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The map label for this gene is tilS [H]

Identifier: 153936096

GI number: 153936096

Start: 3643059

End: 3644465

Strand: Reverse

Name: tilS [H]

Synonym: CLC_3490

Alternate gene names: 153936096

Gene position: 3644465-3643059 (Counterclockwise)

Preceding gene: 153935483

Following gene: 153937274

Centisome position: 96.91

GC content: 24.16

Gene sequence:

>1407_bases
ATGAAAGATGTGGTTATAAATACTATAAAAAAATATAATATGATTGAAGCTAATGACAAAATAATAGTAGGAGTATCCGG
CGGACCAGATTCTATGTGTCTTTTACATATGTTATGTTTATTAAAAAATCAATTAAGAATAAAAGATATATATGTGGCTC
ATATAAATCATGGTGTTAGAGGAGCAGAATCAGATGCTGATGAAAAATATGTAGAGAATTTTTGTTATACGAATAATTTA
GGATTTTTTTCTAAAACAATAAATATGGATAAGATAGCTAAAGAAAAAGGAATTTCCAGTGAAAGTGCAGGAAGAGAAGC
AAGATATGATTTTTTTAACTATTTGAGAAAAGAACTAGGTGCTCAAAAAATAGCAACAGCTCATAATGCAAATGATCAAG
CAGAAACTGTACTTATGAGAATAATGCGAGGAACAGGATTACAGGGATTAATAGGTATAAACCCAATAAGAGATGATTTA
TATATAAGACCTTTAATAAATGTATTAAGAGAAGATATAGAAAATTATTGTGATAAATATGAGCTAAATCCTAGAATAGA
TAAGACAAATTTACAAAGCATATATACAAGAAATAGAATTAGATTAGAATTGATACCTTATATAAAAGATAATTTTAATG
AAGATATAGTAAATACTTTATGTAGATTTTCTAATATAGTTTCTAAAGACAATAACTATTTAGAAGAAGTATCAAAAGAT
AAATTTGAAAGATATTGTACTAAAAAATTACAAAGGGTAATAATAGATAAGAGAGCTTTTTTAGAGCATGAAAGTATTTC
AACTAGAATATTAAGAGAGGCTATACTTTATATAAACCAAAACTTATATAATCTTGAAATGAGGAATATATATGATGTTT
TAGAATTAAGTTTAAATACAACAGGTAAATTTGTAAACTTGCCTAGCAATATAAAAGCTGAAAATATTTATGGAGATATA
CATTTATATAAAGAAGATAATTTTATAAAGAAGGTAAATAATGTTCAGTGTGAACTTAAAATAGGATTTAATCAAGTAGA
AGATTTAAATATTAATTTAAAAATATATTCTATAAAAGATCATAATTATAATATAAATGATAATAAATATATACAGTATT
TTGATTATGATAAAGTAAGTAATGAAAAAATTTATTTAAGAAATAGAAAAAATGGAGATAAGTTTACTCCTTTAGGGATG
AAAGGTAGTAAAAAATTAAAAGATTTTTTTATAGATTCAAAAATACCCAGAGAGCAAAGAGATAAGTTAAAACTTGTATG
TTTTGGGGAAGAAATAGCTTGGATTATAGGTTGTAGAACAGGCAATAATTTTAAAATAGATAAAAATACAAAGAATGTAT
TAGAAATAACTGTTGAAAGAGGGGAAGCAAATGGATTTGATAAGTAG

Upstream 100 bases:

>100_bases
AAATATTCTCTTTTTTTTATATAAAACCCTGCTAAAAGTATAATGATTAGAGTATTTTTATGGTATAATGTTTTAATATA
AAGGTATAAGGTGTGTTTTT

Downstream 100 bases:

>100_bases
GGACATAGAAAAAATACTTATATCTGAAGAACAAATACAAAGCAAAATAAATGAATTAGGAGATCAAATCAGTAAGGATT
ATGATGGTAAAAATTTAATG

Product: tRNA(Ile)-lysidine synthetase

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 468; Mature: 468

Protein sequence:

>468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNRIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNTTGKFVNLPSNIKAENIYGDI
HLYKEDNFIKKVNNVQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK

Sequences:

>Translated_468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNRIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNTTGKFVNLPSNIKAENIYGDI
HLYKEDNFIKKVNNVQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
>Mature_468_residues
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVRGAESDADEKYVENFCYTNNL
GFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELGAQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDL
YIRPLINVLREDIENYCDKYELNPRIDKTNLQSIYTRNRIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNTTGKFVNLPSNIKAENIYGDI
HLYKEDNFIKKVNNVQCELKIGFNQVEDLNINLKIYSIKDHNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGM
KGSKKLKDFFIDSKIPREQRDKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=200, Percent_Identity=30, Blast_Score=92, Evalue=9e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012796
- InterPro:   IPR012795
- InterPro:   IPR020825
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3; PF11734 TilS_C [H]

EC number: NA

Molecular weight: Translated: 54592; Mature: 54592

Theoretical pI: Translated: 8.66; Mature: 8.66

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVR
CCCHHHHHHHHCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
GAESDADEKYVENFCYTNNLGFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELG
CCCCCHHHHHHHHHHEECCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
AQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDLYIRPLINVLREDIENYCDKY
HHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ELNPRIDKTNLQSIYTRNRIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
CCCCCCCCCHHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCC
TGKFVNLPSNIKAENIYGDIHLYKEDNFIKKVNNVQCELKIGFNQVEDLNINLKIYSIKD
CCCEEECCCCCCCCCEEEEEEEEECCCHHHHHCCEEEEEEECCCCEEEEEEEEEEEEEEC
HNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGMKGSKKLKDFFIDSKIPREQR
CCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
CCEEEEEECCCEEEEEEEECCCCEEEECCCCEEEEEEEECCCCCCCCC
>Mature Secondary Structure
MKDVVINTIKKYNMIEANDKIIVGVSGGPDSMCLLHMLCLLKNQLRIKDIYVAHINHGVR
CCCHHHHHHHHCCEEECCCEEEEEECCCCHHHHHHHHHHHHHCCCEEEEEEEEEECCCCC
GAESDADEKYVENFCYTNNLGFFSKTINMDKIAKEKGISSESAGREARYDFFNYLRKELG
CCCCCHHHHHHHHHHEECCCCCHHHHCCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHC
AQKIATAHNANDQAETVLMRIMRGTGLQGLIGINPIRDDLYIRPLINVLREDIENYCDKY
HHHHHHHCCCCHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCC
ELNPRIDKTNLQSIYTRNRIRLELIPYIKDNFNEDIVNTLCRFSNIVSKDNNYLEEVSKD
CCCCCCCCCHHHHHHHCCCEEEEEECHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHH
KFERYCTKKLQRVIIDKRAFLEHESISTRILREAILYINQNLYNLEMRNIYDVLELSLNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHCCC
TGKFVNLPSNIKAENIYGDIHLYKEDNFIKKVNNVQCELKIGFNQVEDLNINLKIYSIKD
CCCEEECCCCCCCCCEEEEEEEEECCCHHHHHCCEEEEEEECCCCEEEEEEEEEEEEEEC
HNYNINDNKYIQYFDYDKVSNEKIYLRNRKNGDKFTPLGMKGSKKLKDFFIDSKIPREQR
CCCCCCCCCEEEEECCCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
DKLKLVCFGEEIAWIIGCRTGNNFKIDKNTKNVLEITVERGEANGFDK
CCEEEEEECCCEEEEEEEECCCCEEEECCCCEEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12552129 [H]