residues
SGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSYFPSHEAIDMYHNYKEDIALFA
EMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDELLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYC
EAIFKRFNGKVKYWITFNEINMLMHLPFMGAGIMFKEGENELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAGQ
FYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILRENTVDFIAFSYYTSRLASSDP
NAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNSLYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHI
KNMIDAVELDGVELLGYTTWGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL

Specific function: Can hydrolyze salicin, cellobiose, and probably arbutin [H]

COG id: COG2723

COG function: function code G; Beta-glucosidase/6-phospho-beta-glucosidase/beta-galactosidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 1 family [H]

Homologues:

Organism=Homo sapiens, GI32481206, Length=489, Percent_Identity=28.8343558282209, Blast_Score=182, Evalue=9e-46,
Organism=Homo sapiens, GI13273313, Length=485, Percent_Identity=29.0721649484536, Blast_Score=177, Evalue=1e-44,
Organism=Homo sapiens, GI110681710, Length=492, Percent_Identity=29.2682926829268, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI24497614, Length=480, Percent_Identity=28.125, Blast_Score=152, Evalue=7e-37,
Organism=Homo sapiens, GI28376633, Length=483, Percent_Identity=25.0517598343685, Blast_Score=149, Evalue=8e-36,
Organism=Escherichia coli, GI1789070, Length=472, Percent_Identity=60.3813559322034, Blast_Score=622, Evalue=1e-179,
Organism=Escherichia coli, GI2367174, Length=480, Percent_Identity=52.0833333333333, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI2367270, Length=473, Percent_Identity=51.3742071881607, Blast_Score=508, Evalue=1e-145,
Organism=Caenorhabditis elegans, GI17552856, Length=491, Percent_Identity=30.5498981670061, Blast_Score=170, Evalue=1e-42,
Organism=Caenorhabditis elegans, GI17539390, Length=491, Percent_Identity=28.5132382892057, Blast_Score=157, Evalue=9e-39,
Organism=Drosophila melanogaster, GI21356577, Length=470, Percent_Identity=25.7446808510638, Blast_Score=141, Evalue=1e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001360
- InterPro:   IPR018120
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00232 Glyco_hydro_1 [H]

EC number: =3.2.1.86 [H]

Molecular weight: Translated: 54340; Mature: 54209

Theoretical pI: Translated: 5.56; Mature: 5.56

Prosite motif: PS00572 GLYCOSYL_HYDROL_F1_1 ; PS00653 GLYCOSYL_HYDROL_F1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHNYKEDIALFAEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCCHHHHHHHCHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWITFNE
HHHCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
INMLMHLPFMGAGIMFKEGENELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
EEEEEECCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SGLAKDFLWGGATAANQCEGGYLEGNRGLGTVDVIPAGVNRRAVMQGKMHYNNLQEDSY
CCCHHHHHCCCCCCCCCCCCCEEECCCCCCEEEEEECCCCCCEEEECCHHCCCCCCCCC
FPSHEAIDMYHNYKEDIALFAEMGFKCYRFSLSWSRIFPTGKEQEPNEEGLAFYEKFIDE
CCCCHHHHHHHCHHHHHHHHHHCCCEEEEEEEEHHHCCCCCCCCCCCHHHHHHHHHHHHH
LLKYNIEPVVTICHFDVPLVLEQEYGSWRSRKLIDAYLRYCEAIFKRFNGKVKYWITFNE
HHHCCCCEEEEEEECCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
INMLMHLPFMGAGIMFKEGENELEVKYQAAHNELVASALATKLAKEINKDNKVGCMLAAG
EEEEEECCCCCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEC
QFYPYTCKPEDVLEAMEKDKNNYFFIDVQSRGEYPSYAKKFLERNNIKIEMQSEDEKILR
CCCCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCEEEECCCHHHHHH
ENTVDFIAFSYYTSRLASSDPNAGEKSAGNVITSLKNPHLKSSKWGWQIDPIGLRITMNS
HCCHHHEEHHHHHHHHCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCEEECCEEEEEEHHH
LYDRYQKPLFIVENGLGAVDILNEDGTVDDDYRIAYLETHIKNMIDAVELDGVELLGYTT
HHHHHCCCEEEEECCCCEEEEECCCCCCCCCCEEEHHHHHHHHHHHHHHCCCEEEEEECH
WGCIDLVSASTGEMKKRYGFIYVDKDNDGHGTLKRYKKKSFYWYKNVIKNNGL
HHHHHHHCCCCCHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1630307; 9278503 [H]