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The map label for this gene is pyrG [H]

Identifier: 153937013

GI number: 153937013

Start: 162291

End: 163892

Strand: Direct

Name: pyrG [H]

Synonym: CLC_0180

Alternate gene names: 153937013

Gene position: 162291-163892 (Clockwise)

Preceding gene: 153936916

Following gene: 153934496

Centisome position: 4.32

GC content: 31.52

Gene sequence:

>1602_bases
ATGAGCACTAAATATATATTTGTAACAGGTGGTGTTGTTTCTTCATTAGGGAAAGGAATAACAGCAGCTTCTTTAGGAAG
ACTTTTAAAAAATAGAGGACTTAAAGTTTCAATACAAAAATTTGATCCATATATAAATATAGATCCAGGAACAATGAGTC
CTTATCAACATGGTGAAGTATTTGTAACAGAGGATGGTGCAGAAACAGATTTAGATTTAGGCCATTATGAAAGGTTTATA
GATGAAAATTTAAGTCAAAATAATAATGTAACTACAGGAAAGATTTACTGGTCTGTTATATCTAAAGAAAGAAAAGGTGA
TTATTTAGGTGCTACAGTGCAGGTAATACCTCATATAACTACAGAAATAAAATCTAGAGTATATAGAGTCGCTAAAGAAA
AAGAAGTAGATGTAGTTATAACTGAAATAGGTGGAACTATAGGAGATATAGAATCTCTACCTTTTTTAGAATCAATAAGG
CAAATCAAATATGAGGTTGGAAGAGAAAATGTATGTTTTATTCATGTAACCTTACTACCCTTTCTAGGAAAAGCAGGTGA
ATTAAAGACAAAGCCTACACAACACTCAGTTAAAGAACTTAGAGGAATAGGGATACAGCCAGATATCATAGTTTGTCGAT
CAGAAAAACAGATACCAGAGGAATTAAAGGGCAAAATAGGATTATTTTGTAATGTAGATAAAGAAGATGTCATACAAAAT
TTAGACGCAGAAAATCTTTATGAAGTACCATTAATGCTACATAAGGAAAATTTAGATACTTTAGTATGTAAGAAATTAGG
CCTAAATTATAGTAAAATAGATAACACGGAATGGATATCAATGGTAAATAATGCAAAAAACCTTTCTAAGAATACTACCA
TAGCTTTGGTAGGGAAGTATGTAGAATTACATGATGCTTATATATCAATAGTGGAAGCTTTAAACCATGGTGGTTATGCT
AATGATACTAAAGTAAATATAAAATGGATAAATTCTGAAGAGGTAAATGAAAATAACTTTGAACAATATTTAAAAGATGT
GGATGGTATATTGGTACCAGGTGGATTTGGTGATAGAGGAATAGAAGGTAAAATATGGGCTGCTAAATGGGCAAGAGAAA
ATAAGATACCTTTTTTAGGGATATGTCTTGGAATGCAATGTGCAGTAATAGAGTTTGCCAGAAATGTATTAAATTATAAA
GGAGCTCATAGTGCAGAGATAGATCCAAATACAAAATATCCAGTAATAGATTTAATGCCAGAGCAGAAGGATATAGATAA
TATGGGTGGAACTATGAGATTGGGAGCTTATCCTTGTAAATTACTTAAGGGTACAAATTCAGAAAAAGCTTATGGTCAGG
AGATTGTATATGAAAGACATAGGCATAGATATGAATTTAATAATGAATATAGGAGTAAACTAACAGAAGCGGGTTTAATA
TTGTCAGGAACAAGTCCAGACGATAGGTTAGTGGAGATAGTAGAGATAAATAATCATCCTTGGTATGTGGCTGTTCAATT
TCACCCAGAATTAAAATCAAGACCCAATAAGCCACATCCATTATTTAAAGACTTTGTAAAGGCCACTTTGGATAAAAAAT
AA

Upstream 100 bases:

>100_bases
TAATGATGTCAATAGATTTTATGTAAATTTAGTGCACAATAATACAAACTTAAACTTTGGAGGTGAAAACTGCTTTATTA
TATATTAAAGCAGGGGAGAT

Downstream 100 bases:

>100_bases
ATTTTATATACTAGTTAAAATTTAATATATGTTAATAAAAAACACCATCAGAATTTATAAAAATAAATAATTTTGGTGTT
GTTTTTTTATTTATCCGATG

Product: CTP synthetase

Products: NA

Alternate protein names: CTP synthetase; UTP--ammonia ligase [H]

Number of amino acids: Translated: 533; Mature: 532

Protein sequence:

>533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFI
DENLSQNNNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEVNENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK

Sequences:

>Translated_533_residues
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFI
DENLSQNNNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIR
QIKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYA
NDTKVNIKWINSEEVNENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYK
GAHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
>Mature_532_residues
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEVFVTEDGAETDLDLGHYERFID
ENLSQNNNVTTGKIYWSVISKERKGDYLGATVQVIPHITTEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQ
IKYEVGRENVCFIHVTLLPFLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQNL
DAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKYVELHDAYISIVEALNHGGYAN
DTKVNIKWINSEEVNENNFEQYLKDVDGILVPGGFGDRGIEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKG
AHSAEIDPNTKYPVIDLMPEQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLIL
SGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK

Specific function: Catalyzes the ATP-dependent amination of UTP to CTP with either L-glutamine or ammonia as the source of nitrogen [H]

COG id: COG0504

COG function: function code F; CTP synthase (UTP-ammonia lyase)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-1 domain [H]

Homologues:

Organism=Homo sapiens, GI148491070, Length=551, Percent_Identity=48.4573502722323, Blast_Score=507, Evalue=1e-143,
Organism=Homo sapiens, GI28559085, Length=548, Percent_Identity=49.0875912408759, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI28559083, Length=548, Percent_Identity=49.0875912408759, Blast_Score=503, Evalue=1e-142,
Organism=Homo sapiens, GI221316689, Length=548, Percent_Identity=49.0875912408759, Blast_Score=503, Evalue=1e-142,
Organism=Escherichia coli, GI1789142, Length=539, Percent_Identity=56.2152133580705, Blast_Score=610, Evalue=1e-176,
Organism=Caenorhabditis elegans, GI25148299, Length=593, Percent_Identity=38.7858347386172, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6319432, Length=558, Percent_Identity=45.3405017921147, Blast_Score=476, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6322563, Length=560, Percent_Identity=45.5357142857143, Blast_Score=470, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24664469, Length=550, Percent_Identity=49.0909090909091, Blast_Score=511, Evalue=1e-145,
Organism=Drosophila melanogaster, GI21357815, Length=495, Percent_Identity=47.2727272727273, Blast_Score=436, Evalue=1e-122,

Paralogues:

None

Copy number: 480 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004468
- InterPro:   IPR017456
- InterPro:   IPR017926
- InterPro:   IPR000991 [H]

Pfam domain/function: PF06418 CTP_synth_N; PF00117 GATase [H]

EC number: =6.3.4.2 [H]

Molecular weight: Translated: 60047; Mature: 59916

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: PS00442 GATASE_TYPE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIDENLSQNNNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCHHHCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEVNENNFEQYLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEIDPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC
>Mature Secondary Structure 
STKYIFVTGGVVSSLGKGITAASLGRLLKNRGLKVSIQKFDPYINIDPGTMSPYQHGEV
CCEEEEEECHHHHHHCCCCHHHHHHHHHHCCCCEEEEEECCCEEEECCCCCCCCCCCEE
FVTEDGAETDLDLGHYERFIDENLSQNNNVTTGKIYWSVISKERKGDYLGATVQVIPHIT
EEECCCCCCCCCHHHHHHHHHHCCCCCCCEEEHEEEEEEHHHHCCCCCCCCHHHHHHHHH
TEIKSRVYRVAKEKEVDVVITEIGGTIGDIESLPFLESIRQIKYEVGRENVCFIHVTLLP
HHHHHHHHHHHCCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEEEHHH
FLGKAGELKTKPTQHSVKELRGIGIQPDIIVCRSEKQIPEELKGKIGLFCNVDKEDVIQN
HCCCCCCCCCCCCHHHHHHHHCCCCCCCEEEECCCCCCCHHHCCCEEEEEECCHHHHHHC
LDAENLYEVPLMLHKENLDTLVCKKLGLNYSKIDNTEWISMVNNAKNLSKNTTIALVGKY
CCCCCHHHCCEEEECCCCHHHHHHHHCCCHHHCCCHHHHHHHHHHHHCCCCCEEEEEEHH
VELHDAYISIVEALNHGGYANDTKVNIKWINSEEVNENNFEQYLKDVDGILVPGGFGDRG
HHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEECCCCCCCC
IEGKIWAAKWARENKIPFLGICLGMQCAVIEFARNVLNYKGAHSAEIDPNTKYPVIDLMP
CCCCEEEEECCCCCCCCEEEEHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEECCC
EQKDIDNMGGTMRLGAYPCKLLKGTNSEKAYGQEIVYERHRHRYEFNNEYRSKLTEAGLI
CCCCHHHCCCEEEECCCCHHHHCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCEE
LSGTSPDDRLVEIVEINNHPWYVAVQFHPELKSRPNKPHPLFKDFVKATLDKK
EECCCCCCCEEEEEEECCCCEEEEEEECCHHHCCCCCCCHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA