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The map label for this gene is adh1 [H]

Identifier: 153936239

GI number: 153936239

Start: 2192186

End: 2193328

Strand: Reverse

Name: adh1 [H]

Synonym: CLC_2068

Alternate gene names: 153936239

Gene position: 2193328-2192186 (Counterclockwise)

Preceding gene: 153935947

Following gene: 153934931

Centisome position: 58.32

GC content: 33.77

Gene sequence:

>1143_bases
ATGAAAGAATTTAGTATTAATACAGATGTCTATTTTGGAGAAGGATCTTTAGATAGACTAAAGGAAATTAAAAATAAAAG
AGTACTAATTGTATGTGATAAATTTATGGAAACTTCAGGTATGGCTGCAAAAGTACAACAAAAACTTGTTGATTGTGAAG
TAGCTATATATAGCGATATTGTACCTGATCCATCTGTAGAAGTTATTGCATCTGGTATTCAAAAACTTCAAAGTTGTAAT
GCAGAGGTTATGATAGCTCTTGGTGGAGGTTCATCAATTGATGGAGCTAAGGCCATGAGAGAATATGCCAAAAAAATTAC
TGGAGGAGTTTCCACTATAGAAGAATTTTATGCTATACCTACTACAAGTGGTACAGGCTCTGAAGTAACTGAATATGCAG
TAATTACAAACAAACAAGAAGGATTAAAGTATGCTCTTACAGATAAATCACTTCTTCCTACGGTTGCTATTCTTGACCCT
GAGTTGGTTAAATCTGTACCCAAAGCTATAACTGCCGATACAGGAATGGATGTAATCACACATGCCATGGAGGCCTATGT
TTCAAAAAATGCTACTGATTTTTCAGATGCTCTTGCAGAAAAAGCCTTCACTTTAGCTTTTAGATTTTTACCACAGGCTT
ATGCTGATGGAAATGATATAATAGCAAGGGAAAAACTTCACAATGCATCATGCCTTGCTGGTATGGCTTTTAATGCAGCT
GGACTTGGAATTACTCATAGTTTAGCTCATGCAGTGGGTGGAAAGTTACATATTTCCCATGGAAGAAGTAATGCAATGAT
ACTTCCTTATGTAGTTGAATATAATGCTAATTTAAATAAAGAGACCTTTAATGTTGAATATGACGTGGCAGCTAAGAAGT
ATCAAAGACTTGCCAAATTATTGAAATTGCATGCGCCAAATGTAAATATTGGTGTTAGCAATCTAATTAAAAGTATTGTA
GAACTTCAAAATACATTAATGATTCCAACTACATTAAAAGAACAAGGTACTGATATGGCTTTAGCTGAAGCATCAAGAGA
AGAGATTGTTAATGTAGCTCTTAAGGATGTATGCACAACAGCAAATCCTAGAGAAGTTACAAAGGAAGATTTATTAAAAA
TTTTAGATAAAGTTTTAGAATAG

Upstream 100 bases:

>100_bases
ACTTAGTTTGATTGTTATAATTGTAGGTTAATTTTATATTTAAGTTAAAGAATACCAAATTCATTCATATCTTGATATTT
TTTTATAGGAGAAAATGCAT

Downstream 100 bases:

>100_bases
TATATATTTTATACCACTCATTAATGGGTGGTATTTTGTTCAAATTTTCAGAAAAGTTGAACAATTAAATAGAATAAAAT
TTAATTTATAATATAAAAAA

Product: alcohol dehydrogenase, iron-containing

Products: aldehyde; ketone; NADH; H+

Alternate protein names: BDH [H]

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAAKVQQKLVDCEVAIYSDIVPDPSVEVIASGIQKLQSCN
AEVMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAMILPYVVEYNANLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVSNLIKSIV
ELQNTLMIPTTLKEQGTDMALAEASREEIVNVALKDVCTTANPREVTKEDLLKILDKVLE

Sequences:

>Translated_380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAAKVQQKLVDCEVAIYSDIVPDPSVEVIASGIQKLQSCN
AEVMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAMILPYVVEYNANLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVSNLIKSIV
ELQNTLMIPTTLKEQGTDMALAEASREEIVNVALKDVCTTANPREVTKEDLLKILDKVLE
>Mature_380_residues
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAAKVQQKLVDCEVAIYSDIVPDPSVEVIASGIQKLQSCN
AEVMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIPTTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDP
ELVKSVPKAITADTGMDVITHAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
GLGITHSLAHAVGGKLHISHGRSNAMILPYVVEYNANLNKETFNVEYDVAAKKYQRLAKLLKLHAPNVNIGVSNLIKSIV
ELQNTLMIPTTLKEQGTDMALAEASREEIVNVALKDVCTTANPREVTKEDLLKILDKVLE

Specific function: This enzyme has activity using butanol and ethanol as substrates [H]

COG id: COG1454

COG function: function code C; Alcohol dehydrogenase, class IV

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the iron-containing alcohol dehydrogenase family [H]

Homologues:

Organism=Homo sapiens, GI133922590, Length=311, Percent_Identity=24.4372990353698, Blast_Score=85, Evalue=1e-16,
Organism=Escherichia coli, GI1787493, Length=399, Percent_Identity=37.0927318295739, Blast_Score=235, Evalue=4e-63,
Organism=Escherichia coli, GI87082107, Length=392, Percent_Identity=33.6734693877551, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI48994951, Length=373, Percent_Identity=34.8525469168901, Blast_Score=199, Evalue=2e-52,
Organism=Escherichia coli, GI1789163, Length=388, Percent_Identity=31.9587628865979, Blast_Score=195, Evalue=4e-51,
Organism=Escherichia coli, GI1789386, Length=290, Percent_Identity=26.551724137931, Blast_Score=84, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17537053, Length=284, Percent_Identity=25.3521126760563, Blast_Score=100, Evalue=1e-21,
Organism=Saccharomyces cerevisiae, GI6321181, Length=393, Percent_Identity=36.6412213740458, Blast_Score=216, Evalue=5e-57,
Organism=Drosophila melanogaster, GI24657991, Length=380, Percent_Identity=24.2105263157895, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001670
- InterPro:   IPR018211 [H]

Pfam domain/function: PF00465 Fe-ADH [H]

EC number: 1.1.1.1

Molecular weight: Translated: 41154; Mature: 41154

Theoretical pI: Translated: 5.49; Mature: 5.49

Prosite motif: PS00913 ADH_IRON_1 ; PS00687 ALDEHYDE_DEHYDR_GLU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAAKVQQKLVDCEVAIYSDI
CCCEECCCCEEECCCCHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VPDPSVEVIASGIQKLQSCNAEVMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIP
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
TTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDPELVKSVPKAITADTGMDVIT
CCCCCCCHHHHEEEEECCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHH
HAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCC
GLGITHSLAHAVGGKLHISHGRSNAMILPYVVEYNANLNKETFNVEYDVAAKKYQRLAKL
CCCHHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHH
LKLHAPNVNIGVSNLIKSIVELQNTLMIPTTLKEQGTDMALAEASREEIVNVALKDVCTT
HHHCCCCCCCCHHHHHHHHHHHHHCEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
ANPREVTKEDLLKILDKVLE
CCCHHCCHHHHHHHHHHHCC
>Mature Secondary Structure
MKEFSINTDVYFGEGSLDRLKEIKNKRVLIVCDKFMETSGMAAKVQQKLVDCEVAIYSDI
CCCEECCCCEEECCCCHHHHHHHCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCC
VPDPSVEVIASGIQKLQSCNAEVMIALGGGSSIDGAKAMREYAKKITGGVSTIEEFYAIP
CCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCHHHHHHHHCCC
TTSGTGSEVTEYAVITNKQEGLKYALTDKSLLPTVAILDPELVKSVPKAITADTGMDVIT
CCCCCCCHHHHEEEEECCCCCCEEEECCCCCCCEEECCCHHHHHHHHHHHHCCCCHHHHH
HAMEAYVSKNATDFSDALAEKAFTLAFRFLPQAYADGNDIIAREKLHNASCLAGMAFNAA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHCCCHHHHHHHHCCC
GLGITHSLAHAVGGKLHISHGRSNAMILPYVVEYNANLNKETFNVEYDVAAKKYQRLAKL
CCCHHHHHHHHCCCEEEEECCCCCCEEEEEEEEECCCCCCEEEEEEEHHHHHHHHHHHHH
LKLHAPNVNIGVSNLIKSIVELQNTLMIPTTLKEQGTDMALAEASREEIVNVALKDVCTT
HHHCCCCCCCCHHHHHHHHHHHHHCEECCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCC
ANPREVTKEDLLKILDKVLE
CCCHHCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): 1000 [C]

Specific activity: NA

Km value (mM): 0.15 {ethanol}} 7.05 {acetaldehyde}} 0.1 {acetaldehyde}} [C]

Substrates: alcohol; NAD+

Specific reaction: an alcohol + NAD+ = an aldehyde or ketone + NADH + H+

General reaction: Redox reaction [C]

Inhibitor: 8-Hydroxy quinoline-5-sulfonic acid; Dipicolinic acid; EDTA; Ethanol; GSH; Heptafluorbutanol; Imidazole; Iodoacetamide; Iodoacetate; Isobutyramide EDTA; NAD+; NADH; NEM; o-Phenanthroline of Zn2+; PCMB; Pyrazole; Substituted pyrazoles 4-iodo-; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2673928 [H]