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The map label for this gene is eno

Identifier: 153936384

GI number: 153936384

Start: 265164

End: 266459

Strand: Direct

Name: eno

Synonym: CLC_0286

Alternate gene names: 153936384

Gene position: 265164-266459 (Clockwise)

Preceding gene: 153936169

Following gene: 153937200

Centisome position: 7.05

GC content: 33.56

Gene sequence:

>1296_bases
ATGAAAAATTATATTGAAATAGTAGATGTATACGCAAGACAAATCTTAGATTCAAGATGCAACCCTACAGTAGAAGTAGA
AGTAGAATTAGAAGACGGAACAGTAGGAGTAGCAGCAGTTCCATCAGGAGCTTCTACAGGAGCCTTTGAAGCAGTTGAAT
TAAGAGATGGAGATAAATCTAAATACTTAGGTAAAGGCGTTTTAAAAGCTGTAGATAATGTAAACACTATAATAGCAGAT
GAACTTGTTGGAATGAATGTTTTAGATCAAGTAGCTATTGATAAAACTATGATAGAATTAGATGGAACAGACAATAAAGC
AAAATTAGGTGCAAATGCAATGCTAGGAGTATCTTTAGCTTGTGCAAAAGCTGCAGCTAACTCTTTAGGAATGAGTTTAT
ACCAATACATAGGAGGAGTAAATGGAAAAGTTTTACCAGTACCTATGATGAACATAATAAATGGTGGAAAACATGCGGAC
AACAATGTTGACCTTCAAGAATTCATGATAATGCCAGCAGGAGCTCCTTCTTTCAGTGAAGCTTTAAGAATGTGTTCAGA
AGTATATCATGCATTAAAATCAACATTAAAAGCACAAGGATATGATACAGGGGTAGGCGACGAAGGTGGATTTGCTCCAA
ACTTAAAATCAAATGAAGAAGCTATAGTAGTTATAATAGAAGCTATAAAAAAAGCTGGATATACTCCAGGAGAAGATATA
TTCATAGCTTTAGATCCAGCTTCATCAGAAATATTTGAAGATGGAAAATACAATCTAGCAGGAGAAGGAAGAGTATTAAC
TCCAGAAGAAATGGCAAACTACTATGTAGAATTAGCAGAAAAATACCCAATAATCTCTATAGAAGACGGTATGGCAGAAG
AAGATTGGGATGGTTGGAAAATCCTTACTGAAAAAATAGGAAACAAAGTTCAATTAGTAGGAGACGATTTATTCGTTACA
AATACTGAAAGATTATCTAAAGGAATAAAATTAGGGGTTGCAAACTCAATTCTTATAAAACTTAACCAAATAGGAACATT
AACAGAAACATTAAATGCTATAGAAATGGCAGAAAGAGCTGGATATACAGCAGTAGTATCTCATAGATCAGGAGAAACTG
AAGATACAACAATAGCTGACTTAGTTGTTGCAGTAAATGCAGGACAAATAAAAACAGGTGCTCCAGCAAGATCAGAAAGA
GTTGCTAAATACAATCAATTATTAAGAATAGAAGAAGAACTTAATGATATGGGAGAATACAGAGGATTAAAAGCATTCTA
CAATATCAATAAATAA

Upstream 100 bases:

>100_bases
CTTCCTAAACCCTCTGAAATGACAGGGAATAGTCTTATTAAATAATAGGGAAACTATATTTAAGATAAAATAATTTTTTT
AAAAATAAGGAGGACCCAAC

Downstream 100 bases:

>100_bases
TAATTTTTAAAACAAGGGGCTGCTCATAGTATGGGTAGTCCTTTAATCTATAAAAATGTAAATACAAAAGTATATATGTA
ATTAAAATATAAATTAATCC

Product: phosphopyruvate hydratase

Products: NA

Alternate protein names: 2-phospho-D-glycerate hydro-lyase; 2-phosphoglycerate dehydratase

Number of amino acids: Translated: 431; Mature: 431

Protein sequence:

>431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Sequences:

>Translated_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK
>Mature_431_residues
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKSKYLGKGVLKAVDNVNTIIAD
ELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLACAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHAD
NNVDLQEFMIMPAGAPSFSEALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWKILTEKIGNKVQLVGDDLFVT
NTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERAGYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSER
VAKYNQLLRIEEELNDMGEYRGLKAFYNINK

Specific function: Catalyzes the reversible conversion of 2- phosphoglycerate into phosphoenolpyruvate. It is essential for the degradation of carbohydrates via glycolysis

COG id: COG0148

COG function: function code G; Enolase

Gene ontology:

Cell location: Cytoplasm. Secreted. Cell surface. Note=Fractions of enolase are present in both the cytoplasm and on the cell surface. The export of enolase possibly depends on the covalent binding to the substrate; once secreted, it remains attached to the bacterial ce

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enolase family

Homologues:

Organism=Homo sapiens, GI5803011, Length=430, Percent_Identity=52.3255813953488, Blast_Score=434, Evalue=1e-121,
Organism=Homo sapiens, GI4503571, Length=436, Percent_Identity=51.605504587156, Blast_Score=429, Evalue=1e-120,
Organism=Homo sapiens, GI301897477, Length=431, Percent_Identity=51.7401392111369, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897469, Length=431, Percent_Identity=51.7401392111369, Blast_Score=422, Evalue=1e-118,
Organism=Homo sapiens, GI301897479, Length=425, Percent_Identity=46.8235294117647, Blast_Score=362, Evalue=1e-100,
Organism=Homo sapiens, GI169201331, Length=354, Percent_Identity=25.7062146892655, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI169201757, Length=354, Percent_Identity=25.7062146892655, Blast_Score=100, Evalue=2e-21,
Organism=Homo sapiens, GI239744207, Length=354, Percent_Identity=25.7062146892655, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1789141, Length=424, Percent_Identity=63.4433962264151, Blast_Score=522, Evalue=1e-149,
Organism=Caenorhabditis elegans, GI71995829, Length=431, Percent_Identity=53.5962877030162, Blast_Score=423, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI17536383, Length=431, Percent_Identity=53.5962877030162, Blast_Score=422, Evalue=1e-118,
Organism=Caenorhabditis elegans, GI32563855, Length=189, Percent_Identity=48.6772486772487, Blast_Score=182, Evalue=4e-46,
Organism=Saccharomyces cerevisiae, GI6321693, Length=433, Percent_Identity=53.5796766743649, Blast_Score=414, Evalue=1e-116,
Organism=Saccharomyces cerevisiae, GI6324974, Length=442, Percent_Identity=50.9049773755656, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324969, Length=442, Percent_Identity=50.9049773755656, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6323985, Length=442, Percent_Identity=50.6787330316742, Blast_Score=405, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6321968, Length=433, Percent_Identity=52.8868360277136, Blast_Score=388, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24580918, Length=425, Percent_Identity=53.1764705882353, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580916, Length=425, Percent_Identity=53.1764705882353, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580920, Length=425, Percent_Identity=53.1764705882353, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI24580914, Length=425, Percent_Identity=53.1764705882353, Blast_Score=411, Evalue=1e-115,
Organism=Drosophila melanogaster, GI281360527, Length=418, Percent_Identity=53.5885167464115, Blast_Score=409, Evalue=1e-114,
Organism=Drosophila melanogaster, GI17137654, Length=418, Percent_Identity=53.5885167464115, Blast_Score=409, Evalue=1e-114,

Paralogues:

None

Copy number: 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase,

Swissprot (AC and ID): ENO_CLOB1 (A7FQP0)

Other databases:

- EMBL:   CP000726
- RefSeq:   YP_001382634.1
- ProteinModelPortal:   A7FQP0
- SMR:   A7FQP0
- STRING:   A7FQP0
- GeneID:   5397841
- GenomeReviews:   CP000726_GR
- KEGG:   cba:CLB_0271
- eggNOG:   COG0148
- HOGENOM:   HBG726599
- OMA:   DIAVGTN
- ProtClustDB:   PRK00077
- BioCyc:   CBOT441770:CLB_0271-MONOMER
- GO:   GO:0006096
- HAMAP:   MF_00318
- InterPro:   IPR000941
- InterPro:   IPR020810
- InterPro:   IPR020809
- InterPro:   IPR020811
- PIRSF:   PIRSF001400
- PRINTS:   PR00148
- TIGRFAMs:   TIGR01060

Pfam domain/function: PF00113 Enolase_C; PF03952 Enolase_N

EC number: =4.2.1.11

Molecular weight: Translated: 46406; Mature: 46406

Theoretical pI: Translated: 4.32; Mature: 4.32

Prosite motif: PS00164 ENOLASE

Important sites: ACT_SITE 208-208 ACT_SITE 340-340 BINDING 158-158 BINDING 167-167 BINDING 288-288 BINDING 315-315 BINDING 340-340 BINDING 391-391

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCH
KYLGKGVLKAVDNVNTIIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC
>Mature Secondary Structure
MKNYIEIVDVYARQILDSRCNPTVEVEVELEDGTVGVAAVPSGASTGAFEAVELRDGDKS
CCHHHHHHHHHHHHHHHHCCCCEEEEEEEECCCEEEEEECCCCCCCCCEEEEEECCCCCH
KYLGKGVLKAVDNVNTIIADELVGMNVLDQVAIDKTMIELDGTDNKAKLGANAMLGVSLA
HHHHHHHHHHHHCCHHHHHHHHHCHHHHHHHHHCCEEEEECCCCCCHHCCCCHHHHHHHH
CAKAAANSLGMSLYQYIGGVNGKVLPVPMMNIINGGKHADNNVDLQEFMIMPAGAPSFSE
HHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHCCCCCCCCCCCHHHEEEECCCCCCHHH
ALRMCSEVYHALKSTLKAQGYDTGVGDEGGFAPNLKSNEEAIVVIIEAIKKAGYTPGEDI
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEEEEEEHHHHHCCCCCCCCE
FIALDPASSEIFEDGKYNLAGEGRVLTPEEMANYYVELAEKYPIISIEDGMAEEDWDGWK
EEEECCCCHHHHCCCCEEECCCCCEECHHHHHHHHHHHHHHCCEEEECCCCCCCCCCHHH
ILTEKIGNKVQLVGDDLFVTNTERLSKGIKLGVANSILIKLNQIGTLTETLNAIEMAERA
HHHHHCCCEEEEEECCEEEECHHHHHCCCEEECCCEEEEEEECCCCHHHHHHHHHHHHHC
GYTAVVSHRSGETEDTTIADLVVAVNAGQIKTGAPARSERVAKYNQLLRIEEELNDMGEY
CCEEEEECCCCCCCCCCHHHEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHH
RGLKAFYNINK
CCEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA