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The map label for this gene is fba [H]

Identifier: 153937182

GI number: 153937182

Start: 224266

End: 225192

Strand: Reverse

Name: fba [H]

Synonym: CLC_0249

Alternate gene names: 153937182

Gene position: 225192-224266 (Counterclockwise)

Preceding gene: 153936874

Following gene: 153935232

Centisome position: 5.99

GC content: 34.63

Gene sequence:

>927_bases
ATGGCATTAGTAACGACTAAAGAAATGTTTAAAAAAGCTTATGAAGGTAAATATGCTATAGGAGCATTCAACATTAACAA
TATGGAAATATTACAAGGCGTTGTAAACTCTGCTAAGGCTGCAAACTCAGCTGTTATACTTCAAGTATCTGCAGGGGCTT
TAAAATATGCTGGTCCTAAATACTTAAAAGCAATGGTAGATGCAGCTATAGCTGATACTGGAATAGATGTAGCTCTACAT
TTAGATCATGGTGCAAGCTTAGATGTTGTAAAATTAGCTGTAGAAAGCGGATTTACTTCTGTAATGTTTGACGGCTCTCA
TTATGATTATGAAGAAAATGTAGCTAAAACTAAAGAAGTTGTAGAATATGCTCACTCTCATGGTGTTGTAGTTGAAGCTG
AATTAGGAGTATTAGCAGGAGTAGAAGATGATGTGCAAAGTGATGTACATATCTACACTGATCCAGAACAAGCTGTAGAC
TTCGTAAATAGAACAGGTGTTGATTCTTTAGCAATAGCTATAGGAACAAGCCATGGTGCTTACAAATTCGCCGGAGAAGC
TCAATTAAGATTTGATATATTAGAAGAAATCCAATCAAAATTACCTGGATATCCAATAGTTCTTCACGGAGCATCTTCTG
TAGATCCAGAATCTGTTGATACTTGTAACAAATACGGTGGAGAAATAAAAGGAGCTAAAGGGGTTCCAGCTGATATGCTT
AGAAAAGCATCTTCAATGGCAGTATGTAAAATAAACATGGATACAGATTTAAGATTAGCTATGACAGCTGGTATAAGAAA
AGTACTTTCTGAAGATCCAAAACAATTTGACCCAAGAAAATACTTAGGTGAAGGTAGATCAAGAATAGAAAAAATCGTTG
AAGATAAAATAAAAAATGTTTTAGGTTCAGCTGATTCTTTAAAATAG

Upstream 100 bases:

>100_bases
ATTTATAATATAATTATAGTTAATAAATAAAAGTCCCATAGGGACTTAATGCGTCCCTATGAATTTATTTTTAAATTTAT
TTTGGAAGGTGGAATTTTGA

Downstream 100 bases:

>100_bases
ATCTCATAAAAAGCTATACTAATATTTGATATAATAGTAGATAGATTTTTCTGTTAAGAATAAATCTATCTACTTTTTTT
TGATATTAACTTATTTGATG

Product: fructose-1,6-bisphosphate aldolase, class II

Products: NA

Alternate protein names: FBP aldolase; FBPA; Fructose-1,6-bisphosphate aldolase [H]

Number of amino acids: Translated: 308; Mature: 307

Protein sequence:

>308_residues
MALVTTKEMFKKAYEGKYAIGAFNINNMEILQGVVNSAKAANSAVILQVSAGALKYAGPKYLKAMVDAAIADTGIDVALH
LDHGASLDVVKLAVESGFTSVMFDGSHYDYEENVAKTKEVVEYAHSHGVVVEAELGVLAGVEDDVQSDVHIYTDPEQAVD
FVNRTGVDSLAIAIGTSHGAYKFAGEAQLRFDILEEIQSKLPGYPIVLHGASSVDPESVDTCNKYGGEIKGAKGVPADML
RKASSMAVCKINMDTDLRLAMTAGIRKVLSEDPKQFDPRKYLGEGRSRIEKIVEDKIKNVLGSADSLK

Sequences:

>Translated_308_residues
MALVTTKEMFKKAYEGKYAIGAFNINNMEILQGVVNSAKAANSAVILQVSAGALKYAGPKYLKAMVDAAIADTGIDVALH
LDHGASLDVVKLAVESGFTSVMFDGSHYDYEENVAKTKEVVEYAHSHGVVVEAELGVLAGVEDDVQSDVHIYTDPEQAVD
FVNRTGVDSLAIAIGTSHGAYKFAGEAQLRFDILEEIQSKLPGYPIVLHGASSVDPESVDTCNKYGGEIKGAKGVPADML
RKASSMAVCKINMDTDLRLAMTAGIRKVLSEDPKQFDPRKYLGEGRSRIEKIVEDKIKNVLGSADSLK
>Mature_307_residues
ALVTTKEMFKKAYEGKYAIGAFNINNMEILQGVVNSAKAANSAVILQVSAGALKYAGPKYLKAMVDAAIADTGIDVALHL
DHGASLDVVKLAVESGFTSVMFDGSHYDYEENVAKTKEVVEYAHSHGVVVEAELGVLAGVEDDVQSDVHIYTDPEQAVDF
VNRTGVDSLAIAIGTSHGAYKFAGEAQLRFDILEEIQSKLPGYPIVLHGASSVDPESVDTCNKYGGEIKGAKGVPADMLR
KASSMAVCKINMDTDLRLAMTAGIRKVLSEDPKQFDPRKYLGEGRSRIEKIVEDKIKNVLGSADSLK

Specific function: Catalyzes the aldol condensation of dihydroxyacetone phosphate (DHAP or glycerone-phosphate) with glyceraldehyde 3- phosphate (G3P) to form fructose 1,6-bisphosphate (FBP) in gluconeogenesis and the reverse reaction in glycolysis [H]

COG id: COG0191

COG function: function code G; Fructose/tagatose bisphosphate aldolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class II fructose-bisphosphate aldolase family [H]

Homologues:

Organism=Escherichia coli, GI87082041, Length=299, Percent_Identity=38.4615384615385, Blast_Score=199, Evalue=3e-52,
Organism=Escherichia coli, GI1789526, Length=309, Percent_Identity=36.8932038834951, Blast_Score=197, Evalue=7e-52,
Organism=Escherichia coli, GI1788072, Length=252, Percent_Identity=34.9206349206349, Blast_Score=146, Evalue=2e-36,
Organism=Saccharomyces cerevisiae, GI6322790, Length=347, Percent_Identity=27.6657060518732, Blast_Score=74, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011289
- InterPro:   IPR000771 [H]

Pfam domain/function: PF01116 F_bP_aldolase [H]

EC number: =4.1.2.13 [H]

Molecular weight: Translated: 33060; Mature: 32929

Theoretical pI: Translated: 5.10; Mature: 5.10

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVTTKEMFKKAYEGKYAIGAFNINNMEILQGVVNSAKAANSAVILQVSAGALKYAGPK
CCEECHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCHH
YLKAMVDAAIADTGIDVALHLDHGASLDVVKLAVESGFTSVMFDGSHYDYEENVAKTKEV
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VEYAHSHGVVVEAELGVLAGVEDDVQSDVHIYTDPEQAVDFVNRTGVDSLAIAIGTSHGA
HHHHHHCCEEEEECCCEEECCCHHHCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCC
YKFAGEAQLRFDILEEIQSKLPGYPIVLHGASSVDPESVDTCNKYGGEIKGAKGVPADML
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHH
RKASSMAVCKINMDTDLRLAMTAGIRKVLSEDPKQFDPRKYLGEGRSRIEKIVEDKIKNV
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGSADSLK
HCCCCCCC
>Mature Secondary Structure 
ALVTTKEMFKKAYEGKYAIGAFNINNMEILQGVVNSAKAANSAVILQVSAGALKYAGPK
CEECHHHHHHHHHCCCEEEEEEECCHHHHHHHHHHHHHCCCCEEEEEEECCCHHHCCHH
YLKAMVDAAIADTGIDVALHLDHGASLDVVKLAVESGFTSVMFDGSHYDYEENVAKTKEV
HHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
VEYAHSHGVVVEAELGVLAGVEDDVQSDVHIYTDPEQAVDFVNRTGVDSLAIAIGTSHGA
HHHHHHCCEEEEECCCEEECCCHHHCCCEEEEECHHHHHHHHHHCCCCCEEEEEECCCCC
YKFAGEAQLRFDILEEIQSKLPGYPIVLHGASSVDPESVDTCNKYGGEIKGAKGVPADML
EEECCCCHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHCCCCCCCCCCCCHHHH
RKASSMAVCKINMDTDLRLAMTAGIRKVLSEDPKQFDPRKYLGEGRSRIEKIVEDKIKNV
HHCCCEEEEEEECCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
LGSADSLK
HCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]