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The map label for this gene is pflD [H]

Identifier: 153936671

GI number: 153936671

Start: 2184335

End: 2186875

Strand: Reverse

Name: pflD [H]

Synonym: CLC_2060

Alternate gene names: 153936671

Gene position: 2186875-2184335 (Counterclockwise)

Preceding gene: 153937642

Following gene: 153935989

Centisome position: 58.15

GC content: 34.99

Gene sequence:

>2541_bases
TTGGATATACGTGAATTTTCAAATAAATTTATGGAAGCTACTAAAAATATGTCTGATGAAGAACGCGCTGGATTAATGAA
GATGTTTCAAAGTGTTTCTAATGAAATAACAAAAGAAGAACCAGCTACATCAAAGGTTGCGTGTGACAATAATGGTGAAA
TACCAGATGGAATGACAGAACGTCTAGTTAAGTTAAAGGAAACTTATCTAAAACATGTTCCAACAATAACAACTCATAGA
GCAAGAGCTATTACTAAAATAGCAAAAGAAAACCCTGGTACTCCTAAATCAGTTTTACGTGGTAAATGTTTTAAACATTG
TTGCGAAACTGCACCACTTGTAATTCAAGACAATGAACTTATAGTTGGAGCACCAAATGGACAACCTCGTGCAGGAGCAT
TTTCTCCTGATATAGCTTGGAGATGGATGGTTGATGAAATTGATACAATAGGAACTCGTCCACAAGACCCATTCTACATC
TCAGAAGAAGATAAGAAAATCATGCGTGAGGAGTTATTCCCATATTGGGCGGGTAAATCCGTTGATGAATACTGCGAAGA
TCAATATCGTGAAGCAGGAGTATGGGAACTTTCAGGAGAATCTTTTGTTTCTGACTGTTCATACCACGCAGTAAATGGTG
GAGGAGACTCTAACCCAGGATATGACGTTGTATTAATGAAAAAAGGTATGCTTGACATAAAGAGAGAAGCAGAAGAAAAA
TTAGCTGAACTTAAATATGAAAATCCAGAAGATATAGACAAAATCTATTTCTACAAATCATTAATTGATACTGCTGAAGG
TGTTATGATATATGCTAAACGTATGTCAGATTATGCTGCTGAATTAGCTCAAAAAGAAACTAACCCTAAGCGTAAAGCAG
AACTTCAAAAGATTTCTGAAATAAATGCTAGAGTTCCAGCACATAAGCCAAGTACTTATTGGGAAGCAATTCAAGCAGTT
TGGACTATAGAATCATTACTTGTAGTTGAGGAAAATCAAACAGGTATGTCTATAGGACGTGTTGACCAATATATGTACCC
ATTCTATAAAGCTGATATTGAAGCTGGACGTATGACTGATTATGAAGCATTTGAATTATCAGGTTGTATGCTTATAAAAA
TGTCTGAAATGATGTGGATAACAAGTGAAGGTGGTTCTAAATTCTTCGCAGGTTATCAACCTTTTGTAAATATGTGTGTA
GGTGGTGTTACTAGAGAAGGCCGTGACGCTACAAACGAATTAACATACCTTTTAATGGATGCAGTTCGTCATGTTAAAAT
ATATCAACCATCTTTAGCTTGCCGTATACACAAATCATCACCACAAAAATATCTTAAAAAGATAGTTGACGTTATTCGTG
CAGGTATGGGATTCCCAGCATGCCACTTTGACGATGTTCATATAAAAATTATGTTAGCTAAAGGTGTTTCTATAGAAGAC
GCAAGAGATTACTGCTTAATGGGATGTGTTGAACCACAAAAATCAGGAAGACTATATCAATGGACTTCTACAGGATACAC
TCAATGGCCTATATGTATAGAACTTGTTTTAAACAATGGTGTACCATTATGGTATGGTAAACAAGTATGCCCAGATATGG
GAGACTTAAGCCAGTTTAAAACTTATGAACAATTTGAAGCAGCTGTTAAAGAACAAATTAAATTCATTACTAAATGGACA
AGTGTTGCTACAGTAATTTCTCAACGTGTTCATAAAGAACTTGCTCCAAAGCCACTTATGTCTATGATGTATGAAGGTTG
TATGGAAAATGGTAGAGGAGTAGAAGCCGGCGGAGCTATGTATAACTTCGGACCTGGAGTAGTATGGAGTGGTCTTGCTA
CTTATGCAGATTCTATGGCAGCTATAAAGAAATTAGTATTTGAAGATAAAAAATATACTCTACAAGAAATGAACGAAGCT
TTAAAAGCAGATTTCGTTGGATATGAACAATTAAGAAAAGATTGTTTAGAAGCACCTAAGTATGGTAACGATGATGATTA
TGCAGATTTAATTGCTGCTGATTTAATTAACTTTACAGAACAAGAACATCGTAAATATAAGACATTATATTCAGTACTTA
GTCATGGTACTTTATCAATATCAAACAATACTCCATTTGGACAAATGACTGGAGCTACAGCAAATGGACGTAGAGCATGG
ATGCCTTTATCAGATGGTATAAGTCCATCACAAGGCGCTGATTTTAAAGGCCCTACTTCTATAATAAAATCTGTTTCTAA
GATGTCTTGTGAAGATATGAATATAGGTATGGTTCATAACTTTAAGTTAATAGCTGGTCTTCTTGATACACCAGAAGGAG
AACAAGGAATCATTACATTATTACGTAGTGCTTGTGCCCTTCAACTTGGAGAAGTTCAATTTAACTATTTAGACAACAAG
ACTTTAATAGAAGCTCAAAAACATCCAGATCAATATCGTGATTTAATTGTTCGTGTTGCTGGATACAGTGCATTCTTCGT
TGAGTTATGTAAAGATGTTCAAGATGAAATTATAAGTAGAACTATGCTTACACATTTCTAA

Upstream 100 bases:

>100_bases
TATAAGTTATTAGAGAGTATTTTAAAAAAAGTTTTACAGGACTTAAAATAAAAAATCATATTTAATATATGAAATACATT
AAAGAATGTGAGGGAATAAA

Downstream 100 bases:

>100_bases
TAAAAATTTGATGGAAAATATATAACTTATAGAAAATATATAACTTAGTCCCTTTAAAATAATTTATATAAAGTCTGCAA
TCTACAACTTCTTTTGTAGA

Product: formate acetyltransferase

Products: NA

Alternate protein names: Pyruvate formate-lyase 2 [H]

Number of amino acids: Translated: 846; Mature: 846

Protein sequence:

>846_residues
MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITKEEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR
ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI
SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK
LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELQKISEINARVPAHKPSTYWEAIQAV
WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV
GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED
ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT
SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA
LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW
MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK
TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF

Sequences:

>Translated_846_residues
MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITKEEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR
ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI
SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK
LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELQKISEINARVPAHKPSTYWEAIQAV
WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV
GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED
ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT
SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA
LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW
MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK
TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF
>Mature_846_residues
MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITKEEPATSKVACDNNGEIPDGMTERLVKLKETYLKHVPTITTHR
ARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNELIVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYI
SEEDKKIMREELFPYWAGKSVDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK
LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELQKISEINARVPAHKPSTYWEAIQAV
WTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTDYEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCV
GGVTREGRDATNELTYLLMDAVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED
ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFKTYEQFEAAVKEQIKFITKWT
SVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAMYNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEA
LKADFVGYEQLRKDCLEAPKYGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW
MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITLLRSACALQLGEVQFNYLDNK
TLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISRTMLTHF

Specific function: Glucose metabolism (nonoxidative conversion). [C]

COG id: COG1882

COG function: function code C; Pyruvate-formate lyase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pyruvate formate lyase domain [H]

Homologues:

Organism=Escherichia coli, GI1790388, Length=796, Percent_Identity=33.6683417085427, Blast_Score=434, Evalue=1e-122,
Organism=Escherichia coli, GI1787044, Length=819, Percent_Identity=32.4786324786325, Blast_Score=404, Evalue=1e-113,
Organism=Escherichia coli, GI48994926, Length=591, Percent_Identity=22.673434856176, Blast_Score=125, Evalue=8e-30,
Organism=Escherichia coli, GI1787131, Length=543, Percent_Identity=22.2836095764273, Blast_Score=119, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001150
- InterPro:   IPR019777
- InterPro:   IPR004184
- InterPro:   IPR010098 [H]

Pfam domain/function: PF01228 Gly_radical; PF02901 PFL [H]

EC number: =2.3.1.54 [H]

Molecular weight: Translated: 95418; Mature: 95418

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00850 GLY_RADICAL_1 ; PS51149 GLY_RADICAL_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
6.7 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
6.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITKEEPATSKVACDNNGEIPDGMTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH
RLVKLKETYLKHVPTITTHRARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCEE
IVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYISEEDKKIMREELFPYWAGKS
EEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCCC
VDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK
HHHHHHHHHHHCCCEEECCCHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH
LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELQKISE
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
INARVPAHKPSTYWEAIQAVWTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTD
HHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCCCC
YEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCVGGVTREGRDATNELTYLLMD
CCHHCCCCEEEEEEECEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED
HHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC
ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFK
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCEEECHHHCCCCCCHHHHH
TYEQFEAAVKEQIKFITKWTSVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCE
YNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEALKADFVGYEQLRKDCLEAPK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
YGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHH
LRSACALQLGEVQFNYLDNKTLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISR
HHHHHHHHHCCEEEEECCCCCEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
TMLTHF
HHHHCC
>Mature Secondary Structure
MDIREFSNKFMEATKNMSDEERAGLMKMFQSVSNEITKEEPATSKVACDNNGEIPDGMTE
CCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEECCCCCCCCHHHH
RLVKLKETYLKHVPTITTHRARAITKIAKENPGTPKSVLRGKCFKHCCETAPLVIQDNEL
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCEEEECCEE
IVGAPNGQPRAGAFSPDIAWRWMVDEIDTIGTRPQDPFYISEEDKKIMREELFPYWAGKS
EEECCCCCCCCCCCCCCCEEEEEHHHHHHCCCCCCCCEEECCHHHHHHHHHHCCCCCCCC
VDEYCEDQYREAGVWELSGESFVSDCSYHAVNGGGDSNPGYDVVLMKKGMLDIKREAEEK
HHHHHHHHHHHCCCEEECCCHHHHCCCCEEECCCCCCCCCEEEEEEECCCHHHHHHHHHH
LAELKYENPEDIDKIYFYKSLIDTAEGVMIYAKRMSDYAAELAQKETNPKRKAELQKISE
HHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
INARVPAHKPSTYWEAIQAVWTIESLLVVEENQTGMSIGRVDQYMYPFYKADIEAGRMTD
HHCCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHCCHHHHCCCCCCCCC
YEAFELSGCMLIKMSEMMWITSEGGSKFFAGYQPFVNMCVGGVTREGRDATNELTYLLMD
CCHHCCCCEEEEEEECEEEEECCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHH
AVRHVKIYQPSLACRIHKSSPQKYLKKIVDVIRAGMGFPACHFDDVHIKIMLAKGVSIED
HHHHHEEECCCEEEEEECCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCC
ARDYCLMGCVEPQKSGRLYQWTSTGYTQWPICIELVLNNGVPLWYGKQVCPDMGDLSQFK
CCCHHHHCCCCCCCCCCEEEEECCCCCCCCHHEEEECCCCCCEEECHHHCCCCCCHHHHH
TYEQFEAAVKEQIKFITKWTSVATVISQRVHKELAPKPLMSMMYEGCMENGRGVEAGGAM
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCCCCCCE
YNFGPGVVWSGLATYADSMAAIKKLVFEDKKYTLQEMNEALKADFVGYEQLRKDCLEAPK
EECCCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHCCC
YGNDDDYADLIAADLINFTEQEHRKYKTLYSVLSHGTLSISNNTPFGQMTGATANGRRAW
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCEEE
MPLSDGISPSQGADFKGPTSIIKSVSKMSCEDMNIGMVHNFKLIAGLLDTPEGEQGIITL
CCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHHH
LRSACALQLGEVQFNYLDNKTLIEAQKHPDQYRDLIVRVAGYSAFFVELCKDVQDEIISR
HHHHHHHHHCCEEEEECCCCCEEHHHCCCHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHH
TMLTHF
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8265357; 9278503; 7773398 [H]