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The map label for this gene is cca [H]

Identifier: 153936961

GI number: 153936961

Start: 2358839

End: 2360191

Strand: Reverse

Name: cca [H]

Synonym: CLC_2230

Alternate gene names: 153936961

Gene position: 2360191-2358839 (Counterclockwise)

Preceding gene: 153935804

Following gene: 153937696

Centisome position: 62.76

GC content: 25.5

Gene sequence:

>1353_bases
ATGATAAATAAAAATATAAAAATTCAAATATCTAAAGGGGTAGAGTATATTATAAATACTTTACAAGAAAATGGTTATGA
AGCATATATAGTAGGTGGTGCAGTTAGGGATAGCTTACTTGAAAGGAAAGTTAATGATTGGGATATAACTACTAGTGCCA
ATCCGCAAGAAGTAGTGAACATATTTGAAAATTTAGGTTATAAAATTATACCTACAGGATTAAAACACGGAACAGTAACA
ATATTAATCAATTCTATAGGTTATGAAGTAACTACATTTAGAGTTGATGGGGAATATGAAGATAATAGACATCCTAAAGA
AGTTAAGTTTACATCAAATTTAAGAGAAGATTTAAAGCGTAGAGATTTAACTATTAATGCAATGGCTTACAATGACAAAA
TAGGATTGGTAGATTATTTTTATGGATTGGAAGATTTAAATAATAAAATAATTAGATGCGTAGGAAATTCAAAAGATAGG
TTTAATGAAGATTCATTAAGAATGTTAAGATGTATAAGATTTGCTTCACAATTAAATTTTTGTATGGAAGAATCTATAAA
ACTTAACATAAGAAAATTAAGTAGAAATATAGCTAATGTATCTATGGAAAGAATCAGAGATGAATTATGCAAAATATTAG
TAAGCAGCCAACCGGTTTATGGCATAAGAAATATAGTTGAATTAAATTTAATGGATTATATTATTCCTGAACTAAAAGCT
TGTGTAGGCTTTGAGCAACATAATATACATCATGATAAAGATGTATATGGTCATATATTATCTGTAGTAGAAAATGTACC
AAATAAATTAGAATTAAGATTAGCAGCACTATTACATGACATAGGAAAACCTAGGTGTTTTAGCATAGGGGACAATGGAC
AAGGACATTTTTATGGTCATCAAAAGATAAGTGCAGATATGACTAAAGATATACTAAAAAGATTAAAATTTGATAATAAA
ACAATAGATAAGGTAGATAAATTGGTTTATAATCATATGACTAGATACAATAAATTAAGAACTCCTAACATAAAAAAGTT
TATTAATAAAGTAGGAATTGATAATTTAGATGATTTATTTGAGCTACAAATAGCTGATATAAAAGGAAGTGCTAAGGAAT
ATCATAGTTTCGATAATGTATTAAATCTAAAAATAAAGTGTGAAAAAATACTTAGTGAAAAACAACCTTTAACAATTAAG
GATTTAGATATTAATGGATATGACTTAATGAAGTTAGGAATAAACCAAGGAAAAGAAATAGGTATTATGTTAAATAAATT
ATTGGACATAATTTTAGAAAATCCTAATTTAAATAATAAAGAAGACTTAATTAAAATAGTTGAAAGTATTTAA

Upstream 100 bases:

>100_bases
GATTTAGATATAAGAGGCATAGCTACAGATTTACCTAACATGCCTAATTATAAAAAAGTAGAAGAGCTAGTTATAGAATT
TAATAAAGGTGTGATAAATA

Downstream 100 bases:

>100_bases
AAAAGATACTTATTTTAATATTCTAATAGCGTAATGTGAAATTTATGCGACAGAAGAGTAGGTGATAATTTGATTCAAAT
ATTAGAATTGTTTGGAGGAA

Product: polyA polymerase family protein

Products: NA

Alternate protein names: tRNA CCA-pyrophosphorylase; tRNA adenylyl-/cytidylyl- transferase; tRNA nucleotidyltransferase; tRNA-NT [H]

Number of amino acids: Translated: 450; Mature: 450

Protein sequence:

>450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFYGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFCMEESIKLNIRKLSRNIANVSMERIRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Sequences:

>Translated_450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFYGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFCMEESIKLNIRKLSRNIANVSMERIRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI
>Mature_450_residues
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVNIFENLGYKIIPTGLKHGTVT
ILINSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKRRDLTINAMAYNDKIGLVDYFYGLEDLNNKIIRCVGNSKDR
FNEDSLRMLRCIRFASQLNFCMEESIKLNIRKLSRNIANVSMERIRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGHQKISADMTKDILKRLKFDNK
TIDKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLFELQIADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIK
DLDINGYDLMKLGINQGKEIGIMLNKLLDIILENPNLNNKEDLIKIVESI

Specific function: Catalyzes the addition and repair of the essential 3'- terminal CCA sequence in tRNAs without using a nucleic acid template. Adds these three nucleotides in the order of C, C, and A to the tRNA nucleotide-73, using CTP and ATP as substrates and producing

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family. Bacterial CCA-adding enzyme type 3 subfamily [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=233, Percent_Identity=37.7682403433476, Blast_Score=134, Evalue=2e-31,
Organism=Escherichia coli, GI87081691, Length=242, Percent_Identity=29.7520661157025, Blast_Score=114, Evalue=1e-26,
Organism=Escherichia coli, GI1789436, Length=319, Percent_Identity=27.5862068965517, Blast_Score=100, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI71995920, Length=207, Percent_Identity=34.7826086956522, Blast_Score=110, Evalue=2e-24,
Organism=Saccharomyces cerevisiae, GI6321016, Length=298, Percent_Identity=29.1946308724832, Blast_Score=86, Evalue=1e-17,
Organism=Drosophila melanogaster, GI281360112, Length=286, Percent_Identity=33.5664335664336, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI21357337, Length=286, Percent_Identity=33.5664335664336, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023068
- InterPro:   IPR002646 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.21; =2.7.7.25 [H]

Molecular weight: Translated: 51805; Mature: 51805

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILINSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFYGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHCHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
CMEESIKLNIRKLSRNIANVSMERIRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIDKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHH
ELQIADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIKDLDINGYDLMKLGINQGKEI
HEEEECCCCCHHHHHHHHHHEEEEEEEHHHHCCCCCCEEEECCCCCEEEEEECCCCCCHH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC
>Mature Secondary Structure
MINKNIKIQISKGVEYIINTLQENGYEAYIVGGAVRDSLLERKVNDWDITTSANPQEVVN
CCCCCEEEEECCCHHHHHHHHHCCCCEEEEECHHHHHHHHHCCCCCCEECCCCCHHHHHH
IFENLGYKIIPTGLKHGTVTILINSIGYEVTTFRVDGEYEDNRHPKEVKFTSNLREDLKR
HHHHCCCEEEECCCCCCEEEEEEECCCCEEEEEEECCCCCCCCCCCCEEHHHHHHHHHHH
RDLTINAMAYNDKIGLVDYFYGLEDLNNKIIRCVGNSKDRFNEDSLRMLRCIRFASQLNF
CCCEEEEEEECCCCCHHHHHHCHHHHCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHH
CMEESIKLNIRKLSRNIANVSMERIRDELCKILVSSQPVYGIRNIVELNLMDYIIPELKA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHH
CVGFEQHNIHHDKDVYGHILSVVENVPNKLELRLAALLHDIGKPRCFSIGDNGQGHFYGH
HHCCHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCEEEC
QKISADMTKDILKRLKFDNKTIDKVDKLVYNHMTRYNKLRTPNIKKFINKVGIDNLDDLF
CHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCCHHH
ELQIADIKGSAKEYHSFDNVLNLKIKCEKILSEKQPLTIKDLDINGYDLMKLGINQGKEI
HEEEECCCCCHHHHHHHHHHEEEEEEEHHHHCCCCCCEEEECCCCCEEEEEECCCCCCHH
GIMLNKLLDIILENPNLNNKEDLIKIVESI
HHHHHHHHHHHCCCCCCCCHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA