Click here to switch to the map view.

The map label for this gene is eda [H]

Identifier: 153936859

GI number: 153936859

Start: 336150

End: 336797

Strand: Direct

Name: eda [H]

Synonym: CLC_0351

Alternate gene names: 153936859

Gene position: 336150-336797 (Clockwise)

Preceding gene: 153936055

Following gene: 153937236

Centisome position: 8.94

GC content: 30.56

Gene sequence:

>648_bases
GTGATTAAGATGAAGGTTTTAAATGCTCTAAAAAAATGTAAGATAGTAGCAGTTGTTCGTGGGGAAACAAAGGAAGTAGG
TATTAAAATATCAGAAAGCTGTATTAAAGGGAATGTTAAAGCTATAGAAGTTGCATACACAAATAAATTTGCTAATGATA
TAATAGAAGAACTTTCAGAAAAGTATTCAAAAGATGATGAGGTTGTTATAGGTGCTGGAACAGTTTTGGATTCAGAAACA
GCAAGAATGGCAATATTAAGAGGAGCAAAATATATAGTGTCTCCATCATTTAGTGAAGAAACAGCAAAGATTTGTAATAG
ATATAATATTCCATATATACCAGGAGTGATGACTATAACTGAAATAGTCACAGCTTATGAAAGTGGAGTAGATATTGTGA
AGGTATTTCCAGGAAGTGCATTTGGACAAGGTTATATAAAATCTATTAAAGGACCATTACCATATGCAAATATAATGGTA
ACAGGTGGAGTGAACTTAGATAATATAGATTCATGGGCAAATACATCTGTAGATTTAGTAGGTATTGGTGGAGAATTAAA
TAAACTTGGAGAGCTAGGAAAATTTGATGAAATAGAATTAATTTGTAATCAATATGTAGATAAATTTAATAAAGCAAGGG
GTATATAA

Upstream 100 bases:

>100_bases
ATTTTCAGAGAGAGCAGTTTCACCAATGAAAGGTGGATATATGGAATAATAATATTTCTGTAAGAGTTAGTACAATAATA
GATTTATGGAGGTTTTTATT

Downstream 100 bases:

>100_bases
GATGAAAGTAGTTGGTTTTGGAGAGATATTACTTAGATTATCTACTAAGAAAGGAATGCTTTTTTTAAATTCTAAGGAAT
TTGATTCTAATTATGGTGGT

Product: 2-dehydro-3-deoxyphosphogluconate aldolase/4-hydroxy-2-oxoglutarate aldolase

Products: NA

Alternate protein names: 4-hydroxy-2-oxoglutarate aldolase; 2-keto-4-hydroxyglutarate aldolase; KHG-aldolase; 2-dehydro-3-deoxy-phosphogluconate aldolase; 2-keto-3-deoxy-6-phosphogluconate aldolase; KDPG-aldolase; Phospho-2-dehydro-3-deoxygluconate aldolase; Phospho-2-keto-3-deoxygluconate aldolase [H]

Number of amino acids: Translated: 215; Mature: 215

Protein sequence:

>215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYSKDDEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKICNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTSVDLVGIGGELNKLGELGKFDEIELICNQYVDKFNKARGI

Sequences:

>Translated_215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYSKDDEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKICNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTSVDLVGIGGELNKLGELGKFDEIELICNQYVDKFNKARGI
>Mature_215_residues
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSEKYSKDDEVVIGAGTVLDSET
ARMAILRGAKYIVSPSFSEETAKICNRYNIPYIPGVMTITEIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMV
TGGVNLDNIDSWANTSVDLVGIGGELNKLGELGKFDEIELICNQYVDKFNKARGI

Specific function: KEY ENZYME IN THE ENTNER-DOUDOROFF PATHWAY. PARTICIPATES IN THE REGULATION OF THE INTRACELLULAR LEVEL OF GLYOXYLATE. [C]

COG id: COG0800

COG function: function code G; 2-keto-3-deoxy-6-phosphogluconate aldolase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the KHG/KDPG aldolase family [H]

Homologues:

Organism=Escherichia coli, GI48994954, Length=181, Percent_Identity=30.939226519337, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1788156, Length=187, Percent_Identity=28.3422459893048, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000887
- InterPro:   IPR013785 [H]

Pfam domain/function: PF01081 Aldolase [H]

EC number: =4.1.3.16; =4.1.2.14 [H]

Molecular weight: Translated: 23384; Mature: 23384

Theoretical pI: Translated: 5.45; Mature: 5.45

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSE
CCHHHHHHHHHHCEEEEEEECCCHHHCEEEEHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
KYSKDDEVVIGAGTVLDSETARMAILRGAKYIVSPSFSEETAKICNRYNIPYIPGVMTIT
HHCCCCCEEEECCCEECCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
EIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMVTGGVNLDNIDSWANTSVDLV
HHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEE
GIGGELNKLGELGKFDEIELICNQYVDKFNKARGI
ECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKMKVLNALKKCKIVAVVRGETKEVGIKISESCIKGNVKAIEVAYTNKFANDIIEELSE
CCHHHHHHHHHHCEEEEEEECCCHHHCEEEEHHHHCCCCEEEEEEEHHHHHHHHHHHHHH
KYSKDDEVVIGAGTVLDSETARMAILRGAKYIVSPSFSEETAKICNRYNIPYIPGVMTIT
HHCCCCCEEEECCCEECCHHHHHHHHHCCCEEECCCCCHHHHHHHHCCCCCCCCHHHHHH
EIVTAYESGVDIVKVFPGSAFGQGYIKSIKGPLPYANIMVTGGVNLDNIDSWANTSVDLV
HHHHHHHCCCCEEEECCCCCCCCHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCEEEE
GIGGELNKLGELGKFDEIELICNQYVDKFNKARGI
ECCCCHHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8760912; 9384377 [H]