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The map label for this gene is hsdM [H]

Identifier: 153936955

GI number: 153936955

Start: 750601

End: 752058

Strand: Direct

Name: hsdM [H]

Synonym: CLC_0732

Alternate gene names: 153936955

Gene position: 750601-752058 (Clockwise)

Preceding gene: 153937827

Following gene: 153934972

Centisome position: 19.96

GC content: 31.41

Gene sequence:

>1458_bases
ATGATTACAGGTGAGTTAAAAAGTAAGATAGATAGAATTTGGGAAACCTTTTGGACTGGTGGCATCACTAATCCATTAGA
GGTTATAGAACAATTTACATATTTTTTATTTATAAAAGATTTAGATGATAACGAAATATTAGCTGAAAGTGATGCTGAAT
TACTTGGCATACCCTTTGAGGGAATGTTTCCAAGTGATAAGCAGTATTTAAGATGGAGTAAATTTAAAAATGAACCAGCA
GGGGAAATGTATAGGATAGTATCAGAAGAAGTATTTCCCTTTATAAAAGATATTCATGGAGATGAGCAATCTGCATACTC
AAAATATATGTCAGATGCTATGTTTAAGATACCAACTCCACTTATGTTATCTAAGATAGTTGATTCAATAGATAATATTA
ATATGAATGATAAAGATACTAAGGGAGATTTATATGAATATCTTTTATCTAAAATAGCTCAAGCAGGTACAAATGGTCAG
TTTAGAACTCCAAGACACATAATAAAAATGATGGCAGAATTAATGAAGCCAACACCAGAGGATATTATAGTAGATCCAGC
TATGGGTACAGCAGGTTTTCTAGTTGGAGCAGAGGAATATTTAAGAGAAAATCACAATGATCTATTTTTTGTTCAAGGAT
TAAAAGAACATTTTAATAATAAGATGTTTAATGGATTTGATATGGACAGAACTATGCTTAGAATTGGTGCTATGAACATG
ATGCTTCATGGAGTGGACAATCCTAATATTGAGTATAAAGATTCACTTTCGGAAACTAATAAGGACAGTGAAAAATATAC
TCTAGTACTTGCAAATCCCCCATTTAAAGGGAGCCTAGACTACGAAGCGGTTTCAGCAGATCTTTTAAAAGTTAGTAAGA
CAAAGAAAACAGAATTATTGTTCTTAGCATTATTTTTAAGAATATTAAAAACTGGTGGAAGATGTGCATCCATTGTTCCA
GATGGAGTATTATTTGGTTCAACTAAAGGACATAAGGATATAAGACGTGAAATAGTTGATAACAATAAGCTCGAAGCTAT
AATAAGTATGCCAAGTGGAGTGTTTAAGCCATACGCAGGAGTATCTACAGCTATTATGATATTCACTAAAACTGGTACTG
GTGGAACAGATAAAGTTTGGTTCTATGATATGAAAGCAGATGGATATTCCCTAGATGATAAAAGAAATCCTATAGAAGAT
AACGATATGCCGGATATAATAGAAAGATTCAGTAATTTAGACAAAGAAGAAGATAGAAATAGAACAGAACAATCCTTCTT
TGTACCAGTAGAAGAAATAAGAGAAAATGATTATGACTTATCCATTAATAAATACAAAGAAATAGAATATGAAGAAGTAG
TTTATGATGAACCTAAGGTTATTTTAGAGAGAGTTAAGAAACTAGAAAAAGAAATAACTGAGGGGATAGATGAATTAGAA
AAAATGATAGAGGGGTAA

Upstream 100 bases:

>100_bases
AAACTAATGCAATTATTTTTTACATATAAATAAGAAGAAATTATTAAAATAAAAAACTGAATTATATTATAACAATATAA
CTAATGATGGAGGAAACATA

Downstream 100 bases:

>100_bases
GTGAGGCTCCAAATCTTGTATTTGGTTAGTCGAACTTACTCAGCGAACGTATGTGAGTCGAGTTTCATTTAAAAAGGAAA
ATGAGGTATATAATATGTTA

Product: putative type I restriction-modification system, M subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEPA
GEMYRIVSEEVFPFIKDIHGDEQSAYSKYMSDAMFKIPTPLMLSKIVDSIDNINMNDKDTKGDLYEYLLSKIAQAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLRENHNDLFFVQGLKEHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADLLKVSKTKKTELLFLALFLRILKTGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDMPDIIERFSNLDKEEDRNRTEQSFFVPVEEIRENDYDLSINKYKEIEYEEVVYDEPKVILERVKKLEKEITEGIDELE
KMIEG

Sequences:

>Translated_485_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEPA
GEMYRIVSEEVFPFIKDIHGDEQSAYSKYMSDAMFKIPTPLMLSKIVDSIDNINMNDKDTKGDLYEYLLSKIAQAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLRENHNDLFFVQGLKEHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADLLKVSKTKKTELLFLALFLRILKTGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDMPDIIERFSNLDKEEDRNRTEQSFFVPVEEIRENDYDLSINKYKEIEYEEVVYDEPKVILERVKKLEKEITEGIDELE
KMIEG
>Mature_485_residues
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFEGMFPSDKQYLRWSKFKNEPA
GEMYRIVSEEVFPFIKDIHGDEQSAYSKYMSDAMFKIPTPLMLSKIVDSIDNINMNDKDTKGDLYEYLLSKIAQAGTNGQ
FRTPRHIIKMMAELMKPTPEDIIVDPAMGTAGFLVGAEEYLRENHNDLFFVQGLKEHFNNKMFNGFDMDRTMLRIGAMNM
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADLLKVSKTKKTELLFLALFLRILKTGGRCASIVP
DGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAGVSTAIMIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIED
NDMPDIIERFSNLDKEEDRNRTEQSFFVPVEEIRENDYDLSINKYKEIEYEEVVYDEPKVILERVKKLEKEITEGIDELE
KMIEG

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=485, Percent_Identity=27.4226804123711, Blast_Score=157, Evalue=1e-39,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 55643; Mature: 55643

Theoretical pI: Translated: 4.49; Mature: 4.49

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEECCCCCEEECCCC
GMFPSDKQYLRWSKFKNEPAGEMYRIVSEEVFPFIKDIHGDEQSAYSKYMSDAMFKIPTP
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LMLSKIVDSIDNINMNDKDTKGDLYEYLLSKIAQAGTNGQFRTPRHIIKMMAELMKPTPE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DIIVDPAMGTAGFLVGAEEYLRENHNDLFFVQGLKEHFNNKMFNGFDMDRTMLRIGAMNM
CEEECCCCCCHHHHCCCHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADLLKVSKTKKTELL
HHCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHH
FLALFLRILKTGGRCASIVPDGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAG
HHHHHHHHHHCCCCEEECCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
VSTAIMIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIEDNDMPDIIERFSNLDKEEDRN
CCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHC
RTEQSFFVPVEEIRENDYDLSINKYKEIEYEEVVYDEPKVILERVKKLEKEITEGIDELE
CCHHHHCCCHHHHCCCCCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KMIEG
HHHCC
>Mature Secondary Structure
MITGELKSKIDRIWETFWTGGITNPLEVIEQFTYFLFIKDLDDNEILAESDAELLGIPFE
CCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHEEEEEECCCCCEEECCCCCEEECCCC
GMFPSDKQYLRWSKFKNEPAGEMYRIVSEEVFPFIKDIHGDEQSAYSKYMSDAMFKIPTP
CCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCH
LMLSKIVDSIDNINMNDKDTKGDLYEYLLSKIAQAGTNGQFRTPRHIIKMMAELMKPTPE
HHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHCCCCC
DIIVDPAMGTAGFLVGAEEYLRENHNDLFFVQGLKEHFNNKMFNGFDMDRTMLRIGAMNM
CEEECCCCCCHHHHCCCHHHHHCCCCCEEEEEHHHHHCCCCCCCCCCHHHHHHHHHHHHH
MLHGVDNPNIEYKDSLSETNKDSEKYTLVLANPPFKGSLDYEAVSADLLKVSKTKKTELL
HHCCCCCCCCCCHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHHHHCCHHHHHH
FLALFLRILKTGGRCASIVPDGVLFGSTKGHKDIRREIVDNNKLEAIISMPSGVFKPYAG
HHHHHHHHHHCCCCEEECCCCCEEECCCCCHHHHHHHHHCCCCEEEEEECCCCCCCCCCC
VSTAIMIFTKTGTGGTDKVWFYDMKADGYSLDDKRNPIEDNDMPDIIERFSNLDKEEDRN
CCEEEEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCHHHHC
RTEQSFFVPVEEIRENDYDLSINKYKEIEYEEVVYDEPKVILERVKKLEKEITEGIDELE
CCHHHHCCCHHHHCCCCCCCCCHHHHCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH
KMIEG
HHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]