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The map label for this gene is rnhA

Identifier: 153936460

GI number: 153936460

Start: 437409

End: 437858

Strand: Direct

Name: rnhA

Synonym: CLC_0439

Alternate gene names: 153936460

Gene position: 437409-437858 (Clockwise)

Preceding gene: 153935088

Following gene: 153935454

Centisome position: 11.63

GC content: 28.0

Gene sequence:

>450_bases
ATGAAAAAAGTAATAATATACACTGATGGAGCTTGTAGAGGAAATGGGCAAGAAAATACTATAGGGGCTTATGGAATAGT
TTTAATGTATGGTGAACATAAAAAAGAAATAAAAAAAGCTTTTAGGGATACAACTAATAACATTATGGAATTATCTGCAG
TGGTAGAGGCACTTTCTTTATTAAAAGAACCTTGTAGCATAGAACTTTATAGTGATTCTGCTTATGTTATAAATGCCATA
AATCAAAAATGGCTAGATAATTGGAAGAAAAATAATTGGAAAACTGCTTCTAAATCTCCAGTTAAGAATAAAGAATTGTG
GGAAAAGCTAGATGAATTATTAAAAAAACATTCTGTTAAGTTTATAAAAGTAAAGGGTCATTCAGATAATGAGTATAATA
ATAGATGTGATAAATTAGCAAATGAAGCCATGGATGAATTTAATGTTTAA

Upstream 100 bases:

>100_bases
AATAATATTGTAGGGTTTATAAAAGAAAAATATGATGGGAAAAGTAATTTAAAAGCACATAAGCAAGAAGATAAGGCTTA
TGAGGAGGTGGCATCTGTAG

Downstream 100 bases:

>100_bases
TATTTTACAGAAATGCATATCCTACACTTAGTATTAAAAATAAATAAAGGAGAGTGTAAGATATGAACAATAGATTTTTA
TCAGGTATTGCTGCAGGAGC

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H

Number of amino acids: Translated: 149; Mature: 149

Protein sequence:

>149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLKEPCSIELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV

Sequences:

>Translated_149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLKEPCSIELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV
>Mature_149_residues
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSLLKEPCSIELYSDSAYVINAI
NQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVKFIKVKGHSDNEYNNRCDKLANEAMDEFNV

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain

Homologues:

Organism=Homo sapiens, GI21359816, Length=145, Percent_Identity=31.7241379310345, Blast_Score=76, Evalue=1e-14,
Organism=Escherichia coli, GI1786408, Length=143, Percent_Identity=47.5524475524476, Blast_Score=133, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI115532542, Length=145, Percent_Identity=32.4137931034483, Blast_Score=85, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17505482, Length=136, Percent_Identity=35.2941176470588, Blast_Score=75, Evalue=1e-14,
Organism=Drosophila melanogaster, GI45552509, Length=147, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI17137476, Length=147, Percent_Identity=33.3333333333333, Blast_Score=79, Evalue=1e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RNH_CLOB1 (A7FR34)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382783.1
- ProteinModelPortal:   A7FR34
- SMR:   A7FR34
- STRING:   A7FR34
- GeneID:   5395779
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0424
- eggNOG:   NOG69418
- HOGENOM:   HBG742437
- OMA:   NGWRTAD
- ProtClustDB:   PRK00203
- BioCyc:   CBOT441770:CLB_0424-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00042
- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156

Pfam domain/function: PF00075 RnaseH; SSF53098 RNaseH_fold

EC number: =3.1.26.4

Molecular weight: Translated: 17132; Mature: 17132

Theoretical pI: Translated: 8.77; Mature: 8.77

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSL
CCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEPCSIELYSDSAYVINAINQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVK
HCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
FIKVKGHSDNEYNNRCDKLANEAMDEFNV
EEEEECCCCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKKVIIYTDGACRGNGQENTIGAYGIVLMYGEHKKEIKKAFRDTTNNIMELSAVVEALSL
CCEEEEEECCCCCCCCCCCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LKEPCSIELYSDSAYVINAINQKWLDNWKKNNWKTASKSPVKNKELWEKLDELLKKHSVK
HCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCE
FIKVKGHSDNEYNNRCDKLANEAMDEFNV
EEEEECCCCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA