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The map label for this gene is sbcD [H]

Identifier: 153937118

GI number: 153937118

Start: 551812

End: 553041

Strand: Direct

Name: sbcD [H]

Synonym: CLC_0546

Alternate gene names: 153937118

Gene position: 551812-553041 (Clockwise)

Preceding gene: 153936456

Following gene: 153934920

Centisome position: 14.67

GC content: 31.14

Gene sequence:

>1230_bases
ATGAGAATATTGCATACCTCCGATTGGCACTTGGGAAAGAACCTTGAAGGCTTTAGTAGAATGGATGAACAGGAAAGATT
TATAGAGGATTTTATTAACATTGTGGATAAAAATAATATAGATATGGTGATAATAGCTGGAGATATATATGATAACGGGA
ATCCACCGGCTAGAGCAGAAAAAATGTTTTATGATTGTATAAAAAAAATAAGCAATAAGGGAGAAAGATTAGTTTTAATT
ATAGCAGGTAATCACGATAATCCAGAAAGATTAGTTGCTGCAAGTTCTTTAGCCTATGATGAGGGAATAATACTTATGGG
GACCCCTAAGACTATAGTTCCAAAGGGAAAGTATGGGAATTTTAAGGTAATAAATTCAGATTTAGCCTGTTTAGAAATAG
GGGTAAAGGAAGAAAAGGCAGTGATTATATCTTTACCTTATCCTAGTGAGAAAAGATTAAATGAAATACTGTCTAAAGAA
ATGGATAATGATGAGGATAGAAGAAAAACTTATTCAGAAAGGATAGGAGATATATTTTCTAATTTACAGGAAAAATATTA
TAAAGATAACACTGTTAATTTAGCTATTTCTCATATATTTGTAAATGGATCAGAGGAAACGGATTCTGAAAGGCCAATAC
AACTTGGAGGGAGTCTATCAGTAGATGTAGACAAGTTACCTAAAAAAGCACAATATATAGGCCTTGGGCATCTTCATAAA
CCACAAAAGGTAAGGGGGATAGATAATGCCTATTATTCTGGTTCACCACTGCCTTATAGTAAAAGCGAAAGAGGATATAG
TAAAGGATGCAAGATAATAGATGTACATCCAGGGGAGAAAGCTGTTATTGAAGAGGTTTATTTTAATAACTATAAACCTA
TAGAGGTGTGGCATTGCAACGGTATAAAAGAGGCTATAAATAAATGTGAAGAAAATAAAGAAAAGGATATGTGGCTATAT
TTACATATAAAAACAAAGGAATATATAAGTACAGAAGATATAAAAGCCTTAAAAGAAATAAAAAAAGATATAGTAGAGAT
AGTACCAGAAATAGAGGAAAAAAGAGAAGAAACTTTTAATGGAAATATAAAAGAAAAATCTATGGCAGAATTATTTAAAT
CCTTTTATTTAAATCAAAGAGGAATAGAGGCTAGTGAAGAATTAATGGATTTATTTTTAAGTATAGCTCAAGAGGAGGAT
GAATATGAAACCGAAGAAACTTGTGATTAA

Upstream 100 bases:

>100_bases
GATAATAATATAATTTATTTTTATATAGATTGTGAGTAGAATAAGCAAAATAATTTAATAAGATGGAAAGTTAACTAGAT
ACAAAAGGGAGATGAAGATC

Downstream 100 bases:

>100_bases
AGGACTAAACAGTTTTATAGAAAAACAGGAAATAGACTTTGAAACTTTAGTCAATAGAGGTTTGTTTGGCATATTTGGCC
CTACAGGCAGTGGAAAATCC

Product: putative nuclease SbcCD, D subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 409; Mature: 409

Protein sequence:

>409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKISNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIGVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLSVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIDVHPGEKAVIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD

Sequences:

>Translated_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKISNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIGVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLSVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIDVHPGEKAVIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD
>Mature_409_residues
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAEKMFYDCIKKISNKGERLVLI
IAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGNFKVINSDLACLEIGVKEEKAVIISLPYPSEKRLNEILSKE
MDNDEDRRKTYSERIGDIFSNLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLSVDVDKLPKKAQYIGLGHLHK
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIDVHPGEKAVIEEVYFNNYKPIEVWHCNGIKEAINKCEENKEKDMWLY
LHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFNGNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEED
EYETEETCD

Specific function: SbcCD cleaves DNA hairpin structures. These structures can inhibit DNA replication and are intermediates in certain DNA recombination reactions. The complex acts as a 3'->5' double strand exonuclease that can open hairpins. It also has a 5' single-strand

COG id: COG0420

COG function: function code L; DNA repair exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sbcD family [H]

Homologues:

Organism=Escherichia coli, GI1786598, Length=393, Percent_Identity=26.7175572519084, Blast_Score=134, Evalue=1e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004843
- InterPro:   IPR004593 [H]

Pfam domain/function: PF00149 Metallophos [H]

EC number: NA

Molecular weight: Translated: 46943; Mature: 46943

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHH
KMFYDCIKKISNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIGVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLSVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEECHHHCCCHHHEECCCCCCC
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIDVHPGEKAVIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEYETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHCCC
>Mature Secondary Structure
MRILHTSDWHLGKNLEGFSRMDEQERFIEDFINIVDKNNIDMVIIAGDIYDNGNPPARAE
CEEEECCCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHH
KMFYDCIKKISNKGERLVLIIAGNHDNPERLVAASSLAYDEGIILMGTPKTIVPKGKYGN
HHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHCCCCCEEEEECCCEECCCCCCCC
FKVINSDLACLEIGVKEEKAVIISLPYPSEKRLNEILSKEMDNDEDRRKTYSERIGDIFS
EEEECCCCEEEEECCCCCCEEEEECCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHH
NLQEKYYKDNTVNLAISHIFVNGSEETDSERPIQLGGSLSVDVDKLPKKAQYIGLGHLHK
HHHHHHHCCCCEEEEEEEEEEECCCCCCCCCCEEECCCEEECHHHCCCHHHEECCCCCCC
PQKVRGIDNAYYSGSPLPYSKSERGYSKGCKIIDVHPGEKAVIEEVYFNNYKPIEVWHCN
CHHHCCCCCCCCCCCCCCCCCCCCCHHHCCEEEEECCCCHHHHHHHHHCCCCCEEEEECC
GIKEAINKCEENKEKDMWLYLHIKTKEYISTEDIKALKEIKKDIVEIVPEIEEKREETFN
CHHHHHHHHHCCCCCCEEEEEEEECHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
GNIKEKSMAELFKSFYLNQRGIEASEELMDLFLSIAQEEDEYETEETCD
CCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9353931; 9384377; 10568751; 1646786; 8493111 [H]