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The map label for this gene is nusA [H]

Identifier: 153936194

GI number: 153936194

Start: 2395580

End: 2396953

Strand: Reverse

Name: nusA [H]

Synonym: CLC_2268

Alternate gene names: 153936194

Gene position: 2396953-2395580 (Counterclockwise)

Preceding gene: 153935745

Following gene: 153937518

Centisome position: 63.74

GC content: 30.13

Gene sequence:

>1374_bases
ATGAACCAGGAATTTGTTGAAGCATTAAGGGAAATAGTAAAAGAAAAAGGTATAAGTGCAGATCTTTTGTTTACAACTAT
AGAGGATGCTTTAGTAACAGCATATAAGAAAAATTATGCAAAGCAAGGCGGATCAACTAATAATGTAAAGGTAATAATGA
ACAGGGAAAATGGAGAAATAAAAGTATATGCTCAAAAGAAAGTAGTAGATTTTGTAGAAGAGGAAGTTGAGGAAATATCC
TTAGAAGATGCTAAAGAAATCGATCCAAACTATGAATTAGAAGATATAATAAATATTGAAGTAACACCTAAAAAGTTTGG
AAGAATAGCAGCTCAAGCAGCTAAACAGGTAGTTATACAAAGAATAAAAGAAGAAGAAAGAAGAATTGTATACAACGAAT
ATATAGAAAAAGAAGAAGATATATTAACAGGAACAGTTCTAAGAAAGGATAAGGGAAATATCCTTATAAACGTAGGTAAG
TCAGAGGCGGTTTTAGGGCCAAATGAGCAAATACCTGGAGAACAATTTAGATTTAATGAAAAAATAAAATTATATGTAGT
AGAAGTAAAAAATACTACAAAGGGACCACAGGTTCTAATATCAAGAACTCATCCAGGCTTAGTAAAAAGGTTATTTGAAC
TTGAAGTTCCAGAAATATATAATGGAATAGTAGAGATAAAAAGTATAGCAAGAGAAGCAGGATCAAGAACTAAAATAGCA
GTATATTCTAATGATGAATCGGTGGATCCAATGGGTGCTTGTGTTGGGCCTAAAGGTGTAAGGGTTCAAAATATAGTAAA
TGAGCTTAAAAATGAAAAAATAGACATAATAAAATGGAGCAAATTCCCAGATGAACTTATATGCAATGCTTTAAGTCCAG
CTAAAGTAATTGATGTAACTATAGTGGATGAAGAAAATAAAGCTGCAAGGGCTGTAGTAGATGATAGTCAGTTATCTTTA
GCTATAGGTAAAGAGGGTCAAAATGTTAGACTAGCAGCTAAGTTAACAGGCTGGAAAATAGACATAAAAAGTAAATCACA
AGCAGAAAAAGAAGCAACATTAAATTTATCTGAAGCTAAAGATGAAAAAATCTTAGAGAATAAAAAAGAAGATAAAGAGG
ACAAAATAGATTTACATGAAGAAATGGATAGCATAGTTGAAGATACAAAAGAAAATATAAAAGAAGAGATAAGTGTATCT
CACGATAATCAAGAAGTAGATTCAGAGTTAGAAAAGGCCATGGAAGAAGTTTTTAATACTGAAGATAAAGAAAATAAATT
AGAAAAAGATATGAATTTATTTGAAGATGATAATGAAAAACCAAGTATAGACACAGAAAAAGCTATAGAAGAAATCTTTA
AAGATGAATTTTAA

Upstream 100 bases:

>100_bases
GAGATAGATATAAAGGTTGAAGAAAACATAGTAACTATACCTAGAGAAAAAATATCAAAGACTACTTTAAAAGGGGAACT
GTAAGGAGGTTAATAAAAAA

Downstream 100 bases:

>100_bases
TAGAATAAAAAAATCAAAAATAATTATATAACAAATATAATAAAGCAGGTGATTAAAGGATGAAAGCTAAAAAAATACCT
TTAAGAATGTGCACAGGATG

Product: transcription elongation factor NusA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 457; Mature: 457

Protein sequence:

>457_residues
MNQEFVEALREIVKEKGISADLLFTTIEDALVTAYKKNYAKQGGSTNNVKVIMNRENGEIKVYAQKKVVDFVEEEVEEIS
LEDAKEIDPNYELEDIINIEVTPKKFGRIAAQAAKQVVIQRIKEEERRIVYNEYIEKEEDILTGTVLRKDKGNILINVGK
SEAVLGPNEQIPGEQFRFNEKIKLYVVEVKNTTKGPQVLISRTHPGLVKRLFELEVPEIYNGIVEIKSIAREAGSRTKIA
VYSNDESVDPMGACVGPKGVRVQNIVNELKNEKIDIIKWSKFPDELICNALSPAKVIDVTIVDEENKAARAVVDDSQLSL
AIGKEGQNVRLAAKLTGWKIDIKSKSQAEKEATLNLSEAKDEKILENKKEDKEDKIDLHEEMDSIVEDTKENIKEEISVS
HDNQEVDSELEKAMEEVFNTEDKENKLEKDMNLFEDDNEKPSIDTEKAIEEIFKDEF

Sequences:

>Translated_457_residues
MNQEFVEALREIVKEKGISADLLFTTIEDALVTAYKKNYAKQGGSTNNVKVIMNRENGEIKVYAQKKVVDFVEEEVEEIS
LEDAKEIDPNYELEDIINIEVTPKKFGRIAAQAAKQVVIQRIKEEERRIVYNEYIEKEEDILTGTVLRKDKGNILINVGK
SEAVLGPNEQIPGEQFRFNEKIKLYVVEVKNTTKGPQVLISRTHPGLVKRLFELEVPEIYNGIVEIKSIAREAGSRTKIA
VYSNDESVDPMGACVGPKGVRVQNIVNELKNEKIDIIKWSKFPDELICNALSPAKVIDVTIVDEENKAARAVVDDSQLSL
AIGKEGQNVRLAAKLTGWKIDIKSKSQAEKEATLNLSEAKDEKILENKKEDKEDKIDLHEEMDSIVEDTKENIKEEISVS
HDNQEVDSELEKAMEEVFNTEDKENKLEKDMNLFEDDNEKPSIDTEKAIEEIFKDEF
>Mature_457_residues
MNQEFVEALREIVKEKGISADLLFTTIEDALVTAYKKNYAKQGGSTNNVKVIMNRENGEIKVYAQKKVVDFVEEEVEEIS
LEDAKEIDPNYELEDIINIEVTPKKFGRIAAQAAKQVVIQRIKEEERRIVYNEYIEKEEDILTGTVLRKDKGNILINVGK
SEAVLGPNEQIPGEQFRFNEKIKLYVVEVKNTTKGPQVLISRTHPGLVKRLFELEVPEIYNGIVEIKSIAREAGSRTKIA
VYSNDESVDPMGACVGPKGVRVQNIVNELKNEKIDIIKWSKFPDELICNALSPAKVIDVTIVDEENKAARAVVDDSQLSL
AIGKEGQNVRLAAKLTGWKIDIKSKSQAEKEATLNLSEAKDEKILENKKEDKEDKIDLHEEMDSIVEDTKENIKEEISVS
HDNQEVDSELEKAMEEVFNTEDKENKLEKDMNLFEDDNEKPSIDTEKAIEEIFKDEF

Specific function: Participates in both the termination and antitermination of transcription. Interacts with RNA polymerase and binds RNA [H]

COG id: COG0195

COG function: function code K; Transcription elongation factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S1 motif domain [H]

Homologues:

Organism=Escherichia coli, GI1789560, Length=362, Percent_Identity=36.7403314917127, Blast_Score=252, Evalue=3e-68,

Paralogues:

None

Copy number: 340 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1612 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004087
- InterPro:   IPR015946
- InterPro:   IPR009019
- InterPro:   IPR004088
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR003029
- InterPro:   IPR022967
- InterPro:   IPR013735
- InterPro:   IPR010213 [H]

Pfam domain/function: PF08529 NusA_N; PF00575 S1 [H]

EC number: NA

Molecular weight: Translated: 51872; Mature: 51872

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS50084 KH_TYPE_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNQEFVEALREIVKEKGISADLLFTTIEDALVTAYKKNYAKQGGSTNNVKVIMNRENGEI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
KVYAQKKVVDFVEEEVEEISLEDAKEIDPNYELEDIINIEVTPKKFGRIAAQAAKQVVIQ
EEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
RIKEEERRIVYNEYIEKEEDILTGTVLRKDKGNILINVGKSEAVLGPNEQIPGEQFRFNE
HHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCEEEEECCCCCEECCCCCCCCHHEEECC
KIKLYVVEVKNTTKGPQVLISRTHPGLVKRLFELEVPEIYNGIVEIKSIAREAGSRTKIA
EEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
VYSNDESVDPMGACVGPKGVRVQNIVNELKNEKIDIIKWSKFPDELICNALSPAKVIDVT
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCEEEEEE
IVDEENKAARAVVDDSQLSLAIGKEGQNVRLAAKLTGWKIDIKSKSQAEKEATLNLSEAK
EECCCCCCHHEEECCCCEEEEECCCCCCEEEEEEEECEEEEECCCCCHHHHHHCCCHHHH
DEKILENKKEDKEDKIDLHEEMDSIVEDTKENIKEEISVSHDNQEVDSELEKAMEEVFNT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
EDKENKLEKDMNLFEDDNEKPSIDTEKAIEEIFKDEF
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MNQEFVEALREIVKEKGISADLLFTTIEDALVTAYKKNYAKQGGSTNNVKVIMNRENGEI
CCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCEE
KVYAQKKVVDFVEEEVEEISLEDAKEIDPNYELEDIINIEVTPKKFGRIAAQAAKQVVIQ
EEEEHHHHHHHHHHHHHHCCCHHHHHCCCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
RIKEEERRIVYNEYIEKEEDILTGTVLRKDKGNILINVGKSEAVLGPNEQIPGEQFRFNE
HHHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCEEEEECCCCCEECCCCCCCCHHEEECC
KIKLYVVEVKNTTKGPQVLISRTHPGLVKRLFELEVPEIYNGIVEIKSIAREAGSRTKIA
EEEEEEEEECCCCCCCEEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCEEE
VYSNDESVDPMGACVGPKGVRVQNIVNELKNEKIDIIKWSKFPDELICNALSPAKVIDVT
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCEEEEEECCCCHHHHHCCCCCCEEEEEE
IVDEENKAARAVVDDSQLSLAIGKEGQNVRLAAKLTGWKIDIKSKSQAEKEATLNLSEAK
EECCCCCCHHEEECCCCEEEEECCCCCCEEEEEEEECEEEEECCCCCHHHHHHCCCHHHH
DEKILENKKEDKEDKIDLHEEMDSIVEDTKENIKEEISVSHDNQEVDSELEKAMEEVFNT
HHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCC
EDKENKLEKDMNLFEDDNEKPSIDTEKAIEEIFKDEF
CCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]