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The map label for this gene is dagK [H]

Identifier: 153937206

GI number: 153937206

Start: 229870

End: 230754

Strand: Direct

Name: dagK [H]

Synonym: CLC_0256

Alternate gene names: 153937206

Gene position: 229870-230754 (Clockwise)

Preceding gene: 153936233

Following gene: 153935045

Centisome position: 6.11

GC content: 30.06

Gene sequence:

>885_bases
ATGAGTAAGGTAAAATTTATATATAACCCATATTCAGGAGAAAAGGCTATAATATCTCAAATAGACAAAGTAATAAGAAT
ACATCAGAAATATGGATATGAAGTAGTGCCTTATAGGATAAGTTTAGAATATGATCTTGATGATGCTTTTAAATCTATTG
ATGAAAGCTATAAATATATATTAGTAGCAGGTGGAGATGGTACTGTAGACAATGTGGTAAATCATTTAAAAAAATTTTCA
ATTGATCTTCCAATTGCAATATTACCTACAGGTACTGCTAATGATTTTGCTAAATTTATTGGTATGCCAACAAATGTTGG
AGAGGCCTGTGAGCAAATACTAAAGAGCTCTGTAAAAAAGATTGATTTAGGAAAAATAAATGATAAATATTTTATTAATG
TTGCTAGCACAGGACTATTTACAGATGTATCTCAAAAAACCGATGTTAATTTAAAGAATACTATGGGAAAACTTGCCTAC
TATGTAAAGGGATTAGAGCAGCTTCCTAATTTCAGAAGATTAAAGGTTAATGTTAGATCTGAGGAAGTTAATTTTGATGG
AGATATGTATCTTATGATGGTGTTCAATGGACAAACAGCAGGCAATCTTCATATGGCTTATAAGGCAGAAGTTGATGATG
GCATGTTAGATGTAATAATAATAAAGGCTTGTGCTATTAAGGATATGATAGGATTATTTATAAAAATGATAAGAGGAGAT
CATCTAGAAGATGTAAATGCTCTAATATATTTTAAAACAAATAAACTGGAAATAGAATCTAATGAAGAGATAGTTACGGA
TATAGATGGAGAAAGAGGTCCAGACTTTCCTTTGCAGATAGAATGTGTAAAAGAAGGTTTGGATATTTTGGGCATATTAG
ATTAA

Upstream 100 bases:

>100_bases
AATATCCACAGGATTTATTGTTAAAATCTTGTGGATTTGCTATTATATAATGGTACAGCTAATTTAATTATAAAGTAAAA
ACTATAAGAGGAGATTTGAA

Downstream 100 bases:

>100_bases
TGTAGGAATATTTTAATTTTAATCTTAATAAATATAAGTAGTAGCATTGTTAAATTGGAGAGTTTATTTAATTATGATAA
ATTTATTTATTTATATATCA

Product: putative lipid kinase

Products: NA

Alternate protein names: DAG kinase; DAGK [H]

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MSKVKFIYNPYSGEKAIISQIDKVIRIHQKYGYEVVPYRISLEYDLDDAFKSIDESYKYILVAGGDGTVDNVVNHLKKFS
IDLPIAILPTGTANDFAKFIGMPTNVGEACEQILKSSVKKIDLGKINDKYFINVASTGLFTDVSQKTDVNLKNTMGKLAY
YVKGLEQLPNFRRLKVNVRSEEVNFDGDMYLMMVFNGQTAGNLHMAYKAEVDDGMLDVIIIKACAIKDMIGLFIKMIRGD
HLEDVNALIYFKTNKLEIESNEEIVTDIDGERGPDFPLQIECVKEGLDILGILD

Sequences:

>Translated_294_residues
MSKVKFIYNPYSGEKAIISQIDKVIRIHQKYGYEVVPYRISLEYDLDDAFKSIDESYKYILVAGGDGTVDNVVNHLKKFS
IDLPIAILPTGTANDFAKFIGMPTNVGEACEQILKSSVKKIDLGKINDKYFINVASTGLFTDVSQKTDVNLKNTMGKLAY
YVKGLEQLPNFRRLKVNVRSEEVNFDGDMYLMMVFNGQTAGNLHMAYKAEVDDGMLDVIIIKACAIKDMIGLFIKMIRGD
HLEDVNALIYFKTNKLEIESNEEIVTDIDGERGPDFPLQIECVKEGLDILGILD
>Mature_293_residues
SKVKFIYNPYSGEKAIISQIDKVIRIHQKYGYEVVPYRISLEYDLDDAFKSIDESYKYILVAGGDGTVDNVVNHLKKFSI
DLPIAILPTGTANDFAKFIGMPTNVGEACEQILKSSVKKIDLGKINDKYFINVASTGLFTDVSQKTDVNLKNTMGKLAYY
VKGLEQLPNFRRLKVNVRSEEVNFDGDMYLMMVFNGQTAGNLHMAYKAEVDDGMLDVIIIKACAIKDMIGLFIKMIRGDH
LEDVNALIYFKTNKLEIESNEEIVTDIDGERGPDFPLQIECVKEGLDILGILD

Specific function: Catalyzes the phosphorylation of diacylglycerol (DAG) into phosphatidic acid. Is a key enzyme involved in the production of lipoteichoic acid by reintroducing DAG formed from the breakdown of membrane phospholipids into the phosphatidylglycerol biosynthet

COG id: COG1597

COG function: function code IR; Sphingosine kinase and enzymes related to eukaryotic diacylglycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 DAGKc domain [H]

Homologues:

Organism=Escherichia coli, GI1788402, Length=257, Percent_Identity=26.4591439688716, Blast_Score=105, Evalue=4e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017438
- InterPro:   IPR005218
- InterPro:   IPR001206 [H]

Pfam domain/function: PF00781 DAGK_cat [H]

EC number: =2.7.1.107 [H]

Molecular weight: Translated: 33053; Mature: 32921

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKVKFIYNPYSGEKAIISQIDKVIRIHQKYGYEVVPYRISLEYDLDDAFKSIDESYKYI
CCCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHCCCCEEE
LVAGGDGTVDNVVNHLKKFSIDLPIAILPTGTANDFAKFIGMPTNVGEACEQILKSSVKK
EEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHE
IDLGKINDKYFINVASTGLFTDVSQKTDVNLKNTMGKLAYYVKGLEQLPNFRRLKVNVRS
ECCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
EEVNFDGDMYLMMVFNGQTAGNLHMAYKAEVDDGMLDVIIIKACAIKDMIGLFIKMIRGD
CEECCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HLEDVNALIYFKTNKLEIESNEEIVTDIDGERGPDFPLQIECVKEGLDILGILD
CCCCCCEEEEEECCEEEECCCCCEEEECCCCCCCCCCEEEHHHHCCCCEEEECC
>Mature Secondary Structure 
SKVKFIYNPYSGEKAIISQIDKVIRIHQKYGYEVVPYRISLEYDLDDAFKSIDESYKYI
CCEEEEECCCCCCHHHHHHHHHHHHHHHHCCCEEEEEEEEEEECHHHHHHHHCCCCEEE
LVAGGDGTVDNVVNHLKKFSIDLPIAILPTGTANDFAKFIGMPTNVGEACEQILKSSVKK
EEECCCCCHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHE
IDLGKINDKYFINVASTGLFTDVSQKTDVNLKNTMGKLAYYVKGLEQLPNFRRLKVNVRS
ECCCCCCCEEEEEEECCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEEC
EEVNFDGDMYLMMVFNGQTAGNLHMAYKAEVDDGMLDVIIIKACAIKDMIGLFIKMIRGD
CEECCCCCEEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
HLEDVNALIYFKTNKLEIESNEEIVTDIDGERGPDFPLQIECVKEGLDILGILD
CCCCCCEEEEEECCEEEECCCCCEEEECCCCCCCCCCEEEHHHHCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]