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The map label for this gene is braE [H]

Identifier: 153936686

GI number: 153936686

Start: 537916

End: 538860

Strand: Direct

Name: braE [H]

Synonym: CLC_0529

Alternate gene names: 153936686

Gene position: 537916-538860 (Clockwise)

Preceding gene: 153936150

Following gene: 153934629

Centisome position: 14.3

GC content: 27.83

Gene sequence:

>945_bases
ATGAAGTTATTTAAAAATAACAAAATTATGTTTTTAATAATTTTGGCAATATTGCTTGTAGCACCTTTTATGATTACAAA
TCAATATATTCTACATATTATTATAACTATACTTTTATATAGCATATTAAGTATGAGTCTTAATTTTGTTAGCGGTAATG
CAGGGCAAATTTCTGTAGGACATGCTGCTTTTTATGGTATAGGGGCTTATACAACTAGTATACTTATGATTAGTGCTCAC
TTGCCTTTTATAACTGCTATGTTATTAGGGGGATTGTTAGCAGGCATATGTGGAATATTTTTAGGTATACCATCTTTACG
TGTAAAAGGGAACTATCTTTGCATAATAACTCTTGGATTTGGAGAAATAGTAAAATTAATATTGTTAAATTGGGATAAAG
TAACCGGTGGACCTATGGGTATCTCCTCTATACCTAGTCCTGAATTTTTAAGTATATTTAAGGATAAAAACTTAGGATTT
TATTATTTAATATTACTTTTATTTGTGGTTTCCTTTGTTATATTATATAAATTAACTTATTCTAATTATGGATTGCTTAT
GTTAGCTGTTAGAGAAAATGAAATAGCGGCTACATCATTAGGAATAAATATAACCAGCATAAAACTTTTTTCTTTTATAA
TAAGTTCGTTTATAGCAGGCATAGCTGGAGGATTTTATGCAACTTATGTTTCCTTTATAAGTCCAGAGTCCTTTATGTTT
AAAGAATCCATAACTATTTTGGCTATGGTTATAATAGGTGGAAAAGGAAATATAGTTGGATCAATTATTGGAGCTATAGT
TTTGGCAATTATTCCAGAGCTTTTAAGAGAATTTAATGATTATAGAATGTTATTTTATGGTTTAGGTTTAGTATTGATGA
TTGTATTTAGACCTGATGGAATTTACGGATTGAAATATAGAAAGAAATTTACATATAAATCATAG

Upstream 100 bases:

>100_bases
TACATCCTATGTAGATGGAATAGCCTTTATAATATTAGTTCTAGTATTAATTATTAAACCTAATGGAATATTTGGTGAAG
AAATTAAGGAGAAAGTATAG

Downstream 100 bases:

>100_bases
AAGGGGTATATATGAACATATTAGAAGTAAAAGATTTAAATATAAATTTTGGGGGAATAACTGCAATAGATAATCTGAAT
TTTTCAGTGAAAAAAGGAGA

Product: putative high-affinity branched-chain amino acid ABC transporter, permease protein

Products: ADP; phosphate; L-valine [Cytoplasm]; ADP; L-iso-leucine [Cytoplasm]; L-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 314; Mature: 314

Protein sequence:

>314_residues
MKLFKNNKIMFLIILAILLVAPFMITNQYILHIIITILLYSILSMSLNFVSGNAGQISVGHAAFYGIGAYTTSILMISAH
LPFITAMLLGGLLAGICGIFLGIPSLRVKGNYLCIITLGFGEIVKLILLNWDKVTGGPMGISSIPSPEFLSIFKDKNLGF
YYLILLLFVVSFVILYKLTYSNYGLLMLAVRENEIAATSLGINITSIKLFSFIISSFIAGIAGGFYATYVSFISPESFMF
KESITILAMVIIGGKGNIVGSIIGAIVLAIIPELLREFNDYRMLFYGLGLVLMIVFRPDGIYGLKYRKKFTYKS

Sequences:

>Translated_314_residues
MKLFKNNKIMFLIILAILLVAPFMITNQYILHIIITILLYSILSMSLNFVSGNAGQISVGHAAFYGIGAYTTSILMISAH
LPFITAMLLGGLLAGICGIFLGIPSLRVKGNYLCIITLGFGEIVKLILLNWDKVTGGPMGISSIPSPEFLSIFKDKNLGF
YYLILLLFVVSFVILYKLTYSNYGLLMLAVRENEIAATSLGINITSIKLFSFIISSFIAGIAGGFYATYVSFISPESFMF
KESITILAMVIIGGKGNIVGSIIGAIVLAIIPELLREFNDYRMLFYGLGLVLMIVFRPDGIYGLKYRKKFTYKS
>Mature_314_residues
MKLFKNNKIMFLIILAILLVAPFMITNQYILHIIITILLYSILSMSLNFVSGNAGQISVGHAAFYGIGAYTTSILMISAH
LPFITAMLLGGLLAGICGIFLGIPSLRVKGNYLCIITLGFGEIVKLILLNWDKVTGGPMGISSIPSPEFLSIFKDKNLGF
YYLILLLFVVSFVILYKLTYSNYGLLMLAVRENEIAATSLGINITSIKLFSFIISSFIAGIAGGFYATYVSFISPESFMF
KESITILAMVIIGGKGNIVGSIIGAIVLAIIPELLREFNDYRMLFYGLGLVLMIVFRPDGIYGLKYRKKFTYKS

Specific function: Component of the high affinity leucine, isoleucine, valine, transport system (LIV-I), which is operative without Na(+) and is specific for alanine and threonine, in addition to branched-chain amino acids [H]

COG id: COG4177

COG function: function code E; ABC-type branched-chain amino acid transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the binding-protein-dependent transport system permease family. LivHM subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789865, Length=303, Percent_Identity=39.2739273927393, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001851
- InterPro:   IPR021807 [H]

Pfam domain/function: PF02653 BPD_transp_2; PF11862 DUF3382 [H]

EC number: NA

Molecular weight: Translated: 34560; Mature: 34560

Theoretical pI: Translated: 9.80; Mature: 9.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFKNNKIMFLIILAILLVAPFMITNQYILHIIITILLYSILSMSLNFVSGNAGQISVG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEC
HAAFYGIGAYTTSILMISAHLPFITAMLLGGLLAGICGIFLGIPSLRVKGNYLCIITLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECH
GEIVKLILLNWDKVTGGPMGISSIPSPEFLSIFKDKNLGFYYLILLLFVVSFVILYKLTY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
SNYGLLMLAVRENEIAATSLGINITSIKLFSFIISSFIAGIAGGFYATYVSFISPESFMF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KESITILAMVIIGGKGNIVGSIIGAIVLAIIPELLREFNDYRMLFYGLGLVLMIVFRPDG
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
IYGLKYRKKFTYKS
EEECEEECCCCCCC
>Mature Secondary Structure
MKLFKNNKIMFLIILAILLVAPFMITNQYILHIIITILLYSILSMSLNFVSGNAGQISVG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCCCEEEEC
HAAFYGIGAYTTSILMISAHLPFITAMLLGGLLAGICGIFLGIPSLRVKGNYLCIITLGF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCEEEEEEECH
GEIVKLILLNWDKVTGGPMGISSIPSPEFLSIFKDKNLGFYYLILLLFVVSFVILYKLTY
HHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHC
SNYGLLMLAVRENEIAATSLGINITSIKLFSFIISSFIAGIAGGFYATYVSFISPESFMF
CCCCEEEEEEECCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
KESITILAMVIIGGKGNIVGSIIGAIVLAIIPELLREFNDYRMLFYGLGLVLMIVFRPDG
HHHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEECCCC
IYGLKYRKKFTYKS
EEECEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-valine [Periplasm]; H2O; ATP; L-iso-leucine [Periplasm]; L-leucine [Periplasm] [C]

Specific reaction: ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2120183; 10984043 [H]