| Definition | Clostridium botulinum A str. Hall, complete genome. |
|---|---|
| Accession | NC_009698 |
| Length | 3,760,560 |
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The map label for this gene is hsdR [H]
Identifier: 153936325
GI number: 153936325
Start: 2150284
End: 2153190
Strand: Reverse
Name: hsdR [H]
Synonym: CLC_2028
Alternate gene names: 153936325
Gene position: 2153190-2150284 (Counterclockwise)
Preceding gene: 153937419
Following gene: 153936428
Centisome position: 57.26
GC content: 24.87
Gene sequence:
>2907_bases ATGAAACCAATATTAGAAGAAACAGATATAATAGATAAACAAACGGATCAGTATATTGATCTTGAACATAAAGAAATAGA ATCAGCTGATGATAAATATTTAATAAGTAATAATCTGATAGTCAATTCAGAACAAGGAAATTTATTATGTGAAATTAAAA GATGTTTAAGTGAATGTGAAAAATTTTATTTTAGTGTGGCTTTTGTTAATTTTAGTGGTATTCAACTTTTACTAGATAGT TTTAAAGAACTTGAACAAAGAGAAATTGAAGGGAAAATAATAACATCAACTTATCTTAATTTTACTGAACCAAAAGCTCT TAAAAAAATACAAGAATTTAATAATATAGATTTAAAAGTTTTTGTTTCTAAAAAAGAGATAGGATTTCATACAAAGGCGT ATATTTTTGAGAATGAAGCAGAATATAAAATAATTATAGGATCTTCAAATATAACGCAAAGAGCTTTAAAAAGTAATATC GAGTGGAATGTTAAATCTATTTCTAAAAAGGAGAATAGTTTTACAAAAGAGGTTTTAAAAGAATATTTAAAGTTATGGGG AGAAACTTCTGTTGTTGATGATAATTTTATAAGAGAATATGATAGATTCTTAAGAGAACTAAAAAAAGTTGAAGAAAATA AAACTTTAAATTTTTTTGATTATGAGGTAATAATGCCTAATGATATGCAAAAAAGAGCTTTGGAAAATTTAAAAAGACTA AGAAAATCTGAAGAAGATAAAGCATTAGTCATTGCAGCTACGGGAACAGGTAAGACATATATGTCAGCTTTTGATGTCAT AGAATATAATCCTAAAAAAATGCTTTTTTTAGTTCATAGAGAAGAAATTTTAAGAAGGTCTGAAAAGACATTCAAAAAAT TAGTAAAAAATAAAAATAAAACAACAGGACTTTTAACTGGAAATAGTAAAGCTATAGATTCTGATTATTTGTTTTCAACT ATACAAACAATGGGAAAACAGTTCCAAAACTTTAAAAAAGATGAATTTGAATATATTGTTATAGATGAAGCTCACCATAT TTCTAGCCCATTATATGAAAGAGTTATAAATTATTTTGAACCAAAATTTTTACTTGGTATGACTGCAACACCAGAGAGAT GTGATGGGAAAAACATATTTGATATTTTTAATAATAATGTTGCTTTAGAAGTTAGACTTAGAGAGGCATTAGAACTTGAG TTAGTTATTCCTTTTCATTATTTTGGTATAACAGATATTGAAGATATTGATTTGAGTGATGTCAAAGTACAAGATTTAGA TGAAATAGCTAAAAGACTAAATGTTAATAAAAGAGTAGATTTTATAATAGACAAGATGAATTTTTATGGTCATGATGGAG AAAAGCGTAAATGTGTAGGATTTTGTGTAAATATTGATCATGCAAAATATATGGCAGATGAGTTTAATAAAAGAGGTATA CATAGTGTATTTTTAACAGGTAATAATTCTGCTAATGAGAGAGAATTGTATATAAAAAAATTAGAAGATGATAAAGATGA TTTAGAAGTAATATTTACTGTAGATATTTTTAATGAAGGGATAGATATACCATCCATTAATTTAATTTTAATGCTTAGAC CAACTAATTCTCCTATAGTATTTATACAACAACTTGGTAGAGGACTTAGAAAATATCCAAATAAAACTTTTTTAACTGTA TTAGATTTTATAGGAAATCATAATAAAACATTTTTAATAGCACTTGCTTTAAATGGTTCTAGGTATTACGATAAAGATAG TTTAAAGGTTGCCGTAGCAACACAGTTTTCTAATTTAGCAGGATGCACTAATATTCAAATGGACAGAATTTCTATAGAGA GAATACTAGAGCAATTAAATAAAGAAAATTTTAATTCTATGAAATACTTAAAAGAAGATTATATGGAGTTTAAAAAAATG AATGATGGAAAAGTTCCATATTTTCTTATGGATTATATTAGATACGATGGGGCACCAGATCCATTAAAATTTTTAACTAA AGCAAGAACTTACCTTGATTTTGTAGTAAAAATGGAAAAGGATGAAAGTTTAAAAAATTTATTAGATGAAGAGGTTTTCT TTAAAATATTAAAAGAGTTTACAGGAAAGCTACCAATAAAAAGAGTATATGAATTTAGTATATTAAAATATCTTTTGAGG CATAAAGATATAAATTTAGAAGAAGCAAAAAAAGAGATTTTAAAATATATTGAGTATGTAGATGATAACAGTATTGAGCA TGCTTTTAGCTGTTTAAATCAACAATATTATGACTCTTCCCAATTAAAAAATTCTATTAAATGTTTTAATTTAAAAGATG GAATGTTAAAAACTACTTCGGAGTTTGATACTGTAATATATGATATAAGATATAGAAAATATATAGAGGATATTATAAAT TATGGACTTATCAGGTATGAAAAAGAGTTTTCTAGCAAGTATTATGGTGTACCATTTTTTAAGTTATATGAACAGTATCA AATAATAGATACAGCTTTGCTTTCAAATTATACAAAAATTCATAGTTCATTTAGAGGAATGGGACTCATAACTAATGGAA AAGAGTATTTTCTTTTTATAGATTTACATAAGGATGATGATATTAAGGAAAGTATTAATTATAAAGATAAATTTATAGAT GATTCAAATTTTCAATGGCAAACGCCTAATAGTACATCTCAAAACTCTGAAAGAGGCAAAAATATAATATTTAATAAAGA AAGAGGAGTTAATTTACACATTTTCATTAGAAAGTATGGAAAAATTGATGGAGTAGTTCAAAAATTTATTTATATAGGAA AGGGAGATGTAATAAGTTATAAAGGAGAAAAGCCTATAACTGTTGAGATAAAATTATACAATAAGGTTCCAACATATTTA TATAATGAATTTGTTAAAAAGGTATAG
Upstream 100 bases:
>100_bases ATATTGTAAAAGACCATATAATAATATATATAAAATAATTTAATAAAGAAATTTGCATAGTTTATTATAAAAAAATACAT AGATAGTAAGAGGACCAAAT
Downstream 100 bases:
>100_bases CAATGATTTGCTATACCTTTTCTTGTACAAGTTTTTTTACAGCAGGAATGTCTGCCGGAGCCCAATCTAGGGAAAGTAAA TTTTCTCTATGTAGCCATAT
Product: restriction/helicase domain-containing protein
Products: NA
Alternate protein names: R.EcoAI [H]
Number of amino acids: Translated: 968; Mature: 968
Protein sequence:
>968_residues MKPILEETDIIDKQTDQYIDLEHKEIESADDKYLISNNLIVNSEQGNLLCEIKRCLSECEKFYFSVAFVNFSGIQLLLDS FKELEQREIEGKIITSTYLNFTEPKALKKIQEFNNIDLKVFVSKKEIGFHTKAYIFENEAEYKIIIGSSNITQRALKSNI EWNVKSISKKENSFTKEVLKEYLKLWGETSVVDDNFIREYDRFLRELKKVEENKTLNFFDYEVIMPNDMQKRALENLKRL RKSEEDKALVIAATGTGKTYMSAFDVIEYNPKKMLFLVHREEILRRSEKTFKKLVKNKNKTTGLLTGNSKAIDSDYLFST IQTMGKQFQNFKKDEFEYIVIDEAHHISSPLYERVINYFEPKFLLGMTATPERCDGKNIFDIFNNNVALEVRLREALELE LVIPFHYFGITDIEDIDLSDVKVQDLDEIAKRLNVNKRVDFIIDKMNFYGHDGEKRKCVGFCVNIDHAKYMADEFNKRGI HSVFLTGNNSANERELYIKKLEDDKDDLEVIFTVDIFNEGIDIPSINLILMLRPTNSPIVFIQQLGRGLRKYPNKTFLTV LDFIGNHNKTFLIALALNGSRYYDKDSLKVAVATQFSNLAGCTNIQMDRISIERILEQLNKENFNSMKYLKEDYMEFKKM NDGKVPYFLMDYIRYDGAPDPLKFLTKARTYLDFVVKMEKDESLKNLLDEEVFFKILKEFTGKLPIKRVYEFSILKYLLR HKDINLEEAKKEILKYIEYVDDNSIEHAFSCLNQQYYDSSQLKNSIKCFNLKDGMLKTTSEFDTVIYDIRYRKYIEDIIN YGLIRYEKEFSSKYYGVPFFKLYEQYQIIDTALLSNYTKIHSSFRGMGLITNGKEYFLFIDLHKDDDIKESINYKDKFID DSNFQWQTPNSTSQNSERGKNIIFNKERGVNLHIFIRKYGKIDGVVQKFIYIGKGDVISYKGEKPITVEIKLYNKVPTYL YNEFVKKV
Sequences:
>Translated_968_residues MKPILEETDIIDKQTDQYIDLEHKEIESADDKYLISNNLIVNSEQGNLLCEIKRCLSECEKFYFSVAFVNFSGIQLLLDS FKELEQREIEGKIITSTYLNFTEPKALKKIQEFNNIDLKVFVSKKEIGFHTKAYIFENEAEYKIIIGSSNITQRALKSNI EWNVKSISKKENSFTKEVLKEYLKLWGETSVVDDNFIREYDRFLRELKKVEENKTLNFFDYEVIMPNDMQKRALENLKRL RKSEEDKALVIAATGTGKTYMSAFDVIEYNPKKMLFLVHREEILRRSEKTFKKLVKNKNKTTGLLTGNSKAIDSDYLFST IQTMGKQFQNFKKDEFEYIVIDEAHHISSPLYERVINYFEPKFLLGMTATPERCDGKNIFDIFNNNVALEVRLREALELE LVIPFHYFGITDIEDIDLSDVKVQDLDEIAKRLNVNKRVDFIIDKMNFYGHDGEKRKCVGFCVNIDHAKYMADEFNKRGI HSVFLTGNNSANERELYIKKLEDDKDDLEVIFTVDIFNEGIDIPSINLILMLRPTNSPIVFIQQLGRGLRKYPNKTFLTV LDFIGNHNKTFLIALALNGSRYYDKDSLKVAVATQFSNLAGCTNIQMDRISIERILEQLNKENFNSMKYLKEDYMEFKKM NDGKVPYFLMDYIRYDGAPDPLKFLTKARTYLDFVVKMEKDESLKNLLDEEVFFKILKEFTGKLPIKRVYEFSILKYLLR HKDINLEEAKKEILKYIEYVDDNSIEHAFSCLNQQYYDSSQLKNSIKCFNLKDGMLKTTSEFDTVIYDIRYRKYIEDIIN YGLIRYEKEFSSKYYGVPFFKLYEQYQIIDTALLSNYTKIHSSFRGMGLITNGKEYFLFIDLHKDDDIKESINYKDKFID DSNFQWQTPNSTSQNSERGKNIIFNKERGVNLHIFIRKYGKIDGVVQKFIYIGKGDVISYKGEKPITVEIKLYNKVPTYL YNEFVKKV >Mature_968_residues MKPILEETDIIDKQTDQYIDLEHKEIESADDKYLISNNLIVNSEQGNLLCEIKRCLSECEKFYFSVAFVNFSGIQLLLDS FKELEQREIEGKIITSTYLNFTEPKALKKIQEFNNIDLKVFVSKKEIGFHTKAYIFENEAEYKIIIGSSNITQRALKSNI EWNVKSISKKENSFTKEVLKEYLKLWGETSVVDDNFIREYDRFLRELKKVEENKTLNFFDYEVIMPNDMQKRALENLKRL RKSEEDKALVIAATGTGKTYMSAFDVIEYNPKKMLFLVHREEILRRSEKTFKKLVKNKNKTTGLLTGNSKAIDSDYLFST IQTMGKQFQNFKKDEFEYIVIDEAHHISSPLYERVINYFEPKFLLGMTATPERCDGKNIFDIFNNNVALEVRLREALELE LVIPFHYFGITDIEDIDLSDVKVQDLDEIAKRLNVNKRVDFIIDKMNFYGHDGEKRKCVGFCVNIDHAKYMADEFNKRGI HSVFLTGNNSANERELYIKKLEDDKDDLEVIFTVDIFNEGIDIPSINLILMLRPTNSPIVFIQQLGRGLRKYPNKTFLTV LDFIGNHNKTFLIALALNGSRYYDKDSLKVAVATQFSNLAGCTNIQMDRISIERILEQLNKENFNSMKYLKEDYMEFKKM NDGKVPYFLMDYIRYDGAPDPLKFLTKARTYLDFVVKMEKDESLKNLLDEEVFFKILKEFTGKLPIKRVYEFSILKYLLR HKDINLEEAKKEILKYIEYVDDNSIEHAFSCLNQQYYDSSQLKNSIKCFNLKDGMLKTTSEFDTVIYDIRYRKYIEDIIN YGLIRYEKEFSSKYYGVPFFKLYEQYQIIDTALLSNYTKIHSSFRGMGLITNGKEYFLFIDLHKDDDIKESINYKDKFID DSNFQWQTPNSTSQNSERGKNIIFNKERGVNLHIFIRKYGKIDGVVQKFIYIGKGDVISYKGEKPITVEIKLYNKVPTYL YNEFVKKV
Specific function: The EcoAI enzyme recognizes 5'-GAGN(7)GTCA-3'. Subunit R is required for both nuclease and ATPase activities, but not for modification [H]
COG id: COG1061
COG function: function code KL; DNA or RNA helicases of superfamily II
Gene ontology:
Cell location: Cytoplasm [C]
Metaboloic importance: Unknown [C]
Operon status: Not Known
Operon components: None
Similarity: Contains 1 helicase C-terminal domain [H]
Homologues:
Organism=Saccharomyces cerevisiae, GI6320539, Length=383, Percent_Identity=25.3263707571802, Blast_Score=116, Evalue=2e-26,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR014001 - InterPro: IPR001650 - InterPro: IPR014021 - InterPro: IPR006935 - InterPro: IPR013670 - InterPro: IPR007409 [H]
Pfam domain/function: PF08463 EcoEI_R_C; PF04313 HSDR_N; PF04851 ResIII [H]
EC number: =3.1.21.3 [H]
Molecular weight: Translated: 113827; Mature: 113827
Theoretical pI: Translated: 6.69; Mature: 6.69
Prosite motif: PS50035 PLD
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.9 %Cys (Translated Protein) 1.9 %Met (Translated Protein) 2.8 %Cys+Met (Translated Protein) 0.9 %Cys (Mature Protein) 1.9 %Met (Mature Protein) 2.8 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MKPILEETDIIDKQTDQYIDLEHKEIESADDKYLISNNLIVNSEQGNLLCEIKRCLSECE CCCCCCHHHCCCCCCCCEECCCHHHHCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHH KFYFSVAFVNFSGIQLLLDSFKELEQREIEGKIITSTYLNFTEPKALKKIQEFNNIDLKV HHHEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEE FVSKKEIGFHTKAYIFENEAEYKIIIGSSNITQRALKSNIEWNVKSISKKENSFTKEVLK EEECCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH EYLKLWGETSVVDDNFIREYDRFLRELKKVEENKTLNFFDYEVIMPNDMQKRALENLKRL HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHH RKSEEDKALVIAATGTGKTYMSAFDVIEYNPKKMLFLVHREEILRRSEKTFKKLVKNKNK HCCCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC TTGLLTGNSKAIDSDYLFSTIQTMGKQFQNFKKDEFEYIVIDEAHHISSPLYERVINYFE CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCHHHHHHHHHCC PKFLLGMTATPERCDGKNIFDIFNNNVALEVRLREALELELVIPFHYFGITDIEDIDLSD CCEEEECCCCCCCCCCCCCHHHHCCCEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCC VKVQDLDEIAKRLNVNKRVDFIIDKMNFYGHDGEKRKCVGFCVNIDHAKYMADEFNKRGI CCHHHHHHHHHHCCCCCCHHHEEECHHHCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCC HSVFLTGNNSANERELYIKKLEDDKDDLEVIFTVDIFNEGIDIPSINLILMLRPTNSPIV EEEEEECCCCCCCHHHHHEECCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCEE FIQQLGRGLRKYPNKTFLTVLDFIGNHNKTFLIALALNGSRYYDKDSLKVAVATQFSNLA EHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCEEEEEEECHHCCC GCTNIQMDRISIERILEQLNKENFNSMKYLKEDYMEFKKMNDGKVPYFLMDYIRYDGAPD CCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC PLKFLTKARTYLDFVVKMEKDESLKNLLDEEVFFKILKEFTGKLPIKRVYEFSILKYLLR HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HKDINLEEAKKEILKYIEYVDDNSIEHAFSCLNQQYYDSSQLKNSIKCFNLKDGMLKTTS HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCH EFDTVIYDIRYRKYIEDIINYGLIRYEKEFSSKYYGVPFFKLYEQYQIIDTALLSNYTKI HHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH HSSFRGMGLITNGKEYFLFIDLHKDDDIKESINYKDKFIDDSNFQWQTPNSTSQNSERGK HHHHCCCEEEECCCEEEEEEEECCCCCHHHHCCCCHHCCCCCCCEEECCCCCCCCCCCCC NIIFNKERGVNLHIFIRKYGKIDGVVQKFIYIGKGDVISYKGEKPITVEIKLYNKVPTYL CEEEECCCCCEEEEEEEECCCHHHHHHHHHEECCCCEEEECCCCCEEEEEEEECCCHHHH YNEFVKKV HHHHHHCC >Mature Secondary Structure MKPILEETDIIDKQTDQYIDLEHKEIESADDKYLISNNLIVNSEQGNLLCEIKRCLSECE CCCCCCHHHCCCCCCCCEECCCHHHHCCCCCCEEEECCEEEECCCCCCHHHHHHHHHHHH KFYFSVAFVNFSGIQLLLDSFKELEQREIEGKIITSTYLNFTEPKALKKIQEFNNIDLKV HHHEEEEEEECCHHHHHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHCCCCCEEE FVSKKEIGFHTKAYIFENEAEYKIIIGSSNITQRALKSNIEWNVKSISKKENSFTKEVLK EEECCCCCCEEEEEEEECCCCEEEEECCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH EYLKLWGETSVVDDNFIREYDRFLRELKKVEENKTLNFFDYEVIMPNDMQKRALENLKRL HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCEEEEEEEEECCCCHHHHHHHHHHHH RKSEEDKALVIAATGTGKTYMSAFDVIEYNPKKMLFLVHREEILRRSEKTFKKLVKNKNK HCCCCCCEEEEEECCCCCHHHHHHHHHEECCCCEEEEEEHHHHHHHHHHHHHHHHHCCCC TTGLLTGNSKAIDSDYLFSTIQTMGKQFQNFKKDEFEYIVIDEAHHISSPLYERVINYFE CEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCHHCCCHHHHHHHHHCC PKFLLGMTATPERCDGKNIFDIFNNNVALEVRLREALELELVIPFHYFGITDIEDIDLSD CCEEEECCCCCCCCCCCCCHHHHCCCEEEEEEECCCCEEEEEEEEEECCCCCCCCCCCCC VKVQDLDEIAKRLNVNKRVDFIIDKMNFYGHDGEKRKCVGFCVNIDHAKYMADEFNKRGI CCHHHHHHHHHHCCCCCCHHHEEECHHHCCCCCCCCEEEEEEEECCHHHHHHHHHCCCCC HSVFLTGNNSANERELYIKKLEDDKDDLEVIFTVDIFNEGIDIPSINLILMLRPTNSPIV EEEEEECCCCCCCHHHHHEECCCCCCCEEEEEEEEEECCCCCCCCEEEEEEEECCCCCEE FIQQLGRGLRKYPNKTFLTVLDFIGNHNKTFLIALALNGSRYYDKDSLKVAVATQFSNLA EHHHHHHHHHHCCCHHHHHHHHHHCCCCCEEEEEEEECCCEEECCCCEEEEEEECHHCCC GCTNIQMDRISIERILEQLNKENFNSMKYLKEDYMEFKKMNDGKVPYFLMDYIRYDGAPD CCCCCEEHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCC PLKFLTKARTYLDFVVKMEKDESLKNLLDEEVFFKILKEFTGKLPIKRVYEFSILKYLLR HHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH HKDINLEEAKKEILKYIEYVDDNSIEHAFSCLNQQYYDSSQLKNSIKCFNLKDGMLKTTS HCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHHHHHCCCEEEECCCCCCCCCH EFDTVIYDIRYRKYIEDIINYGLIRYEKEFSSKYYGVPFFKLYEQYQIIDTALLSNYTKI HHHHHHHHHHHHHHHHHHHHCCCEEEEHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH HSSFRGMGLITNGKEYFLFIDLHKDDDIKESINYKDKFIDDSNFQWQTPNSTSQNSERGK HHHHCCCEEEECCCEEEEEEEECCCCCHHHHCCCCHHCCCCCCCEEECCCCCCCCCCCCC NIIFNKERGVNLHIFIRKYGKIDGVVQKFIYIGKGDVISYKGEKPITVEIKLYNKVPTYL CEEEECCCCCEEEEEEEECCCHHHHHHHHHEECCCCEEEECCCCCEEEEEEEECCCHHHH YNEFVKKV HHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 8412658 [H]